DNA profiling and its legal applications were discussed. Key points include:
1) DNA profiling involves analyzing variable repetitive sequences in non-coding regions to obtain a unique genetic profile for identification.
2) The first use of DNA fingerprinting in a criminal case in 1986 helped exonerate a falsely accused man.
3) DNA has significant forensic applications such as identifying suspects in murder, sexual assault, and disputed parentage cases.
4) DNA analysis also has legal implications including the right to privacy versus right to information in criminal investigations.
FORENSIC DNA PROFILING: Strengths and LimitationsHezekiah Fatoki
Forensic science is defined as the application of scientific knowledge and experimentation to legal contentions, be they civil or criminal matters. DNA profiling (also called DNA typing or DNA fingerprinting) is a forensic techniques used to identify individuals by characteristics of their DNA in crime cases. DNA profiling can be use to resolve paternal and ancestral issues. This process was built mainly on the knowledge of two scientific breakthroughs. First is the Polymerase Chain Reaction (PCR) which was conceived by Kary Mullis in 1983 at Cetus Corporation, USA. Second is the Restriction Fragment Length Polymorphism (RFLP) analysis of repeated DNA sequences which was discovered by Professor Sir Alec Jeffreys in 1985 at the University of Leicester, UK. The strengths and limitations of the current and emerging forensic DNA profiling are the focus of this seminar. It is my expectation that the newly proposed synthetic human genome project will aid the strength of this process in the future.
Forensic semen analysis plays a vital role in identification of suspected person in rape cases. this slide consist of all the recquiried info. about semen and its analysis in forensic laboratories.
Parental testing is considered one of the best ways to establish a biological parent child relation between individuals
Do you believe that your partner is currently
being unfaithful?
Sample collection and preservation of biological samplesNeha Agarwal
A preliminary survey should be carried out to evaluate potential evidence. In particular, the
recognition of evidence plays a critical role in solving or prosecuting crimes. The priority of the
potential evidence at crime scenes should be assessed based on each item’s relevance to the solution
of the case. Higher priority should be assigned to evidence with probative value to the case.
For example, the evidence related to a corpus delicti is considered to be of the highest priority.
Corpus delicti is a Latin term meaning “body of crime.” In Western law, it primarily refers to the
principle that in order for an individual to be convicted, it is necessary to prove the occurrence of the crime. In a forensic investigation, it also refers to the physical evidence proving that a crime was committed
Interpretation of dna typing results and codis Neha Agarwal
An STR genotype is the allele, in the case of a homozygote, or alleles, in the
case of a heterozygote, present in a sample for a particular locus and is normally
reported as the number of repeats present in the allele. A full sample genotype
or STR profi le is produced by the combination of all of the locus genotypes into
a single series of numbers. This profi le is what is entered into a case report or
a DNA database for comparison purposes to other samples.
Confirmatory Test for Semen identificationParth Chuahan
Seminal fluid is a complex mixture of secretions from at least four male urogenital glands. The seminal vesicle gland contributes approximately 60% to this mixture, the prostate gland contributes approximately 30%, and the combined contribution of the epididymis and bulbourethral glands account for the remaining 10%
Confirmatory Tests for semen:
1- The Christmas Tree Stain: The most reliable confirmation for the presence of semen is the positive visual identification of sperm cells (or spermatozoa) using the Christmas tree stain.
this is used in crime investigators for finding the evidences where there is lack of availability of evidence. some cells that was peeled off from our any parts of body will be seen in the crime scene and it is possible to find these kind of evidence form the crime scene.
FORENSIC DNA PROFILING: Strengths and LimitationsHezekiah Fatoki
Forensic science is defined as the application of scientific knowledge and experimentation to legal contentions, be they civil or criminal matters. DNA profiling (also called DNA typing or DNA fingerprinting) is a forensic techniques used to identify individuals by characteristics of their DNA in crime cases. DNA profiling can be use to resolve paternal and ancestral issues. This process was built mainly on the knowledge of two scientific breakthroughs. First is the Polymerase Chain Reaction (PCR) which was conceived by Kary Mullis in 1983 at Cetus Corporation, USA. Second is the Restriction Fragment Length Polymorphism (RFLP) analysis of repeated DNA sequences which was discovered by Professor Sir Alec Jeffreys in 1985 at the University of Leicester, UK. The strengths and limitations of the current and emerging forensic DNA profiling are the focus of this seminar. It is my expectation that the newly proposed synthetic human genome project will aid the strength of this process in the future.
Forensic semen analysis plays a vital role in identification of suspected person in rape cases. this slide consist of all the recquiried info. about semen and its analysis in forensic laboratories.
Parental testing is considered one of the best ways to establish a biological parent child relation between individuals
Do you believe that your partner is currently
being unfaithful?
Sample collection and preservation of biological samplesNeha Agarwal
A preliminary survey should be carried out to evaluate potential evidence. In particular, the
recognition of evidence plays a critical role in solving or prosecuting crimes. The priority of the
potential evidence at crime scenes should be assessed based on each item’s relevance to the solution
of the case. Higher priority should be assigned to evidence with probative value to the case.
For example, the evidence related to a corpus delicti is considered to be of the highest priority.
Corpus delicti is a Latin term meaning “body of crime.” In Western law, it primarily refers to the
principle that in order for an individual to be convicted, it is necessary to prove the occurrence of the crime. In a forensic investigation, it also refers to the physical evidence proving that a crime was committed
Interpretation of dna typing results and codis Neha Agarwal
An STR genotype is the allele, in the case of a homozygote, or alleles, in the
case of a heterozygote, present in a sample for a particular locus and is normally
reported as the number of repeats present in the allele. A full sample genotype
or STR profi le is produced by the combination of all of the locus genotypes into
a single series of numbers. This profi le is what is entered into a case report or
a DNA database for comparison purposes to other samples.
Confirmatory Test for Semen identificationParth Chuahan
Seminal fluid is a complex mixture of secretions from at least four male urogenital glands. The seminal vesicle gland contributes approximately 60% to this mixture, the prostate gland contributes approximately 30%, and the combined contribution of the epididymis and bulbourethral glands account for the remaining 10%
Confirmatory Tests for semen:
1- The Christmas Tree Stain: The most reliable confirmation for the presence of semen is the positive visual identification of sperm cells (or spermatozoa) using the Christmas tree stain.
this is used in crime investigators for finding the evidences where there is lack of availability of evidence. some cells that was peeled off from our any parts of body will be seen in the crime scene and it is possible to find these kind of evidence form the crime scene.
Molecular diagnostic approaches which are being widely used in various fields including forensics. This presentation is about the different genetic factors which can be used as markers for crime scene investigation.
DNA Fingerprinting Explained, Techniques Used, Usage, Limitations and Contradictions.
*I won an Award for the Best Power Point Project Presentation in class 12th for this project. :D
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
1. FORENSIC SIGNIFICANCE OF DNA
PROFILING & ITS LEGAL PROSPECTIVE
NAME-AYUSHI SINGH
ID-17MSFS022
SEMESTER-3RD
2. • Dna profiling is the process where a specific
pattern called as profile is obtained from a
person. And the hypervariable regions non
coding repetitive sequences are analysed
3. HISTORY OF DNA PROFILING
• Dr. Alec J. Jeffreys in 1984 found out that there is a repeating sequences
in the DNA known as VNTRs (variable number of tandem repeats) which
can be seen as the bar code in the X-ray pictures. These sequences were
unique and even a small part of these codes was enough to determine
the identity of an individual
• In 1986, DNA fingerprinting was used in the criminal case for the first
time. At that time, Richard Buckland was accused for the rape and
murder of two young school girls. The DNA test was found negative
when the semen sample collected from the two girls did not match with
the accuse's. He was the first person to be found innocent with the help
of DNA fingerprinting
9. Murder- blood on a weapon can be matched against the blood
of the victim. blood stains on the clothing or the person of the
accused in case of murder can be matched with the blood of the
victim. Hair roots found on a weapon can be matched against
the blood of the victim and accused.
Sexual crimes- the seminal Dna obtained from the vaginal
aspirates or swab or compared with the Dna prints obtained
from blood samples of the suspect. There is no need to match
semen against semen a salivary stain or a hair transformed from
the accused to the victim can be used fr the comparison. If they
match the suspect is criminal. In the case of gang rape the y
chromosome analysis helps in tracing up the multiple
contributors. Seminal stain and vaginal epithelial cells will
identify the victim and assailant . in the case of oligospermic or
aspermia..Analysis of genetic markers on the y chromosome
through STR method
Disputed maternity Exchange of newborn in hospital or when the
two women claiming for the single child .
Botanical evidences ( pollen grain) individualization of the region
10. DNA ANCESTORY TEST
• Y chromosome (Y-DNA) test: This test only explores a man’s “patrilineal” or
direct father’s-line ancestry.
• Mitochondrial DNA test (mtDNA test)- This test tracks matrilineal or
mother’s-line heritage using the DNA in the cellular powerhouses known as
mitochondria. Whereas the Y-DNA test is for men only, an mtDNA test can
be taken by women as well.
• Autosomal DNA test- Y-DNA or mtDNA test, which only focus on one line of
your family tree, an autosomal DNA test has the power to trace all of your
family lines. Both men and women can take this test. Autosomal DNA is
contained in the 22 pairs of chromosomes not involved in determining a
person's sex. Autosomal DNA recombines each generation, and new
offspring receive one set of chromosomes from each parent. These are
inherited exactly equally from both parents and roughly equally from
grandparents to about 3x great-grand parents. Therefore, the number of
markers (one of two or more known variants in the genome at a particular
location – known as Single-nucleotide polymorphisms or SNPs) inherited
from a specific ancestor decreases by about half each generation , an
individual receives half of their markers from each parent, about a quarter
of their markers from each grandparent;
11.
12. PATERNITY DISPUTES
In this the identification of biological father ,mother and alleged father are
printed for DNA a child will have 50% dna from mother and 50% from
father the bars in the child code are first match with mother then
remaining one are match with the father. If he correspond then he is the
father. RFLP and PCR methods in .in polymerase chain reaction method
the dna is extracted, fragmented, amplified, and then separated by the
process of gel electrophoresis the dna fragments then studied and
compared. In RFLP method the extracted dna is fragmented by enzymes
and the dna fragment is sorted by size and then undergo identification.
• (PARENTAL PATERNITY TEST) is also performed .
• Buccal swabs , the result of the buccal swabs found in cheek cells are
same as the dna found in all the other test cells. Or Blood samples of both
father and mother and the child is taken. For the prior procedure
The parents would not have blood transfusion for three months before
taking the sample mutation of the genes can give rise to wrong results
13.
14. PEDIGREE ANALYSIS AND ESTABLISHING FAMILY
RELATIONSHIP
pedigree is a diagram of a family tree showing the relationships between
individuals together with relevant facts about their medical histories. A
pedigree analysis is the interpretation of these data that allows a better
understanding of the transmission of genes within the family. Usually, at
least one member of the family has a genetic disease, and by examining
the pedigree, clues to the mode of inheritance of the disorder and the
potential risk to other family members can be obtained
15. species identification and sexing
Dna barcoding helps in the identification of species The mitochondrial
cytochrome b gene sequence is analysed and the phylogentic tress of the
It is useful to know if male and female components are present in
sample the amelogenin locus will show one band in female and two
bands in male.
All the cases of biological identification
To exonerate a falsely implicated person of the crime
Diagnosis of inherited disorder in parental and new born babies . A
disease causing change in the dna of a gene is called a mutation. PCR
and DNA sequencing.. This then allows scientists to determine whether
or not there is a change or mutation present in a gene of interest.
TEST- parental testing and new born baby testing
Determining how the races have migrated one region to another it is
based on the genetic characters and phylogentetic trees identification.
16. In hit and run case accidents matching dna matching
from blood of victim from bloodstains on a vehicle.
• In the cases where the identification of the susepect is
not matched then dna profile from the crime scene
cases sent to (CODIS) to identify suspect anywhere in
the uk and link the serial killer
Identification of the mutilated bodies in cases of
accident ,bomb blast, mass attack .The dna fingerprint
obtained for such from such remains can be compared
with previous prints if available or with the close blood
relatives of the deceased ,which can establish ,links
between close relatives.
Extortion cases Saliva sample from envelope face
mask, nasal secretions, etc
17. EXHUMED CASES
Nicolas Copernicus died in 1542 shortly after completing his noted book
placing the sun at the centre of the universe. He was buried at Frombork
Cathedral in Poland, but despite his fame at the time, his tomb wasn’t
clearly marked
Archaeologists searched unsuccessfully for Copernicus for two centuries.
Finally, scans beneath a cathedral in 2005 turned up human remains that
looked like Copernicus, quite literally. The Polish Police Central Forensic
Laboratory used the skull to reconstruct a face that bears a striking
resemblance to the astronomer’s self-portrait.
Scientists also recovered DNA from the remains, but had nothing to match it
against to confirm identity; no direct descendants of Copernicus could be
found.
Eventually, a librarian found several hairs inside a book of calendars owned
by the astronomer. Genetic testing confirmed a DNA match to the remains,
• scientists look for pockets that tend to be preserved in hair, molar teeth,
and the petrous bone near the inner ear
NOTE--DNA analysis can be achieved by a combination of DNA extraction
from bone powder using a buffer consisting solely of EDTA and proteinase
K, and purification of the DNA by binding to silica in the presence of high
concentrations of guanidinium thiocyanate. All steps are performed at
room temperature (20-23 degrees C),
20. Use of DNA INFORMATION IN LEGAL
SYSTEM
• DNA USED IN CRIMINAL CASES and civil
cases are as follows
a) For individualization
b) For genealogical identification
c) Paternity test , abdoning child, concealed
birth
d) Immigration eligibility
21. SECTION 53 (crpc)An Examination of a person accused of rape by medical
practitioner authorizes a police officer to get the assistance of a medical
practitioner in good faith for the purpose of the investigation. But, it
doesn’t enable a complainant to collect blood, semen etc for bringing the
criminal charges against the accused.
SECTION53(A)- (crpc) Examination of person accused of rape by medical practioner
When a person is arrested on a charge of committing an offence of rape or an attempt
to commit rape and there are reasonable grounds for believing that an examination
of his person will afford evidence as to the commission of such offence, it shall be
lawful for a registered medical practitioner employed in a hospital run by the
Government or by a local authority and in the absence of such a practitioner within
the radius of sixteen kilometres from the place where the offence has been
committed by any other registered medical practitioner, acting at the request of a
police officer not below the rank of a sub-inspector, and for any person acting in
good faith in his aid and under his direction, to make such an examination of the
arrested person and to use such force as is reasonably necessary for that purpose.
– the name and address of the accused and of the person by whom he was
brought,
– the age of the accused,
– marks of injury, if any, on the person of the accused,
– the description of material taken from the person of the accused
for DNA profiling,
• The exact time of commencement and completion of the examination shall also be
noted in the report.
22. SEC-112 INDIAN EVIDENCE ACT
Section 112 of the Indian Evidence Act deals with the proof of legitimacy of
offspring if they are born during wedlock or within a certain period of the
280 days after its dissolution of marriage. In many ways it is a unique
section. On the one hand it establishes the fact of marriage as conclusive
proof of the legitimacy of the children and at the same time mentions that
the conclusive proof of legitimacy (i.e. marriage) can be avoided if the
parties could not have begotten the child as the spouses had no access to
each other. The obvious purpose behind such a section would be to
prevent the unnecessary bastardization of illegitimate children and the
condemning of their mothers and unchaste. However, with the advent of
DNA fingerprinting analysis some problems have arisen. DNA test that
indicated that the respondent was not the father of the child would not be
enough to rebut the conclusiveness of the marriage as proof of legitimacy
of the child. DNA analysis is of utmost importance in determining the
paternity of a child in the cases of civil disputes. Need of this evidence is
most significant in the criminal cases, civil cases, and in the maintenance
proceeding in the criminal courts
23. RIGHT TO PRIVACY VS RIGHT TO
INFORMATION
• The right to privacy is our right to keep a domain around us, which includes all those things
that are part of us, such as our body, home, property, thoughts, feelings, secrets and identity.
The right to privacy gives us the ability to choose which parts in this domain can be accessed
by others, and to control the extent, manner and timing of the use of those parts we choose
to disclose
• RIGHT TO INFORMATION
• The basic object of the Right to Information Act is to empower the citizens, promote
transparency and accountability in the working of the Government, contain corruption, and
make our democracy work for the people in real sense. It goes without saying that an
informed citizen is better equipped to keep necessary vigil on the instruments of governance
and make the government more accountable to the governed.
• DNA technology is concerned there is the conflict between two fundamental rights i.e. right
to privacy and right to information. welfare of the state and public cannot compel a person to
go for DNA or blood test because it amounts to breach of right to privacy, the courts can only
direct the parties to the criminal case or civil suits to go for the same. but it in criminal case
for the interest of the victim and welfare of then state as a part of public policy and to give
justice to victim there arise the question of right to information from the concerned person
who is suppose to disclose the relevant facts to establish the conclusive proofs during the
judicial proceedings before the courts of law.
24. KEY POINTS
• Dna testing has got legal validity in 1989 in India
kunhiraman vs. manoj was 1st paternity dispute cases
used in India
• The court is taking dna evidence as expert opinion
(section45 I.E.A) like forensic experts,balistic experts etc.
• The govt of India law commission of India the parliament
affairs board has set up an advisory committee to give
comprehensive report on dna testing
• The law commission in its 185 report has also
recommended inclusion of dna testing in the Indian
evidence act by amending section112