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FERMENTATION: INDUSTRIAL ASPECTS
GULPREET KAUR
Department of Biological Sciences
Florida Institute of Technology, FL 32901
OBJECTIVES
• Describe the chemistry of Fermentation
• List at least five fermented food products
• Describe the process of industrial production of
one fermented food product
FERMENTATION?
Chemical changes in Organic substrates
through the activity of living microbes
EXAMPLES?
INDUSTRIAL ALCOHOL PRODUCTION
C6H12O6 C2H5OH + CO2
Sugar Ethanol
Yeast
Fermentation
NO OXYGEN!
Yeast
• Eukaryotic
• 3-4 µm in size
• Mostly anaerobic
• Grow on organic
compounds
Saccharomyces cerevisae
Wine Production
PROCESS OUTLINE
• Picking, handling, crushing Grapes
• Pre-treatment
• Fermentation
• Post-fermentation
• Racking
• Storage and Ageing
• Clarification
• Packing
Grape Processing
Good varieties of Grapes -
Zinfandel, Merlot, Blue
Portuguese, Refosco
Picked at Maturity –
Balling Degree of 21 to 23⁰
Crushed in Machines -
• Machine could be made of
Stainless Steel or Nickel
• Should not be made of
Iron, Steel, Tin or Copper
Pre-treatment
• Sterilization - to kill undesired microbes
• SO2 or sulphites are used
• ̴2-6 oz. Potassium Bisulphite added per tonne of
grapes
Fermentation
• Saccharomyces ellipsoideus used
• Starter culture prepared
▫ To facilitate quick growth
 Large supply of oxygen
 Stirring
▫ No alcohol produced!
• Fermentation in Tank
▫ Starter culture added to Grape Juice
▫ Time: 3-5 days
▫ Temperature: 70-90⁰F
▫ No oxygen added
▫ Stirring twice a day
▫ Initial Sugar concentration: 22 ⁰ Balling
Final concentration of alcohol = Balling reading × 0.575
At the end of this step:
• Sugar concentration : 0-4⁰ Balling
• Lots of CO2 has accumulated in the tank
Post- Fermentation Processing
• Kept in Closed Storage tanks with bungs
▫ 7-10 days
▫ Excess CO2 released
▫ No sugar left – Yeast dies off!
• Racking
• Storage and Ageing
• Packaging
QUESTIONS?
ASSESSMENT 1
• What is a starter culture? What is its purpose?
ASSESSMENT 2
• What do you think would happen if an alcohol
fermentation tank was not closed properly while
processing ?
ASSESSMENT 3
From the list given below, write an ‘F’ in front of
the foods that you think are fermented and an ‘N’
for foods that are not.
• __ Coca Cola
• __ Yoghurt
• __ Bread
• __ Hamburger
• __ Whisky
• __ Pizza

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Fermentation

  • 1. FERMENTATION: INDUSTRIAL ASPECTS GULPREET KAUR Department of Biological Sciences Florida Institute of Technology, FL 32901
  • 2. OBJECTIVES • Describe the chemistry of Fermentation • List at least five fermented food products • Describe the process of industrial production of one fermented food product
  • 3.
  • 4. FERMENTATION? Chemical changes in Organic substrates through the activity of living microbes
  • 6.
  • 7. INDUSTRIAL ALCOHOL PRODUCTION C6H12O6 C2H5OH + CO2 Sugar Ethanol Yeast Fermentation NO OXYGEN!
  • 8. Yeast • Eukaryotic • 3-4 µm in size • Mostly anaerobic • Grow on organic compounds Saccharomyces cerevisae
  • 10. PROCESS OUTLINE • Picking, handling, crushing Grapes • Pre-treatment • Fermentation • Post-fermentation • Racking • Storage and Ageing • Clarification • Packing
  • 11. Grape Processing Good varieties of Grapes - Zinfandel, Merlot, Blue Portuguese, Refosco Picked at Maturity – Balling Degree of 21 to 23⁰ Crushed in Machines - • Machine could be made of Stainless Steel or Nickel • Should not be made of Iron, Steel, Tin or Copper
  • 12. Pre-treatment • Sterilization - to kill undesired microbes • SO2 or sulphites are used • ̴2-6 oz. Potassium Bisulphite added per tonne of grapes
  • 13. Fermentation • Saccharomyces ellipsoideus used • Starter culture prepared ▫ To facilitate quick growth  Large supply of oxygen  Stirring ▫ No alcohol produced!
  • 14. • Fermentation in Tank ▫ Starter culture added to Grape Juice ▫ Time: 3-5 days ▫ Temperature: 70-90⁰F ▫ No oxygen added ▫ Stirring twice a day ▫ Initial Sugar concentration: 22 ⁰ Balling Final concentration of alcohol = Balling reading × 0.575 At the end of this step: • Sugar concentration : 0-4⁰ Balling • Lots of CO2 has accumulated in the tank
  • 15. Post- Fermentation Processing • Kept in Closed Storage tanks with bungs ▫ 7-10 days ▫ Excess CO2 released ▫ No sugar left – Yeast dies off! • Racking • Storage and Ageing • Packaging
  • 17. ASSESSMENT 1 • What is a starter culture? What is its purpose?
  • 18. ASSESSMENT 2 • What do you think would happen if an alcohol fermentation tank was not closed properly while processing ?
  • 19. ASSESSMENT 3 From the list given below, write an ‘F’ in front of the foods that you think are fermented and an ‘N’ for foods that are not. • __ Coca Cola • __ Yoghurt • __ Bread • __ Hamburger • __ Whisky • __ Pizza