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OFFICIAL TRANSLATION
EVMORFIA G. TZAGKARAKI
Molecular Biologist, M.Sc., PhD
Mob. +30 697 4862 199
Email: etzagkaraki@outlook.com
PERSONAL INFORMATION:
Date of Birth: April 5, 1980
Nationality: Greek
Marital status: Unmarried
Permanent residence: Rethymnon, Crete, Greece
Molecular biologist - Laboratory geneticist with 6 years’ experience in the application
of molecular biology to the diagnosis of genetic diseases, genetic predisposition and
molecular microbiology. I have worked as molecular- genetic analysis lab director. I
have 5 years’ experience in Medical Biopathology Laboratory (including
Microbiology, Biocemistry and Hematogy) and in the scientific medical methodology.
I have a great ability to read and evaluate any scientific research and literature. I am
also very experienced in the development and operation of a molecular biology
laboratory.
PROFESSIONAL EXPERIENCE
“Diagnosis” Medical Diagnostic Centre in Rethymnon (2011 - present)
Post:
 Head of Molecular Biology Department
 Collaborator - Assistant in Medical Laboratory (Clinical Microbiology /
Biochemistry and Semen analysis Laboratory)
Responsibilities:
 Launch of Department of Molecular Biology - Genetics (laboratory equipment
/ scientific research; methodology / promotional activities / information of doctors)
 Responsible in molecular laboratory tests analyses
 Scientific collaborator - assistant in Medical Laboratory (Clinical
Microbiology/ Biochemistry and Semen analysis Laboratory)
Achievements:
 Complete development and operation of the molecular biology Department
(economic and scientific study)
 Special emphasis on developing methodology to approach infertility issues
(new medical tests- information of infertile couples - evaluation of laboratory
analyzes)
2
Omnigen Biotechnological Applications SA (2009- 2010)
Private stem cell bank
Post: Head of Laboratory
Responsibilities:
 Responsible of laboratory analyses
 Responsible of implementing new molecular analysis services (HPV DNA
microarrays, HPV mRNA test)
 Responsible of laboratory equipment and cord blood stem cell
cryopreservation of samples (ISO 17025)
 Active participation in the development of new protocols and
implementation of new services
Achievements:
 Upgrading the company services by creating a molecular biology department
(HPV- DNA microarrays)
EDUCATION
 Doctor of Philosophy degree, PhD (2005- 2013) Grade: Excellent
University of Crete, School of Medicine in collaboration with the University of
Athens, School of Medicine, Medical genetics Laboratory - “Research Laboratory
Choremeio”
Dissertation Theme: "Molecular investigation of Angelman Syndrome patients.
Genotype - phenotype correlation”.
 Master of Science, M.Sc. (2003- 2005) Grade: Excellent
University of Crete School of Medicine
"Cellular and genetic etiology, diagnosis and treatment of human disease”.
Thematic postgraduate modules:
Molecular, genetic and cellular basis of human diseases / introduction to basic
laboratory techniques and clinical research methodologies / clinical genetics /
genetic epidemiology / immunology / cancer genetics / cellular aging.
Rotation projects:
 Laboratory of Human Molecular Genetics, Department of Biology University
of Crete
“Comparative study of the expression pattern of genes fra10ac1, sap145 and dgcr14”
 Laboratory of Clinical Hematology, School of Medicine, University of Crete
“Introduction to the methodology of cell culture”
MSc thesis: University of Athens, School of Medicine, Medical Genetics Laboratory -
"Choremeio Research Laboratory" Children's Hospital "Agia Sofia” - "Molecular
study of chromosomal region 15q11- q13. Methylation pattern analysis - Detection
of mutations in the UBE3A gene. "
3
 Biology BSc, Bachelor Degree (1999- 2003) Grade: Very Good
University of Athens, Faculty of Science, Department of Biology
Thesis: Medical School, University of Athens, Molecular Pathology Laboratory
“Molecular Study of soft tissue tumors”
LABORATORY EXPERIENCE
Molecular biology (DNA / RNA extraction / DNA Sequencing / PCR / MS-PCR / ECMA
/ SSCP / HPV DNA microarrays / In situ hybridization, Western blotting).
Medical Microbiology
Sperm Analysis (Sperm diagram, Sperm DNA Fragmentation Assay)
Clinical Biochemistry Laboratory
Clinical Hematology Laboratory
Cell cultures (peripheral blood lymphocyte / Mesenchymal stem cells of umbilical
cord)
Umbilical cord blood processing techniques (isolation of hematopoietic stem cells,
cryopreservation of hematopoietic and mesenchymal stem cells)
Flow Cytometry
Classical cytogenetic (G- banding).
PUBLICATIONS
1. Tzagkaraki Evmorfia, Sofocleous C., Frysira H., Dinopoulos A., Mavrou A.,
Kanavakis E. Novel human pathological mutations. Gene symbol UBE3A. Disease:
Angelman syndrome. Human Genet. 2009 Aug; 126(2): 331
2. Tzagkaraki E., Sofocleous C., Tounta G., Mavrou A., Kolialexi A. The role of Vitamin
C in human reproduction. Handbook of Vitamin C Research; daily requirements,
dietary sources and adverse effects. Nova Biomedical. Nova Publishers 2009
3. Tzagkaraki E, Sofocleous C, Fryssira-Kanioura H, Dinopoulos A, Goulielmos G,
Mavrou A, Kitsiou-Tzeli S, Kanavakis E. Screening of UBE3E gene in patients referred
for Angelman Syndrome. Eur J Paediatr Neurol. 2013 Jul; 17(4):366-73.
AWARDS
Poster award: 3rd
International Congress on Brain and Behavior and 16th
Thessaloniki Conference of the South- East European Society for Neurology and
Psychiatry (Dual Congress) , World Psychiatric Association (WPA)
“Molecular investigation of Angelman syndrome in Greece. Screening for UBE3A
mutations; preliminary results” E. Tzagkaraki1, 2
, C. Sofocleous1
, N. Vogiatzakis1
, S.
Kitsiou1
, H. Frysira1
, G. Goulielmos2
, E. Kanavakis1
, A. Mavrou1
1
University of Athens, School of Medicine, Athens, Greece, 2
University of Crete,
School of Medicine, Heraklion, Crete
4
PARTICIPATION IN SCIENTIFIC CONFERENCES
 3rd
INTERNATIONAL CONGRESS ON BRAIN AND BEHAVIOUR AND 16th
THESSALONIKH CONFERENCE OF THE SOUTH- EAST EUROPEAN SOCIETY FOR
NEUROLOGY AND PSYCHIATRY (WPA) 2007
Poster presentation “MOLECULAR INVESTIGATION OF ANGELMAN SYNDROME IN
GREECE. SCREENING FOR UBE3A MUTATIONS; PRELIMINARY RESULTS”
E. Tzagkaraki1, 2
, C. Sofocleous1
, N. Vogiatzakis1
, S. Kitsiou1
, H. Frysira1
, G.
Goulielmos2
, E. Kanavakis1
, A. Mavrou1
1
University of Athens, School of Medicine, Athens, Greece, 2
University of Crete,
School of Medicine, Heraklion, Crete
 4th ANNUAL SCIENTIFIC CONFERENCE OF MEDICAL SCHOOL OF THE
UNIVERSITY OF ATHENS 2007
Poster presentation: “MOLECULAR OF ANGELMAN/PRADER WILLI PATIENTS
SCREENING FOR UBE3A MUTATIONS, PRELIMINARY RESULTS”.
E. Tzagkaraki1, 2
, Ch. Sofocleous1
, S. Kitsiou1
, E. Frysira1
, G. Goulielmos2
, E. Kanavakis1
,
A. MaVrou1
1
University of Athens, School of Medicine, Athens, Greece, 2
University of Crete,
School of Medicine, Heraklion, Crete
 EUROPEAN HUMAN GENETICS CONFERENCE- EUROPEAN
SOCIETY OF HUMAN GENETICS (ESHG) 2007- NICE, FRANCE
Poster presentation “MOLECULAR INVESTIGATION OF ANGELMAN AND PRADER
WILLI SYNDROMES. SCREENING FOR UBE3A MUTATIONS. PRELIMINARY RESULTS”
E. Tzagkaraki1, 2
, C. Sofocleous1
, S. Kitsiou1
, H. Frysira1
, G. Goulielmos2
, E. Kanavakis1
,
A. Mavrou1
1
University of Athens, School of Medicine, Athens, Greece, 2
University of Crete,
School of Medicine, Heraklion, Crete
CONFERENCES
 Greek Society of Perinatal Medicine Conference on "Prenatal testing and fetal
medicine", Heraklion Crete, 2015
 Two days Conference of Medical Genetics Laboratory and A’ Obstetrics and
Gynaecology Clinic, Athens University Medical School: 20 Years of Preimplantation
Genetic Diagnosis, Athens 2010.
 XX International Congress of Genetics, International Genetics Federation
(IGF), Berlin Germany 2008
 3rd
International Congress on Brain and Behavior and 16th
Thessaloniki
Conference of the South- East European Society for Neurology and Psychiatry, World
Psychiatric Association (WPA) 2007
 20th
Course in Medical Genetics, European Genetics Foundation, Bologna
University Residential Centre, Bertinoro Di Romagna 2007
 7th
Course in Bioinformatics for Molecular Biologists, European Genetics
Foundation, Bologna University Residential Centre, Bertinoro Di Romagna 2007
5
 14th
National Congress of Perinatal Medicine, Greek Society of Perinatal
Medicine, Athens 2007
 6th
Post-graduate Genetic Seminar: Genetic in congenital heart disease,
Medical Genetic Laboratory, University of Athens, Athens 2006
 Educational Seminars of Research and Technology: Photonics Microscopy
Modern Methods and Applications in Biomedical Research and Diagnosis, Greek
Pasteur Institute, Athens 2005
 1st
Conference of the Medical Genetics Laboratory of Athens University,
Genetics and Infertility, Athens 2005
 12th
Euroconference on Apoptosis, Chania, Crete 2004
 2004 HDL Workshop, Heraklion, Crete 2004
 1st
International Biotechnology Conference in Greece, National Hellenic
Research Foundation, Athens 2004
 2nd
Seminar of Genetic Counselling and Prenatal Screening, Medical School,
University of Crete, Heraklion 2004
 2nd
International Conference of Functional Genomics of Ageing, Hersonissos,
Crete 2004
 25th
Conference of Greek Association for Biological Sciences, Athens 2003
 Educational lecture cycle at the National Research Foundation in Athens
Health and Society: Current Health Problems and their Treatment, Athens 2003
 4th
National Conference of Cancer Indicators, Athens 2002
FOREIGN LANGUAGES
English: Proficiency (Cambridge University)
German: Grundstufe (Goethe Institut)
Italian: Intermedio (Palso)
COMPUTER SKILLS
Ms Office (Word/ Excel/ PowerPoint)
Internet Explorer
REFERENCES
Available upon request
_________________________________
The undersigned, certified translator, do hereby certify that the foregoing is a true
translation of the attached Greek document

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ALEXANDER POLINKOVSKY CV 2015
 

EVMORFIA G TZAGKARAKI CVi

  • 1. 1 OFFICIAL TRANSLATION EVMORFIA G. TZAGKARAKI Molecular Biologist, M.Sc., PhD Mob. +30 697 4862 199 Email: etzagkaraki@outlook.com PERSONAL INFORMATION: Date of Birth: April 5, 1980 Nationality: Greek Marital status: Unmarried Permanent residence: Rethymnon, Crete, Greece Molecular biologist - Laboratory geneticist with 6 years’ experience in the application of molecular biology to the diagnosis of genetic diseases, genetic predisposition and molecular microbiology. I have worked as molecular- genetic analysis lab director. I have 5 years’ experience in Medical Biopathology Laboratory (including Microbiology, Biocemistry and Hematogy) and in the scientific medical methodology. I have a great ability to read and evaluate any scientific research and literature. I am also very experienced in the development and operation of a molecular biology laboratory. PROFESSIONAL EXPERIENCE “Diagnosis” Medical Diagnostic Centre in Rethymnon (2011 - present) Post:  Head of Molecular Biology Department  Collaborator - Assistant in Medical Laboratory (Clinical Microbiology / Biochemistry and Semen analysis Laboratory) Responsibilities:  Launch of Department of Molecular Biology - Genetics (laboratory equipment / scientific research; methodology / promotional activities / information of doctors)  Responsible in molecular laboratory tests analyses  Scientific collaborator - assistant in Medical Laboratory (Clinical Microbiology/ Biochemistry and Semen analysis Laboratory) Achievements:  Complete development and operation of the molecular biology Department (economic and scientific study)  Special emphasis on developing methodology to approach infertility issues (new medical tests- information of infertile couples - evaluation of laboratory analyzes)
  • 2. 2 Omnigen Biotechnological Applications SA (2009- 2010) Private stem cell bank Post: Head of Laboratory Responsibilities:  Responsible of laboratory analyses  Responsible of implementing new molecular analysis services (HPV DNA microarrays, HPV mRNA test)  Responsible of laboratory equipment and cord blood stem cell cryopreservation of samples (ISO 17025)  Active participation in the development of new protocols and implementation of new services Achievements:  Upgrading the company services by creating a molecular biology department (HPV- DNA microarrays) EDUCATION  Doctor of Philosophy degree, PhD (2005- 2013) Grade: Excellent University of Crete, School of Medicine in collaboration with the University of Athens, School of Medicine, Medical genetics Laboratory - “Research Laboratory Choremeio” Dissertation Theme: "Molecular investigation of Angelman Syndrome patients. Genotype - phenotype correlation”.  Master of Science, M.Sc. (2003- 2005) Grade: Excellent University of Crete School of Medicine "Cellular and genetic etiology, diagnosis and treatment of human disease”. Thematic postgraduate modules: Molecular, genetic and cellular basis of human diseases / introduction to basic laboratory techniques and clinical research methodologies / clinical genetics / genetic epidemiology / immunology / cancer genetics / cellular aging. Rotation projects:  Laboratory of Human Molecular Genetics, Department of Biology University of Crete “Comparative study of the expression pattern of genes fra10ac1, sap145 and dgcr14”  Laboratory of Clinical Hematology, School of Medicine, University of Crete “Introduction to the methodology of cell culture” MSc thesis: University of Athens, School of Medicine, Medical Genetics Laboratory - "Choremeio Research Laboratory" Children's Hospital "Agia Sofia” - "Molecular study of chromosomal region 15q11- q13. Methylation pattern analysis - Detection of mutations in the UBE3A gene. "
  • 3. 3  Biology BSc, Bachelor Degree (1999- 2003) Grade: Very Good University of Athens, Faculty of Science, Department of Biology Thesis: Medical School, University of Athens, Molecular Pathology Laboratory “Molecular Study of soft tissue tumors” LABORATORY EXPERIENCE Molecular biology (DNA / RNA extraction / DNA Sequencing / PCR / MS-PCR / ECMA / SSCP / HPV DNA microarrays / In situ hybridization, Western blotting). Medical Microbiology Sperm Analysis (Sperm diagram, Sperm DNA Fragmentation Assay) Clinical Biochemistry Laboratory Clinical Hematology Laboratory Cell cultures (peripheral blood lymphocyte / Mesenchymal stem cells of umbilical cord) Umbilical cord blood processing techniques (isolation of hematopoietic stem cells, cryopreservation of hematopoietic and mesenchymal stem cells) Flow Cytometry Classical cytogenetic (G- banding). PUBLICATIONS 1. Tzagkaraki Evmorfia, Sofocleous C., Frysira H., Dinopoulos A., Mavrou A., Kanavakis E. Novel human pathological mutations. Gene symbol UBE3A. Disease: Angelman syndrome. Human Genet. 2009 Aug; 126(2): 331 2. Tzagkaraki E., Sofocleous C., Tounta G., Mavrou A., Kolialexi A. The role of Vitamin C in human reproduction. Handbook of Vitamin C Research; daily requirements, dietary sources and adverse effects. Nova Biomedical. Nova Publishers 2009 3. Tzagkaraki E, Sofocleous C, Fryssira-Kanioura H, Dinopoulos A, Goulielmos G, Mavrou A, Kitsiou-Tzeli S, Kanavakis E. Screening of UBE3E gene in patients referred for Angelman Syndrome. Eur J Paediatr Neurol. 2013 Jul; 17(4):366-73. AWARDS Poster award: 3rd International Congress on Brain and Behavior and 16th Thessaloniki Conference of the South- East European Society for Neurology and Psychiatry (Dual Congress) , World Psychiatric Association (WPA) “Molecular investigation of Angelman syndrome in Greece. Screening for UBE3A mutations; preliminary results” E. Tzagkaraki1, 2 , C. Sofocleous1 , N. Vogiatzakis1 , S. Kitsiou1 , H. Frysira1 , G. Goulielmos2 , E. Kanavakis1 , A. Mavrou1 1 University of Athens, School of Medicine, Athens, Greece, 2 University of Crete, School of Medicine, Heraklion, Crete
  • 4. 4 PARTICIPATION IN SCIENTIFIC CONFERENCES  3rd INTERNATIONAL CONGRESS ON BRAIN AND BEHAVIOUR AND 16th THESSALONIKH CONFERENCE OF THE SOUTH- EAST EUROPEAN SOCIETY FOR NEUROLOGY AND PSYCHIATRY (WPA) 2007 Poster presentation “MOLECULAR INVESTIGATION OF ANGELMAN SYNDROME IN GREECE. SCREENING FOR UBE3A MUTATIONS; PRELIMINARY RESULTS” E. Tzagkaraki1, 2 , C. Sofocleous1 , N. Vogiatzakis1 , S. Kitsiou1 , H. Frysira1 , G. Goulielmos2 , E. Kanavakis1 , A. Mavrou1 1 University of Athens, School of Medicine, Athens, Greece, 2 University of Crete, School of Medicine, Heraklion, Crete  4th ANNUAL SCIENTIFIC CONFERENCE OF MEDICAL SCHOOL OF THE UNIVERSITY OF ATHENS 2007 Poster presentation: “MOLECULAR OF ANGELMAN/PRADER WILLI PATIENTS SCREENING FOR UBE3A MUTATIONS, PRELIMINARY RESULTS”. E. Tzagkaraki1, 2 , Ch. Sofocleous1 , S. Kitsiou1 , E. Frysira1 , G. Goulielmos2 , E. Kanavakis1 , A. MaVrou1 1 University of Athens, School of Medicine, Athens, Greece, 2 University of Crete, School of Medicine, Heraklion, Crete  EUROPEAN HUMAN GENETICS CONFERENCE- EUROPEAN SOCIETY OF HUMAN GENETICS (ESHG) 2007- NICE, FRANCE Poster presentation “MOLECULAR INVESTIGATION OF ANGELMAN AND PRADER WILLI SYNDROMES. SCREENING FOR UBE3A MUTATIONS. PRELIMINARY RESULTS” E. Tzagkaraki1, 2 , C. Sofocleous1 , S. Kitsiou1 , H. Frysira1 , G. Goulielmos2 , E. Kanavakis1 , A. Mavrou1 1 University of Athens, School of Medicine, Athens, Greece, 2 University of Crete, School of Medicine, Heraklion, Crete CONFERENCES  Greek Society of Perinatal Medicine Conference on "Prenatal testing and fetal medicine", Heraklion Crete, 2015  Two days Conference of Medical Genetics Laboratory and A’ Obstetrics and Gynaecology Clinic, Athens University Medical School: 20 Years of Preimplantation Genetic Diagnosis, Athens 2010.  XX International Congress of Genetics, International Genetics Federation (IGF), Berlin Germany 2008  3rd International Congress on Brain and Behavior and 16th Thessaloniki Conference of the South- East European Society for Neurology and Psychiatry, World Psychiatric Association (WPA) 2007  20th Course in Medical Genetics, European Genetics Foundation, Bologna University Residential Centre, Bertinoro Di Romagna 2007  7th Course in Bioinformatics for Molecular Biologists, European Genetics Foundation, Bologna University Residential Centre, Bertinoro Di Romagna 2007
  • 5. 5  14th National Congress of Perinatal Medicine, Greek Society of Perinatal Medicine, Athens 2007  6th Post-graduate Genetic Seminar: Genetic in congenital heart disease, Medical Genetic Laboratory, University of Athens, Athens 2006  Educational Seminars of Research and Technology: Photonics Microscopy Modern Methods and Applications in Biomedical Research and Diagnosis, Greek Pasteur Institute, Athens 2005  1st Conference of the Medical Genetics Laboratory of Athens University, Genetics and Infertility, Athens 2005  12th Euroconference on Apoptosis, Chania, Crete 2004  2004 HDL Workshop, Heraklion, Crete 2004  1st International Biotechnology Conference in Greece, National Hellenic Research Foundation, Athens 2004  2nd Seminar of Genetic Counselling and Prenatal Screening, Medical School, University of Crete, Heraklion 2004  2nd International Conference of Functional Genomics of Ageing, Hersonissos, Crete 2004  25th Conference of Greek Association for Biological Sciences, Athens 2003  Educational lecture cycle at the National Research Foundation in Athens Health and Society: Current Health Problems and their Treatment, Athens 2003  4th National Conference of Cancer Indicators, Athens 2002 FOREIGN LANGUAGES English: Proficiency (Cambridge University) German: Grundstufe (Goethe Institut) Italian: Intermedio (Palso) COMPUTER SKILLS Ms Office (Word/ Excel/ PowerPoint) Internet Explorer REFERENCES Available upon request _________________________________ The undersigned, certified translator, do hereby certify that the foregoing is a true translation of the attached Greek document