DNA fingerprinting is a technique used to distinguish between individuals of the same species using DNA samples. It was invented by Alex Jeffreys in 1985. There are 8 key steps to DNA fingerprinting including isolating DNA from samples, digesting the DNA with restriction enzymes, separating DNA fragments via electrophoresis, transferring fragments to a membrane, probing the membrane, hybridization, autoradiography to detect patterns, and interpreting band patterns. DNA fingerprinting is used for identifying criminals, determining paternity, and personal identification.
This presentation is about DNA fingerprinting, a brief description is given about its principle, working, technique and its application with a example.
This presentation is about DNA fingerprinting, a brief description is given about its principle, working, technique and its application with a example.
This presentation will help you in understanding what is a pollen grain, what is forensic palynology, Structure of Pollen grain, It's biology, Dispersal and production, How to identify a pollen grain to species level based on Morphological characteristics and along with imparting detailed knowledge it will also help you to understand it's forensic significance.
DNA fingerprinting is a research facility procedure used to set up a connection between natural proof and a suspect in a criminal examination. A DNA test taken from a wrongdoing scene is contrasted and a DNA test from a suspect. On the off chance that the two DNA profiles are a match, at that point the proof originated from that suspect. On the other hand, on the off chance that the two DNA profiles don't coordinate, at that point the proof can't have originated from the suspect. DNA fingerprinting is likewise used to build up paternity.
The technique of molecular biology like DNA isolation, RNA isolation, PCR, Western blot, RFLP, etc was developed with development in science. This presentation includes the method of DNA and RNA isolation and their Quantification techniques.
This presentation will help you in understanding what is a pollen grain, what is forensic palynology, Structure of Pollen grain, It's biology, Dispersal and production, How to identify a pollen grain to species level based on Morphological characteristics and along with imparting detailed knowledge it will also help you to understand it's forensic significance.
DNA fingerprinting is a research facility procedure used to set up a connection between natural proof and a suspect in a criminal examination. A DNA test taken from a wrongdoing scene is contrasted and a DNA test from a suspect. On the off chance that the two DNA profiles are a match, at that point the proof originated from that suspect. On the other hand, on the off chance that the two DNA profiles don't coordinate, at that point the proof can't have originated from the suspect. DNA fingerprinting is likewise used to build up paternity.
The technique of molecular biology like DNA isolation, RNA isolation, PCR, Western blot, RFLP, etc was developed with development in science. This presentation includes the method of DNA and RNA isolation and their Quantification techniques.
DNA FINGERPRINTING :-
“DNA fingerprinting is a technique that shows the genetic makeup of living things. It is a method of finding the difference between the satellite DNA regions in the genome.”
Content:-
1) Introduction : What is DNA Fingerprinting?
2) Biological samples used
3) Discovery : Who invented DNA Fingerprinting?
4) Procedure : Basic steps and procedure for DNA Fingerprinting
5) Application : Various applications of DNA Fingerprinting
The term DNA Finger printing is also known as DNA Typing, Genetic Profiling or Genotyping, it is a process in which the DNA characteristics of a person is determined by isolating and identifying variable elements in the base-pair sequence of DNA.
By developing this method in 1984 the British geneticist Alec Jeffery found that some sequence area unit extremely variable Deoxyribonucleic acid called as minisatellites. These minisatellites do not have contribution in functioning of DNA and are repeated in the genes. Geneticist found that in every person there is a unique pattern of these minisatellites except the identical twins.
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This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
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Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
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Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
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The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
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2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
2. DNA Fingerprinting is a
way to identify a certain
individual, rather than
simply identifying a
species or a particular
trait.
A technique used by
scientists to distinguish
between individuals of
the same species using
only samples of their
DNA
3. The process of DNA
fingerprinting was
invented by Alex
Jeffreys in 1985.
4. Blood
Hair
Saliva
Semen
Body tissue cells
DNA samples have
been obtained from
vaginal cells transferred
to the outside of a
condom during sexual
intercourse.
5. There are 8 steps for
DNA Fingerprinting
Step 1: Isolation of
DNA
DNA must be
recovered from cells
or tissue. Only a
small amount of
blood, hair, or skin is
needed to isolate
DNA
6. Step 2:Digestion of DNA by restriction
endonucleases
The DNA is cut into fragments using restriction
enzymes.
Each restriction enzyme cuts DNA at a specific
base sequence.
7. The sections of DNA that are cut out are called
restriction fragments.
This yields thousands of restriction fragments of all
different sizes because the base sequences being cut
may be far apart (long fragment) or close together
(short fragment).
Commonly used RE are (hae III, Hinf I, Alu I etc.)
Reaction mixture is incubated overnight at 37.C
8. Step 3: Electrophoretic
separation of different
fragments
Fragments are separated
on the basis of size using
a process called gel
electrophoresis.
DNA fragments are
injected into wells and an
electric current is applied
along the gel.
9. DNA is negatively
charged so it is attracted
to the positive end of
the gel.
The shorter DNA
fragments move faster
than the longer
fragments.
DNA is separated on
basis of size.
10. Step 4: Transfer DNA on
Nylon/Nitrocellulose
membrane
The DNA fragments
are transferred to a
nylon sheet by placing
the sheet on the gel and
soaking them overnight
by the process southern
blot.
11. Step5: Probing/probe
labeling
Adding radioactive or
colored probes to the
nylon sheet which is
complementary to
target sequences.
Each probe only
sticks to one or two
specific places on the
sheet.
12. Step 6: Hybridization
labeled probe DNA should be
hybridized with the
complementary sequences
located on nylon membrane for
the detection of position of later
Membrane is washed to remove
non specific binding and
clearing of the background
13. Step 7: Autoradiography
To detect the sequences in genome
bound with the hybridized
radioactive probe on membrane
Technique involves alignment of
hybridized membrane with X-ray
film
The X-ray film alter its
development shows multiple no. of
bands that looks like bar codes and
known as DNA fingerprints.
14. Step 8: Interpretation
of band patterns
Analysis of band
patterns of different
individuals
comparison of
position of bans
Computer software
are also available for
for the analysis of
DNA fingerprints.
15. Diagnosis of Inherited
Disorders
Helps diagnose disorders in
both prenatal and newborn
babies
Disorders may include
cystic fibrosis, hemophilia,
Huntington’s disease,
familial Alzheimer’s, sickle
cell anemia, thalassemia,
and much more.
16. Crime
Forensic science is the use
of scientific knowledge in
legal situations.
The DNA profile of each
individual is highly
specific.
The chances of two
people having exactly the
same DNA profile is
30,000 million to 1
(except for identical
twins).
17. The pattern of the DNA profile is then compared
with those of the victim and the suspect.
If the profile matches the suspect it provides strong
evidence that the suspect was present at the crime
scene (Note: it does not prove he committed the
crime).
If the profile doesn’t match the suspect then that
suspect may be eliminated from the enquiry.
18. A violent murder occurred.
The forensics team retrieved a blood sample from the
crime scene.
They prepared DNA profiles of the blood sample, the
victim and a suspect as follows:
20. DNA profiles can be used to determine whether a
particular person is the parent of a child.
A child’s paternity (father) and maternity(mother)
can be determined.
This information can be used in
Paternity suits
Inheritance cases
Immigration cases
21. By comparing the DNA profile of a mother and her
child
identify DNA fragments in the child which are absent
from the mother and must therefore have been
inherited from the biological father.
23. Personal Identification
The U.S. armed services
are just beginning a
program where they
collect DNA fingerprints
from all personnel for
later use, incase they
need to identify
casualties or missing
people.
24. Some other uses of DNA fingerprinting
Identification of carcass of tissues
Detection of somatic mutations or cancer
Pathogen identification
Detection of loci controlling quantitative traits or
disease resistance
Sex determination
Individual identification
25. In 2002 Elizabeth
Hurley used DNA
Fingerprinting to prove
that Steve Bing was the
father of her child
Damien
26. Colin Pitchfork was the
first criminal caught
based on DNA
fingerprinting evidence.
He was arrested in 1986
for the rape and murder
of two girls and was
sentenced in 1988.