MEDICAL MICROBIOLOGY
LABORATORY
Group 6
Nushara Gunasekare, Glen Bowen, Ishini Oasha,
Sureshni Fernando, Ashini Silva and
Dilmi Croos
INTRODUCTION
Purpose of the laboratory
The microbiological processing of patient specimens
and serodiagnostic analyses are fundamental to the
everyday diagnosis, treatment and management of
patients with infectious diseases.
DIAGNOSTIC TESTS
FLOOR PLAN
EQUIPMENT
CentrifugeIncubator Refrigerator
Laminar Flow MicroscopeAutoclave Bunsen burner
Hot air oven
EQUIPMENT MANAGEMENT
Equipment Facility
purchase requirement
Selection and
Acquisition
Installation and
calibration
Maintenance and
repair
Disposition
CHEMICAL MANAGEMENT
Chemical
procurement
Chemical
storage
Chemical
transportation
Chemical
handling
-Freezers
-Cabinets
-Shelves
-Volatile
cabinets
-Pathway is clear
-Naming
chemicals
LABORATORY INFORMATION
MANAGEMENT SYSTEM
 A software that allows the recording of information – test
results, patient information such that it could be processed
and manipulated to assist in the laboratory operations.
Role of LIMS
LABORATORY TESTS
• Urine culture test
• Faecal culture test
• Sputum culture test
• Blood culture test
OBTAIN YOUR TEST RESULTS ONLINE
Reference no.
Passcode
ARTICLES
OFFERS
• Healthcare packages
WEBSITE
WASTE MANAGEMENT
 Large amounts of waste will not be accumulated within the
laboratory and thus be stored in a suitable area prior to
collection.
 The disposal will be in accordance with
national environmental protection regulations.
 All disposal will be recorded on a Waste Tracking Log.
REGULATIONS
 Control of Substances Hazardous to Health Regulations
(COSHH - 2002)
 ISO 15189:2012
 Accreditation from Sri Lanka Accreditation Board (SLAB)
 Good Laboratory Practice (GLP)
• Identify hazard
• Analyze and evaluate
• Prevent and minimize
Risk assessment
• Fire extinguisher
• Eye wash
• Emergency showers
Safety aspects
• Lab coat
• Gloves
• Safety goggles
Personal
protective
equipment
RISK AND SAFETY
 Vaccines – MMR, Hib, IPV
 5% discount for senior citizens
 Healthcare packages
 Running tests for medical centres
 Reduce cost for equipment
MARKETING STRATEGIES
HUMAN RESOURCES
 Laboratory Consultant
 Laboratory Manager
 Laboratory Technician
 Laboratory Assistant
 Nurse
 Minor Staff
Staff Member Job Description Qualifications Registration
Consultant • Confirms and approves test
results
• MBBS
• 5 Year Experience
SLMC (Sri Lankan
Medical Council)
certification
Nurse • Sample collection
• Customer care
• B. Sc Nursing / Nursing
course
• 1 Year experience
Laboratory
Manager
• Overview of Laboratory
Processes
• Set targets and goals
• HR Management
• Ph. D / M. Sc
• 5 Year Work
Experience
SLMC (Sri Lankan
Medical Council)
certification
Assistant
Manager
• Implement Strategies to
reach goals
• Work in coordination with
employees
• Equipment Management
• M. Sc
• B. Sc
• 2 Year Work
Experience
Laboratory
Technician
• Processing Samples
• Interpret Test Results
• B. Sc
• 1 Year Work Experience
• Fresh graduates /
Intern
Laboratory
Assistant
• Maintain Equipment
• Data entry
• Media preparation
• A/Ls
• Undergraduate
RECRUITMENT AND PERFORMANCE
TESTING
 Selection – Interview, Practical Sessions and Questionnaires
 Probation period – 6 months
 Performance Testing - Performance reviews
- Internal Audits
- External Audits
TRAINING AND EMPLOYEE BENEFITS
 Training programs
- conducted by Consultant Doctors (Monthly)
 Employee Benefits
- Overtime Payment
- Health Assurance
- Staff transport
 Interns – Certificate with service letter and allowance
(food and transport)
SUMMARY
Initiation Setting up Management
Human
resources
REFERENCES
• Acikgoz, Y. (2019) ‘Employee recruitment and job search: Towards a multi-level integration’ 29(1), pp.1-13
Human Resource Management Review [Online] Available at:
https://www.sciencedirect.com/science/article/pii/S1053482218301190 (Accessed: 10th July 2019).
• Canadian Centre for Occupational Health and Safety (2017) ‘Risk assessment’ Available at:
https://www.ccohs.ca/oshanswers/hsprograms/risk_assessment.html (Accessed: 8 July 2019).
• Cannon hygiene (no date) Waste not, Want not – Where does your clinical waste actually go? Available at:
https://www.cannonhygiene.com/support/knowledge-centre/waste-not-want-not%E2%80%93-where-does-
your-clinical-waste-actually-go (Accessed: 10 July 2019).
• Chinniah, T. R. (2009) ‘Blood Culture Techniques: Increasing Yield and Reducing Contamination’ 1, 1, 15-24, Sri
Lanka Journal of Critical Care [Online]. Available at:
https://sljcc.sljol.info/article/10.4038/sljcc.v1i1.938/galley/890/download/ (Accessed: 10 July 2019).
• Chute, W. B. and Wunnava, P. V. (2018) ‘Is there a link between Employer Provided Health Insurance and Job-
Mobility? Evidence from NLSY79’ Open Journal of Human Resource Management, 1(1), pp. 38-52 [Online].
Available at: https://www.sryahwapublications.com/open-journal-of-human-resource-management/volume-1-
issue-1/5 (Accessed: 5th July 2019)
• Climet Instruments Company (2018) LIMS. Available at: http://www.climet.com/software/LIMS.html
(Accessed: 14 July 2019).
• Fung, J., Lucia, B., Clark, E., Berman, M., Goldstein, J. and D'amato, R. F. (1982) ‘Primary Culture Media for
Routine Urine Processing’ Journal of Clinical Microbiology 16, 4, 632-636 [Online]. Available at:
https://jcm.asm.org/content/jcm/16/4/632.full.pdf (Accessed: 5 July 2019).
• Gibbon, G. A. (1996) ‘A brief history of LIMS’, Laboratory Automation and information management, 32(1), pp.
1-5 [Online]. Available at: https://www.sciencedirect.com/science/article/abs/pii/1381141X9500024K (Accessed:
30 June 2019).
• Illumina (2018) Introduction to Lab Information Management Systems. Available at:
https://www.illumina.com/informatics/sample-experiment-management/lims.html (Accessed: 14 July 2019).
REFERENCES
• Kauffman, C. A., Fisher, J., Sobel, J. and Newman, C. A. (2011) ‘Candida Urinary Tract Infections—
Diagnosis’ Clinical Infectious Diseases 52, 6, 452–456, Oxford Academic [Online]. Available at:
https://academic.oup.com/cid/article/52/suppl_6/S452/285004#97948438 (Accessed: 5 July 2019).
• Kumari, i. G., & Pradhan, r. K. (2014) ‘Human resource flexibility and Organizational Effectiveness: role of
Organizational Citizenship Behaviour and Employee intent to Stay’ International Journal of Business and
Management Invention, 11(3), pp. 43-51. [Online] Available at:
https://www.researchgate.net/publication/308606912_Human_Resource_Flexibility_and_Organizational_Eff
ectiveness_Role_of_Organizational_Citizenship_Behaviour_and_Employee_Intent_to_Stay (Accessed: 9th
July 2019).
• Mathis, R. and Jackson, J. (2008) Human resource management. Mason OH: Thomson/South-western
(Accessed: 8 July 2019).
• Medical Laboratory Observer (2009) Waste management for the clinical lab. Available at: https://www.mlo-
online.com/waste-management-for-the-clinical-lab.php (Accessed: 5 July 2019).
• NHS Foundation Trust (no date) Blood culture Available at: https://www.ouh.nhs.uk/microbiology/diagnostic-
tests/atoz/blood-culture.aspx#method (Accessed: 10 July 2019).
• Optochin Sensitivity Test: Principle, Procedure, Results and Quality Control (2013) Available at:
https://microbeonline.com/optochin-test-principle-procedure-expected-results-and-quality-control/
(Accessed: 7 July 2019).
• Sputum Culture (2014) Available at: https://emedicine.medscape.com/article/2119232-overview (Accessed: 5
July 2019).
• Student Sri Lanka Education (2019) How to become a Nurse [Online] Available at:
https://studentlanka.com/2010/11/11/career-in-nursing-enter-a-nursing-school-and-become-a-nurse/ [Accessed:
14 Jul. 2019].
Designing a Microbiology laboratory

Designing a Microbiology laboratory

  • 1.
    MEDICAL MICROBIOLOGY LABORATORY Group 6 NusharaGunasekare, Glen Bowen, Ishini Oasha, Sureshni Fernando, Ashini Silva and Dilmi Croos
  • 2.
    INTRODUCTION Purpose of thelaboratory The microbiological processing of patient specimens and serodiagnostic analyses are fundamental to the everyday diagnosis, treatment and management of patients with infectious diseases.
  • 3.
  • 4.
  • 5.
    EQUIPMENT CentrifugeIncubator Refrigerator Laminar FlowMicroscopeAutoclave Bunsen burner Hot air oven
  • 6.
    EQUIPMENT MANAGEMENT Equipment Facility purchaserequirement Selection and Acquisition Installation and calibration
  • 7.
  • 8.
  • 9.
    LABORATORY INFORMATION MANAGEMENT SYSTEM A software that allows the recording of information – test results, patient information such that it could be processed and manipulated to assist in the laboratory operations.
  • 10.
  • 11.
    LABORATORY TESTS • Urineculture test • Faecal culture test • Sputum culture test • Blood culture test OBTAIN YOUR TEST RESULTS ONLINE Reference no. Passcode ARTICLES OFFERS • Healthcare packages WEBSITE
  • 12.
    WASTE MANAGEMENT  Largeamounts of waste will not be accumulated within the laboratory and thus be stored in a suitable area prior to collection.  The disposal will be in accordance with national environmental protection regulations.  All disposal will be recorded on a Waste Tracking Log.
  • 14.
    REGULATIONS  Control ofSubstances Hazardous to Health Regulations (COSHH - 2002)  ISO 15189:2012  Accreditation from Sri Lanka Accreditation Board (SLAB)  Good Laboratory Practice (GLP)
  • 15.
    • Identify hazard •Analyze and evaluate • Prevent and minimize Risk assessment • Fire extinguisher • Eye wash • Emergency showers Safety aspects • Lab coat • Gloves • Safety goggles Personal protective equipment RISK AND SAFETY
  • 16.
     Vaccines –MMR, Hib, IPV  5% discount for senior citizens  Healthcare packages  Running tests for medical centres  Reduce cost for equipment MARKETING STRATEGIES
  • 17.
    HUMAN RESOURCES  LaboratoryConsultant  Laboratory Manager  Laboratory Technician  Laboratory Assistant  Nurse  Minor Staff
  • 18.
    Staff Member JobDescription Qualifications Registration Consultant • Confirms and approves test results • MBBS • 5 Year Experience SLMC (Sri Lankan Medical Council) certification Nurse • Sample collection • Customer care • B. Sc Nursing / Nursing course • 1 Year experience Laboratory Manager • Overview of Laboratory Processes • Set targets and goals • HR Management • Ph. D / M. Sc • 5 Year Work Experience SLMC (Sri Lankan Medical Council) certification Assistant Manager • Implement Strategies to reach goals • Work in coordination with employees • Equipment Management • M. Sc • B. Sc • 2 Year Work Experience Laboratory Technician • Processing Samples • Interpret Test Results • B. Sc • 1 Year Work Experience • Fresh graduates / Intern Laboratory Assistant • Maintain Equipment • Data entry • Media preparation • A/Ls • Undergraduate
  • 19.
    RECRUITMENT AND PERFORMANCE TESTING Selection – Interview, Practical Sessions and Questionnaires  Probation period – 6 months  Performance Testing - Performance reviews - Internal Audits - External Audits
  • 20.
    TRAINING AND EMPLOYEEBENEFITS  Training programs - conducted by Consultant Doctors (Monthly)  Employee Benefits - Overtime Payment - Health Assurance - Staff transport  Interns – Certificate with service letter and allowance (food and transport)
  • 21.
    SUMMARY Initiation Setting upManagement Human resources
  • 22.
    REFERENCES • Acikgoz, Y.(2019) ‘Employee recruitment and job search: Towards a multi-level integration’ 29(1), pp.1-13 Human Resource Management Review [Online] Available at: https://www.sciencedirect.com/science/article/pii/S1053482218301190 (Accessed: 10th July 2019). • Canadian Centre for Occupational Health and Safety (2017) ‘Risk assessment’ Available at: https://www.ccohs.ca/oshanswers/hsprograms/risk_assessment.html (Accessed: 8 July 2019). • Cannon hygiene (no date) Waste not, Want not – Where does your clinical waste actually go? Available at: https://www.cannonhygiene.com/support/knowledge-centre/waste-not-want-not%E2%80%93-where-does- your-clinical-waste-actually-go (Accessed: 10 July 2019). • Chinniah, T. R. (2009) ‘Blood Culture Techniques: Increasing Yield and Reducing Contamination’ 1, 1, 15-24, Sri Lanka Journal of Critical Care [Online]. Available at: https://sljcc.sljol.info/article/10.4038/sljcc.v1i1.938/galley/890/download/ (Accessed: 10 July 2019). • Chute, W. B. and Wunnava, P. V. (2018) ‘Is there a link between Employer Provided Health Insurance and Job- Mobility? Evidence from NLSY79’ Open Journal of Human Resource Management, 1(1), pp. 38-52 [Online]. Available at: https://www.sryahwapublications.com/open-journal-of-human-resource-management/volume-1- issue-1/5 (Accessed: 5th July 2019) • Climet Instruments Company (2018) LIMS. Available at: http://www.climet.com/software/LIMS.html (Accessed: 14 July 2019). • Fung, J., Lucia, B., Clark, E., Berman, M., Goldstein, J. and D'amato, R. F. (1982) ‘Primary Culture Media for Routine Urine Processing’ Journal of Clinical Microbiology 16, 4, 632-636 [Online]. Available at: https://jcm.asm.org/content/jcm/16/4/632.full.pdf (Accessed: 5 July 2019). • Gibbon, G. A. (1996) ‘A brief history of LIMS’, Laboratory Automation and information management, 32(1), pp. 1-5 [Online]. Available at: https://www.sciencedirect.com/science/article/abs/pii/1381141X9500024K (Accessed: 30 June 2019). • Illumina (2018) Introduction to Lab Information Management Systems. Available at: https://www.illumina.com/informatics/sample-experiment-management/lims.html (Accessed: 14 July 2019).
  • 23.
    REFERENCES • Kauffman, C.A., Fisher, J., Sobel, J. and Newman, C. A. (2011) ‘Candida Urinary Tract Infections— Diagnosis’ Clinical Infectious Diseases 52, 6, 452–456, Oxford Academic [Online]. Available at: https://academic.oup.com/cid/article/52/suppl_6/S452/285004#97948438 (Accessed: 5 July 2019). • Kumari, i. G., & Pradhan, r. K. (2014) ‘Human resource flexibility and Organizational Effectiveness: role of Organizational Citizenship Behaviour and Employee intent to Stay’ International Journal of Business and Management Invention, 11(3), pp. 43-51. [Online] Available at: https://www.researchgate.net/publication/308606912_Human_Resource_Flexibility_and_Organizational_Eff ectiveness_Role_of_Organizational_Citizenship_Behaviour_and_Employee_Intent_to_Stay (Accessed: 9th July 2019). • Mathis, R. and Jackson, J. (2008) Human resource management. Mason OH: Thomson/South-western (Accessed: 8 July 2019). • Medical Laboratory Observer (2009) Waste management for the clinical lab. Available at: https://www.mlo- online.com/waste-management-for-the-clinical-lab.php (Accessed: 5 July 2019). • NHS Foundation Trust (no date) Blood culture Available at: https://www.ouh.nhs.uk/microbiology/diagnostic- tests/atoz/blood-culture.aspx#method (Accessed: 10 July 2019). • Optochin Sensitivity Test: Principle, Procedure, Results and Quality Control (2013) Available at: https://microbeonline.com/optochin-test-principle-procedure-expected-results-and-quality-control/ (Accessed: 7 July 2019). • Sputum Culture (2014) Available at: https://emedicine.medscape.com/article/2119232-overview (Accessed: 5 July 2019). • Student Sri Lanka Education (2019) How to become a Nurse [Online] Available at: https://studentlanka.com/2010/11/11/career-in-nursing-enter-a-nursing-school-and-become-a-nurse/ [Accessed: 14 Jul. 2019].

Editor's Notes

  • #3 Good Morning Today we are here to present to you our proposal for a microbiology lab. We have chosen a microbiology lab specifically, since the Organisational structure We are proposing this idea as a joint venture where we will pool in our resources to benefit both parties. We will be managaing the human resources and providing the experience required to maintain quality and efficiency within the lab. Financial support and space allocations.
  • #4 The diagnostic tests run at our lab will be divided according to the samples we receive. For example, once we receive a sample of urine at our lab, the first step would be to culture it. For this we would use a growth medium such as PDA or MacConkey Agar. This process will take up to 24 hours depending on the sample. After culturing, the microbial growth will be observed. This will also include further testing such as motility indole urease tests, oxidase test and simmons citrate test. API wil be done as a cofirmatory test.
  • #13 All generated waste is clinical and will be divided into plastic, glass, sharps and others at the laboratory. Sisili projects consortium will collect waste twice a week and incinerate them in an environmental friendly manner.