Electron spin resonance (ESR) spectroscopy detects transitions between spin energy levels of unpaired electrons using microwave radiation. When an unpaired electron is near a nucleus with non-zero spin, the electron experiences a magnetic field from the nucleus that splits the ESR signal into multiple lines based on the nuclear spin. This splitting is called hyperfine coupling and provides information about electronic structure. Superhyperfine splitting occurs when the electron interacts with multiple equivalent nuclei and results in even finer splitting patterns. Anisotropic interactions like the g-tensor can also be observed in ESR and provide information about electronic environments.
For UG students of All Engineering Branches (Mechanical Engg., Chemical Engg., Instrumentation Engg., Food Technology) and PG students of Chemistry, Physics, Biochemistry, Pharmacy
The link of the video lecture at YouTube is
https://www.youtube.com/watch?v=t3QDG8ZIX-8
NQR - DEFINITION - ELECTRIC FIELD GRADIENT - NUCLEAR QUADRUPOLE MOMENT - NUCLEAR QUADRUPOLE COUPLING CONSTANT - PRINCIPLE OF NQR - ENERGY OF INTERACTION - SELECTION RULE - FREQUENCY OF TRANSITION - APPLICATIONS
Photoelectron spectroscopy
- a single photon in/ electron out process
• X-ray Photoelectron Spectroscopy (XPS)
- using soft x-ray (200-2000 eV) radiation to
examine core-levels.
• Ultraviolet Photoelectron Spectroscopy (UPS)
- using vacuum UV (10-45 eV) radiation to
examine valence levels.
For UG students of All Engineering Branches (Mechanical Engg., Chemical Engg., Instrumentation Engg., Food Technology) and PG students of Chemistry, Physics, Biochemistry, Pharmacy
The link of the video lecture at YouTube is
https://www.youtube.com/watch?v=t3QDG8ZIX-8
NQR - DEFINITION - ELECTRIC FIELD GRADIENT - NUCLEAR QUADRUPOLE MOMENT - NUCLEAR QUADRUPOLE COUPLING CONSTANT - PRINCIPLE OF NQR - ENERGY OF INTERACTION - SELECTION RULE - FREQUENCY OF TRANSITION - APPLICATIONS
Photoelectron spectroscopy
- a single photon in/ electron out process
• X-ray Photoelectron Spectroscopy (XPS)
- using soft x-ray (200-2000 eV) radiation to
examine core-levels.
• Ultraviolet Photoelectron Spectroscopy (UPS)
- using vacuum UV (10-45 eV) radiation to
examine valence levels.
It contains the basic principle of Mossbauer Spectroscopy.
Recoil energy, Dopler shift.
The instrumentation of Mossbauer Spectroscopy.
Hyperfine interactions.
A ppt compiled by Yaseen Aziz Wani pursuing M.Sc Chemistry at University of Kashmir, J&K, India and Naveed Bashir Dar, a student of electrical engg. at NIT Srinagar.
Warm regards to Munnazir Bashir also for providing us with refreshing tea while we were compiling ppt.
Electron Spin Resonance (ESR) SpectroscopyHaris Saleem
Electron Spin Resonance Spectroscopy
Also called EPR Spectroscopy
Electron Paramagnetic Resonance Spectroscopy
Non-destructive technique
Applications
Extensively used in transition metal complexes
Deviated geometries in crystals
This presentation describes about the preparation, properties, bonding modes, classification and applications of metal Dioxygen Complexes. Also explains the MO diagram of molecular oxygen.
An overview of the use of the Marcus Theory to calculate the energies of transition states.
Contributed by: Elizabeth Greenhalgh, Amanda Bischoff, and Matthew Sigman, University of Utah, 2015
It contains the basic principle of Mossbauer Spectroscopy.
Recoil energy, Dopler shift.
The instrumentation of Mossbauer Spectroscopy.
Hyperfine interactions.
A ppt compiled by Yaseen Aziz Wani pursuing M.Sc Chemistry at University of Kashmir, J&K, India and Naveed Bashir Dar, a student of electrical engg. at NIT Srinagar.
Warm regards to Munnazir Bashir also for providing us with refreshing tea while we were compiling ppt.
Electron Spin Resonance (ESR) SpectroscopyHaris Saleem
Electron Spin Resonance Spectroscopy
Also called EPR Spectroscopy
Electron Paramagnetic Resonance Spectroscopy
Non-destructive technique
Applications
Extensively used in transition metal complexes
Deviated geometries in crystals
This presentation describes about the preparation, properties, bonding modes, classification and applications of metal Dioxygen Complexes. Also explains the MO diagram of molecular oxygen.
An overview of the use of the Marcus Theory to calculate the energies of transition states.
Contributed by: Elizabeth Greenhalgh, Amanda Bischoff, and Matthew Sigman, University of Utah, 2015
NMR SPECTROSCOPY ,Relaxation,longitudinal / spin- spin relaxation,transverse / spin- spin relaxation,Shielding of proton ,Deshielding of proton,CHEMICAL SHIFT,Factors Influencing Chemical Shift,Inductive effect, Vander Waal’s deshielding,Anisotropic effect (space effect),Hydrogen bonding
,SPLITTING OF THE SIGNALS,COUPLING CONSTANT,NMR SIGNAL IN VARIOUS COMPOUND
STRUCTURE OF ATOM
Sub atomic Particles
Atomic Models
Atomic spectrum of hydrogen atom:
Photoelectric effect
Planck’s quantum theory
Heisenberg’s uncertainty principle
Quantum Numbers
Rules for filling of electrons in various orbitals
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
3. Principles of EMR spectroscopy
B 0
∆E
hν
Classical theory:
Electron spin moment interacts with
applied electromagnetic radiation
m s = —
1
2
m s
= —
1
2
-
Energy
Quantum theory:
transitions between energy levels
induced by magnetic field
Resonance condition
hν = gµBB0
4.
5. The EPR experiment
• Put sample into
experimental
magnetic field (B)
• Irradiate
(microwave
frequencies)
• Measure
absorbance of
radiation as f(B) Weil, Bolton, and Wertz, 1994, “Electron Paramagnetic Resonance”
6. The hyperfine effect
• The magnetic field experienced by the unpaired electron
is affected by nearby nuclei with non-zero nuclear spin
Weil, Bolton, and Wertz, 1994, “Electron Paramagnetic Resonance”, New York: Wiley Interscience.
7. Hyperfine splitting of EPR spectra
• The magnitude of the splitting and the
number of lines depend upon:
– The nuclear spin of the interacting nucleus
• # of lines = 2n(I + ½) so I = ½ gives 2 lines, etc.
– The nuclear gyromagnetic ratio
– The magnitude of the interaction between the
electronic spin and the nuclear spin
• Magnitude of the splitting typically decreases
greatly with increasing numbers of bonds between
the nucleus and unpaired electron
8. 10 Gauss
No hyperfine
1H)
14N)
2 identical I=1/2 nuclei
1 I=5/2 nucleus (17O)
Hyperfine coupling
If the electron is surrounded by n spin-
active nuclei with a spin quantum
number of I, then a (2nI+1) line pattern
will be observed in a similar way to
NMR.
In the case of the hydrogen atom (I= ½),
this would be 2(1)(½) + 1 = 2 lines.
9. Some nuclei with spins
Element Isotope Nuclear No of %
spin lines abundance
Hydrogen 1
H ½ 2 99.985
Nitrogen 14
N 1 3 99.63
15
N ½ 2 0.37
Vanadium 51
V 7/2 8 99.76
Manganese 55
Mn 5/2 6 100
Iron 57
Fe ½ 2 2.19
Cobalt 59
Co 7/2 8 100
Nickel 61
Ni 3/2 4 1.134
Copper 63
Cu 3/2 4 69.1
65
Cu 3/2 4 30.9
Molybdenum 95
Mo 5/2 6 15.7
97
Mo 5/2 6 9.46
10. Hyperfine splittings multiply with
the number of nuclear spins
O
.
O-
H
H
H
H
Benzoquinone anion radical:
1 proton – splits into 2 lines 1:1
2 protons split into 3 lines 1:2:1
3 protons split into 4 lines 1:3:3:1
4 protons split into 5 lines 1:4:6:4:1
-60 °C
20 °C
At higher temperature:
faster motion - sharper lines
shorter lifetime - smaller signal
13. Prushan Example
SS
N N
OO
B
FF
Cu
[Cu(Thyclops)]+
+
77 K Cryogenic ESR Spectrum of [Cu(Thyclops)]ClO4
in MeOH
Prushan, M. J.; Addison, A. W.; Butcher, R. J.; Thompson, L. K. “Copper(II) Complex Tetradentate Thioether-Oxime Ligands” Inorganica Chimica
Acta, 358, 3449-3456 (2005).
16. If the odd, unpaired electron is associated with a nucleus with nuclear
spin, can get coupling between the two spins and observe 2I+1 (I =
nuclear spin) “peaks” or “valleys”.
Examples:
di-t-butyl nitroxide radical; I(N) = 1;
Hyperfine Splitting
19. superhyperfine splitting
carbon compound; I(C) = 0; 2(0) + 1 = 1 peak…. But:
If the odd, unpaired electron spends time around multiple sets of equivalent
nuclei, additional splitting is observed: 2nI + 1; this is called “superhyperfine
splitting.”
Examples:
Triplet Quartet Pentet
21. It is possible for the unpaired electron to spend differing amounts of time on
different nuclei.
The greater the covalency, the greater is the hyperfine splitting.
Triplet: hyperfine splitting.
Doublet: superhyperfine splitting.
Interpretation: electron is spending most of
its time on CH2 protons, but spending
some time on –OH.
Pentet: hyperfine splitting.
Pentet: superhyperfine splitting.
Interpretation: electron is spending
most of its time on one set of protons,
but spending some time on other set.
22. Septet: hyperfine splitting.
IF= ½, so 2(6)(1/2) + 1 =7
Triplet: superhyperfine splitting.IN= 1, so
2(1)(1) + 1 = 3
So, spending most time on F’s, less on N.
Nonet: hyperfine splitting.
IN= 1, so 2(4)(1) + 1 =9
Pentet: superhyperfine splitting.
IH= 1/2, so 2(4)(1/2) + 1 = 5
So, spending most time on N’s, less on H.
24. High-field high-frequency EPR
X-band Q-band W-band D-band
0.33 1.25 3.5 4.9 Tesla
Bo
Microwave frequency
Superhyperfine interactions become more pronounced!
25. Anisotropic Interactions: The g-tensor
The free electron has a g-value of ge=2.0023
There may be spin-orbit coupling which will effect the ge
lets look at the simple case of Boron, 2p1
.
If all the orbitals have same energy then the spin orbit coupling energy
averages to zero over the x,y, and z coordinate.
However, if the atom is placed in a crystal which removes the degeneracy then
the spin orbit coupling becomes asymmetric, px = py but do not equal to pz
Now the observed g-value will depend
upon orientation of the crystal in the
magnetic field.
26. Axial symmetry
g|| = gz and g⊥ = gx = gy
The g value tells you how strong the electron magnetic tensor is in a given
direction.
Therefore if you orientate the crystal in a different direction the energy to
resonate changes and thus the absorption will shift.
This effect is similar to shielding in the NMR experiment.
The spin-orbit coupling gives a g ⊥ < g || = ge
B
gz
gy
gx B B BB
B B BB
BBBB
BBBB
g ||
g ||
g ⊥
g ⊥
|||| Hgh βν =
||
||
H
h
g
β
ν
=
⊥
⊥ =
H
h
g
β
ν
What happens if the crystal is ground into a
powder?
All orientations are present however there are more
chances that the g ⊥ will be aligned with the field than g ||.
Bo
Bo
z
z
27. ESR spectra of [Cu(MeTtoxBF2)]BF4 in
1:10 BuOH–DMF.
(a) Room temperature (295 K) fluid
spectrum (9.464 GHz). (b) 77 K cryogenic
glass spectrum (9.147 GHz).
Prushan, M. J.; Addison, A. W.*; Butcher, R. J.; "Pentadentate
Thioether Oxime Macrocyclic and Quasi-Macrocyclic Complexes of
Copper(II) and Nickel(II)" Inorganica Chimica Acta, 300-302, 992-1003
(2000).