The document summarizes an experiment validating an anti-β-actin antibody for immunocytochemistry. Rat cortical cells were fixed and permeabilized before probing with the STJ96930 anti-β-actin primary antibody at a 1:100 dilution overnight at 4°C. A fluorescent secondary antibody was then applied before visualization, which showed staining below the plasma membrane as expected for β-actin. The antibody was deemed to specifically label β-actin based on the staining pattern.