This document summarizes the process of fertilization in angiosperms. It describes the structures involved, including the pollen grain, which contains two cells including the generative cell that divides into two male gametes. The female gamete is contained in the embryo sac, which contains seven cells including an egg cell. Pollination involves the transfer of pollen grains to the stigma. Germination of compatible pollen on the stigma produces a pollen tube that grows down to fertilize the egg cell in a process called double fertilization, where one gamete fuses with the egg and the other with the polar nuclei. This results in the formation of a diploid zygote and triploid endosperm to nourish

1. Fertilization in an
angiosperm
A.K.JHA
P.G.T(Biology)
D.A.V. CENTENARY PUBLIC SCHOOL
SIWAN(BIHAR)

2. What is Flower?
 Flower, the reproductive portion of any plant in the division Magnoliophyta
(Angiospermae), a group commonly called flowering plants or angiosperms.
 As popularly used, the term “flower” especially applies when part or all of
the reproductive structure is distinctive in colour and form.
 Regardless of their variety, all flowers have a uniform function,
the reproduction of the species through the production of seed.
 The flower is the characteristic structure of the evolutionarily highest group
of plants, the angiosperms.

3. Structure of flower

4. Structure of pollen grain
The pollen grains represent the male
gametophytes.
It has two layered wall exine and intine.
The cytoplasm of pollen grain is surrounded by
a plasma membrane.
Mature pollen grain has two cells, the
vegetative cell and generative cell. The
vegetative cell is bigger, has abundant food
reserve and a large irregularly shaped nucleus.
The generative cell is small and floats in the
cytoplasm of the vegetative cell. It is spindle
shaped with dense cytoplasm and a nucleus.
Now pollen grains becomes bicelled and
binucleated stage. In angiosperm pollination of
pollen grains take place in bicelled and
binucleated stage in 60% Angiosperms and in 40
% angiosperms pollination occurs at 3-celled
stage. In this case generative cell divides and
form two male gametes.

5. Structure of female gametophyte
 There is cellular organization in which 3 celled
egg apparatus is formed at micropylar end and
constitute the egg appratus.The egg apparatus
consists of two synergids and one egg cell.
 Three cells are at the chalazal end and are
called the antipodals.
 The large central cell has two polar
nuclei.Thus,a typical angiosperm contains
embryo sac,at maturity though 8-nucleate is 7-
celled.

6. Pollination
 Pollination is the act of transferring pollen grains from the male anther of a
flower to the female stigma. The goal of every living organism, including
plants, is to create offspring for the next generation. One of the ways that
plants can produce offspring is by making seeds. Seeds contain the genetic
information to produce a new plant.
 Types:-Self Pollination,Cross Pollination
 Agents of Pollination:-Wind,insect, animal,mechanical explosive and water

7. Types of Pollination
 Self Pollination
It is the transfer of pollen grains from anther of a flower to the stigma of
either the same or genetically similar flower.
Accordingly,it is of two types Autogamy and geitonogamy.
 Cross Pollination
It is the transfer of pollen grains from anther of a flower to the stigma of
genetically different flower.
It is performed with the help of an external agency.

8. AGENCIES OF POLLINATION

9. Fertilization
 Fertilization is the fusion of the male(n) and
female(n) gametes to produce a zygote(2n).
 The pollen grain produces the male gametes
 Embryo sac produces an egg cell and polar nuclei

10. Germination of pollen grain on stigma
 A large number of pollen grains fall on the
stigma.
 It sets in pollen pistil interaction.
 Only the compatable pollen grains germinate
and grow. They absorb nourishment and
swell up. The swollen tube cell grows out of
the pollen grain through one of the
germpore.It produces a pollen tube.Pollen
tube is covered by intine only. Generative
cell divides into two male gametes, if it has
not divided already.

11. Path taken up by pollen tube
 Entry of pollen tube is 3 types:
 A.Porogamy
 B.Mesogamy
 C.Chalozagamy

12. Path taken up by pollen tube

13. Path taken up by pollen tube

14. Path taken up by pollen tube

15. Feritilization process
 After the discharge of the content of the pollen tube into the embryo sac one
male gamete fuses with the egg (syngamy) and the other with the two polar
nuclei (triple fusion) .Male gamete may be spherical (Erigerion) ,
ellipsoidal(Livisticum) , rod shaped (Urtica) vermiform (Lilium) in structure.
 In normal case one male gamete unite with the egg to form the Zygote and
the second sperm with the secondary nucleus. This process is called double
fertilization and also termed as triple fusion. With the result of syngamy a
zygote (2n) is formed,whereas the triple fusion results in the formation of
endosperm nucleus (3n).

16. Significance of Double fertilization in
the embryo sac
 It gives stimulus to the plant due to which ovary develops into fruit and ovules
develop into seeds.
 It restores the diploid condition by fusion of haploid male and female
gametes.
 It results in the formation of diploid zygote, which develops into an embryo
and gives rise to a new plant.
 It results in the formation of triploid primary endosperm nucleus (PEN) which
develops into endosperm in the seed. It provides nourishment to the
developing embryo.