1. Curriculum Vitae Gabriella Di Stefano PhD
Via Crocelle 35, 66040 Castelguidone (Italy)
(+39) 0873364468 (+39) 3887323728
distegab200382@gmail.com
Skype gabriella200382
Sex Female | Date of birth 20/03/1982 | Nationality Italian
WORK EXPERIENCE
EDUCATION
9/2015–11/2015 Instructor of Italian language and culture in Refugees Camp
Consorzio Matriz, Schiavi d`Abruzzo (CH) Italy
6/2015–9/2015 Waitress
Pizzeria Solidale "San Vito" API Abruzzo Castelguidone (CH) Italy
3/2013–3/2015 Research scholar
Departments of Medicine and Pathology Case Western Reserve University Division of
Gastroenterology and Liver Disease Case Western Reserve University Cleveland OH USA
2009–2011 Voluntary instructor for pupils and high school students in Biological disciplines
4/2011–10/2011 Qualifying period in the Laboratory of Clinical Pathology
Department of Life, Health and Environmental Sciences, University of Study of L`Aquila
L`Aquila (AQ) Italy
7/2010–3/2011 Qualifying period in the Laboratory of Clinical Analysis Bioanalisi Vasto (CH) Italy
1/2009–11/2010 Qualifying period in the Immunohaematology and Transfusion Service
San Salvatore Hospital L`Aquila (AQ) Italy
4/2010–6/2010 Instructor of 1st and 2nd year Beauty Care students Monfor Institute Vasto (CH) Italy
Courses: biology, anatomy, chemistry, cosmetology
1/2009–12/2009 Servizio Civile Nazionale Consorzio Celestiniano L`Aquila (AQ) Italy
6/2007–10/2007 Qualifying period in Laboratory of Clinical Analysis Histonium Vasto, Italy
5/2006–8/2006 Qualifying period in the Laboratory of Pathological Anatomy San Salvatore Hospital
L`Aquila, Italy
11/2015 -until now Spanish course
University of Tre Eta` Centro Culturale Aldo Moro, San Salvo (CH) Italy

2. Curriculum Vitae Gabriella Di Stefano Page 2 / 4
PERSONAL SKILLS
11/2011–10/2014 PhD in Traslational Medicine
Department of Life, Health and Environmental Sciences University of Study of L`Aquila
Piazzale Salvatore Tommasi 1 - Coppito, 67100 L`Aquila (AQ) Italy
Thesis: Novel experimental models to investigate immune mechanisms involved in the
pathogenesis of Crohn`s Disease.
09/2013-03/2015
2009-2011
November 2008
2005–2008
English course
Cuyahoga Community College 2900 Community College Ave, Cleveland
44115 OH USA
Post-graduate student in School of Clinical Pathology
University of Study of L`Aquila Piazzale Salvatore Tommasi 1 - Coppito,
67100 L`Aquila (AQ) Italy
Esame di Stato per l`abilitazione all`Albo dei Biologi (Sezione A)
Universita` degli Studi dell`Aquila Piazzale Salvatore Tommasi 1 - Coppito,
67100 L`Aquila (AQ) Italy
Master`s degree in Biology (Biologia Sanitaria)
University of Study of L`Aquila Piazzale Salvatore Tommasi 1 - Coppito, 67100 L`Aquila
(AQ) Italy – Immunohaematology and Transfusion Service San Salvatore Hospital
Thesis: Validation of blood unity and management of emo-components.
2001–2004 Bachelor`s degree in Biology
University of Study of Perugia, Piazza Universita` 1, 06100 Perugia (PG) Italy
Thesis: Breath test for hydrogen peroxide dosage.
1997–2001 Maturita` classica
Liceo Ginnasio Lucio Valerio Pudente, Via dei Conti Ricci 1, 66054 Vasto (CH) Italy
Research Area
▪ Skin wound healing and tissue repair
▪ Platelet Rich Plasma (PRP)
▪ Inflammatory Bowel Diseases (IBD)
Mother tongue(s) Italian
Other language(s) UNDERSTANDING SPEAKING WRITING
Listening Reading Spoken interaction Spoken production
English C1 C1 C1 C1 C1
French A2 A2 A1 A1 A1
Levels: A1 and A2: Basic user - B1 and B2: Independent user - C1 and C2: Proficient user
Common European Framework of Reference for Languages

3. Technical skills Molecular genetic: DNA and RNA extraction from human, mouse and bacteria samples;
PCR, cDNA, and RT-PCR.
Biochemistry: Spectrophotometric enzyme assays; Gel electrophoresis; Gelatin and Cysteine
Protease zymography; Proliferation assay; Western blot; Immunoprecipitation; ELISA; EIA;
Myeloperoxidase Assay Activity, and sample preparation for Flow Cytometry .
Immunostaining: Immunohistochemistry.
Optical Microscopy: sample preparation; tissue sectioning; staining (Haematoxylin and Eosin
and Trichrome staining); and detection of human, and mouse samples.
Electron Microscopy: sample preparation; tissue sectioning; staining; and detection.
Microbiology: bacteria culture; in vitro/in vivo bacteria treatment and in vivo/in vitro
probiotics treatment, and Gram, and bacteria staining (Gram, and Ziehl–Neelsen).
Tissue and cell Culture: isolation and culture of human HUVEC, fibroblast, tenocytes,
ketatinocytes, and PBMCs; isolation and culture of mouse MLN cells, splenocytes and BM
macrophages; isolation and culture of primary tumor cells.
Blood haemoderivates: blood processing in emocomponent, Platelet Rich Plasma and Platelet
Rich Plasma Surnatant preparation.
Wound closure and Chemotaxis : Scratch wound closure assay; motility and invasion test in
Boyden chamber.
Membrane Vesicles (MV): isolation of MV from conditioned medium from tumor cells.
Animal work: mouse crossing; handling and genotyping; tail vein and oral gavage model.
Procedures in clinical laboratory: pre-analytical; analytical and post-analytical; internal and
external quality control; blood bank (cross-match; blood grouping; preparation of
haemoderivates); microscope reading haematology; coprology and urinary parasite; urinary
sediment and secreted, exudate; sowing and reading methods for executing bacteriological
tests on urine, feces and various biological materials, including GRAM bacteriological
identification; sierology; clinical chemistry; coagulation; haematology; electrophoresis
(pathological haemoglobin); urine and virology tests.
Digital competence SELF-ASSESSMENT
Information
processing
Communication
Content
creation
Safety
Problem
solving
Proficient user Proficient user Proficient user Independent user
Digital competences - Self-assessment grid
Windows and Mac: Microsoft Office (Excel, Word and PowerPoint) and Adobe Creative
(Photoshop and Illustrator)
Driving licence B

4. Curriculum Vitae Gabriella Di Stefano Page 4 / 4
ADDITIONAL
INFORMATION
Publications (3 pubblications on indexed journals plus one submitted, 2 conference presentations)
1.Occurrence of Spontaneous Periodontal Disease in the SAMP1/YitFc Murine Model of
Crohn's Disease.
Pietropaoli D, Del Pinto R, Corridoni D, Rodriguez-Palacios A, Di Stefano G, Monaco A,
Weinberg A, Cominelli F. Journal of Periodontology December 2014, Vol. 85, No. 12, Pages
1799-1805.
2.Platelet concentration in platelet-rich plasma affects tenocyte behavior in vitro.
Giusti, D'Ascenzo S, Mancò A, Di Stefano G, Di Francesco M, Rughetti A, Dal Mas A,
Properzi G, Calvisi V, Dolo V. BioMed Research International Volume 2014 (2014).
3.The effects of platelet gel–released supernatant on human fibroblasts.
Ilaria Giusti PhD, Anna Rughetti MD, Sandra D'Ascenzo PhD, Gabriella Di Stefano Dr,
Maria Rita Nanni MD, Danilo Millimaggi PhD, Luigi Dell'Orso MD and Vincenza Dolo PhD
Wound Repair and Regeneration Volume 21, Issue 2, pages 300–308, March-April 2013.
Submitted paper
1. NOD2 promotes Th2-mediated intestinal inflammation independently from
acute dysbiosis.
*Daniele Corridoni, *Alexander Rodriguez-Palacios, Gabriella Di Stefano, Luca Di
Martino, Dionysios A. Antonopoulos, Eugene B. Chang, Theresa T. Pizarro, and Fabio
Cominelli. * DC and AR-P share first authorship
Conferences 1. NOD2 plays a pro-inflammatory role in the chronic phase of Crohn's-like ileitis by a
mechanism involving Th2 cytokines.
Daniele Corridoni, Davide Pietropaoli, Gabriella Di Stefano, Alexander Rodriguez-Palacios,
Mitchell Guanzon, Paola Menghini, Wei Xing, Theresa T. Pizarro, and Fabio Cominelli,
Poster presentation and talk Digestive Disease Week (DDW) May 3 - 6, 2014; Chicago,
Illinois.
2. Efficacia del gel piastrinico nel trattamento delle ulcere cutanee croniche ad eziologia
post-traumatica.
A. Rughetti, M. R. Nanni, A. Barrucci, M. Zecca, G. Di Stefano, R. Costantini, A. Lupo, L.
Dell'Orso. Blood Transfusion 2009, 7 Suppl. 2, May. Conferenza Nazionale dei Servizi
Trasfusionali Terrasini, 28-30 Maggio 2009 Palermo, Italy.
References - Daniele Corridoni Postdoctoral Scientist, Institute of Molecular Medicine University of
Oxford John Radcliffe Hospital Headington Oxford daniele.corridoni@ndm.ac.ox.uk
- Fabio Cominelli Professor, Departments of Medicine and Pathology Division of
Gastroenterology and Liver Disease, Case Western Reserve University 11100 Euclid Avenue
44016 Cleveland Ohio USA fabio.cominelli@uhhospitals.org +1 216-844-7344
- Maria Grazia Cifone Professor, Department of Life, Health and Environmental Sciences
University of Study of L`Aquila Piazzale Salvatore Tommasi 1, Coppito L`Aquila, Italy
cifone@univaq.it +39 0862- 433301
- Vincenza Dolo Professor, Department of Life, Health and Environmental Sciences
University of Study of L`Aquila Piazzale Salvatore Tommasi 1, Coppito L`Aquila, Italy
dolo@univaq.it +39 0862- 433666