Ecology is the study of the relationships between organisms and their environment. The biosphere contains all life on Earth, including the interactions between biotic (living) and abiotic (non-living) factors, from individuals to entire ecosystems and biomes. Ecologists use observation, experimentation, and modeling to understand energy flow through trophic levels and the cycling of matter like water, nutrients, carbon, and nitrogen between ecosystems. Population growth rates are influenced by births, deaths, immigration and emigration, and are limited by available resources and carrying capacity.