The document describes an experiment evaluating the diuretic activity of three drugs - furosemide, torsemide, and metolazone - in rats. Rats were divided into groups and administered one of the drugs along with saline. Urine output was measured over 3 hours. Torsemide showed the highest diuretic activity, followed by furosemide, with metolazone showing the lowest activity. Statistical analysis confirmed torsemide, furosemide, and metolazone all significantly increased urine output compared to the control group.
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 6 Study of effect of drugs on gastrointestinal motilityVISHALJADHAV100
Objective
Principle
Requirements
Preparation of Tyrode solution
Procedure
Kymograph recording of contractions
Observation table
Result and Interpretation
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 6 Study of effect of drugs on gastrointestinal motilityVISHALJADHAV100
Objective
Principle
Requirements
Preparation of Tyrode solution
Procedure
Kymograph recording of contractions
Observation table
Result and Interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 4 DRC of acetylcholine using frog rectus abdominis muscleVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 2 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
FIRST ESTABLISHED IN 1999
FIRST CORPORATE HOSPITAL IN GUJARAT, SUCCESSFULLY RENDERING SERVICES FOR LAST ELEVEN YEARS
MULTISPECIALITY CLINICS IN ONE ROOF
IT HAS SERVED LARGE NUMBER OF PATIENTS OF GUJARAT, RAJASTHAN AND MADYAPRADESH STATE AND PROVIDED SUITABLE PLATFORM FOR DOCTORS TO WORK IN HARMONY
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 4 DRC of acetylcholine using frog rectus abdominis muscleVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 2 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
FIRST ESTABLISHED IN 1999
FIRST CORPORATE HOSPITAL IN GUJARAT, SUCCESSFULLY RENDERING SERVICES FOR LAST ELEVEN YEARS
MULTISPECIALITY CLINICS IN ONE ROOF
IT HAS SERVED LARGE NUMBER OF PATIENTS OF GUJARAT, RAJASTHAN AND MADYAPRADESH STATE AND PROVIDED SUITABLE PLATFORM FOR DOCTORS TO WORK IN HARMONY
Drugs used in urinary inconsistancy or scanty of urine, that promote urine formation and increases urine output are explained in the ppt by Dr. Mrunal Akre
Formulation and Evaluation of Unidirection Bucco- Adhesive Tablet of Sumatrip...Ajay Champaneri
The objective of this research work was to formulate and evaluate PEO WSR
301 bucco-adhesive tablet in combination with Carbopol 934p for controlled
release of Sumatriptan Succinate. To bypass high hepatic first pass metabolism,
unidirection bucco-adhesive tablet is selected dosage form for the experimental
work. Initially preliminary trials were carried out for the selection of excipients
and their relative quantity for incorporation in the dosage form. From the results,
Polyethylene oxide-PEO WSR 301 (mucoadhesive polymer) and Carbopol 934p
(control release) were selected as a suitable excipients for experimentation.
Composition of the mucoadhesive tablet was optimized using 32 full factorial
design where amount of PEO WSR 301 (X1) and amount of Carbopol 934p (X2)
were taken as independent variables and mucoadhesive strength, Drug release
at 6 hour and % swelling index taken as response variables. The formulations of
design batches were characterized for post compression parameters like weight
variation, hardness, thickness, friability, Drug content, swelling index, ex-vivo
Mucoadhesive strength, and surface pH, drug release at 6 hr., ex-vivo residence
time, and curve fitting analysis. The optimized formulation was obtained using
Minitab software based on desirability value. Characterization of optimized
batch was carried out by, ex-vivo permeation study.
this case study describes about maxillofacial trauma , which details about the treatment, management , diagnosis, surgical options, patient counselling, pharmacist interventions & discussions are followed in this case .
please comment
thank u
martinsuja369@gmail.com
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
for beginners, providing thorough training in areas such as SEO, digital communication marketing, and PPC training in Noida. After finishing the program, students receive the certifications recognised by top different universitie, setting a strong foundation for a successful career in digital marketing.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Executive Directors Chat Leveraging AI for Diversity, Equity, and InclusionTechSoup
Let’s explore the intersection of technology and equity in the final session of our DEI series. Discover how AI tools, like ChatGPT, can be used to support and enhance your nonprofit's DEI initiatives. Participants will gain insights into practical AI applications and get tips for leveraging technology to advance their DEI goals.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
1. Expt No- 3 Evaluation of diuretic activity I Sem M.Ph 27-8-2019, PESCP, PESU Page 1
Exp No- 3 Date- 27-8-2019
Evaluation of diuretic activity
Aim: To evaluate the effect of diuretics (frusemide, torsemide and metolazone) on the
output of urine in rats.
Requirements: Rats, metabolic cages, graduated measuring cylinder, oral feeding
cannula.
Drugs and solutions: Normal saline, frusemide, torsemide, metolazone.
Theory: Diuretics are compounds which increase the flow of urine. Loop diuretics act on
the Na+-K+-2Cl− symporter (co-transporter) in the thick ascending limb of the loop of
Henley to inhibit sodium, chloride and potassium reabsorption. Furosemide or
frusemide (Lasix) is a potent loop diuretic used in the treatment of oedematous states
associated with cardiac, renal and hepatic failure, and for the treatment of
hypertension. Torsemide is another potent loop diuretic.
The members of thiazide class of diuretics are derived from benzothiadiazine. They
control hypertension in part by inhibiting reabsorption of sodium (Na+) and chloride (Cl−)
ions from the distal convoluted tubules in the kidneys by blocking the thiazide-sensitive
Na+-Cl− symporter. E.g. Hydrochlorthiazide, metolazone.
Normal urine output in rats is very small (1-2ml/rat/day). Hence to get measurable
quantity the animals are first hydrated. The urine output is increased after the
administration of diuretics like urea and frusemide, etc. Increase in volume of urine is
measured with the help of measuring cylinder and compared with the normal urine
output.
Procedure- Albino rats (150-200g) are fasted (deprived of food and water) overnight
and normal saline (25ml/kg po) was administered orally with the help of oral gavaging
needle. These were divided into four groups of 6 animals each. To the group I only
normal saline was administered. To the group II along with normal saline, furosemide
(10mg/kg ip) administered parenterally. To the group III along with normal saline,
torsemide (10mg/kg po) was also administered. To the group IV along with normal
saline, metolazone (2mg/kg ip) administered. After the administration of drugs, animals
2. Expt No- 3 Evaluation of diuretic activity I Sem M.Ph 27-8-2019, PESCP, PESU Page 2
were placed in four different metabolic cages. Urine was collected in a measuring
cylinder. Time, when the first drop of urine was collected in a cylinder for each group is
noted down and the volume was recorded at intervals of 30 min for 3 h.
Observation table:
Preparation of drug solutions-
1.Normal saline- 900 mg NaCl dissolved in 100 ml water
2.Frusemide- Each ampoule contains- 10 mg/ml. stock solution-10mg/ml
3.Torsemide- Each tab contains-10mg torsemide. stock solution- 2 tab dissolved in 20 ml
water- 10mg/ml.
4. Metolazone- Each tab contains- 2.5 mg metolazone. Stock solution- Each tab
dissolved in 2.5 ml water- 1mg/ml solution.
Group I (NS) Dose- 25ml/kg BW po
SN Markings Wt in
gm
NS
in ml
Collected urine in ml
30min 60min 90min 120min
1 H 137 3.42 0.2 0.5 0.7 0.9
2 B 137 3.42 0.3 0.5 0.8 1.0
3 HB 138 3.45 0.2 0.4 0.7 0.8
4 TT 140 3.50 0.1 0.3 0.6 0.9
5 TB 141 3.52 0.2 0.5 0.7 1.0
6 UM 139 3.47 0.3 0.5 0.8 1.0
SD±MEAN 0.21±0.075 0.45±0.083 0.71±0.075 0.93±0.081
3. Expt No- 3 Evaluation of diuretic activity I Sem M.Ph 27-8-2019, PESCP, PESU Page 3
Group II (NS) Dose- 25ml/kg po; Furosemide (10mg/kg bw ip) Stock sol 10 mg/ml
(Ampoule -> 20mg/2ml)
SN Markings Wt in
gm
NS in
ml
Furosemide Collected urine in ml
mg Ml 30min 60min 90min 120min
1 H 167 4.17 1.67 0.16 1.6 3.8 4.6 4.8
2 B 120 3.00 1.20 0.12 1.5 3.2 4.2 4.2
3 HB 145 3.62 1.45 0.14 1.5 3.7 4.5 4.8
4 TT 138 3.45 1.38 0.13 1.5 3.3 4.4 4.6
5 TB 150 3.75 1.50 0.15 1.5 3.4 4.2 4.7
6 UM 160 4.00 1.60 0.16 1.3 3.2 4.3 4.2
SD±MEAN 1.48±0.098 3.43±0.258 4.36±0.163 4.55±0.28
Group III (NS) Dose- 25ml/kg po + Torsemide (10mg/kg bw po) Stock Soln. Stock-
10mg/ml Each tab- 10mg (2 tab dissolved in 2ml)
SN Markings Wt gm NS
ml
Torsemide Collected urine in ml
mg Ml 30min 60min 90min 120min
1 H 129 3.22 1.29 0.13 4.4 7.5 8.8 9.2
2 B 125 3.12 1.25 0.12 4.2 6.5 8.2 8.4
3 HB 130 3.25 1.30 0.13 4.3 7.4 8.7 9.1
4 TT 126 3.15 1.26 0.12 4.2 6.6 8.3 8.5
5 TB 130 3.25 1.30 0.13 4.3 7.4 8.4 8.6
6 UM 140 3.50 1.40 0.14 4.2 6.6 8.5 8.7
SD±MEAN 4.26±0.081 7±0.477 8.48±0.231 8.75±0.327
4. Expt No- 3 Evaluation of diuretic activity I Sem M.Ph 27-8-2019, PESCP, PESU Page 4
Group IV (NS) Dose- 25ml/kg po + Metolazone (2mg/kg bw ip) Stock soln: 1 mg/ml
Each tab- 2.5 mg (one tab dissolved in 2.5 ml 1 mg/ml)
SN Markings Wt
gm
NS
ml
Metolazone Collected urine in ml
mg ml 30min 60min 90min 120min
1 H 131 3.27 0.26 0.26 0.4 1.2 1.3 1.5
2 B 140 3.50 0.28 0.28 0.6 1.4 1.5 1.7
3 HB 135 3.25 0.27 0.27 0.4 1.3 1.3 1.6
4 TT 146 3.65 0.29 0.29 0.5 1.3 1.3 1.5
5 TB 140 3.50 0.28 0.28 0.6 1.5 1.4 1.7
6 UM 135 3.37 0.27 0.27 0.5 1.4 1.5 1.6
SD±MEAN 0.5±0.089 1.35±0.104 1.38±0.098 1.6±0.089
Statistical analysis-
Statistical Analysis (One way ANOVA followed by Dunnett compare all column
versus control column) Collected urine in ml after 30 min.
Dunnett's Multiple
Comparison Test Mean Diff. q
Significant? P <
0.05? Summary 95% CI of diff
Normal vehicle vs
Frusemide -1.267 25.33 Yes *** -1.394 to -1.140
Normal vehicle vs
Torsemide -4.050 81.00 Yes *** -4.177 to -3.923
Normal vehicle vs
Metolazone -0.2833 5.667 Yes *** -0.4104 to -0.1563
30
M
in
0
1
2
3
4
5
Normal vehicle
Frusemide
Torsemide
Metolazone
MINUTES
collectedurineinml
5. Expt No- 3 Evaluation of diuretic activity I Sem M.Ph 27-8-2019, PESCP, PESU Page 5
Statistical Analysis (One way ANOVA followed by Dunnett compare all column
versus control column) Collected urine in ml after 60 min.
Dunnett's Multiple
Comparison Test Mean Diff. q Significant? P < 0.05? Summary 95% CI of diff
Normal vehicle vs
Frusemide -2.983 18.48 Yes *** -3.393 to -2.573
Normal vehicle vs
Torsemide -6.550 40.58 Yes *** -6.960 to -6.140
Normal vehicle vs
Metolazone -0.9000 5.576 Yes *** -1.310 to -0.4899
60
M
in
0
2
4
6
8
Normal vehicle
Frusemide
Torsemide
Metolazone
MINUTES
collectedurineinml
Statistical Analysis (One way ANOVA followed by Dunnett compare all column
versus control column) Collected urine in ml after 90 min.
Dunnett's Multiple
Comparison Test Mean Diff. q Significant? P < 0.05? Summary 95% CI of diff
Normal vehicle vs
Frusemide -3.650 40.88 Yes *** -3.877 to -3.423
Normal vehicle vs
Torsemide -7.767 86.99 Yes *** -7.993 to -7.540
Normal vehicle vs
Metolazone -0.6667 7.467 Yes *** -0.8935 to -0.4398
6. Expt No- 3 Evaluation of diuretic activity I Sem M.Ph 27-8-2019, PESCP, PESU Page 6
90
M
in
0
2
4
6
8
10
Normal vehicle
Frusemide
Torsemide
Metolazone
MINUTES
collectedurineinml
Statistical Analysis (One way ANOVA followed by Dunnett compare all column
versus control column) Collected urine in ml after 120 min.
Dunnett's Multiple
Comparison Test Mean Diff. q Significant? P < 0.05? Summary 95% CI of diff
Normal vehicle vs
Frusemide -3.617 27.97 Yes *** -3.945 to -3.288
Normal vehicle vs
Torsemide -7.817 60.45 Yes *** -8.145 to -7.488
Normal vehicle vs
Metolazone -0.6667 5.155 Yes *** -0.9952 to -0.3382
120
M
in
0
2
4
6
8
10
Normal vehicle
Frusemide
Torsemide
Metolazone
MINUTES
collectedurineinml
7. Expt No- 3 Evaluation of diuretic activity I Sem M.Ph 27-8-2019, PESCP, PESU Page 7
Gp Group Name Collected urine in ml Diuretic
IndexAfter 30min After 60min After 90min After 120min
I Normal 0.21±0.075 0.45±0.083 0.71±0.075 0.93±0.081 _____
II Furosemide 1.48±0.098*** 3.43±0.258*** 4.36±0.163*** 4.55±0.28*** 4.89±3.45
III Torsemide 4.26±0.081*** 7±0.477*** 8.48±0.231*** 8.75±0.327*** 9.40±4.03
IV Metolazone 0.5±0.089*** 1.35±0.104*** 1.38±0.098*** 1.6±0.089*** 1.72±1.09
Results
30
M
in
60
M
in
90
M
in
120
M
in
0
2
4
6
8
10
Normal vehicle
Frusemide
Torsemide
Metolazone
MINUTES
collectedurineinml
Results and Discussion:
Furosemide is a loop diuretic which acts by inhibiting the luminal Na+-K+-2Cl− symporter
(co-transporter) in the thick ascending limb in the loop of Henle, by binding to the
chloride transport channel, thus causing sodium, chloride, and potassium loss in urine.
Torsemide is a potent loop diuretic which acts by the same mechanism as that of
furosemide. Metolazone belongs to the class of thiazide diuretics which acts by
inhibiting the Na+-Cl− symporter in the distal convoluted tubule of the kidney.
On comparing the results of all the three diuretics, it was found that Torsemide shown
highest diuretic activity whose efficacy is followed by that of Furosemide and this is
followed by Metolazone which has shown the least diuretic activity among the three
drugs.