Cell disruption is an important step in downstream processing and can be achieved through physical, mechanical, or chemical/enzymatic methods. Physical methods include blending, ultrasonication, and freezing and thawing, which use shear forces or cavitation to break open cells. Mechanical methods like French press cells use high pressure to burst cells as they pass through a small orifice. Chemical/enzymatic methods employ enzymes, detergents, osmotic shock, or other chemicals to disrupt cell membranes. Lysozyme is commonly used to break open bacterial cells while zymolyase and lyticase disrupt yeast.
THE PRESENTATION IS ABOUT CELL DISRUPTION THE TOPIC I COVERED IS ABOUT PHYSICAL METHOD OF CELL DISRUPTION OF THERMOLYSIS AND CELL DISRUPTION. THIS COULD BE VALUBLE AND UNDERSTANDABLE.
Organ culture is defined as the description and development in vitro of any organ or in the portion of organs where many tissue constituents like Parenchyma and Stroma, and there structural correlation and function are protected in culture. For what the explanted tissue narrowly look like its parent tissue in vitro.
THE PRESENTATION IS ABOUT CELL DISRUPTION THE TOPIC I COVERED IS ABOUT PHYSICAL METHOD OF CELL DISRUPTION OF THERMOLYSIS AND CELL DISRUPTION. THIS COULD BE VALUBLE AND UNDERSTANDABLE.
Organ culture is defined as the description and development in vitro of any organ or in the portion of organs where many tissue constituents like Parenchyma and Stroma, and there structural correlation and function are protected in culture. For what the explanted tissue narrowly look like its parent tissue in vitro.
Introduction
Importance of Protoplast Isolation
Source of Protoplast
Isolation of Protoplast
Testing the Viability of Isolated Protoplast
Culture of Protoplast
Protoplast Regeneration
Protoplast Fusion
Protoplast Fusion Hybrids:Selection
Cybrids
Practical Applications
Introduction
Importance of Protoplast Isolation
Source of Protoplast
Isolation of Protoplast
Testing the Viability of Isolated Protoplast
Culture of Protoplast
Protoplast Regeneration
Protoplast Fusion
Protoplast Fusion Hybrids:Selection
Cybrids
Practical Applications
The isolation, culture and fusion of protoplasts is a fascinating field in plant research. Protoplast isolation and their cultures provide millions of single cells (comparable to microbial cells) for a variety of studies.
Animal Cell Culture: Growth of animal cells in culture. PHARMACEUTICAL MICROB...Ms. Pooja Bhandare
PHARMACEUTICAL MICROBIOLOGY (BP303T)Unit-VPart-4
Animal Cell Culture: Growth of animal cells in culture.
Introduction: Histroy, The culture media used for animal cell culture are classified as,
Natural, Artificial, Synthesized
Natural Culture Media:
a. Blood Plasma:
b. Blood Serum:
c. Tissue Extracts:
Artificial Media
Some common examples of artificial media are,
Minimal Essential Medium (MEM),
CMRL 1066,
RPMI 1640.
Synthetic media re classified as,
Serum Containing Media.
Serum Free Media.
a. Serum Containing Media:
b. Serum Free Media:
Physicochemical Parameters needed for growth animal cell culture:
General procedure for cell Culture.
Isolation of the tissue:
Disaggregation of the Tissue:
Mechanical disaggregation
b. Enzymatic Disaggregation
. Trypsin based disaggregation or trypsinization:
Warm trypsinization:
Cold trypsinization:
Drawbacks of trypsin disaggregation:
B. Collagenase based disaggregation:
C. Chelating Agents:
3. Seeding of Culture:
General steps in biotechnology: and Various sterilization techniques followed in a tissue culture lab space, such as autoclaving, filtering, flame sterilization, chemical sterilization, UV radiation etc.
who student are interested for slide regarding the biotechnology subject please send mail at shubhammaurya97938@gmail.com
it will help as notes.
this slide will help who love the environment,as well as will help to get the basic knowledge regarding STP.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
3. PHYSICAL-
• Blenders.
• Ultrasonication.
• Freezing thawing.
Blenders-
• This method is ideal for disrupting mammalian or plant tissue by
shear force.
• Tissue are cut in to the small pieces &blended in the presence of
the buffer for about 1 minute to disrupt the tissue.
• Centrifugation is done to remove the cell debris.
• This method is appropriate for the bacterial &yeast cell.
• Cells are trapped b/w chelating beads & are disrupted physically.
4. Grinding mill abrasives-
• Grinding in the mortar in the presence of the san alumina
with small amount of the buffer.
Ultrasonication-
• This method is ideal for the suspension of cultured cell.
• Cell suspension is put on the sonicator probe& high frequency
sound wave for about 20 kHz.
• The sound wave causes the disruption of the cell by shear forces
&cavitation.
• Gas bubble form under the pressure releases the shock wave which
disrupts the plasma membrane.
• This method is suitable for the small volume 50 -100 mtr/cm3.
5. Mechanical method-
French pressure cell-
• It is used in the biological system to extract homogenate
of cell suspension microbial cell &chloroplast.
• It was invented by the Charles Stacy French.
• All cell suspension is passed by the hydrolytic piston
pump under high pressure through the small orifice
pressure is rapidly dropped.
• Compressed cell expend &effectively burst.
• All the cells are under carefully managed under the control
condition & cell release the protein from the periplasmic
space.
6. CHEMICAL OR ENZYMETIC
METHOD-
• Salt.
• Detergent.
• Enzyme.
• Osmotic shock.
Detergent-
• Detergents have been used to disrupt the
cell membrane &extract the lipoprotein.
• It includes the salt such as chelate
nonionic detergent like SDS,CTAB.
7. Enzyme-
• Enzyme lysozyme is isolated from the hen egg
white cleaves the peptidoglycan layer of the
bacteria.
• G- Bacteria can be similarly disrupted by the
lysozyme enzyme.
• The two most commonly enzyme for the yeast
zymolyase &lyticase .
• It consist beta 1.3 gluconase activity.
• Kitinase is used for disrupt filament of the fungi.
8. Polyvenyl pyrolidone-
• PVP is adding to the extraction of the buffer for plant tissue
contain the considerable amount of the phenolic compound.
• This enzyme bind to the co- valent forces including the H
bond, ionic bond hydrophobic bond causing the protein
precipitation.
• This phenolic group is easily oxidized by the phenol oxidase to
form the Quinone which is highly reactive can combined with
reactive group.
• In soluble PVP is added to extraction buffer to absorb the
phenolic compound.
Na –Azide-
• It is added in the buffer for the long period of the period of
the time.
• It acts as the antifungal &antibacterial agent.