This document is a curriculum vitae for Ferretti Massimo that includes his personal details, education history, work experience, skills, publications, and other qualifications. It summarizes that he received a PhD in Molecular Medicine in 2008, has worked in development biology at Diasorin since 2010, has experience in molecular biology techniques, cell culture, and managing biological laboratories, and has authored 11 scientific publications.

1. Curriculum Vitae et Studiorum
January 2017
Personal data
Name: Ferretti Massimo
Birthdate: Alessandria (AL), June 15, 1977
e-mail: massimo.ferretti@diasorin.it;
Education and training
2008: PhD in Molecular Medicine, XIX cycle, at the Department of Medical Sciences in Novara,
University of Eastern Piedmont, Immunology Lab (Prof. U. Dianzani). Dissertation: “The 423Q
polymorphism of the XIAP gene influences monocyte function and is associated with Periodic Fever”.
2001: Degree in Biological Sciences at the Faculty of Sciences M.F.N. University of Eastern Piedmont,
Department of Alessandria, graduated 108/110. Dissertation: “Characterization of a single-chain
intrabody directed against the human receptor tyrosine kinase Ron.”
1996: High school graduation at Liceo Scientifico “G. Galilei”, Alessandria.
Working Experiences
Since September 2010: Responsible of Development Biology and Banks in Diasorin s.p.a.,
Saluggia, Italy.
Since May 2008: Employed in Development Biology and Banks in Diasorin s.p.a., Saluggia,
Italy.
November 2003/October 2007: PhD student in the Immunology laboratory, directed by Prof.
Umberto Dianzani, University of Eastern Piedmont, Department of Medical Sciences, XIX cycle, Italy.
March 2002/November 2003: Contract of collaboration with the University of Eastern Piedmont, at
Faculty of Biotechnology as scientific technician, D1 category.
November 2001/February 2002: Contract of collaboration with the Innosense, Ivrea. Efficiency
screening of reagents Lipofectamin-like for cellular transfection.
September 1999/June 2001: Attending student in the Applied Biology laboratory, directed by Prof.
Claudio Santoro, at the department of Medical Sciences, University of Eastern Piedmont,
Novara.

2. Acquired experiences
· Optical, confocal and fluorescence microscopy;
· Molecular biology techniques and colture with bacteria (coltures, transformation, protein
extraction and purification, fermentation, optimization and scale- up planning and processing);
· Molecular biology techniques with Phagemid libraries and phage display antibody system;
optimization and production of large scale of T4 and Q-beta phages;
· Cellular biology techniques (coltures optimization, transfection, protein extraction and
purification, radioactive protein labeling, nuclear extraction, hollow fiber processes,
colorimetric/fluorescence in vitro labelling);
· Molecular biology techniques in DNA, RNA and proteins manipulation (PCR, RT-PCR, sequencing,
DNA and RNA extraction, plasmid construction, SDS-PAGE, Western Blot, ELISA, protein purification,
immunoprecipitation, PEGylation, enzyme assays).
· Cytofluorimetry (routine analysis and sorting techniques);
· Blood samples and primary coltures manipulations (ficoll separation, coltures, differentiation,
proliferation and functional assays);
· Manipulation of HIV and chronically infected cells line (infections, acute and chronically infection
assays);
· Management of biological laboratory and personnel, process development and optimization;
· Writing of Standard Operating Procedures in compliance with GMP quality system and FDA
guidances;
Seminaries
· Seminary of “Diritto Amministrativo di Base” organized by University of Eastern Piedmont;
· Seminary of “Rischio biologico e biotecnologie in Laboratorio” organized by CO.IN.FO. University
of Pavia;
· Seminary of “Protezione dei lavoratori dal rischio biologico”, organized by Istituto Ambiente
Europa.
Languages
English: good.
Informatics knowledge
Operative systems (Windows): Basic
Utility software (Word, Excel…): Basic

3. Presentation at conferences
· A CD38-derived peptide as a potential antiretroviral agent exploiting cellular mechanisms of
antiviral defense. Andrea Savarino, Thea Bensi, Massimo Ferretti, Umberto Dianzani. National
congress SIICA, Trieste 2007, Italy;
· High levels of XIAP associated to a polymorphism in its gene are a risk factor for development of
periodic fever. Massimo Ferretti, Marco Gattorno, Annalisa Chiocchetti, Silvia Federici, Isabella
Ceccherini, Maurizia Baldi, Alberto Martini, Umberto Dianzani. 5ft international congress FMFSAID,
Rome 2008, Italy.
Military service
Performed with substitutive civil service at the Associazione Italiana Sclerosi Multipla (AISM),
Alessandria.
Collaborations and Professional Training Courses
· December 2009- January 2010: Collaboration with Kungliga Tekniska Hogskolan (KTH) Royal
Institute of Technology – Stockholm – Sweden, for improving bacterial fedbatch fermentation
strategies;
· 16-22 June 2011: Collaboration with Kungliga Tekniska Hogskolan (KTH) Royal Institute of
Technology – Stockholm – Sweden, for improving cultivation and fermentation of P. Pastoris;
· 24-27 May 2011: Advanced Bioreactor Cultivaton Course (GE Healthcare) – Munich – Germany;
· 23-28 June 2013: Collaboration with Bioventix – Dartford – UK, collaboration for improving sheep
hybridomas cultivation methods;
· 14-16 October 2013: Rapid Fermentation Process Design: From development to Manufacture,
Course (MBI – Advanced Centre of Biochemical Engineering, University College of London) – London
– UK.
Scientific publications
1. Secco P, Ferretti M, Gioia D, Bozzo C, Marks JD, Santoro C. Characterization of a single chain
intrabody directed against the human receptor tyrosine kinase Ron. J Immunol Methods. 2004 Feb
1;285(1): 99-109
2. Comi C, Gaviani P, Leone M, Ferretti M, Castelli L, Mesturini R, Ubezio G, Chiocchetti A, Osio M,
Muscia F, Bogliun G, Corso G, Gavazzi A, Mariani C, Monaco F, Dianzani U. Fas-mediated T-cell
apoptosis is impaired in patients with chronic inflammatory demyelinating polyneuropathy. J
Peripher Nerv Syst. 2006 Mar;11(1):53-60
3. Clementi R, Chiocchetti A, Cappellano G, Cerutti E, Ferretti M, Orilieri E, Dianzani I, Ferrarini M,
Bregni M, Danesino C, Bozzi V, Putti MC, Cerutti F, Cometa A, Locatelli F, Maccario R, Ramenghi U,
Dianzani U. Variation of the perforin gene in patients with autoimmune/lymphoproliferation and
detective fas function. Blood. 2006 Nov 1;108(9):3079-84
4. Mesturini R, Nicola S, Chiocchetti A, Bernardone IS, Castelli L, Bensi T, Ferretti M, Comi C, Dong
C, Rojo JM, Yagi J, Dianzani U. ICOS cooperates with CD28, IL2 and INFgamma and modulates
activation of human naive CD4+ T cells. Eur J immunol. 2006 Oct.;36(10):2601-12.
5. DeFranco S, Chiocchetti A, Ferretti M, Castelli L, Cadario F, Cerutti F, Rabbonì I, Indelicato M,
Mazzarino C, Chessa M, Bona G, Dianzani U. Detective function of the Fas apoptotic pathway in Type

4. I diabetes mellitus correlates with age at onset. Int J Immunopathol Pharmacol. 2007; 20 (3), 567-
576.
6. Zavattaro E, Azzimonti B, Mondini M, DeAndrea M, Borgogna C, Dell’Oste V, Ferretti M, Nicola S,
Cappellano G, Carando A, Leigheb G, Landolfo S, Dianzani U, Gariglio M. Identification of detective
Fas function and variation of Perforin gene in an Epidermodysplasia Verruciformis patient lacking
EVER1 and EVER2 mutations. Journal of Investigative Dermatology. 25 Ottobre 2007
7. Cerutti E, Campagnoli MF, Ferretti M, Garelli E, Crescenzino N, Rosolen A, Chiocchetti A. Lenardo
M, Ramenghi U, Dinazani U. Co-inherited mutations of Fas and caspase-10 in development of the
autoimmune lymphoproliferative syndrome. BMC Immunolgy. 2007 Nov. 13; 8(1):28
8. Orilieri E, Cappellano G, Clementi R, Cometa A, Ferretti M, Cerutti E, Cadario F, Martinetti M,
Larizza D, Calcaterra V, D’Annunzio G, Lorini R, Cerutti F, Bruno G, Chiocchetti A, Dianzani U.
Variation of the perforin gene in patients with Type 1 Diabetes. Diabetes 2008, Apr; 57(4):1078-83
9. Bensi T, Mele F, Ferretti M, Novelli S, El Daker S, Chiocchetti A, Maria Rojo J, cauda R, Dianzani U,
Savarino A. Evaluation of the antiretroviral effects of a PEG-conjugate peptide derived from human
CD38. Exper Opin Ther Targets. 2009 Feb; 13(2): 141-52
10. Comi C, Osio M, Ferretti M, Mesturini R, Cappellano G, Chiocchetti A, Carecchio M, Nascimbene
C, Varrasi C, Cantello R, Mariani C, Monaco F, Dianzani U. Defective Fasmediated T-cell apoptosis
predicts acute onset CIDP. J Peripher Nerv Syst. 2009 Jun; 14(2): 101-6.
11. Ferretti M, Gattorno M, Chiocchetti A, Mesturini R, Orilieri E, Bensi T, Sormani MP, Cappellano
G, Cerutti E, Nicola S, Biava A, Bardelli C, Federici S, Ceccherini I, Baldi M, Santoro C, Dianzani I,
Martini A, Dianzani U. The 423Q polymorphism of the XIAP gene influences monocyte function and
is associated with Periodic Fever. Arthritis and Rheumatism. 2009 Oct.29;60(11): 3476-3484.
Ferretti Massimo