This document discusses research into the role of the Bmi1 gene in tongue squamous cell carcinoma (TSCC). The study evaluated Bmi1 expression in 77 TSCC tumor samples and its impact on cancer cell migration and invasion. The results showed high Bmi1 expression in TSCC tissues and cell lines. Silencing Bmi1 using siRNA inhibited the migration and invasion of TSCC cells. Additionally, the study found that Bmi1 expression may be regulated by the C-myc oncogene and that both Bmi1 and C-myc regulate genes involved in cell proliferation and metastasis. This research provides insights into how Bmi1 contributes to TSCC development and progression.

1. Laura Navarrete Morales.
Manuela Posada Muñoz.

2.  Cancer: is the uncontrolled growth and
spread of cells.
 Carcinoma: is a tipe of cancer in epitelial
cells.
* Squamous cell carcinoma (SCC): is in the
squamous cells, which compose most of the
skin’s upper layers (the epidermis).

3. Has been reported as an
oncogene by
regulating p16 and p19,
which are cell cycle inhibitor
genes.
Is necessary for efficient self-
renewing cell divisions of
adult hematopoietic stem
cells as well as adult peripheral
and central nervous system
neural stem cells.

4. As an oncogenic transcription factor,
this protein recognizes an E-box
recognition site in the promoter of its
target genes and then exerts a wide
array of biological
functions in different cellular
models, including cell cycle control,
cell differentiation,
and metastasis.

5. -C-myc-Bmi1-SOD2
-C-myc regulate the expression of an
unusually large number of target genes,
including Bmi1.

6.  “The purpose of this study
is to evaluate the role of
Bmi1 in the development of
tongue squamous cell
carcinoma (TSCC) and its
functional effect on the
migration and invasion of
TSCC.”

7. -77 casos de TSCC ⇨22
casosde lesiones premalignas
12 lengua normal.
- Seguimiento a 52 casos

8. -Fue usado el anticuerpo anti-Bmi1.
-La evaluación se realizó por
inmunotinción, por 3 patólogos
diferentes.
-Escala de inmunotinción de 0-3

9. -Celular ⇨ UM1 y UM2
-Medio de cultivo ⇨ DMEM/F12
-Gen de transfección ⇨ SiRNA.

10. -Células sembradas en la
cámara superior del
dispositivo.
-Luego de 24h las células en
la superficie inferior eran
fijadas y teñidas con la
solución DAPI en la oscuridad.
-Las de la parte superior de la cámara se
invadieron con matrigel

11. -Las proteínas son separadas por
electroforesis en gel según su tamaño, por
medio de la técnica SDS/PAGE.
-Hecha la electroforesis se transfieren a un
filtro que se incuba con anticuerpos que
reaccionan con las proteínas de interés.
-Bmi1, SOD2, Slug, Nanog, C-myc, ABCG2 y
GAPDH

12. TSCC -> incubación en la oscuridad ->
verapamil e inhibidor de transportadores ABC -
> centrifugación -> resuspención ->
separación celular -> exclusión de células
muertas -> fluorescencia
24 placas de filtración ultra baja con 1000 células/ml
en DMEM/F12 con bFGF, EGF y un antimicótico,
calculada en 14 días -> tinción con giemsa

13. Imnunoprecipitación: formaldehído ->
incubación con C-myc -> recuperación
de ADN libre -> PCR -> estudio de
primers de Bmi1

14. -Gen informador de
luciferasa para Bmi1
-Un plásmido que contiene
C-myc

15. Incubación en ckk-8

22. AUTHORS COMMENT
Liu W, Feng JQ, Shen XM, et al ATP-binding cassette, G2 subfamily
(ABCG2) and BMI-1, predict the
transformation of oral leukoplakia to
cancer: a long term follow up study
Feng JQ, Xu ZY, Shi LJ, et al Expression of cancer stem cell
markers ALDH1 and Bmi1 in oral
erythroplakia and the risk of oral
cancer
Hayry V, Makinen LK, Atula T, et
al
Bmi-1 expression predicts prognosis
in squamous cell carcinoma of the
tongue
Liu X, Wang A, Lo Muzio L, et al. Deregulation of manganese
superoxide dismutase (SOD2)
expression and lymph node
metastasis in tongue squamous cell
carcinoma

23. With this article, all the people
who work against cancer, can
learn how the Bmi1 mediated
migration and invasion of TSCC,
and now they can work against
this oncogene.
Is important to know the different
mechanisms that have cells to
regulate, this could prevent an
inappropriate proliferation of the
same.

24. The expression of Bmi1 may be
regulated by C-myc, with this
Knowledge the scientists, the doctors
and the biopharmaceuticals are going
to study this mechanism to neutralize
the action of the Bmi1
the squamous cell carcinoma is
associated with the mutation of the
Bmi1 and the SDO2. These are
normal in the body but they
overexpression are causing cancer
and premature aging

25. Manuela Posada.

26. Laura Navarrete.