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Bio 111
2009
• Luz se transforma en energía química
(compuesto orgánico)
• Ocurre en el cloroplasto
• Requiere pigmentos fotosintéticos
• Plantas, algas, bacterias fotosintéticas
• CO2 se convierte en carbohidrato
luz
6 CO2 + 12H2O ---> C6H12O6 +6O2 +6H2O
clorofila
Figure 7-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
photosynthesis
cellular
respiration
(mitochondrion)
O2ATP sugarCO2H2O
(chloroplast)
 Luz
◦ Compuesta de fotones
 Luz visible es una porción
pequeña del espectro
electromagnético
◦ La energía viaja en ondas
◦ Onda corta más energía que onda
larga
 Fotosíntesis es un proceso
redox
◦ Se captura la energía solar y se
transforma en carbohidrato
◦ Hidrógenos del agua reducen el
carbono
◦ Oxígeno del agua se oxida
TV and
radio
waves
Micro-
waves
Infrared
Visible
UV
X-rays
Gamma
rays
Color
spectrum
of visible
light
Red
Orange
Yellow
Green
Blue
Violet
760 nm
700 nm
600 nm
500 nm
400 nm
380 nm
One wavelength
Longer wavelength
Electromagnetic
spectrum Shorter wavelength
100µm
(a)
Wavelength of light (nm)
(b)
 Cloroplastos
◦ Organelos rodeados de doble
membrana
◦ Lugar de fotosíntesis
◦ Localizados principalmente en el
mesófilo de la hoja
Palisade
mesophyll
Vein
Spongy mesophyll
Stoma(a)
Figure 7-2d Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
Chloroplast
channel
interconnecting
thylakoids
stroma
thylakoid
outer membrane
inner membrane
10µm(b)
Thylakoids Outer membrane
Inner
membrane
Intermembrane
space
Thylakoid
membrane
Stroma Granum
(stack of
thylakoids)
Thylakoid
lumen(c)
 Clorofila
◦ Pigmento fotosintético -principal
◦ Clorofila a, clorofila b, carotenoides,
se encuentran en las membranas
tilacoides de los cloroplastos
 Fotosistemas I y II
◦ Dos tipos de unidades fotosintéticas
◦ Cada fotosistema incluye
 Moléculas de clorofila
 Complejos de antenas
◦ Centro de reacción del Fotosistema I
 P700 pico de absorción en 700 nm
◦ Centro de reacción Fotosistema II
 P680 pico de reabsorción en 680 nm
Primary
electron
acceptor
Photon
Photosystem
Chloroplast
Thylakoid
Antenna
complexes
Reaction
center
e–
Estimatedabsorption(%)
Wavelength (nm)
Chlorophyll b
Chlorophyll a
(a)
Relativerateofphotosynthesis
Wavelength (nm)
(b)
Figure 7-5 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
chlorophyll b
Wavelength (nanometers)
Micro-
waves
Gamma rays X-rays UV Infrared
Radio
waves
Visible light
higher energy
(too much)
lower energy
(not enough)
lightabsorption(percent)
Absorbance of photosynthetic pigments
chlorophyll a
carotenoids
 Fases de fotosíntesis
◦ Dependiente de luz
 tilacoides
 Los electrones energizados por la luz se
convierten en ATP
◦ Fijación de carbono
 estroma
 Compuestos productos de la fase
dependiente se requieren para la
formación de carbohidrato
Figure 7-4 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
CO2
LIGHT-DEPENDENT
REACTIONS
(in thylakoids)
LIGHT-INDEPENDENT
REACTIONS
(in stroma)
DEPLETED
CARRIERS
(ADP, NADP+)
ENERGIZED
CARRIERS
(ATP, NADPH)
H2O
glucose
O2
Figure 7-11 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
O2
energy from
sunlight
chloroplast
Light-
independent
reactions
(C3 cycle) occur
in stroma.
Light-dependent
reactions are
associated with
thylakoids.
CO2
NADP+
sugar
NADPH
ADP
ATP
H2O
 La luz es absorbida por la clorofila
 Clorofila excitada-nivel energético alto
 Libera electrones altos en energía, se ioniza,
queda con carga positiva-Fotoionización
 Electrones pasan cadena transporte
 Se degradan energéticamente
 cíclica
 no cíclica
 La clorofila se neutraliza
 Transporte de electrones
cíclica
◦ Electrones del fotosistema I regresan
al fotosistema I
◦ Se genera ATP por quimiosmosis
◦ No NADPH, no O2
Light-dependent reactions Carbon fixation reactions
ATP
ADP
NADPH
NADP+
Light
reactions
Calvin
cycle
H2O O2
CO2
Carbohydrates
Chloroplast
Light-dependent reactions
(in thylakoids)
Carbon fixation reactions
(in stroma)
ATP
ADP
NADPH
NADP+
Light
reactions
Calvin
cycle
H2O O2
CO2
Carbohydrates
Chloroplast
 Transporte de electrones no
cíclica
◦ Se forma ATP y NADPH
◦ Electrones energizados por luz son
aceptados por NADP+
◦ Una serie de reacciones redox
◦ Electrones que provienen de P680
son reemplazados por los que
provienen del H2O
◦ Ocurre fotolisis
H2O -- ½ O2 + 2H+ 2e_
Oxidation-reductionpotential(volts)(relativeenergylevel)
Primary
electron
acceptor
Primary
electron
acceptor
NADPH
NADP+
H2O
ATP
O2
ADP
1
2
Photosystem II(P680)
Production
of ATP by
chemiosmosis
H+
Ferredoxin
Plastiquinone
Cytochrome
complex
Plastocyanin
1/2 + 2 H+
Pi
A0
A1
FeSx
FeSB
FeSA
Photosystem I(P700)
(from medium)
Electron
transport
chain
Electron
transport
chain
2e-
2 e
Figure 7-8 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
C3
cycle
PSII PSI
ETC
stroma
ETC
thylakoid space
Energy from
energized
electrons powers
NADPH synthesis.
Flow of H+ down
concentration gradient
powers ATP synthesis.
Energy from energized
electrons powers active
transport of H+ by ETC.
High H+ concentration
generated by active
transport.
H+ channel coupled
to ATP-synthesizing
enzyme.
Energy-carrier
molecules power
the C3 cycle.
 Síntesis de ATP y transporte de
electrones
◦ Electrones se mueven en cadena de
electrones
◦ Protones (H+) se mueven de la
estroma hacia el lumen del tilacoide,
creando un gradiente de protones
Figure E7-2 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
stroma
thylakoid
membrane
photosystem II
2e–
ATPADP
P
Active transport
of hydrogen ions.
High H+ concentration
in thylakoid space.
H+ ion channel coupled
to ATP synthesizing
enzyme.
Flow of H+ powers
ATP synthesis.
 ATP se utilizan en estroma
 NADPH se utiliza en estroma
 O2 se libera al aire
Stroma
Thylakoid lumen
Thylakoid membrane Photons (H+)
Light-dependent reactions Carbon fixation reactions
ATP
ADP
NADPH
NADP+
Light
reactions
Calvin
cycle
H2O O2
CO2
Carbohydrates
Chloroplast
 Fijación del Carbono
◦ Se forma carbohidrato a partir de
CO2 , ATP, y NADPH
12 NADPH + 18 ATP + 6 CO2 →
C6H12O6 + 12 NADP + + 18 ADP + 18 Pi + 6 H2O
 Fases del ciclo de Calvin
◦ capturar CO2
◦ reducción del carbono
◦ regeneración de RuBP
Figure 7-12a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.
CO2
C3 plants use the C3 pathway
bundle-
sheath
cells
In a C3 plant, mesophyll cells
contain chloroplasts; bundle-
sheath cells do not.
Much photorespiration
occurs under hot, dry
conditions.
Little glucose
is synthesized.
rubisco
G3P
CO2
PGA
O2
RuBP
glucose
within mesophyll chloropast
stoma
C3
Cycle
CO 2 molecules are
captured by RuBP,
resulting in an unstable
intermediate that is
immediately broken
apart into 2 PGA
PGA is phosphorylated
by ATP and reduced by
NADPH. Removal of a
phosphate results in
formation of G3P.
Through a series of
reactions G3P is
rearranged into new
RuBP molecules or
another sugar
Glucose and other
carbohydrate synthesis
2 molecules
of glyceraldehyde-3-
phosphate (G3P)
6 molecules of
ribulose bisphosphate
(RuBP)
CALVIN
CYCLE
Carbon
reduction
phase
RuBP
regeneration
phase
CO2 uptake
phase
12 NADPH
12 ADP
12 molecules of
phosphoglycerate
(PGA)
12 ATP
6 molecules of CO2
10 molecules
of G3P
6 molecules of ribulose
phosphate (RP)
ATP
6 ADP
P
P
P
P
P
P P
P
12 molecules
of glyceraldehyde-3-
phosphate (G3P)
1
3
2
12 NADP+
Fotosíntesis` final2009

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Fotosíntesis` final2009

  • 2. • Luz se transforma en energía química (compuesto orgánico) • Ocurre en el cloroplasto • Requiere pigmentos fotosintéticos • Plantas, algas, bacterias fotosintéticas • CO2 se convierte en carbohidrato
  • 3. luz 6 CO2 + 12H2O ---> C6H12O6 +6O2 +6H2O clorofila
  • 4. Figure 7-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. photosynthesis cellular respiration (mitochondrion) O2ATP sugarCO2H2O (chloroplast)
  • 5.  Luz ◦ Compuesta de fotones  Luz visible es una porción pequeña del espectro electromagnético ◦ La energía viaja en ondas ◦ Onda corta más energía que onda larga
  • 6.  Fotosíntesis es un proceso redox ◦ Se captura la energía solar y se transforma en carbohidrato ◦ Hidrógenos del agua reducen el carbono ◦ Oxígeno del agua se oxida
  • 7. TV and radio waves Micro- waves Infrared Visible UV X-rays Gamma rays Color spectrum of visible light Red Orange Yellow Green Blue Violet 760 nm 700 nm 600 nm 500 nm 400 nm 380 nm One wavelength Longer wavelength Electromagnetic spectrum Shorter wavelength
  • 9.  Cloroplastos ◦ Organelos rodeados de doble membrana ◦ Lugar de fotosíntesis ◦ Localizados principalmente en el mesófilo de la hoja
  • 11. Figure 7-2d Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Chloroplast channel interconnecting thylakoids stroma thylakoid outer membrane inner membrane
  • 13.  Clorofila ◦ Pigmento fotosintético -principal ◦ Clorofila a, clorofila b, carotenoides, se encuentran en las membranas tilacoides de los cloroplastos
  • 14.  Fotosistemas I y II ◦ Dos tipos de unidades fotosintéticas ◦ Cada fotosistema incluye  Moléculas de clorofila  Complejos de antenas ◦ Centro de reacción del Fotosistema I  P700 pico de absorción en 700 nm ◦ Centro de reacción Fotosistema II  P680 pico de reabsorción en 680 nm
  • 16. Estimatedabsorption(%) Wavelength (nm) Chlorophyll b Chlorophyll a (a) Relativerateofphotosynthesis Wavelength (nm) (b)
  • 17. Figure 7-5 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. chlorophyll b Wavelength (nanometers) Micro- waves Gamma rays X-rays UV Infrared Radio waves Visible light higher energy (too much) lower energy (not enough) lightabsorption(percent) Absorbance of photosynthetic pigments chlorophyll a carotenoids
  • 18.  Fases de fotosíntesis ◦ Dependiente de luz  tilacoides  Los electrones energizados por la luz se convierten en ATP ◦ Fijación de carbono  estroma  Compuestos productos de la fase dependiente se requieren para la formación de carbohidrato
  • 19. Figure 7-4 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. CO2 LIGHT-DEPENDENT REACTIONS (in thylakoids) LIGHT-INDEPENDENT REACTIONS (in stroma) DEPLETED CARRIERS (ADP, NADP+) ENERGIZED CARRIERS (ATP, NADPH) H2O glucose O2
  • 20. Figure 7-11 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. O2 energy from sunlight chloroplast Light- independent reactions (C3 cycle) occur in stroma. Light-dependent reactions are associated with thylakoids. CO2 NADP+ sugar NADPH ADP ATP H2O
  • 21.  La luz es absorbida por la clorofila  Clorofila excitada-nivel energético alto  Libera electrones altos en energía, se ioniza, queda con carga positiva-Fotoionización  Electrones pasan cadena transporte  Se degradan energéticamente  cíclica  no cíclica  La clorofila se neutraliza
  • 22.  Transporte de electrones cíclica ◦ Electrones del fotosistema I regresan al fotosistema I ◦ Se genera ATP por quimiosmosis ◦ No NADPH, no O2
  • 23. Light-dependent reactions Carbon fixation reactions ATP ADP NADPH NADP+ Light reactions Calvin cycle H2O O2 CO2 Carbohydrates Chloroplast
  • 24. Light-dependent reactions (in thylakoids) Carbon fixation reactions (in stroma) ATP ADP NADPH NADP+ Light reactions Calvin cycle H2O O2 CO2 Carbohydrates Chloroplast
  • 25.  Transporte de electrones no cíclica ◦ Se forma ATP y NADPH ◦ Electrones energizados por luz son aceptados por NADP+ ◦ Una serie de reacciones redox ◦ Electrones que provienen de P680 son reemplazados por los que provienen del H2O ◦ Ocurre fotolisis
  • 26. H2O -- ½ O2 + 2H+ 2e_
  • 27. Oxidation-reductionpotential(volts)(relativeenergylevel) Primary electron acceptor Primary electron acceptor NADPH NADP+ H2O ATP O2 ADP 1 2 Photosystem II(P680) Production of ATP by chemiosmosis H+ Ferredoxin Plastiquinone Cytochrome complex Plastocyanin 1/2 + 2 H+ Pi A0 A1 FeSx FeSB FeSA Photosystem I(P700) (from medium) Electron transport chain Electron transport chain 2e- 2 e
  • 28. Figure 7-8 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. C3 cycle PSII PSI ETC stroma ETC thylakoid space Energy from energized electrons powers NADPH synthesis. Flow of H+ down concentration gradient powers ATP synthesis. Energy from energized electrons powers active transport of H+ by ETC. High H+ concentration generated by active transport. H+ channel coupled to ATP-synthesizing enzyme. Energy-carrier molecules power the C3 cycle.
  • 29.  Síntesis de ATP y transporte de electrones ◦ Electrones se mueven en cadena de electrones ◦ Protones (H+) se mueven de la estroma hacia el lumen del tilacoide, creando un gradiente de protones
  • 30. Figure E7-2 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. stroma thylakoid membrane photosystem II 2e– ATPADP P Active transport of hydrogen ions. High H+ concentration in thylakoid space. H+ ion channel coupled to ATP synthesizing enzyme. Flow of H+ powers ATP synthesis.
  • 31.  ATP se utilizan en estroma  NADPH se utiliza en estroma  O2 se libera al aire
  • 32.
  • 34. Light-dependent reactions Carbon fixation reactions ATP ADP NADPH NADP+ Light reactions Calvin cycle H2O O2 CO2 Carbohydrates Chloroplast
  • 35.  Fijación del Carbono ◦ Se forma carbohidrato a partir de CO2 , ATP, y NADPH 12 NADPH + 18 ATP + 6 CO2 → C6H12O6 + 12 NADP + + 18 ADP + 18 Pi + 6 H2O
  • 36.  Fases del ciclo de Calvin ◦ capturar CO2 ◦ reducción del carbono ◦ regeneración de RuBP
  • 37. Figure 7-12a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. CO2 C3 plants use the C3 pathway bundle- sheath cells In a C3 plant, mesophyll cells contain chloroplasts; bundle- sheath cells do not. Much photorespiration occurs under hot, dry conditions. Little glucose is synthesized. rubisco G3P CO2 PGA O2 RuBP glucose within mesophyll chloropast stoma C3 Cycle
  • 38. CO 2 molecules are captured by RuBP, resulting in an unstable intermediate that is immediately broken apart into 2 PGA PGA is phosphorylated by ATP and reduced by NADPH. Removal of a phosphate results in formation of G3P. Through a series of reactions G3P is rearranged into new RuBP molecules or another sugar Glucose and other carbohydrate synthesis 2 molecules of glyceraldehyde-3- phosphate (G3P) 6 molecules of ribulose bisphosphate (RuBP) CALVIN CYCLE Carbon reduction phase RuBP regeneration phase CO2 uptake phase 12 NADPH 12 ADP 12 molecules of phosphoglycerate (PGA) 12 ATP 6 molecules of CO2 10 molecules of G3P 6 molecules of ribulose phosphate (RP) ATP 6 ADP P P P P P P P P 12 molecules of glyceraldehyde-3- phosphate (G3P) 1 3 2 12 NADP+