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Brian T. Freeman
7005 Ivandale Road, Independence, OH 44131 | (216) 470-4632 | bfreems@gmail.com
Summary of Qualifications
 6+ years graduate research experience focused on discovering the role of stem cell fusion
on reprogramming somatic cells both in vivo and in vitro with a focus on the implications
for cardiac regeneration including four primary author publications
 Multiple podium presentations at conferences including Biomedical Engineering Society
(BMES), the University of Minnesota Genomics Center Genofest, and the University of
Minnesota Genomics Center Single Cell Genomics Meeting
 Extensive experience with all aspects of mammalian and stem cell culture
 Transplanted mesenchymal stem/stromal cells (MSCs) to infarcted murine hearts via a
collagen patch and tracked the fusion of MSCs with murine cells as well as the affects of
cell fusion on cardiac function via echocardiography
 Utilized single-cell RNA-seq to demonstrate extensive transcriptional diversification of
stem cell-cardiomyocyte fusion products as well as multilineage priming of MSCs
 1.5 years of undergraduate research in a chemical engineering research lab including
successful completion of an honors thesis, “Drug Delivery to Wound Sites to Promote
Angiogenesis”
 Industrial experience in a research and development lab working on developing, testing and
optimizing the crystallization process of a liquid thickening agent
Skills Summary
 Extensive experience with human/mouse mesenchymal/multipotent stem/stromal cells
(MSCs) and cardiomyocyte handling and culture methods including co-culture and
differentiation techniques. Responsible for all aspects of cell culture
 Experience with next generation sequencing (single-cell RNA-seq) and bioinformatics
techniques in statistical analysis programs (R, SCDE) including principal component
analysis, differential gene expression, hierarchal clustering and gene ontology
 Experience with mouse animal model including breeding, genotyping, tissue procurement,
cryo/formalin fixation, microtome sectioning, immunohistochemistry, Cre/LoxP system, in
vivo illuminescence system (IVIS) imaging and analysis of luminescent data
 Experience with transfection techniques such as electroporation, viral transduction, and
lipid-mediated gene delivery in various cell types
 Experience with imaging techniques such as confocal microscopy, fluorescent microscopy,
SEM, multiphoton microscopy, time-lapse cell culture imaging and live animal imaging

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 Experience with many protein- and cell-based assays such as flow cytometry, qRT-PCR,
western blot, and immunocytochemistry
 Experience with industrial research techniques such as diffusion modeling, rheometry, pilot
plant testing, plasma coating, and contact angle determination
 Extensive experience with writing and publishing (4 first author papers), research design,
presenting, data analysis, critical and creative thinking, problem solving, and teamwork
 Very well-rounded, self-motivated and passionate with an eagerness to learn and adapt to a
dynamic environment
 Strong problem solving skills and ability to multitask and tackle tough challenges in fast-
paced environments, and manage/prioritize projects to ensure timely completion of
important milestones.
Education
Ph.D. Biomedical Engineering, (December 2015)
University of Wisconsin – Madison, College of Science and Engineering
 Dissertation: The role of stem cell fusion in (re)programming somatic cells both in vitro
and in vivo: implications for cardiac regeneration (Advisor: Brenda M. Ogle)
 GPA: 3.984/4.0
B.S. Chemical Engineering and Biomedical Engineering (Double Major) (2009)
Carnegie Mellon University, Carnegie Institute of Technology
 CIT Honors Diploma
 Overall GPA: 3.93/4.0
Research Experience
(2013 – present) University of Minnesota – Twin Cities, Dept. of Biomedical Engineering
 Ph.D. advisor recruited to Minnesota and I chose to continue my research with advisor
(2009 – 2013) University of Wisconsin – Madison, Dept. of Biomedical Engineering
PROJECT TITLE: The role of stem cell fusion in (re)programming somatic cells both in
vitro and in vivo: implications for cardiac regeneration
Advisor: Brenda M. Ogle
 Designed and analyzed data for cell fusion experiments
 Utilized in vivo imaging system (IVIS) to detect and track cell fusion in real time and on an
organism wide level after MSC cell transplantation to heart
 Discovered that MSC fusion with cells of the infracted heart hinders healing via decreased
vascularization and immune modulation
 Utilized single-cell RNA-seq and bioinformatic techniques to demonstrate extensive
transcriptional diversification of stem cell-cardiomyocyte hybrids after fusion
 Discovered that single-cell mouse MSCs are primed for multilineage differentiation even
when in an undifferentiated state via single-cell RNA-seq

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(2008 – 2009) University of Pittsburgh, Dept. of Chemical Engineering
Undergraduate Research Assistant
PROJECT TITLE: Drug Delivery to Wound Sites to Promote Angiogenesis
Advisor: Steven Little
 Synthesized cellulose hollow fibers to deliver growth factors to wound site
 Determined effective diffusivities for fiber types and growth factor sizes
 Developed computer model describing the release profiles of growth factors
(2007) University of Maryland, Dept. of Bioengineering
Research Experience for Undergrads (REU) Researcher
PROJECT TITLE: RNA interference affects on intervertebral disc degeneration
Advisor: Adam Hsieh
 Established and maintained a mammalian cell culture
 Transfected intervertebral disc cells with GFP for identification
Work Experience
(2008) Procter & Gamble, Fabrics and Homecare Department
Engineer Intern
PROJECT TITLE: Continuous Crystallization of Structuring with Thixcin (SWT)
Advisor: Shaffiq Jaffer
 Analyzed crystallization process parameters of SWT at microscopic level
 Investigated rheological effects of adding SWT to solutions
 Optimized SWT production process by reducing batch time in pilot plant
Publications
1. Freeman BT, Jung PJ, Ogle BM. Single-cell RNA-seq reveals activation of unique gene
groups as a consequence of stem cell-parenchymal cell fusion. Scientific Reports, 6, Article
number 23270. doi:10.1038/srep23270. 2016.
2. Freeman BT, Ogle BM. Viral-mediated fusion of mesenchymal stem cells with murine cells
of the infarcted heart hinders cardiac healing via decreased vascularization and immune
modulation. Scientific Reports, 6, Article number 20283. doi:10.1038/srep20283. 2016.
3. Gao L, Kupfer ME, Jung PJ, Zhang P, Yang L, Tran QT, Ajeti V, Freeman BT, Campagnola
PJ, Zhang J, Ogle BM. Fabrication of human cardiac tissue using hiPSC-derived cardiac cells
and 3D printing of high resolution, ECM-inspired scaffolds. Stem Cells Translational
Medicine. (Accepted)
4. Freeman BT, Jung PJ, Ogle BM. Single-cell RNA-seq of murine bone-marrow
mesenchymal stem cells reveals multilineage priming for differentiation. PLoS ONE, 10(9):
e0136199. doi:10.1371/journal.pone.0136199. 2015.
5. Freeman BT, Kouris NA, Ogle BM. Tracking fusion of human mesenchymal stem cells
following transplantation to the heart. Stem Cells Translational Medicine, 4 (6), 685-694.
2015.
 Selected as a featured article for Regenerative Medicine Weekly newsletter (2015)
 Highlighted in Circulation Research Editorial (2015;117:494-497)
6. Kouris NA, Schaefer JA, Hatta M, Freeman BT, Kamp TJ, Kawaoka Y, Ogle BM. Directed
Fusion of Mesenchymal Stem Cells with Cardiomyocytes via VSV-G Facilitates Stem Cell
Programming. Stem Cells International AI 414038. doi: 10.1155/2012/414038. 2012.

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7. Sprangers AJ, Freeman BT, Kouris NA, Ogle BM. A Cre-Lox P Recombination Approach
for the Detection of Cell Fusion In Vivo. Journal of Visualized Experiments (59), e3581,
2012.
8. Sprangers AJ, Freeman BT, Ogle BM. Electroporation Can Efficiently Transfect hESC-
Derived Mesenchymal Stem Cells without Inducing Differentiation. The Open Stem Cell
Journal 3: 62-66. 2011.
Oral Presentations
1. Freeman BT and Ogle BM, “Single cell RNA-seq of stem cell-cardiomyocyte fusion
products: (Re)programming and implications for cardiac regeneration,” University of
Minnesota Genomics Center Genofest, Cancer & Cardiovascular Research Building,
Minneapolis, MN, October 23rd, 2014.
2. Freeman BT and Ogle BM, “The role of stem cell fusion in (re)programming somatic cells,”
University of Minnesota Genomics Center Single Cell Genomics Meeting, Cancer &
Cardiovascular Research Building, Minneapolis, MN, June 12th, 2014.
3. Freeman BT and Ogle BM, “Viral fusogens to promote stem cell reprogramming via
fusion,” BMES National Meeting 2010, Austin TX, October 6-9th, 2010.
Poster Presentations
1. Freeman BT, Jung JP and Ogle BM, “Single-cell RNA-seq reveals rapid transcriptional
diversification following stem cell-parenchymal cell fusion,” 2015 Minnesota
Supercomputing Institute Research Exhibition, Minneapolis, MN, April 23rd, 2015.
2. Freeman BT and Ogle BM, “Single-cell RNA-seq of stem cell-cardiomyocyte fusion
products: (Re)programming and implications for cardiac regeneration,” 2nd ENG-SCI
Symposium The Heart of the matter: Where Stem Cells and Engineers Meet, Cancer &
Cardiovascular Research Building, Minneapolis, MN, October 10th, 2014.
3. Freeman BT and Ogle BM, “The role of stem cell fusion in (re)programming somatic cells
in vitro: implications for cardiac regeneration,” University of Minnesota Cardiovascular
Retreat, Cancer & Cardiovascular Research Building, Minneapolis, MN July 30th, 2014.
4. Freeman BT and Ogle BM, “Tracking fusion of human mesenchymal stem cells following
transplantation,” 8th Annual Wisconsin Stem Cell Symposium: Cell-Based Therapy for Heart
and Vascular Disease: Pathways to Clinic, BioPharmaceutical Technology Center,
Fitchburg, WI April 10th, 2013.
5. Freeman BT, Tengood JE, Wargos S and Little SR, “Drug delivery to promote angiogenesis
using engineered hollow fibers,” CMU Meeting of the Minds, Pittsburgh, PA, April 30th,
2009.
Research Support
1. National Science Foundation Graduate Research Fellowship (PI: Ogle, #2010105691,
6/1/2010 – 5/31/2013): The impact of ischemia on mesenchymal stem cell function in the
cardiac microenvironment
Awards and Honors
1. NSF Graduate Research Fellowship (2010), University of Wisconsin-Madison, Madison, WI

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2. Johnson & Johnson Undergraduate Research Award (2009), Carnegie Mellon University,
Pittsburgh, PA
3. NCAA Postgraduate Scholarship (2009), Carnegie Mellon University, Pittsburgh, PA
4. Draddy (Campbell) Award Finalist, National Football Foundation, National Scholar-Athlete
(2008), Carnegie Mellon University, Pittsburgh, PA
5. Andrew Carnegie Society (2008), Carnegie Mellon University, Pittsburgh, PA
6. Phi Kappa Phi Honor Society (2008), Carnegie Mellon University, Pittsburgh, PA
7. Tau Beta Pi, Engineering Honor Society (2008), Carnegie Mellon University, Pittsburgh, PA
8. ESPN the Magazine Academic All-American (Football) (2006-2008), Carnegie Mellon
University, Pittsburgh, PA
9. Carnegie Mellon Student Employee of the Year Finalist (2006-2008), Carnegie Mellon
University, Pittsburgh, PA
10. Carnegie Institute of Technology Dean’s List (2005-2009), Carnegie Mellon University,
Pittsburgh, PA
Teaching
Research Mentor
1. Anthony Sprangers – Undergraduate, University of Wisconsin (2010 – 2013)
2. Drew Birrenkott – Undergraduate, University of Wisconsin (2012 – 2013)
3. Tanner McArdle – Undergraduate, University of Minnesota-Twin Cities (2014-2015)
Teaching Experience
1. MedTrack Advisory Committee member, Designed a medical training curriculum for high
school students at Padua Franciscan High School, Parma, OH (2010-present)
2. UW-Madison DELTA (Teaching-as-research) course, EPD 654: Teaching in Science and
Engineering: The College Classroom (2011)
3. Guest Lecturer: AP Biology, AP Chemistry at Padua Franciscan High School, Parma, OH.
Introduced Biomedical Engineering and Stem Cell Research to high school students (2010)
4. Keynote Speaker: Medtrack Honors Advanced Science Seminar at Padua Franciscan High
School, Parma, OH. Presented thesis and research as a career path (2015)
5. Long-term substitute teacher for Honors and AP Chemistry at Padua Franciscan High
School, Parma, OH. (January 2016-March 2016)
Professional Memberships
1. Biomedical Engineering Society (2009 – present)
Activities
1. University of Wisconsin Biomedical Engineering Graduate Student Association (BME GSA)
Vice President 2010, President 2011-2013, Recruitment Chair 2011-13 (2009 – 2015)
2. MedTrack Advisory Board, Padua Franciscan High School, Parma, OH (2010 – Present)
3. University of Wisconsin Cheerleading, Madison, WI (2011 – 2013)
4. Student Athletic Advisory Council, Carnegie Mellon University, Pittsburgh, PA (2007–2009)
5. Carnegie Mellon University Football, Team Captain 2008, Pittsburgh, PA (2005 – 2009)

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References
Brenda M. Ogle, Ph.D., dissertation advisor
University of Minnesota-Twin Cities, Department of Biomedical Engineering
ogle@umn.edu
(612) 626-4468
Sean P. Palecek, Ph.D.
University of Wisconsin-Madison, Department of Chemical and Biological Engineering
palecek@engr.wisc.edu
(608) 262-8931
David J. Beebe, Ph.D.
University of Wisconsin-Madison, Department of Biomedical Engineering
djbeebe@wisc.edu
(608) 262-2260