For many years rodent telemetry researchers have struggled with limited data collection flexibility, challenges of scalability and a combination of high setup and operation costs that have combined to limit research applications, leaving significant preclinical findings undiscovered. Implant communication interference has demanded special install conditions, systems were too bulky and implants were constantly being explanted and returned for service. This webinar presents new telemetry technologies that address these challenges and introduce exciting capabilities to preclinical researchers working with rodent models. In this exclusive webinar sponsored by Indus Instruments, Dr. Anil Reddy and Graham Sattler will discuss new research possibilities and advantages that a recently released telemetry system brings to mouse, rat and other small animal researchers. Attendees will see novel data collection protocols and learn how long-term monitoring, device recovery and reuse, study scalability and social housing are enabled with the MouseMonitor Telemetry system. Key Topics: data collection flexibility through customizable scheduling the importance of device reusability the research impact of long-term monitoring studies simplified system setup and operation the importance of social housing on animal welfare compact, mobile system installations with greatly reduced vertical & horizontal cage spacing

1. Examining New Research Capabilities
and Technology for Preclinical
Telemetry in Rodents
Sponsored by:
Anil K. Reddy, PhD
Baylor College of Medicine
& Indus Instruments
Nick Glover
InsideScientific
Graham Sattler
Indus Instruments

2. InsideScientific is an online educational environment
designed for life science researchers. Our goal is to aid in
the sharing and distribution of scientific information
regarding innovative technologies, protocols, research
tools and laboratory services.

3. Lifetime, Continuous Telemetric Monitoring
in Mice and Rats
MouseMonitor™ Telemetry • No more PC reboot data loss
- Implants can store data in internal
memory
• Collect data anywhere, even in the
vivarium
- Implants work on treadmills,
rotarods & mazes away from PCs
• Collect ECG continuously for more
than a year
• Wireless charging enables true long-
term data collection

4. Examining New Research
Capabilities and Technology for
Preclinical Telemetry in Rodents
Anil K. Reddy, PhD
Assistant Professor,
Medicine-Cardiovascular Sciences,
Baylor College of Medicine
Senior Scientist
Indus Instruments

5. Telemetry: Usage Expands in Preclinical Research
Significant Growth in Rodent
Telemetry Publications
• ~1100% in mice
• ~400% in rats
Source: Pubmed
& → Number of Publications
→ Percent Increase of Publications
Some Applications Not Suited
for Telemetry until Now
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6. Telemetry: New Capabilities & Possibilities
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Biotelemetry
in rats & mice
in a metal
cage or box
in a maze on a rotarod on a treadmill
on a wheel
in a water
challenge
Next: A review of some challenges facing researchers
using existing telemetry systems

7. Telemetry: Challenges
3
Challenges with existing
telemetry systems…
• Unable to collect long-term data due to finite battery life
• Manual power management
• Solitary animal housing
• Charging & communication design requires wide spacing
and shielding
• Poor system scaling for large studies

8. Challenge 1: Long-Term Data Collection
Limited Data Collection Flexibility:
• Short implant battery life
• Require continuous PC operation
• Generate massive quantities of data
MouseMonitor Telemetry
Enabling Technologies:
• Memory inside implant
• Wireless charging of implant rechargeable battery
• Powerful scheduled recording capabilities
4

9. Cumbersome & Limited Power Management:
• Fixed battery life (2-6 months)
• Require manual power switching to conserve power
• No automated low power mode
• Limited data collection scheduling capability
Challenge 2: Power Management
5
MouseMonitor Telemetry
Enabling Technologies:
• Flexible recording & scheduling capabilities
• Low power mode with automatic power optimization
• Ultra low power electronics
• Wireless charging of implant rechargeable battery

10. Challenge 3: Solitary Animal Housing
Implanted animals must be housed singly, leading to…
• Negative impacts of social isolation
• Wide cage spacing means fewer visual cues
• No group grooming or huddling behavior
• Metallic cage barriers mean fewer visual cues
Social housing means better
surgical recovery and outcomes
6
MouseMonitor Telemetry
Enabling Technologies:
• Digital communications & unique implant IDs
• Multiple animals per cage implant charging
Works with multiple animals per cage
No communication cross-talk or need for metallic barriers

11. Challenge 4: Significant Space Requirements
7
System Needs Large Installation Space:
• One communicating base needed for each implant
• Wide cage spacing means fewer visual cues
• Multiple layers of hardware data aggregation
• Metallic cage barriers mean fewer visual cues
MouseMonitor Telemetry
Enabling Technologies:
• Simple communications setup; one Communication
Module & PC for up to 100 implants
• Digital communications & unique implant IDs
• Multiple animals per cage implant charging
8 implant system installed on a custom
mobile lab cart in 18” x 48” x 54” space

12. Solutions needed:
1. Rechargeable battery
– long-term data over animal life span
2. Improved power management
– recording scheduling & low power mode
3. New approach to data acquisition
4. Communication & charging technologies
that allow animals to be socially housed
8
Telemetry: Challenges & Solutions
Challenges with existing
telemetry systems…
• Unable to collect long-term data
due to finite battery life
• Manual power management
• Solitary animal housing
• Charging & communication design
requires wide spacing and shielding
• Poor system scaling for large studies

13. System Overview: In Vivo Measurements
Heart RateElectrocardiogramTemperature
… and more capabilities coming in 2016
Activity
9

14. System Overview:
System Setup
• Communication Module
works with up to 100
implants
• Chargers works with
multiple animals per cage
• PC Configuration software
makes it easy to find
study data
10

15. • Data Recorded to
Implant Internal Memory
 Records while PC off or
PC restarting
 Can store 2 weeks of
continuous ECG data
 Can store months of
temperature & activity
data
• Data Transmitted to PC
When in Range
System Overview:
Data Handling
11

16. System Overview: Configuration Software
12

17. A) Continuous Data
• Continuous 24/7 ECG
• Periodic Temperature, Heart
Rate & Activity
B) Periodic Data
• Scheduled Captures of ECG
• Periodic Temperature, Heart
Rate & Activity
Data Acquisition Scheduling & Flexibility
13

18. MouseMonitor™ Telemetry
Continuous Data
14

19. Continuous Data: 10 week Zoom View
15

20. Continuous Data: 1 minute Zoom View
16

21. Continuous Data: 10 second Zoom View
17

22. Continuous Data: 1 second Zoom View
18

23. MouseMonitor™ Telemetry
Periodic Data
19

24. Periodic Data: 1 week Zoom View
20

25. Periodic Data: 1 week Zoom View
21

26. Periodic Data: 1 week Zoom View
22

27. Periodic Data: 10 second Zoom View
23

28. Periodic Data: 1 second Zoom View
24

29. Indus Logo?
Easy to Setup
and Use
25
• Unique implant IDs make
it easy to quickly find the
data for a specific animal
• Implants automatically
charge when in range of a
charger
• Ask about our custom
mobile lab cart solutions
for a compact system that
fits in small spaces

30. MouseMonitor Telemetry™ Advantages
• Designed to enable new applications
• Installs in a compact space
• Enables long-term data collection
• Allows socially housed subjects
• Schedule recordings with both
continuous & periodic data
• Easy to setup & use
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31. Downloadable Online Resources
MouseMonitor Telemetry Downloads: (www.mousemonitor.com/telemetry/downloads)
Downloads
• Overview Flyer
• Sample IACUC
Protocol
• Quickstart Guide
• Sterilization
Procedure
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32. Resources & Contact Info
MouseMonitor Telemetry Website: (www.mousemonitor.com/telemetry)
Contact Information
Graham Sattler
Product Development Manager
gsattler@indusinstruments.com
Phone: 281-286-1130
Dr. Anil K. Reddy
Assistant Professor of Medicine
Cardiovascular Sciences,
Baylor College of Medicine
areddy@bcm.edu
Phone: 713-798-6831
Senior Scientist
areddy@indusinstruments.com
Phone: 281-286-1130
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33. Thank You!
For additional information on the MouseMonitor™
Telemetry system and additional solutions for
preclinical research please visit:
http://www.indusinstruments.com