Expt 3 Bracketing bioassay
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This document describes an experiment to estimate the unknown concentration of an acetylcholine solution using a bracketing bioassay with hen's ileum tissue. The experiment involves determining the dose-response curve for a standard acetylcholine solution and then bracketing responses to the unknown solution with responses to the standard. Close bracketing of the unknown response between two standard responses allows the concentration of the unknown to be accurately calculated using the formula provided. The aim is to present the methodology for using a bracketing bioassay to estimate an unknown drug concentration.
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This document summarizes biological assays, specifically focusing on pharmaceutical quality management. It defines an assay as a procedure used to qualitatively or quantitatively assess the presence, amount, or functional activity of an analyte, such as a drug or biochemical substance. There are three main types of assays discussed: chemical assays, immunoassays, and bioassays. Bioassays are used to estimate the concentration or potency of a pharmaceutical drug by comparing its effects on animals or humans to a standard. The document outlines various techniques for bioassays including quantal, graded, and effect over time assays. It also discusses standards, accuracy, dependencies on dose and intensity, and applications of bioassays in pharmaceutical quality management.
Bioassay ,its types for theory & practical
The document discusses bioassays, which are techniques used to determine the potency or concentration of an active ingredient in a preparation. There are two main types of bioassays: quantal/direct endpoint bioassays which measure all-or-none biological responses, and graded response bioassays which measure graded responses to different doses. Graded response bioassays include methods like matching, bracketing, and interpolation bioassays which plot dose-response curves to calculate the potency of a test substance compared to a standard. Multiple point bioassays that use statistical analysis of responses to multiple doses are typically more precise and reliable.
Bioassay.pptx
This document summarizes different types and methods of bioassay. There are two main types of bioassay: qualitative and quantitative. Qualitative bioassay determines the type of biological action, while quantitative determines the amount of active constituent. Common methods include direct methods like matching and bracket methods using frog rectus muscle, and indirect methods like graphical interpolation and 3-4 point bioassays which construct dose-response curves to calculate potency ratios. The principle of bioassay is to compare a test substance to a standard preparation and determine the dose needed to produce the same biological effect.
Concept of bioassays by jayu
"The determination of the relative strength of a substance (e.g., a drug or hormone or toxicant) by comparing its effect on a test organism with that of a standard preparation." is called bioassay.
Bioassay go
The document discusses bioassay, which is the estimation of the concentration or potency of a substance by measuring its biological response over a biological system under standard conditions. It describes the purposes of bioassay such as determining drug potency and specificity. The principles and types of bioassay techniques like graded response, quantal, and multi-point assays are explained. Examples of bioassays for important drugs like insulin are provided. The document highlights the applications, uses, advantages, and disadvantages of bioassay.
Bioassay
The document discusses bioassay, which is the estimation of the concentration or potency of a substance by measuring its biological response over a biological system under standard conditions. It describes the purposes of bioassay such as determining drug potency and specificity. The principles and types of bioassay techniques like graded response, quantal, and multi-point assays are explained. Examples of bioassays for important drugs like insulin are provided. The document highlights the applications, uses, advantages, and disadvantages of bioassay.
Bioassay
Bioassay is defined as measuring the biological response of living tissues to determine the potency or concentration of an active principle in a preparation. There are various types of bioassays including quantal assays, graded assays, and multiple point assays. Bioassays can be performed on intact animals, isolated tissues, specific cells, or organisms and are useful for standardizing drugs obtained from natural sources and for measuring the activity of new or undefined substances. While powerful, bioassays can be time-consuming and expensive compared to physico-chemical methods.
Bioassays praveen tk
This document discusses bioassays, which determine the concentration, purity, or biological activity of a substance by measuring its effects on organisms, tissues, enzymes, or receptors. Bioassays are less accurate than chemical assays but are necessary when the active substance is unknown, isolation is not possible, or the sample quantity is too small. The principles of bioassays involve comparing the pharmacological response of an unknown sample to a reference standard using an appropriate pharmacological model and statistical analysis. Common types of bioassays include quantal, graded, matching, interpolation, bracketing, and multipoint assays which employ techniques like Latin square designs to reduce errors.
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Expt 2- To determine anti-allergic activity by mast cell stabilization assay.
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Expt 3 Bracketing bioassay
- 1. EXPERIMENT NO: 03 AIM : To estimate unknown concentration of acetylcholine solution by bracketing bioassays using hen’s ileum. Presented by: Prof. Mirza Anwar Baig Dept of Pharmcaology AIKTC, School of Pharmacy, New Panvel
- 2. REQUIREMENTS: 1. Basal tension – 0.5 gm 2. Extra load- 1 gm 3. Animal – Hen’s ileum tissue 4. Drug – Acetylcholine stock solution (10 μg/ml) & test solution. 5. Physiological solution – Tyrode solution 6. Contact time – 90sec 7. Magnification – 5-7 times.
- 3. PRINCIPLE:- o Matching & bracketing method is very useful if the amount of material is small and if the sensitivity of the tissue is not steady. o The results of the assay do not depend on the actual measurement of response. o It is decided whether a particular concentration of test solution is greater or smaller than that produced by the dose of the standard
- 4. Bioassay by Bracketing Method Performed by selecting two standard doses, which will give a close bracket on either side of the response produced by the unknown. The working dose of standard is first determined in the sensitive part of dose-response curve, that is, a dose that will approximately produce 50% of the maximal concentration. The dose of the standard drug is kept constant throughout the experiment, in order to have some idea about the change in the sensitivity of tissue with time. The standard drug is added at fixed intervals but alternating with the test so that each response produced by a dose of test substance is bracketed by responses produced by the dose of standard. Close bracketing gives more accurate results.
- 5. Observation on kymograph paper:
- 6. Formula to calculate concentration of unknown drug:
- 8. Result:
- 9. References: 1. Kulkarni S. K. Handbook of Experimental Pharmacology, Vallabh Prakashan, New Delhi. 2. Ghosh M.N. Fundamentals of Experimental Pharmacology Hilton & Company, Kolkata
- 10. Thank You