ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used technique for detecting antigens or antibodies. It involves coating a microtiter plate with an antigen or antibody and detecting its binding with an enzyme-linked secondary antibody. There are several types of ELISA including direct, indirect, sandwich, and competitive ELISA, which differ in their detection methods and targets. ELISA has many applications, such as detecting antibodies for diseases like tuberculosis, hepatitis B, and HIV, as well as detecting food allergens and bacterial toxins.