The document describes a study that developed a herbal mixture to study its effects on insulin resistance in diabetes patients. The mixture contained amla powder, clove powder, hing powder, and cinnamon. It was administered to an experimental group for 3 months, while a control group received no treatment. Results showed the mixture was accepted by patients. For males in the experimental group, there were significant decreases in fasting blood glucose, HbA1c, and BMI compared to the control group. However, females did not see significant changes. The herbal mixture thus had some beneficial effects on diabetic male patients.

1. P O O R V I A N A N D A N
M . S C . C L I N I C A L N U T R I T I O N A N D D I E T E T I C S – 2
2 0 1 7 – 2 0 1 8
G U I D E
A N U R A D H A S H E K H A R
DEVELOPMENT A HERBAL MIX AND STUDY IT’S EFFECT ON INSULIN
RESISTANCE (IR), IN DIABETES MELLITUS (DM) PATIENTS

2. AIM
To develop a herbal mix and study it’s effect on insulin resistance
(IR), in diabetes mellitus (DM) patients

3. OBJECTIVES
 To develop and standardize an- Herbal mix and to see if it is accepted
by patients.
 To see the effect of Herbal mix on Insulin resistance through a pre and
post-test [IR - Insulin Resistance and other blood parameters like
Fasting Blood Glucose (FBS), Fasting insulin (FI), Oral Glucose
Tolerance Test (OGTT), HBA1c, Beta cell count.]
 To observe the effects of the mix on various anthropometric parameters
and body composition.

4. METHODOLOGYIdentification of variables
and Sampling[no]
Malad
Standardization of Herbal Mixture
Family Members
Pilot Study on Random
Samples (15) in nearby locality
KingCircle
Control Group
Pre-test Data Collection*
Diabetes Health Camp on
World Diabetes Day
Experimental Group
Post-test Data Collection done after
subject completes 3 months*
Not given anything Given Herbal Mixture
Ingredients Parts/Ratio Amount per serving/day (g) Total Amount Prepared
for 3 months (g)Morning Night
Amla powder 2 1 1 180
Clove
powder
1 0.5 0.5 90
Hing powder 0.5 0.25 0.25 45
Cinnamon 1.2 0.6 0.6 108
Total 4.7g 423 g
STANDARDIZATION OF HERBAL MIXTURE
Dosage: 1 teaspoon/day (½ – 0 – ½)

5. RESULTS AND DISCUSSIONS
A Herbal Mixture was prepared and administered to diabetic subjects to
check its effectiveness on their insulin resistance.
PS - All the subjects took their medical prescription of OHA-metformin
and only the experimental group was given the Herbal Mixture.

6. Sensory Evaluation of the Herbal Mixture
The texture (3.7/5) of the HM was the most
accepted character against the aroma (2.5),
which was the least accepted by the 15
randomly selected samples.
The texture was followed by appearance
(2.8), mouth feel (2.4) and last the taste
(2.6). All the characters were fairly
accepted and the texture was good.
Since, the powder had a pungent odour
due to strong spices; aroma ranked the
least on a five point scale.
Give the five point scALE ???????
0
0.5
1
1.5
2
2.5
3
3.5
4
Aroma (2.5)
Taste (2.6)
Mouth feel (2.4)
Texture (3.7)
Appearance (2.8)
Overall acceptability
(2.8)
Mean

7.  Background data of your samples to be given in one one line Introduce
our samples Pre test

8.  Give the anthropometric measurement's - the means of wt , ht , bmi
etc pre test

9.  Give the other parameters you have studied like nutrient intakes or food
frequencies of various food groups pre test

10. CONSUMPTION OF PROCESSED/UNPROCESSED OILS
7
5
8
6
10
4
7
6
2
8
2
5
0
2
4
6
8
10
12
Refined Unrefined (kachi gani) Filtered
1 2 3
Processing of Oil
Consumption of processed/unprocessed oils (M & F)
Experimental Male
Control Male
Experimental Females
Control Female

11. DECREASED SLEEP QUALITY
10 10
13
7
5
1010
5
0
2
4
6
8
10
12
14
Yes No
1 2
Sleep Quality decreases past 10 years
Decrease in Quality of sleep in 10 years
(M & F)
Experimental Male
Control Male
Experimental Females
Control Female

12. STRESS LEVELS
2
6
10
2
00
2
15
3
0
2
3
10
0 00
7
5
3
0
0
2
4
6
8
10
12
14
16
1 - 2 point 3 - 4 point 5- 6 point 7 - 8 point 9 - 10 point
1 2 3 4 5
Grade your stress level
Stress levels (M & F)
Experimental Male
Control Male
Experimental Females
Control Female

13.  When the pre and post test results were correlated no significant
differences were observed in the following parameters

14. Pre test and post test RESULTS on anthropometric
1. Anthropometric Indices
Parameter Male Group Pre-test Mean ±
Standard
Deviation
Post-test Mean ±
Standard Deviation
Significance
Level
BMI Experimental 27.7
± 1.83
27
± 1.40
0.027
Control 26.45
± 1.83
25.79
± 1.40
The above table shows that there is an significant effect of the herbal mixture on the BMI of Males

15. Pre and post test results on blood parameters
In the blood parameters there was a significant decrease in the Fasting Blood Sugar
(FBS) (p=0.014) and Haemoglobin A1c (p=0.014) in males is seen from the above
table.
Parameters Male Group Pre-test Mean ±
Standard Deviation
Post-test Mean ±
Standard Deviation
Significance
Level
FBS Experimental 143 ± 5.17 110 ± 5.56 0.014
Control 143 ± 4.75 114 ± 4.33
Hb A1c Experimental 6.63 ± 0.16 5.48 ± 0.19 0.014
Control 6.61 ± 0.16 5.62 ± 0.15
2. Blood Parameters

16. CONCLUSION
On comparing the experimental group with control group (according to
gender) it was found that only the males showed high significance in
the reduction of Fasting Blood Glucose (p=0.014), HbA1c (p=0.014)
and their BMI (p=0.027) while the females didn’t show any
significance. Thus it can be concluded that the herbal mixture did have
some beneficial effect on the diabetic patients