Extraction is a process of separation or isolation of pharmaceutical active ingredients
from plant or animal drugs with the help of solvent.
On the basis of the physical nature of crude drug to be extracted i.e. liquid or solid ,the extraction process may be:
Liquid –Liquid Extraction Or
Solid –Liquid Extraction.
The solvent used for extraction is called as ‘Menstruum’ and the residue left after extracting desired constituents is called ‘Marc’.
Required Ideal Properties of Menstruum :
Should be inert and non –toxic
Should extract only the desirable constituent of the crude drug .
Should be cheap and easily available
Extraction is a process of separation or isolation of pharmaceutical active ingredients
from plant or animal drugs with the help of solvent.
On the basis of the physical nature of crude drug to be extracted i.e. liquid or solid ,the extraction process may be:
Liquid –Liquid Extraction Or
Solid –Liquid Extraction.
The solvent used for extraction is called as ‘Menstruum’ and the residue left after extracting desired constituents is called ‘Marc’.
Required Ideal Properties of Menstruum :
Should be inert and non –toxic
Should extract only the desirable constituent of the crude drug .
Should be cheap and easily available
This practical manual is prepared for academic purpose only and contains introduction and detail methodology regarding determination of numbers of starch grains by Lycopodium spore method, along with dummy readings to facilitate understanding of calculations thereof.
what is extraction, infusion, decoction, maceration, percolation, digestion, factors, procedure for infusion, procedure for decoction, procedure for maceration, factors for extraction
Factors affecting extraction, Pharmacognosy, crude drugs extraction factorsDivya Sree M S
Factors affecting extraction, Pharmacognosy, crude drugs extraction factors, Factors affecting choice of Extraction Process
Factors considered when selecting a solvent
This slide includes the plants containing Volatile oil, their chemical components, Structures and uses. As well as how the volatile oil is being obtained from different methods and techniques with the pictorial representation.
In that topic their is describe the different types of Extraction Methods, Parameters for Selecting appropriate Extraction methods, types of Extract, types of Separation techniques, types of distillation, chromatographic techniques.
This practical manual is prepared for academic purpose only and contains introduction and detail methodology regarding determination of numbers of starch grains by Lycopodium spore method, along with dummy readings to facilitate understanding of calculations thereof.
what is extraction, infusion, decoction, maceration, percolation, digestion, factors, procedure for infusion, procedure for decoction, procedure for maceration, factors for extraction
Factors affecting extraction, Pharmacognosy, crude drugs extraction factorsDivya Sree M S
Factors affecting extraction, Pharmacognosy, crude drugs extraction factors, Factors affecting choice of Extraction Process
Factors considered when selecting a solvent
This slide includes the plants containing Volatile oil, their chemical components, Structures and uses. As well as how the volatile oil is being obtained from different methods and techniques with the pictorial representation.
In that topic their is describe the different types of Extraction Methods, Parameters for Selecting appropriate Extraction methods, types of Extract, types of Separation techniques, types of distillation, chromatographic techniques.
This is about the large extraction, hope you all get benefit from this and feel easy to use it .
This is all about how we do extraction at large scale and what is the best procedure to do it properly, as a pharmacist we must know about little details of our formulations how they are extracted and their further process and machinery used. All the terms should be considered and handling is the most important step. This will be a ready to explain type information.
Extraction refers to processes for the isolation of the active ingredients from drug material. This may be by physical means or by dissolving in a suitable menstruum (liquid solvent eg. water or alcohol). Expression is the physical act of applying pressure to squeeze out oils or juices from plants.
All about extraction methods in pharmacognosy.
The procedure of separating active compounds, active substances, or active medications from basic materials derived either directly from plants or animals,
It is the separation of medicinally active tissues from inert or inactive components in plants or animals using specific solvents.
Solvent ;
Can be Polar or Non-polar
Depends on the nature of secondary metabolite
Example;
Polar Solvents; Water, Alcohol etc.
Non- polar; Benzene, chloroform etc.
Ideal properties of the solvent;
Must be highly selective for the compound to be extracted
Inert with the extracted compound or with other compounds in the plant material
Cost effective
Be harmless to man & eco-friendly
CHOICE OF EXTRACTION METHODS DEPENDS ON;
Size of Sample
Quantity of the extract required
Choice of solvent
The time taken for extraction
Cost
Terms used in extraction;
MENSTRUUM;
Solvent or solvent mixture used for extraction.
MISCELLA /Extract;
Solution containing extracted substances.
MARC;
Inert insoluble material that remains after extraction.
Drying of crude drugs;
To prevent microbiological contamination, it is necessary.
Drugs should be dried below 60°C unless otherwise specified.
Shade drying
Lowered heat exposure
Less chance to chemical alteration
Sun drying
Use less intense sun light
Economic, Most efficient
Far infrared drying
Less explored yet
Expensive, Used for expensive drugs
Vacuum Drying
Low Pressure rapid drying method
For thermolabile compounds
Oven/Hot air drying
Often used
Steps of Extractions;
Size reduction
Maximum surface area
Mesh size is 30-40 optimum
Extraction
Maceration, Infusion, Percolation, soxhlation etc.
Filtration
With the help of musciline cloth, filter paper, filter press
Concentration
By evaporation of solvent
Drying
Spray drying
Extraction;
Extraction is the process of efficiently dissolving & separating the desired chemical constituents from the crude drug with the use of solvent.
Types Of Extraction ;
Solid Extraction
The name refers to the separation of solid components from solid substance by using appropriate solvent. This type of extraction is generally performed before any further separation or processing..
2. Solvent Extraction
The liquid-liquid extraction is one in which phytoconstituents that are extracted by solid extraction process are partitioned between any two immiscible solvents.
Ideally this process needs to be carried out after solid extraction process & it is considered as purification process.
On a laboratory scale Solvent extraction is carried out in a separating funnel.
Mechanism of .......
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Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
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Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
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2. Definition
• Extraction is the first step to separate the desired natural
products from the raw materials.
• The process of isolation of active ingredients or active
substances or active medicaments from raw materials of
either plants or animals or directly from the natural sources
with the help of solvent is called extraction.
• In Extraction the mixture of substances is dissociated, by
dissolving each component with one or other solvents which
yields two phases – Raffinate Phase (rich in Feed Solvent)
and Extract Phase (rich in Solute)
3. General Terms
• Marc:
• The unwanted or insoluble substances left behind after the
process of extraction is called marc. These are not used for
further purpose in the process of extraction.
• Menstrum
• The solvent used in the process of extraction is called
solvent. The most commonly used menstrum is water.
4. Objectives of Extraction
• To obtain the therapeutic portion of dosage form from raw
materials and to eliminate the unwanted or inert material by
treatment with solvent.
• They play a decisive role in the determination of qualitative
and quantitative composition of extracts.
• The extracts obtained are also utilized for the isolation and
characterization of therapeutically active chemical
constituents.
6. Liquid-Liquid Extraction
• Solution of substance is brought into contact with another
solvent for the substance that is immiscible with first
solvent
• A concentration gradient is set up between the phases and
mass transfer will occur until an equilibrium is established
• As the process is controlled by mass transfer the liquid
must be thoroughly mixed to give a large enough surface
area for contact sufficient time must be allowed in the
dispersed for equilibrium to be set up
• The liquid are separated
7. Solid-Liquid Extraction
• Extraction of soluble constituents from a solid or semi-solid
by means of suitable solvent
• Examples:
• Extraction of fixed oils from seeds
• Preparation of alkaloids
• Isolation of hormones
8. Extraction Methods
• include solvent extraction, distillation method, pressing and
sublimation according to the extraction principle.
• Solvent extraction is the most widely used method.
• The extraction of drug progresses through the following
stages: (1) the solvent penetrates into the solid matrix; (2)
the solute dissolves in the solvents; (3) the solute is
diffused out of the solid matrix; (4) the extracted solutes
are collected.
9. Factors Affecting Extraction
• Any factor enhancing the diffusivity and solubility in the
above steps will facilitate the extraction.
• The properties of the extraction solvent.
• The physicochemical properties of drug.
• The particle size of the raw materials.
• The solvent-to-solid ration.
• The extraction temperature.
• The extraction duration.
10. • The physical properties which effect the drug extraction
are:
1. Gravitation 2. Diffusion 3. Osmosis 4. Adhesion
5. Capillarity 6. Convection 7. Solubility 8. Surface tension
• Wetting property of solvent can be improved by displacing
the air entrapped in the capillaries and also by using surface
active agent. Surfactant can increase the solubility by
means of solubilization.
11. • The selection of the solvent is crucial for solvent
extraction.
• Selectivity, solubility, cost and safety should be considered
in selection of solvents.
• It should also be have a low viscosity, not inflammable and
chemically and physically inert.
• Based on the law of similarity and intermiscibility (like
dissolves like), solvents with a polarity value near to the
polarity of the solute are likely to perform better and vice
versa.
12. • The main solvents used in the process of extraction are:
• 1. Water
• 2. Alcohol
• 3. Ether
• 4. Chloroform
• 5. Light petroleum
13. • Advantages Water as Solvent
i. It is very cheap
ii. It has wide solvent action
iii. It is non-toxic
iv. It is inflammable
• Disadvantages Water as Solvent
i. Microbial contamination will occur
ii. It is non-selective
iii. It promotes hydrolysis
iv. It also promotes enzymatic degradation
14. • Advantages of Alcohol
1. Has wide solvent action
2. No chance for the bacterial growth
3. It is non-toxic
4. Neutral in nature and also show compatibility with the
pharmaceutical product
5. Absorption of constituents is more than water
6. It is selective
7. No additional preservatives were required
8. Requires less heat when compared to water
• Disadvantages of Alcohol
1. It is costly
15. • Advantages of Ether
a. It is inflammable
• Disadvantages of Ether
a. It is costly
b. It has a physiological effect
c. It is unsuitable for internal administration.
16. • Advantages of Chloroform
a. it is inflammable
• Disadvantages of Chloroform
a. It is costly
b. It has a physiological effect
c. It is unsuitable for internal administration.
17. • Advantages of Light Petroleum
a. It is inflammable
• Disadvantages of Light Petroleum
a. It is costly
b. It has a physiological effect
c. It is unsuitable for internal administration
18. The Conventional Extraction
Techniques
• The Conventional Extraction Techniques
Extraction
process
infusion decoction maceration percolation digestion
Fresh infusion concentrated
Simple
maceration
Maceration
With
adjustment
Multiple
maceration
Simple
percolation
Percolation for
concentrated
preparation
Continuous
percolation
19. The Conventional Extraction Methods
1. Infusion
pouring of water over the drugs and then allowing it to
keep in contact with water for the stated period , usually 15
minutes, with occasional stirring and finally filtering off the
liquid.
• The marc is not pressed .
• The boiling water is commonly used as a solvent
20. • There are two types of infusion
• a. Fresh infusion
• Afresh infusion is an aqueous solution of active
constituents of vegetable drug prepared by the process of
infusion.
• Coarse powder of drug is used in the preparation of
infusion.
• Water used as menstrum ( because it has more penetration
power and dissolves the active constituents of the drug ).
• Pharmacopoeia states that fresh infusion should be used
with in 12 hours after its preparation because it gets
spoiled due to fungal or bacterial growth.
21. b. concentrated infusion
• Concentrated infusions are prepared by double or triple
maceration process.
• Concentrated infusions are eight times stronger than the
fresh infusion.
• Alcohol in the concentration of 20 – 25% is used as
menstrum. Hence these preparations can be stored for a
longer period due to preservative action of alcohol.
22. 2. Decoction
• In this process , the drug is boiled with water for stated
period usually10 minutes. After boiling, the liquid is
strained and water is passed through the content of the
strainer to make the required volume.
• This process is mainly used for vegetable drugs of hard and
woody nature having thermos table water soluble
constituents.
• The extract from decoction contains a large amount of
water-soluble impurities. Decoction cannot be used for the
extraction of thermolabile or volatile components.
23. 3. Maceration
• This is a very simple extraction method with the
disadvantage of long extraction time and low extraction
efficiency.
• It could be used for the extraction of thermolabile
components.
• The various types of maceration process are:
• a. simple maceration
• In this process the drug is placed with the whole of the
menstrum in a closed vessel for seven days. During this
period shaking is done occasionally. After seven days , the
liquid is strained and marc is pressed.
24. • The expressed liquid is mixed with strained liquid . It is
then filtered to make a clear liquid . The final volume is not
adjusted .
• Example: The tinctures made by simple maceration are:
• (1) Tincture of orange .
• (2) Tincture of lemon.
• b. maceration with adjustment
• A process for tinctures made from un organized drugs such
as oleo resins and gum resins . This process is known as
(maceration with adjustments), Example of the tinctures
made by maceration with adjustment are: compound
tinctures of benzoin.
25. c. multiple maceration process
process for concentrated preparations which include both
(Double Maceration ) and ( Triple Maceration )
• Multiple maceration process is carried out in the same way
as simple maceration process , but the menstrum used is
divided into two parts in double maceration process and
into three parts triple maceration process.
• Example for concentrated infusion prepared by double
maceration process: concentrated infusion of orange.
• Example for concentrated infusion are prepared by triple
maceration: Liquid Extract of Senna.
26. 4. Percolation
• Percolation is more efficient than maceration because it is a
continuous process in which the saturated solvent is
constantly being replaced by fresh solvent.
• The various percolation process used for the extraction of
drug are:-
• 4.1. simple percolation or percolation process for
tinctures:
• Apparatus (1) conical percolator
(2) cylindrical percolator
(3) steam jacketed percolator
27. • Method:
• there are three stages:
• i) Imbibition
• The powdered drug is moistened with sufficient quantity of
menstrum and allowed to stand for 4 hours in a closed
vessel.
• ii) Maceration
• the moistened drug is left in contact with menstrum for 24
hours . During this period, the menstrum dissolves the
active constituents of the drug and becomes almost
saturated with it.
• iii) percolation
• It consists of the down ward displacement of the
saturated solution formed in maceration and extraction of
the remaining active constituents present in the drug by the
slow passage of the menstrum through the column of the
drug . Example:- strong tincture of ginger.
28. • 4.2. percolation process for concentrated preparations
• percolation process for concentrated preparations are used
for preparing concentrated preparations are:
• A) reserve percolation process
• B) modified percolation process
29. • A) reserve percolation process
• In this process, generally ¾ th the volume of the finished
preparation, is reserved. Then the percolation process is
continued till the drug is completely exhausted. The
percolate is subjected to evaporation or distillation to
convert it into a soft extract. This soft extract is dissolved
in the reserve portion of percolate and then sufficient
menstrum is added to produce the required volume.
30. • 4.3. Modified percolation process
• The ratio between drug : percolate is reduced by modifying
the percolation process and hence there is a lot of saving in
heat, time and menstrum.
31. Soxhlet extraction (Continuous hot percolation process or
soxhelation)
• The Soxhlet extraction method integrates the advantages
of the reflux extraction and percolation, which utilizes the
principle of reflux and siphoning to continuously extract the
herb with fresh solvent. The Soxhlet extraction is an
automatic continuous extraction method with high
extraction efficiency that requires less time and solvent
consumption than maceration or percolation.
• The high temperature and long extraction time in the
Soxhlet extraction will increase the possibilities of thermal
degradation.
32. 5. Digestion
• The drug is extracted by heating at particular pressure.
This will increase the penetration power of the menstrum,
so that there is complete extraction of the drug.
33. 6. Reflux extraction
• Reflux extraction is more efficient than percolation or
maceration and requires less extraction time and solvent.
• It cannot be used for the extraction of thermolabile
natural products.
34. Modern or Greener
Extraction Methods
1. Pressurized liquid extraction (PLE)
• (PLE) has also been described as accelerated solvent
extraction, enhanced solvent extraction, pressurized fluid
extraction, accelerated fluid extraction, and high pressure
solvent extraction by different research groups.
• PLE applies high pressure in extraction. High pressure keeps
solvents in a liquid state above their boiling point resulting
in a high solubility and high diffusion rate of lipid solutes in
the solvent, and a high penetration of the solvent in the
matrix. PLE dramatically decreased the consumption of
extraction time and solvent and had better repeatability
compared to other methods.
35. 2. Supercritical fluid extraction (SFE)
• (SFE) uses supercritical fluid (SF) as the extraction
solvent. SF has similar solubility to liquid and similar
diffusivity to gas, and can dissolve a wide variety of natural
products. Their solvating properties dramatically changed
near their critical points due to small pressure and
temperature changes.
• Supercritical carbon dioxide (S-CO2) was widely used in
SFE because of its attractive merits such as low critical
temperature (31 °C), selectivity, inertness, low cost, non-
toxicity, and capability to extract thermally labile
compounds.
36. • The low polarity of S-CO2 makes it ideal for the extraction
of non-polar natural products such as lipid and volatile oil.
• A modifier may be added to S-CO2 to enhance its solvating
properties significantly.
37. 3. Ultrasound assisted extraction (UAE)
• Also called ultrasonic extraction or sonication, uses
ultrasonic wave energy in the extraction. Ultrasound in the
solvent producing cavitation accelerates the dissolution and
diffusion of the solute as well as the heat transfer, which
improves the extraction efficiency.
• The other advantage of UAE includes low solvent and
energy consumption, and the reduction of extraction
temperature and time.
• UAE is applicable for the extraction of thermolabile and
unstable compounds. UAE is commonly employed in the
extraction of many types of natural products
38. 4. Microwave assisted extraction (MAE)
• Microwaves generate heat by interacting with polar
compounds such as water and some organic components in
the plant matrix following the ionic conduction and dipole
rotation mechanisms. The transfers of heat and mass are in
the same direction in MAE, which generates a synergistic
effect to accelerate extraction and improve extraction
yield.
• The application of MAE provides many advantages, such as
increasing the extract yield, decreasing the thermal
degradation and selective heating of vegetal material.
39. • MAE is also regraded as a green technology because it
reduces the usage of organic solvent.
• There are two types of MAE methods: solvent-free
extraction (usually for volatile compounds), and solvent
extraction (usually for non-volatile compounds)
40. 5. Pulsed electric field (PEF) extraction
• Pulsed electric field extraction significantly increases the
extraction yield and decreased the extraction time because
it can increase mass transfer during extraction by
destroying membrane structures.
• The effectiveness of PEF treatment depends on several
parameters including field strength, specific energy input,
pulse number and treatment temperature.
• PEF extraction is a non-thermal method and minimizes the
degradation of the thermolabile compounds.
41. 6. Enzyme assisted extraction (EAE)
• The structure of the cell membrane and cell wall, micelles
formed by macromolecules such polysaccharides and
protein, and the coagulation and denaturation of proteins at
high temperatures during extraction are the main barriers
to the extraction of natural products.
• The extraction efficiency will be enhanced by EAE due to
the hydrolytic action of the enzymes on the components of
the cell wall and membrane and the macromolecules inside
the cell which facilitate the release of the natural product.
Cellulose, α-amylase and pectinase are generally employed in
EAE.
42. 7. Hydro distillation (HD) and steam distillation (SD)
Are commonly used methods for the extraction of volatile oil.
• Some natural compounds encounter decomposition in HD and
SD.