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Bio 111 
2009
• Luz se transforma en energía química 
(compuesto orgánico) 
• Ocurre en el cloroplasto 
• Requiere pigmentos fotosintéticos 
• Plantas, algas, bacterias fotosintéticas 
• CO2 se convierte en carbohidrato
luz 
6 CO2 + 12H2O ---> C6H12O6 +6O2 +6H2O 
clorofila
(chloroplast) 
photosynthesis 
CO ATP sugar O2 2 H2O 
cellular 
respiration 
(mitochondrion) 
Figure 7-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
Luz 
◦ Compuesta de fotones 
 Luz visible es una porción 
pequeña del espectro 
electromagnético 
◦ La energía viaja en ondas 
◦ Onda corta más energía que onda 
larga
 Fotosíntesis es un proceso 
redox 
◦ Se captura la energía solar y se 
transforma en carbohidrato 
◦ Hidrógenos del agua reducen el 
carbono 
◦ Oxígeno del agua se oxida
TV and 
radio 
waves 
Micro-waves 
Infrared 
Visible 
UV 
X-rays 
Gamma 
rays 
Color 
spectrum 
of visible 
light 
One wavelength 
Longer wavelength 
Red 
Orange 
Yellow 
Green 
Blue 
Violet 
760 nm 
700 nm 
600 nm 
500 nm 
400 nm 
380 nm 
Electromagnetic 
spectrum Shorter wavelength
100μm 
(a) 
Wavelength of light (nm) 
(b)
 Cloroplastos 
◦ Organelos rodeados de doble 
membrana 
◦ Lugar de fotosíntesis 
◦ Localizados principalmente en el 
mesófilo de la hoja
Palisade 
mesophyll 
Vein 
Spongy mesophyll 
(a) Stoma
Chloroplast 
outer membrane 
inner membrane 
thylakoid 
stroma 
channel 
interconnecting 
thylakoids 
Figure 7-2d Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
(b) 10μm 
Thylakoids Outer membrane 
Inner 
membrane 
Intermembrane 
space 
Thylakoid 
membrane 
Stroma Granum 
(stack of 
thylakoids) 
Thylakoid 
(c) lumen
 Clorofila 
◦ Pigmento fotosintético -principal 
◦ Clorofila a, clorofila b, carotenoides, 
se encuentran en las membranas 
tilacoides de los cloroplastos
 Fotosistemas I y II 
◦ Dos tipos de unidades fotosintéticas 
◦ Cada fotosistema incluye 
 Moléculas de clorofila 
 Complejos de antenas 
◦ Centro de reacción del Fotosistema I 
 P700 pico de absorción en 700 nm 
◦ Centro de reacción Fotosistema II 
 P680 pico de reabsorción en 680 nm
Primary 
electron 
acceptor 
Photon 
Thylakoid 
Chloroplast 
Photosystem 
Antenna 
complexes 
Reaction 
center 
e–
Estimated absorption (%) 
Chlorophyll b 
Chlorophyll a 
Wavelength (nm) 
(a) 
Relative rate of photosynthesis 
Wavelength (nm) 
(b)
chlorophyll b 
carotenoids 
Wavelength (nanometers) 
Micro-waves 
Gamma rays X-rays UV Infrared 
Figure 7-5 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 
Radio 
waves 
Visible light 
higher energy 
(too much) 
lower energy 
(not enough) 
light absorption (percent) 
Absorbance of photosynthetic pigments 
chlorophyll a
 Fases de fotosíntesis 
◦ Dependiente de luz 
 tilacoides 
 Los electrones energizados por la luz se 
convierten en ATP 
◦ Fijación de carbono 
 estroma 
 Compuestos productos de la fase 
dependiente se requieren para la 
formación de carbohidrato
H2O 
DEPLETED 
CARRIERS 
(ADP, NADP+) 
CO2 
LIGHT-DEPENDENT 
REACTIONS 
(in thylakoids) 
O2 
ENERGIZED 
CARRIERS 
(ATP, NADPH) 
LIGHT-INDEPENDENT 
REACTIONS 
(in stroma) 
Figure 7-4 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 
glucose
O2 
energy from 
sunlight 
ATP 
NADPH 
Light-dependent 
reactions are 
associated with 
thylakoids. 
chloroplast 
CO2 
Light-independent 
reactions 
(C3 cycle) occur 
in stroma. 
ADP 
NADP+ 
sugar 
H2O 
Figure 7-11 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
 La luz es absorbida por la clorofila 
 Clorofila excitada-nivel energético alto 
 Libera electrones altos en energía, se ioniza, 
queda con carga positiva-Fotoionización 
 Electrones pasan cadena transporte 
 Se degradan energéticamente 
 cíclica 
 no cíclica 
 La clorofila se neutraliza
 Transporte de electrones 
cíclica 
◦ Electrones del fotosistema I regresan 
al fotosistema I 
◦ Se genera ATP por quimiosmosis 
◦ No NADPH, no O2
Light-dependent reactions Carbon fixation reactions 
ATP 
ADP 
NADPH 
NADP+ 
Light 
reactions 
Calvin 
cycle 
H2O O2 
CO2 
Chloroplast 
Carbohydrates
Light-dependent reactions 
(in thylakoids) 
Carbon fixation reactions 
(in stroma) 
ATP 
ADP 
NADPH 
NADP+ 
Light 
reactions 
Calvin 
cycle 
H2O O2 CO2 
Chloroplast 
Carbohydrates
 Transporte de electrones no 
cíclica 
◦ Se forma ATP y NADPH 
◦ Electrones energizados por luz son 
aceptados por NADP+ 
◦ Una serie de reacciones redox 
◦ Electrones que provienen de P680 
son reemplazados por los que 
provienen del H2O 
◦ Ocurre fotolisis
H2O -- ½ O2 + 2H+ 2e_
Oxidation- reduction potential (volts) (relative energy level) 
Primary 
electron 
acceptor 
Primary 
electron 
acceptor 
NADPH 
NADP+ 
ATP 
H2O 
O2 
ADP 
1 
2 
Photosystem II(P680) 
Production 
of ATP by 
chemiosmosis 
H+ 
Ferredoxin 
Plastiquinone 
Cytochrome 
complex 
Plastocyanin 
1/2 + 2 H+ 
Pi 
A0 
A1 
FeSx 
FeSB 
FeSA 
(from medium) 
Photosystem I(P700) 
Electron 
transport 
chain 
Electron 
transport 
chain 
2e- 
2 e
ETC 
Energy from energized 
electrons powers active 
transport of H+ by ETC. 
Figure 7-8 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 
C3 
cycle 
PSII PSI 
stroma 
ETC 
thylakoid space 
Energy from 
energized 
electrons powers 
NADPH synthesis. 
Flow of H+ down 
concentration gradient 
powers ATP synthesis. 
High H+ concentration 
generated by active 
transport. 
H+ channel coupled 
to ATP-synthesizing 
enzyme. 
Energy-carrier 
molecules power 
the C3 cycle.
 Síntesis de ATP y transporte de 
electrones 
◦ Electrones se mueven en cadena de 
electrones 
◦ Protones (H+) se mueven de la 
estroma hacia el lumen del tilacoide, 
creando un gradiente de protones
stroma 
Figure E7-2 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 
thylakoid 
membrane 
photosystem II 
2e– 
ADP ATP 
P 
Active transport 
of hydrogen ions. 
High H+ concentration 
in thylakoid space. 
H+ ion channel coupled 
to ATP synthesizing 
enzyme. 
Flow of H+ powers 
ATP synthesis.
 ATP se utilizan en estroma 
 NADPH se utiliza en estroma 
 O2 se libera al aire
Stroma 
Thylakoid lumen 
Thylakoid membrane Photons (H+)
Light-dependent reactions Carbon fixation reactions 
ATP 
ADP 
NADPH 
NADP+ 
Light 
reactions 
Calvin 
cycle 
H2O O2 
CO2 
Chloroplast 
Carbohydrates
 Fijación del Carbono 
◦ Se forma carbohidrato a partir de 
CO2 , ATP, y NADPH 
12 NADPH + 18 ATP + 6 CO2 → 
C6H12O6 + 12 NADP + + 18 ADP + 18 Pi + 6 H2O
 Fases del ciclo de Calvin 
◦ capturar CO2 
◦ reducción del carbono 
◦ regeneración de RuBP
PGA 
G3P 
Figure 7-12a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 
CO2 
C3 plants use the C3 pathway 
bundle-sheath 
cells 
In a C3 plant, mesophyll cells 
contain chloroplasts; bundle-sheath 
cells do not. 
Much photorespiration 
occurs under hot, dry 
conditions. 
CO2 
rubisco 
Little glucose 
is synthesized. 
O2 
RuBP 
glucose 
within mesophyll chloropast 
stoma 
C3 
Cycle
CO 2 molecules are 
captured by RuBP, 
resulting in an unstable 
intermediate that is 
immediately broken 
apart into 2 PGA 
12 molecules of 
phosphoglycerate 
(PGA) 
12 ATP 
12 ADP 
PGA is phosphorylated 
by ATP and reduced by 
NADPH. Removal of a 
phosphate results in 
formation of G3P. 
1 
Through a series of 
reactions G3P is 
rearranged into new 
RuBP molecules or 
another sugar 
6 ADP 
ATP 
P P 
6 molecules of ribulose 
phosphate (RP) 
Glucose and other 
carbohydrate synthesis 
6 molecules of 
ribulose bisphosphate 
(RuBP) 
2 molecules 
of glyceraldehyde-3- 
phosphate (G3P) 
CALVIN 
CYCLE 
Carbon 
reduction 
phase 
RuBP 
regeneration 
phase 
CO2 uptake 
phase 
12 NADPH 
6 molecules of CO2 
10 molecules 
of G3P 
P 
P 
P 
P 
P 
P 
12 molecules 
of glyceraldehyde-3- 
phosphate (G3P) 
3 
2 
12 NADP+
Fotosíntesis` final2009

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Fotosíntesis` final2009

  • 2. • Luz se transforma en energía química (compuesto orgánico) • Ocurre en el cloroplasto • Requiere pigmentos fotosintéticos • Plantas, algas, bacterias fotosintéticas • CO2 se convierte en carbohidrato
  • 3. luz 6 CO2 + 12H2O ---> C6H12O6 +6O2 +6H2O clorofila
  • 4. (chloroplast) photosynthesis CO ATP sugar O2 2 H2O cellular respiration (mitochondrion) Figure 7-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
  • 5. Luz ◦ Compuesta de fotones  Luz visible es una porción pequeña del espectro electromagnético ◦ La energía viaja en ondas ◦ Onda corta más energía que onda larga
  • 6.  Fotosíntesis es un proceso redox ◦ Se captura la energía solar y se transforma en carbohidrato ◦ Hidrógenos del agua reducen el carbono ◦ Oxígeno del agua se oxida
  • 7. TV and radio waves Micro-waves Infrared Visible UV X-rays Gamma rays Color spectrum of visible light One wavelength Longer wavelength Red Orange Yellow Green Blue Violet 760 nm 700 nm 600 nm 500 nm 400 nm 380 nm Electromagnetic spectrum Shorter wavelength
  • 8. 100μm (a) Wavelength of light (nm) (b)
  • 9.  Cloroplastos ◦ Organelos rodeados de doble membrana ◦ Lugar de fotosíntesis ◦ Localizados principalmente en el mesófilo de la hoja
  • 10. Palisade mesophyll Vein Spongy mesophyll (a) Stoma
  • 11. Chloroplast outer membrane inner membrane thylakoid stroma channel interconnecting thylakoids Figure 7-2d Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
  • 12. (b) 10μm Thylakoids Outer membrane Inner membrane Intermembrane space Thylakoid membrane Stroma Granum (stack of thylakoids) Thylakoid (c) lumen
  • 13.  Clorofila ◦ Pigmento fotosintético -principal ◦ Clorofila a, clorofila b, carotenoides, se encuentran en las membranas tilacoides de los cloroplastos
  • 14.  Fotosistemas I y II ◦ Dos tipos de unidades fotosintéticas ◦ Cada fotosistema incluye  Moléculas de clorofila  Complejos de antenas ◦ Centro de reacción del Fotosistema I  P700 pico de absorción en 700 nm ◦ Centro de reacción Fotosistema II  P680 pico de reabsorción en 680 nm
  • 15. Primary electron acceptor Photon Thylakoid Chloroplast Photosystem Antenna complexes Reaction center e–
  • 16. Estimated absorption (%) Chlorophyll b Chlorophyll a Wavelength (nm) (a) Relative rate of photosynthesis Wavelength (nm) (b)
  • 17. chlorophyll b carotenoids Wavelength (nanometers) Micro-waves Gamma rays X-rays UV Infrared Figure 7-5 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Radio waves Visible light higher energy (too much) lower energy (not enough) light absorption (percent) Absorbance of photosynthetic pigments chlorophyll a
  • 18.  Fases de fotosíntesis ◦ Dependiente de luz  tilacoides  Los electrones energizados por la luz se convierten en ATP ◦ Fijación de carbono  estroma  Compuestos productos de la fase dependiente se requieren para la formación de carbohidrato
  • 19. H2O DEPLETED CARRIERS (ADP, NADP+) CO2 LIGHT-DEPENDENT REACTIONS (in thylakoids) O2 ENERGIZED CARRIERS (ATP, NADPH) LIGHT-INDEPENDENT REACTIONS (in stroma) Figure 7-4 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. glucose
  • 20. O2 energy from sunlight ATP NADPH Light-dependent reactions are associated with thylakoids. chloroplast CO2 Light-independent reactions (C3 cycle) occur in stroma. ADP NADP+ sugar H2O Figure 7-11 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
  • 21.  La luz es absorbida por la clorofila  Clorofila excitada-nivel energético alto  Libera electrones altos en energía, se ioniza, queda con carga positiva-Fotoionización  Electrones pasan cadena transporte  Se degradan energéticamente  cíclica  no cíclica  La clorofila se neutraliza
  • 22.  Transporte de electrones cíclica ◦ Electrones del fotosistema I regresan al fotosistema I ◦ Se genera ATP por quimiosmosis ◦ No NADPH, no O2
  • 23. Light-dependent reactions Carbon fixation reactions ATP ADP NADPH NADP+ Light reactions Calvin cycle H2O O2 CO2 Chloroplast Carbohydrates
  • 24. Light-dependent reactions (in thylakoids) Carbon fixation reactions (in stroma) ATP ADP NADPH NADP+ Light reactions Calvin cycle H2O O2 CO2 Chloroplast Carbohydrates
  • 25.  Transporte de electrones no cíclica ◦ Se forma ATP y NADPH ◦ Electrones energizados por luz son aceptados por NADP+ ◦ Una serie de reacciones redox ◦ Electrones que provienen de P680 son reemplazados por los que provienen del H2O ◦ Ocurre fotolisis
  • 26. H2O -- ½ O2 + 2H+ 2e_
  • 27. Oxidation- reduction potential (volts) (relative energy level) Primary electron acceptor Primary electron acceptor NADPH NADP+ ATP H2O O2 ADP 1 2 Photosystem II(P680) Production of ATP by chemiosmosis H+ Ferredoxin Plastiquinone Cytochrome complex Plastocyanin 1/2 + 2 H+ Pi A0 A1 FeSx FeSB FeSA (from medium) Photosystem I(P700) Electron transport chain Electron transport chain 2e- 2 e
  • 28. ETC Energy from energized electrons powers active transport of H+ by ETC. Figure 7-8 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. C3 cycle PSII PSI stroma ETC thylakoid space Energy from energized electrons powers NADPH synthesis. Flow of H+ down concentration gradient powers ATP synthesis. High H+ concentration generated by active transport. H+ channel coupled to ATP-synthesizing enzyme. Energy-carrier molecules power the C3 cycle.
  • 29.  Síntesis de ATP y transporte de electrones ◦ Electrones se mueven en cadena de electrones ◦ Protones (H+) se mueven de la estroma hacia el lumen del tilacoide, creando un gradiente de protones
  • 30. stroma Figure E7-2 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. thylakoid membrane photosystem II 2e– ADP ATP P Active transport of hydrogen ions. High H+ concentration in thylakoid space. H+ ion channel coupled to ATP synthesizing enzyme. Flow of H+ powers ATP synthesis.
  • 31.  ATP se utilizan en estroma  NADPH se utiliza en estroma  O2 se libera al aire
  • 32.
  • 33. Stroma Thylakoid lumen Thylakoid membrane Photons (H+)
  • 34. Light-dependent reactions Carbon fixation reactions ATP ADP NADPH NADP+ Light reactions Calvin cycle H2O O2 CO2 Chloroplast Carbohydrates
  • 35.  Fijación del Carbono ◦ Se forma carbohidrato a partir de CO2 , ATP, y NADPH 12 NADPH + 18 ATP + 6 CO2 → C6H12O6 + 12 NADP + + 18 ADP + 18 Pi + 6 H2O
  • 36.  Fases del ciclo de Calvin ◦ capturar CO2 ◦ reducción del carbono ◦ regeneración de RuBP
  • 37. PGA G3P Figure 7-12a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. CO2 C3 plants use the C3 pathway bundle-sheath cells In a C3 plant, mesophyll cells contain chloroplasts; bundle-sheath cells do not. Much photorespiration occurs under hot, dry conditions. CO2 rubisco Little glucose is synthesized. O2 RuBP glucose within mesophyll chloropast stoma C3 Cycle
  • 38. CO 2 molecules are captured by RuBP, resulting in an unstable intermediate that is immediately broken apart into 2 PGA 12 molecules of phosphoglycerate (PGA) 12 ATP 12 ADP PGA is phosphorylated by ATP and reduced by NADPH. Removal of a phosphate results in formation of G3P. 1 Through a series of reactions G3P is rearranged into new RuBP molecules or another sugar 6 ADP ATP P P 6 molecules of ribulose phosphate (RP) Glucose and other carbohydrate synthesis 6 molecules of ribulose bisphosphate (RuBP) 2 molecules of glyceraldehyde-3- phosphate (G3P) CALVIN CYCLE Carbon reduction phase RuBP regeneration phase CO2 uptake phase 12 NADPH 6 molecules of CO2 10 molecules of G3P P P P P P P 12 molecules of glyceraldehyde-3- phosphate (G3P) 3 2 12 NADP+