It is planned for guava hybridization-

2. University of Horticultural Sciences
Bagalkot
Submitted by: Priyanka Hugar
Dept. of fruit Science
Course No: GPB 504 (1+1)
Assignment: Hybridization work in Guava
Submitted to: Dr. Satish D
Associate Prof. Plant breeding

3. Introduction:
• Crop: Guava
• B.N.: Psidium guajava L.
• Family: Myrtaceae
• Chromosome number 2n=2x=22
• Origin: Tropical America

4. Cont.
• Important guava growing states in the country are
Uttar Pradesh, Bihar, Madhya Pradesh and
Maharashtra.
• Allahabad district of Uttar Pradesh has the
reputation of growing the best quality of guava
fruits in the world.
• The importance of guava is due to the fact that it is
the hardy fruit which can be grown in alkaline and
poorly drained soil

5. Germplasm resources:
• Guava is mainly a self pollinated crop but occurrence of cross
pollination results in great variation in the seedling population.
• About 103 genotypes are available in the Indian collections
• Yadav (1990) has listed 153 genotypes including Psidium species,
cultivars and hybrids mainly at CISH Lucknow, IIHR Bangalore,
NDUAT Faizabad and HAU Hisar.

6. Floral Biology:
• In guava, flower buds are borne in leaf axil on current season’s
growth, either singly or in cymose of two or three (Braganza, 1990).
• Guava is reported to require about 30 days in Northern India from
flower bud differentiation to complete development upto the calyx
cracking stage (Singh and Sehgal, 1968).
• However, under Bangalore conditions, the period varied from 45 to
51 days (Braganza 1990).

7. Cont.,
• In guava, peak anthesis was found to be between 6 and 7.30 A.M. under
North Indian conditions (Singh and Sehgal, 1968). Dehiscence of anthers
was observed to take place 15 to 30 minutes after anthesis and continued
upto 2hrs (Balasubramanyam, 1959).
• Pollen fertility has been generally found to be high in guava (78 to 91%) in
diploid varieties. Stigmatic receptivity, as studied by fruit set following
controlled pollination, was observed to be maximum on the same day as
anthesis. Stigma was found to be receptive two days before dehiscence,
extending upto 4 days (Singh and Sehgal, 1968).

8. Inheritance pattern:

9. Hybridization technique:
• In guava, flowers that are chosen for crossing are emasculated when
at the ‘calyx break stage’, a day before opening. Pollen from the
pollen parent is brought from an unopened flower, at preferrably at
calyx break stage.
• The stigmatic surface is gently smeared with the pollen and flowers
are bagged.
• Under Bangalore condition pollination carried out during the
morning hours between 10 AM to 12 noon has given better results.

10. Calyx break stage Fruit set after pollination

11. Present programme crosses:
Sardar guava× Navalur guava
Sardar guava × Lalit
Allahabad Safeda × Navalur guava
Allahabad Safeda × Lalit

12. Sardar (Lucknow 49):
• It is a selection from Allahabad Safeda
made at Poona during 1927.
• The plant has a spreading, dwarf with
open, rounded crown.
• The fruits are medium to large and contain
a crisp, soft and creamy pulp; it is a heavy
bearing variety.
• The fruit has a slightly acidic flavour,
attractive aroma, with many seeds, and
good keeping quality.

13. Allahabad Safeda:
• Fruits are round, large in size,
skin with smooth, light yellow on
ripening, pulp white, firm,
excellent in quality with high TSS
and Vitamin C, pleasant flavour
and a few, soft seeds.

14. Navalur guava:
• It is a variety grown in Dharwad district
of Karnataka. It is hardy in nature,
drought tolerant and resistant to canker.
• The important cultivars are: CIW-2
(Channappa Itigatti White), CIW-3, CIW-
4, CIW-5, GR 1 (Ghatage’s Red number
one), GR 3, GW-1 (Ghatage’s white
number), GW-4, SR-1 (Shivammanavar
red number one), SR-2, SW-2
(Shivammanavar white), SWY-1
(Shivammanavar white yalakki).

15. Lalit (CISH -3)
• Selection from half-sib population of
Apple Colour.
• Saffron yellow coloured fruits with
pink flesh , good blend of sugar and
acid. suitable for both table and
processing purposes.
• The pink colour in the beverage
remains stable for more than a year in
storage. 24 % higher yield than
Allahabad Safeda

16. Prediction of the work
• Development of hybrid with soft seeded good quality
and yield.
• Development of hybrid with sardar guava fruit quality
with Lalit guava flesh color.
• Development of hybrid with resistance to biotic and
abiotic stress.

17. References:
 Balasubramanyam, V.R. 1959. Studies on blossom biology of guava (Psidium guajava L.),
Ind. J. Hort.,16:69-75
 Braganza, M.A. 1990. Floral Biology studies and varietal evaluation in genus Psidium.
M.Sc. (Ag). Thesis submitted to University of Agricultural Sciences, Bangalore.
 Dinesh, M. R. and Vasugi C., 2010. Guava improvement in India and future needs. J.
Hortl. Sci. 5:94-108.
 Singh, R. and Sehgal, O.P. 1968. Studies on blossom biology of Psidium guajava L.
(guava). II. Pollen studies, stigma receptivity, pollen and fruit set. Ind. J. Hort., 25:52-59.