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Salmonella have ability to penetrate tissue and can specifically target tumor cells. Genetically modified strain of Salmonella delivers a protein which induces the apoptosis in tumor cells and finally results into the activation of immune system which leads to the suppression and eradication of the tumor from the host.
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2. Tumor antigens are classified based on their expression patterns and molecular structure. They include tumor specific antigens from viruses, overexpressed cellular proteins, products of mutated oncogenes, oncofetal antigens, and antigens from altered glycoproteins.
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Berta CasarConsorci Universitat Internacional Menéndez Pelayo Barcelona (CUIMPB) - Centre Ernest Lluch
The document summarizes research using chick embryo models to study cancer metastasis. It describes several advantages of the chick embryo model, including its ability to support rapid growth of xenografted human tumor cells. Several chick embryo cancer models are outlined, including models for spontaneous metastasis, analyzing tumor cell interactions with vasculature, and studying invasion and extravasation. Examples are given of studies using these models to analyze tumor growth and metastasis of various cancer cell lines and effects of potential therapeutics.publi Toulouse
This document describes a study that conducted an RNA interference screening in breast cancer cells to identify epigenetic factors regulating the mesenchyme to epithelium transition (MET). Researchers designed a siRNA library targeting 729 chromatin modification genes and screened it in the mesenchymal breast cancer cell line MDA-MB-231. They identified 70 candidate genes involved in MET, including known genes like ZEB1, G9a, SMAD5 and SMARCD3, as well as DOT1L which has been implicated in MET. They also identified KAT5 as a novel gene linked to maintaining the mesenchymal phenotype for the first time. The screening approach involved measuring E-cadherin induction and cell
Mcpyv Presentation
This document discusses the role of Merkel cell polyomavirus (MCPyV) in the skin cancer Merkel cell carcinoma (MCC). It outlines three aims: 1) to determine if MCPyV can transform human cells and cause tumors in animals, 2) to identify wild-type MCPyV and test its infectivity and lytic potential, and 3) to investigate the mechanism of MCPyV-induced transformation through viral protein expression and interactions with cellular proteins. The identification of MCPyV in the majority of MCC tumors suggests it may be the first known human polyomavirus to cause cancer, which could lead to new treatment strategies if the virus's role is fully characterized.
Mi r 449b inhibits the migration and invasion of colorectal cancer cells thro...
Mi r 449b inhibits the migration and invasion of colorectal cancer cells thro...Clinical Surgery Research Communications
- miR-449b expression was significantly decreased in human CRC tissues and cell lines compared to normal tissues, and was even lower in metastatic CRC tissues.
- Forced expression of miR-449b in CRC cells significantly inhibited cell migration and invasion in vitro.
- miR-449b was found to negatively regulate MMP2 in CRC cells, and overexpression of MMP2 reversed the inhibitory effects of miR-449b on cell invasion and migration.Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Mohamed Khalid Ali Xundhur
The document summarizes a study that examined gene expression profiling in MCF-7 breast cancer cells infected with Newcastle disease virus (NDV). The study found that NDV infection changed the expression levels of many genes involved in tumor progression, cell cycle regulation, apoptosis, and other cellular processes. Specifically, the study used a gene expression kit to measure changes in 21 genes related to these processes in MCF-7 cells infected with NDV. It found that NDV infection altered the expression of genes like PUMA, Bcl-2, ESR1, and MYBL2. The results provide insight into the gene regulation mechanisms by which NDV selectively kills cancer cells.Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Mohamed Khalid Ali Xundhur
1) The document analyzes gene expression profiling in MCF-7 breast cancer cells infected with Newcastle disease virus (NDV). It finds that NDV infection changes the expression level of many genes involved in tumor progression, cell cycle regulation, apoptosis, and other processes.
2) Specifically, it finds that NDV down-regulates expression of genes like estrogen receptor 1 (ESR1) and up-regulates genes like Bcl-2 binding component 3 (BBC3), both of which are involved in apoptosis.
3) Cell cycle analysis shows that 11% and 41% of MCF-7 cells underwent apoptosis at 3 and 6 hours post-infection respectively, indicating NDV induces apoptosis in the cancer cellsRole of Sema4D in Bone Metastasis of Breast Cancer
Role of Sema4D in Bone Metastasis of Breast CancerANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY
1. The study explored the role of Sema4D in bone metastasis of breast cancer. Sema4D expression was downregulated in MCF-7 breast cancer cells using siRNA transfection. Proliferation, invasion, and osteoblast differentiation were then measured.
2. Proliferation and invasion were reduced in the Sema4D siRNA group compared to control groups. Bone nodule area and AKT phosphorylation were increased in the Sema4D siRNA group.
3. Downregulating Sema4D expression attenuated inhibition of osteoblast differentiation, likely by enhancing AKT phosphorylation. Sema4D may play a role in regulating bone metastasis of breast cancer.Identifying novel and druggable targets in a triple negative breast cancer ce...
Identifying novel and druggable targets in a triple negative breast cancer ce...Thermo Fisher Scientific
In this study, we developed a CRISPR/Cas9-based high throughput loss-of-function screen for identifying target genes responsible for the tumor proliferation and growth in TNBC. Our initial focus was to identify essential kinases in MDA-MB-231 cell line using the Invitrogen™ LentiArray™ Human Kinase CRISPR Library, which targets 840 kinases with up to 4 different gRNAs per protein kinase for complete gene knockout. This functional screen identified over 90 protein kinases that are essential for cell viability and cell proliferation. Ten of these hits (CDK1, CDK2, CDK8, CDK10, CDK11A, CDK19, CDK19, CDC7, EPHA2 and WEE1) are well-known targets validated in the literature. Currently, we are in the process validating the novel hits through target gene sequencing, western blotting and target specific small molecule kinase inhibitors.Oncolytic Virus Therapy Development - Creative Biolabs
Oncolytic virotherapy is cancer treatment using a native or reprogrammed virus that has the potential to targeting and killing cancerous cell. Taking advantage of the OncoVirapy™ platform, Creative Biolabs provides customized, standardized, and reliable and high-quality oncolytic virus therapy development services for clients globally.
Provenge (sipuleucel t)
Sipuleucel-T (Provenge) is an autologous cellular immunotherapy for asymptomatic metastatic prostate cancer. It consists of autologous dendritic cells activated ex vivo with a recombinant fusion protein and infused back to the patient to stimulate an immune response against prostate cancer cells. Clinical trials demonstrated a significant survival benefit for patients treated with Sipuleucel-T compared to placebo. Dendreon is preparing for the commercial launch of Provenge in the US and EU, which will require a major manufacturing and logistics effort to process and deliver the personalized immunotherapy to thousands of patients.
KIYATEC-Advanced-CoCulture-LRes
KIYATEC has developed advanced 3D co-culture techniques to model tumor-immune cell interactions in vitro. Their models can maintain viability of primary breast cancer cultures for 64 days. They have created tri-, tetra-, and penta-culture models incorporating tumor cells and immune components to test immuno-oncology drugs. KIYATEC aims to develop the most clinically relevant personalized tumor models to assess immune therapies and targeted drugs.
Suppress lung cancer progression via up regulation of linc rna-p21
Suppress lung cancer progression via up regulation of linc rna-p21Clinical Surgery Research Communications
Radiotherapy promotes the polarization of tumor-associated macrophages (TAMs) in mice with Lewis lung cancer into anti-tumor M1 macrophages. This is accompanied by increased expression of the long non-coding RNA lincRNA-p21 in the TAMs. TAMs exposed to radiation therapy suppress the viability and invasion of Lewis lung cancer cells in culture. Overexpression of lincRNA-p21 in TAMs enhances their anti-tumor effects, while decreasing lincRNA-p21 reduces the effects of radiation therapy, suggesting lincRNA-p21 plays a key role in the anti-tumor actions of radiotherapy in lung cancer.Preclinical trial for Anticancer drugs
Screening methods for Anticancer drugs. Description of Principles and procedures of screening methods for anticancer drugs.
miR-138-5p Promotes Proliferation of Human Melanoma Cells by Inhibiting hTERT...
miR-138-5p Promotes Proliferation of Human Melanoma Cells by Inhibiting hTERT...ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY
This study examined the role of miR-138-5p in regulating human melanoma cell proliferation. The study found that miR-138-5p expression was significantly higher in melanoma tissues compared to controls. Overexpression of miR-138-5p promoted proliferation of Me45 melanoma cells, while inhibition of miR-138-5p suppressed proliferation. Bioinformatics analysis predicted that miR-138-5p targets the 3'-UTR of hTERT, and luciferase assays confirmed this. Knockdown of hTERT reversed the promotive effects of miR-138-5p on cell proliferation, indicating miR-138-5p regulates proliferation by directly targeting hTERT. Therefore, this study demonstrates that miR-138-5Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Product...
Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or
GP51/61) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype
determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for
substantive HPV-type discrimination. A reverse blot method was developed which employs a biotin-labeled
PCR product hybridized to an array of immobilized oligonucleotide probes. By the reverse blot strip analysis,
genotype discrimination of multiple HPV types can be accomplished in a single hybridization and wash cycle.
Twenty-seven HPV probe mixes, two control probe concentrations, and a single reference line were immobilized
to 75- by 6-mm nylon strips. Each individual probe line contained a mixture of two bovine serum albuminconjugated
oligonucleotide probes specific to a unique HPV genotype. The genotype spectrum discriminated on
this strip includes the high-risk, or cancer-associated, HPV genotypes 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 55,
56, 58, 59, 68 (ME180), MM4 (W13B), MM7 (P291), and MM9 (P238A) and the low-risk, or non-cancerassociated,
genotypes 6, 11, 40, 42, 53, 54, 57, 66, and MM8 (P155). In addition, two concentrations of b-globin
probes allowed for assessment of individual specimen adequacy following amplification. We have evaluated the
performance of the strip method relative to that of a previously reported dot blot format (H. M. Bauer et al.,
p. 132–152, in C. S. Herrington and J. O. D. McGee (ed.), Diagnostic Molecular Pathology: a Practical Approach,
(1992), by testing 328 cervical swab samples collected in Digene specimen transport medium (Digene Diagnostics,
Silver Spring, Md.). We show excellent agreement between the two detection formats, with 92%
concordance for HPV positivity (kappa 5 0.78, P < 0.001). Nearly all of the discrepant HPV-positive samples
resulted from weak signals and can be attributed to sampling error from specimens with low concentrations
(<1 copy/ml) of HPV DNA. The primary advantage of the strip-based detection system is the ability to rapidly
genotype HPVs present in genital samples with high sensitivity and specificity, minimizing the likelihood of
misclassification.
Emanuel Zapata Gutiérrez Tercer semestre - medicina Universidad Pontificia_20...
This document discusses colorectal cancer and the evaluation of tumor metastasis and CD8+ T cell infiltration through the circRNF216/miR-576-5p/ZC3H12C axis in colorectal cancer. Several methods were used in the study including western blot assays, quantitative real-time PCR, luciferase reporter assays, and immunohistochemistry on lung tissue to detect specific proteins. Results from previous studies on circular RNAs, CD8 tumor-infiltrating lymphocytes, and sensing of circular RNAs by intron identity were discussed. The conclusion is that molecular biology allows for more precise identification and characterization of diseases at the molecular level and provides fundamental information about disease molecular mechanisms.
Provenge (Sipuleucel T)
1. Sipuleucel-T (Provenge) is an autologous cellular immunotherapy for asymptomatic metastatic prostate cancer that works by activating antigen-presenting cells and T-cells against prostatic acid phosphatase.
2. Clinical trials showed Provenge improved overall survival in metastatic castration-resistant prostate cancer patients.
3. Manufacturing and delivering Provenge presents logistical challenges due to its personalized nature that Dendreon aims to address through an advanced planning system and partnerships.
Similar to FNP workshop presentation (20)
Effect of miR-21 on Oral Squamous Cell Carcinoma Cell Proliferation and Apopt...
Effect of miR-21 on Oral Squamous Cell Carcinoma Cell Proliferation and Apopt...
Mi r 449b inhibits the migration and invasion of colorectal cancer cells thro...
Mi r 449b inhibits the migration and invasion of colorectal cancer cells thro...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Role of Sema4D in Bone Metastasis of Breast Cancer
Role of Sema4D in Bone Metastasis of Breast Cancer
Identifying novel and druggable targets in a triple negative breast cancer ce...
Identifying novel and druggable targets in a triple negative breast cancer ce...
Oncolytic Virus Therapy Development - Creative Biolabs
Oncolytic Virus Therapy Development - Creative Biolabs
Suppress lung cancer progression via up regulation of linc rna-p21
Suppress lung cancer progression via up regulation of linc rna-p21
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miR-138-5p Promotes Proliferation of Human Melanoma Cells by Inhibiting hTERT...
Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Product...
Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Product...
Emanuel Zapata Gutiérrez Tercer semestre - medicina Universidad Pontificia_20...
Emanuel Zapata Gutiérrez Tercer semestre - medicina Universidad Pontificia_20...
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By harnessing the power of High Flux Vacuum Membrane Distillation, Travis Hills from MN envisions a future where clean and safe drinking water is accessible to all, regardless of geographical location or economic status.
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
fermented food science of sauerkraut.pptx
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Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
Alternate Wetting and Drying - Climate Smart Agriculture
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PPT on Alternate Wetting and Drying presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024. Direct Seeded Rice - Climate Smart Agriculture
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PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
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Isolation of pure culture, its various method.
Randomised Optimisation Algorithms in DAPHNE
Slides from talk:
Aleš Zamuda: Randomised Optimisation Algorithms in DAPHNE .
Austrian-Slovenian HPC Meeting 2024 – ASHPC24, Seeblickhotel Grundlsee in Austria, 10–13 June 2024
https://ashpc.eu/
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...
Wereport the study of a huge optical intraday flare on 2021 November 12 at 2 a.m. UT in the blazar OJ287. In the binary black hole model, it is associated with an impact of the secondary black hole on the accretion disk of the primary. Our multifrequency observing campaign was set up to search for such a signature of the impact based on a prediction made 8 yr earlier. The first I-band results of the flare have already been reported by Kishore et al. (2024). Here we combine these data with our monitoring in the R-band. There is a big change in the R–I spectral index by 1.0 ±0.1 between the normal background and the flare, suggesting a new component of radiation. The polarization variation during the rise of the flare suggests the same. The limits on the source size place it most reasonably in the jet of the secondary BH. We then ask why we have not seen this phenomenon before. We show that OJ287 was never before observed with sufficient sensitivity on the night when the flare should have happened according to the binary model. We also study the probability that this flare is just an oversized example of intraday variability using the Krakow data set of intense monitoring between 2015 and 2023. We find that the occurrence of a flare of this size and rapidity is unlikely. In machine-readable Tables 1 and 2, we give the full orbit-linked historical light curve of OJ287 as well as the dense monitoring sample of Krakow.
Summary Of transcription and Translation.pdf
Hello Everyone Here We are Sharing You with The process of protien Synthesis in very short points you will be Able to understand It Very well
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Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
Discovery of An Apparent Red, High-Velocity Type Ia Supernova at 𝐳 = 2.9 wi...
We present the JWST discovery of SN 2023adsy, a transient object located in a host galaxy JADES-GS
+
53.13485
−
27.82088
with a host spectroscopic redshift of
2.903
±
0.007
. The transient was identified in deep James Webb Space Telescope (JWST)/NIRCam imaging from the JWST Advanced Deep Extragalactic Survey (JADES) program. Photometric and spectroscopic followup with NIRCam and NIRSpec, respectively, confirm the redshift and yield UV-NIR light-curve, NIR color, and spectroscopic information all consistent with a Type Ia classification. Despite its classification as a likely SN Ia, SN 2023adsy is both fairly red (
�
(
�
−
�
)
∼
0.9
) despite a host galaxy with low-extinction and has a high Ca II velocity (
19
,
000
±
2
,
000
km/s) compared to the general population of SNe Ia. While these characteristics are consistent with some Ca-rich SNe Ia, particularly SN 2016hnk, SN 2023adsy is intrinsically brighter than the low-
�
Ca-rich population. Although such an object is too red for any low-
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cosmological sample, we apply a fiducial standardization approach to SN 2023adsy and find that the SN 2023adsy luminosity distance measurement is in excellent agreement (
≲
1
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) with
Λ
CDM. Therefore unlike low-
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Ca-rich SNe Ia, SN 2023adsy is standardizable and gives no indication that SN Ia standardized luminosities change significantly with redshift. A larger sample of distant SNe Ia is required to determine if SN Ia population characteristics at high-
�
truly diverge from their low-
�
counterparts, and to confirm that standardized luminosities nevertheless remain constant with redshift.
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GBSN - Biochemistry (Unit 6) Chemistry of Proteins
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The debris of the ‘last major merger’ is dynamically young
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FNP workshop presentation
- 1. New therapy for cancer by genetically modified strain of Salmonella Anshu Rastogi, Paulina chorobik, Joanna Bereta Department of Cell Biochemistry; Faculty of Biochemistry, Biophysics and Biotechnology; Jagiellonian University; Krakow
- 2. Introduction: = Healthy Cell = Tumor Cell = Apoptotic Cell = Immune Cell = Bacteria = Bacterial protein Objective: Development of a bacterial strain, which will be effective in eradication of tumor cells. Test the effectiveness of strain on tumor cell lines and animal models.
- 3. Material and Methods: The Salmonella Typhimurium strain VNP20009 was engineered for the expression and production of SipB protein (A protein responsible for apoptosis of animal cells). Tumor cell lines used: MC-38 CEA, RAW 264.7 Methods used: Viable cell counting by cell counter. Tunel assay for the detection of apoptotic cells. Results and discussion: RAW RAWS+ RAWS- RAWS+0.5 RAWS-0.5 0 5 10 15 20 25 30 35 40 45 Numberofviablecells(X30000) Condition
- 4. MC38 T MC38 S+ T MC38 S- T MC38 DNase T
- 5. Future Plan: To test the effectiveness of modified strain of Salmonella on other tumor cell lines and animal models such as mouse and mini pig. Acknowledgement: This work was supported by the Polish National Centre for research and development through INNOTECH grant number 152553. Thank You Anshu.rastogi@uj.edu.pl
- 6. Future Plan: To test the effectiveness of modified strain of Salmonella on other tumor cell lines and animal models such as mouse and mini pig. Acknowledgement: This work was supported by the Polish National Centre for research and development through INNOTECH grant number 152553. Thank You Anshu.rastogi@uj.edu.pl