New Lawsonia Quantitative PCR Test - Dr. Jessica Seate, Boehringer Ingelheim, from the 2015 World Pork Expo, June 3 - 5, 2015, Des Moines, IA, USA. More presentations at http://www.swinecast.com/2015-world-pork-expo

1. NEW Lawsonia
Quantitative PCR Test
Dr. Jessica Seate

2. • Bacteria- Gram negative curved or
sigmoid rods
• Obligate intracellular pathogen
• Main host species is domestic pig
• Ubiquitous pathogen -World wide
distribution
• Causes subclinical and clinical disease
Lawsonia Intracellularis

3. Lawsonia Intracellularis
“Ileitis”
1.Infection
2.Fecal shedding-7 days
3.Seropositives appear-14 days
4.Clinical signs appear-16 days
5.Cell mediated immunity-14 days
6.Recovery or death-28 days

4. Industry’s 1st L.I. qPCR
• Boehringer Ingelheim Vetmedica, Inc. (BIVI)
Health Management Center HAS DEVELOPED
– Industry’s 1st commercially available
– Quantitative polymerase chain reaction (qPCR)
– Lawsonia Intracellurlaris
– Fecal samples
• Great example of BIVI LEADERSHIP in Enteric
Vaccines and Knowledge!!

5. • Traditional qualitative PCR
– Presence of Lawsonia
• Quantitative PCR
– Bacterial concentration levels of Lawsonia present
– Quantitative PCR uses the same method you’re
used to, with MORE PRECISE standards
Industry’s 1st L.I. qPCR

6. Proven Protection Made Easy
• Perfected the qPCR so able to adjust for PCR
inhibition
– Feed ingredients
– Bacterial constituents in fecal material
RESULT = HIGHLY ACCURATE
= RELIABLE
= REPEATABLE

7. Quantification of L.I shedding-
Why is this important?
Direct correlation between shedding and
severity of lesions

8. Quantification of Lawsonia and
Disease Presentation
1
10
100
1,000
10,000
100,000
1,000,000
10,000,000
100,000,000
clinical ± clincal subclinical convalescence
BacterialConcentrationpergram
ofFeces
Collins, Vet. Microbiol. 2013
NON-VACCINATED ANIMALS

9. Quantification of Lawsonia and
Disease Presentation
1
10
100
1,000
10,000
100,000
1,000,000
10,000,000
100,000,000
clinical ± clincal subclinical convalescence
BacterialConcentrationinFeces

10. 1
10
100
1,000
10,000
100,000
1,000,000
10,000,000
100,000,000
clinical ± clincal subclinical convalescence
BacterialConcentrationinFecesQuantification of Lawsonia and
Disease Presentation

11. 1
10
100
1,000
10,000
100,000
1,000,000
10,000,000
100,000,000
clinical ± clincal subclinical convalescence
BacterialConcentrationinFecesQuantification of Lawsonia and
Disease Presentation
Impact is
underestimated

12. 1
10
100
1,000
10,000
100,000
1,000,000
10,000,000
100,000,000
clinical ± clincal subclinical convalescence
BacterialConcentrationinFecesQuantification of Lawsonia and
Disease Presentation

13. 1
10
100
1,000
10,000
100,000
1,000,000
10,000,000
100,000,000
clinical ± clincal subclinical convalescence
BacterialConcentrationinFecesQuantification of Lawsonia and
Disease Presentation

15. qPCR
Severity of Lawsonia
disease present
Identify
subclinical
pigs
Identify
Lawsonia
disease
throughout the
year
Help in
understanding
economic
losses
Understand
disease
pattern/status
in your herd
Better
immunity
management

16. PROVEN
PROTECTION
MADE EASY
qPCR + Enterisol® Ileitis +
BI Enteric Leadership

17. Acknowledgements
• Health Management Center (HMC), Ames, IA
–Kent Doolittle
–Allison Spitz
–Wayne Chittick
• Dr. Greg Cline
• Mike Steilen

18. Thank you!