The document discusses tumor immunology and tumor markers. It provides information on:
1) How tumors form from normal cells through mutations, carcinogens or viruses and become antigenically different. The immune system normally recognizes and destroys these cells.
2) Mechanisms by which tumors can escape immune detection including loss of antigen presentation or production of immunosuppressive factors.
3) How the immune system acts as a surveillance system to detect and eliminate neoplastic cells through natural killer cells, cytotoxic T-cells, antibodies and complement activation.
4) Tumor markers that can be used for diagnosis and monitoring treatment including tumor antigens like alpha-fetoprotein and carcinoembryonic antigen, and tumor
This presentation deals with the concept of Tumor immunity. It cover different types of Tumor and tumor antigens. How immunology plays role in tumor detection and diagnosis is also explained in this presentation. Concept of Tumor imunoprophylaxis and tumor immunotherapy is also explained.
Your immune system and cancer - Jonathan Cebon - BreaCan 5 August 2015BreaCan
Professor Cebon discusses the link between the immune system and cancer, immunotherapy and the current research being undertaken to develop new treatments that target cancer cells. This session was jointly hosted by BreaCan and Olivia Newton-John Cancer and Wellness Centre.
This presentation deals with the concept of Tumor immunity. It cover different types of Tumor and tumor antigens. How immunology plays role in tumor detection and diagnosis is also explained in this presentation. Concept of Tumor imunoprophylaxis and tumor immunotherapy is also explained.
Your immune system and cancer - Jonathan Cebon - BreaCan 5 August 2015BreaCan
Professor Cebon discusses the link between the immune system and cancer, immunotherapy and the current research being undertaken to develop new treatments that target cancer cells. This session was jointly hosted by BreaCan and Olivia Newton-John Cancer and Wellness Centre.
What is immunology?
What is Tumor?
Types of tumor
Classification of Malignant tumors
Malignant transformation of cells
General features of Tumor immunity
Tumor antigens
Tumor specific antigen
Tumor associated antigens
Immune response to tumor
Evasion of immune response by tumor
Cancer Immunosurveillance versus Immunoediting
Immunotechniques
RIA
ELISA
The presentation outlines aspects of immunity against cancer, evasion strategies by cells, immunotherapy in cancer, cancer vaccines etc. Download and view the slideshow for better experience.
Prepared in Sept 2014
Cytokine-tumor interactions within its microenvironment play a critical role in pathogenesis and management of any neoplasm. Here, I summarize points from a 2004 Nature paper that are still pertinent today.
Slide Template: www.presentationmagazine.com
Tumor, Tumor immunology, cancer, hallmarks of cancer, carcinoma, lymphoma, metastasis, malignant, benign, angiogenesis, oncogenes and cancer induction, kuby detailed study quick revision, proto-oncogenes, tumor antigens, antibody, experiments for tumor antigens, methods for characterization of TSTA, Immunoediting, Current research n new approaches, monoclonal antibody
IMMUNE RESPONSE TO TUMORS-Humoral immunity
-Cellular Immunity- Failure of Host Defenses
- Evasion of Immune Responses by Tumors
- Cancer Immunosurveillance vs Immunoediting- Immunotherapy
What is immunology?
What is Tumor?
Types of tumor
Classification of Malignant tumors
Malignant transformation of cells
General features of Tumor immunity
Tumor antigens
Tumor specific antigen
Tumor associated antigens
Immune response to tumor
Evasion of immune response by tumor
Cancer Immunosurveillance versus Immunoediting
Immunotechniques
RIA
ELISA
The presentation outlines aspects of immunity against cancer, evasion strategies by cells, immunotherapy in cancer, cancer vaccines etc. Download and view the slideshow for better experience.
Prepared in Sept 2014
Cytokine-tumor interactions within its microenvironment play a critical role in pathogenesis and management of any neoplasm. Here, I summarize points from a 2004 Nature paper that are still pertinent today.
Slide Template: www.presentationmagazine.com
Tumor, Tumor immunology, cancer, hallmarks of cancer, carcinoma, lymphoma, metastasis, malignant, benign, angiogenesis, oncogenes and cancer induction, kuby detailed study quick revision, proto-oncogenes, tumor antigens, antibody, experiments for tumor antigens, methods for characterization of TSTA, Immunoediting, Current research n new approaches, monoclonal antibody
IMMUNE RESPONSE TO TUMORS-Humoral immunity
-Cellular Immunity- Failure of Host Defenses
- Evasion of Immune Responses by Tumors
- Cancer Immunosurveillance vs Immunoediting- Immunotherapy
A presentation descripes tumors,pathogensis,devlopment,antigenes and genes.
how host responds to them and how tumors evade immunity with latest lines of therapy and prevention.
facaulity of pharmacy.Damascus university.master of libaratory diagnossis. immunology.
Baraa ALomar and feras deban
Patients are beginning to benefit from antibody based, cellular and vaccine approaches that are effective against genetically diverse and therapy-resistance cancers.
El 3 de noviembre de 2015, la Fundación Ramón Areces organizó en su sede en Madrid (C/ Vitruvio, 5) una jornada sobre ‘El cáncer como consecuencia del envejecimiento: posibles soluciones’. Coordinado por la investigadora María Vallet Regí, del Departamento de Química Inorgánica y Bioinorgánica de la Universidad Complutense de Madrid, contó con la presencia, entre otros científicos, de Mariano Barbacid, Lodovico Balducci y Theresa Guise.
Chemotherapy: Imperfect
Systematic nature of cytoxicity
Agents lack intrinsic anti-tumor selectivity
Anti-proliferative mechanism on cells in cycle, rather than specific toxicity directed towards particular cancer cell
Host toxicity: treatment discontinued at dose levels well below dose required to kill all viable tumor cells
One of the most critical roles performed by fibroblasts, both in normal and cancer tissue, is the production and remodeling of the extracellular matrix (ECM). Not only does the ECM impart structural support and strength to tissues, it also provides attachment sites for cell surface receptors, and functions as a reservoir of cytokines and other growth factors27The structure of tumor-associated ECM is abnormal, with loose structure and disorganized collagen fibers28Matrix metalloproteinases (MMPs) are a large family of enzymes capable of degrading components of the ECM and are critical in maintenance of the ECM. Degradation of the ECM by MMPs releases growth factors, enhances migration, and alters cell:cell and cell:ECM interactions29. Although MMPs can be produced by tumor cells, most are produced by fibroblasts and macrophages, and high levels of MMPs are found at the tumor:stroma interface7. Because MMPs are secreted into the surrounding environment by these cells, they are a good example of the interaction that occurs between a tumor and its environment.
Evidence indicates that MMPs are key players in multiple steps of tumor progression; they promote metastasis, angiogenesis, and even tumor initiation. One of the many paradoxes of MMP activity is that MMPs often have opposing effects depending on the composition of the tumor environment and the nature of MMPs present. For example, MMPs can either promote or inhibit angiogenesis, depending on the molecules they release from the ECM3029. Because of their potent effects on tumor formation and metastasis, several clinical trials attempted to use MMP inhibitors as anticancer therapy. However, these trials were soon stopped as patients developed muscle and bone pain, formed connective tissue nodules, and developed joint disorders. These trials highlight the difficulty of targeting molecules critical for the function of multiple tissues
The Tumor Stroma and Metastasis
• Seed and Soil hypothesis: given tumor cells (seeds) can only colonize particular distant tissues (soil) that have a suitable growth environment.
• Two key events must occur for site-specific metastasis to occur: 1) formation of a viable landing spot and 2) expression of appropriate genes in the tumor cells.
• Tumor cells may invade foreign tissue but fail to colonize it. The reasons for this are unknown. These cells are considered 'dormant' cancer cells.
3. Tumor immunologyTumor immunology
** Pathological cell massesPathological cell masses derived by abnormal andderived by abnormal and
uncontrollable clonal expansion of single celluncontrollable clonal expansion of single cell
* Transformation of normal cells to malignant cells by:* Transformation of normal cells to malignant cells by:
a-a- Spontaneous mutationSpontaneous mutation during daily cell divisionduring daily cell division
chemical carcinogenschemical carcinogens
b- It may beb- It may be induced byinduced by physical carcinogensphysical carcinogens
virusesviruses
* Cells become* Cells become antigenically differentantigenically different from normal cellsfrom normal cells
* They are* They are recognizedrecognized andand destroyeddestroyed byby immune systemimmune system
4. Etiology Of TumorEtiology Of Tumor
1) Inherited :1) Inherited :
Expression of inherited oncogeneExpression of inherited oncogene
e.g. viral gene incorporated into host genee.g. viral gene incorporated into host gene
2) Viral:2) Viral:
- Human papilloma, herpes type 2, HBV, EBV (DNA)- Human papilloma, herpes type 2, HBV, EBV (DNA)
- Human T-cell leuckemia virus (RNA)- Human T-cell leuckemia virus (RNA)
3) Chemical:3) Chemical:
- Poly cyclic hydrocarbons cause sarcomas- Poly cyclic hydrocarbons cause sarcomas
- Aromatic amines cause mammary carcinoma- Aromatic amines cause mammary carcinoma
- Alkyl nitroso amines cause hepatoma- Alkyl nitroso amines cause hepatoma
4) Radiological:4) Radiological: Ultraviolet & ionizing irradiationUltraviolet & ionizing irradiation
5) Spontaneous:5) Spontaneous: failure in the cellular growth controlfailure in the cellular growth control
5. Tumor Associated AntigensTumor Associated Antigens
!) Viral Antigen :!) Viral Antigen :
a- Viral proteins and glycoproteinsa- Viral proteins and glycoproteins
b- New antigens produced by virally infected hostb- New antigens produced by virally infected host
cells under control of viral nucleic acidcells under control of viral nucleic acid
2) Tumor specific antigens :2) Tumor specific antigens :
- Tumor cells develop new antigen specific to- Tumor cells develop new antigen specific to
their carcinogenstheir carcinogens
3) Tumor specific transplantation antigens :3) Tumor specific transplantation antigens :
- Tumor cells express new MHC antigens due to- Tumor cells express new MHC antigens due to
alteration of normally present MHC antigensalteration of normally present MHC antigens
6. Tumor Associated AntigensTumor Associated Antigens
4) Oncofetal antigens:4) Oncofetal antigens:
a- Carcino-embryonic antigens (CEA)a- Carcino-embryonic antigens (CEA)
- Normally expressed during fetal life on fetal gut- Normally expressed during fetal life on fetal gut
- Reappearance in adult life:- Reappearance in adult life:
GIT, pancreas, biliary system and cancer breastGIT, pancreas, biliary system and cancer breast
b- Alpha fetoprotein:b- Alpha fetoprotein:
- Normally expressed in fetal life- Normally expressed in fetal life
- Reappearance in adult life; hepatoma- Reappearance in adult life; hepatoma
7. Immune Surveillance SystemImmune Surveillance System
* During neoplastic transformation, new antigen develop* During neoplastic transformation, new antigen develop
* The host recognize them as nonself antigens* The host recognize them as nonself antigens
* Cell mediated immune reactions attack these* Cell mediated immune reactions attack these
nonself tumor cellsnonself tumor cells
* Immune response act as surveillance system* Immune response act as surveillance system
to detect and eliminate newly arising neoplastic cellsto detect and eliminate newly arising neoplastic cells
8. Immune Surveillance SystemImmune Surveillance System
This system include :This system include :
1) Natural killer (NK) cells1) Natural killer (NK) cells
They kill directly tumor cells,helped by interferon, IL-2They kill directly tumor cells,helped by interferon, IL-2
2) Cytotoxic T-cells2) Cytotoxic T-cells
They also kill directly tumor cellsThey also kill directly tumor cells
3) Cell mediated T-cells (effector T-cells)3) Cell mediated T-cells (effector T-cells)
They produce and release a variety of lymphokines :They produce and release a variety of lymphokines :
a-Macrophage activation factora-Macrophage activation factor that activate macrophagthat activate macrophag
b-Gamma interferon and interleukin-2b-Gamma interferon and interleukin-2 that activate NKthat activate NK
c-Tumor necrosis factor (cachectine)c-Tumor necrosis factor (cachectine)
9. Immune Surveillance SystemImmune Surveillance System
4) B-cells :4) B-cells :
- Tumor associated antigens stimulate production of- Tumor associated antigens stimulate production of
specific antibodies by host B-cellsspecific antibodies by host B-cells
- These specific antibodies bind together on tumor cell surface- These specific antibodies bind together on tumor cell surface
leading to destruction of tumor through:leading to destruction of tumor through:
a- Antibody mediated-cytotoxicity :a- Antibody mediated-cytotoxicity :
killkill
Cytotoxic T-cells IgG-coated tumor cellsCytotoxic T-cells IgG-coated tumor cells
b- Activation of macrophagesb- Activation of macrophages
releaserelease
Sensitized T-cells macrophage activating factorSensitized T-cells macrophage activating factor
IgG-coated tumor cells macrophagesIgG-coated tumor cells macrophages
activateactivate
c- Activation of classical pathway of complementc- Activation of classical pathway of complement leading toleading to
10. Tumor EscapeTumor Escape
Mechanisms by which tumor escape immune defenses:Mechanisms by which tumor escape immune defenses:
1) Reduced levels or absence of MHCI molecule1) Reduced levels or absence of MHCI molecule onon
tumor so that they can not be recognized by CTLstumor so that they can not be recognized by CTLs
2) Some tumors stop expressing the antigens2) Some tumors stop expressing the antigens
These tumors are called “antigen loss variants”These tumors are called “antigen loss variants”
3) Production of immunosuppressive factors by tumor3) Production of immunosuppressive factors by tumor
e.g. transforming growth factor (TGF-e.g. transforming growth factor (TGF-ββ))
4) Tumor antigens may induce specific immunologic4) Tumor antigens may induce specific immunologic
tolerancetolerance
11. Tumor EscapeTumor Escape
5) Tumor cells have an inherent defect in antigen5) Tumor cells have an inherent defect in antigen
processing and presentationprocessing and presentation
6) Blocking of receptors on T-cells by specific antigen6) Blocking of receptors on T-cells by specific antigen
antibodies complex (antibodies complex (after shedding of tumor Ag)after shedding of tumor Ag)
prevents them from recognizing and attackingprevents them from recognizing and attacking
tumor cellstumor cells
7) Antigens on the surface of tumors may be masked7) Antigens on the surface of tumors may be masked
by sialic acid-containing mucopolysaccharidesby sialic acid-containing mucopolysaccharides
8) Immune suppression of the host as in transplant8) Immune suppression of the host as in transplant
patientspatients who show a higher incidence of malignancywho show a higher incidence of malignancy
12. Tumor MarkersTumor Markers
* Tumor markers :* Tumor markers :
Tumor antigensTumor antigens
* They are either or* They are either or
Tumor productsTumor products
(enzymes and hormones)(enzymes and hormones)
* Tumor products are released in the serum of patients* Tumor products are released in the serum of patients
* They are used to* They are used to confirm diagnosisconfirm diagnosis andand follow upfollow up thethe
response toresponse to therapytherapy
13. Tumor AntigensTumor Antigens
1) Alpha fetoprotein antigen (AFP)1) Alpha fetoprotein antigen (AFP) in cases of hepatomain cases of hepatoma
2) Carcinoembryoinic antigen (CEA)2) Carcinoembryoinic antigen (CEA) in gastrointestinalin gastrointestinal
tumors, tumors of biliary system and cancer breasttumors, tumors of biliary system and cancer breast
3) Cancer antigen 1253) Cancer antigen 125 (CA 125)(CA 125) in ovarian carcinomain ovarian carcinoma
4) Cancer antigen 15-3 (CA15-3)4) Cancer antigen 15-3 (CA15-3) in breast cancerin breast cancer
5) Cancer antigen 19-95) Cancer antigen 19-9 in colon and pancreatic tumorin colon and pancreatic tumor
6) Prostatic specific antigen (PSA)6) Prostatic specific antigen (PSA) in prostatic tumorsin prostatic tumors
14. Tumor ProductsTumor Products
a) Hormones :a) Hormones :
-- Human chorionic gonadotrophins (HCG)Human chorionic gonadotrophins (HCG) are secretedare secreted
in cases of choriocarcinomain cases of choriocarcinoma
- Thyroxin (T3 & T4)- Thyroxin (T3 & T4) is secreted in cases of canceris secreted in cases of cancer
of thyroid glandof thyroid gland
b) Enzymes :b) Enzymes :
- Acid phosphatase- Acid phosphatase enzymes in cases of cancer prostaeenzymes in cases of cancer prostae
- Alkaline phosphatese, lipase and amylase- Alkaline phosphatese, lipase and amylase enzymes inenzymes in
cases of cancer pancreascases of cancer pancreas