Details about all type of culture media for growing the bacteria,
the basic constituents of culture media
types of media
simple media
special media
complex media
transport media
sugar media
anaerobic media
Nutritional requirements of bacteria and nutrient media (2) copyvinaya warad
To understand nutritional requirements of bacteria
To study nutritional classification of bacteria
To study constituents of nutrient media
To understand types of nutrient media.
To understand uses of different nutrient media
Details about all type of culture media for growing the bacteria,
the basic constituents of culture media
types of media
simple media
special media
complex media
transport media
sugar media
anaerobic media
Nutritional requirements of bacteria and nutrient media (2) copyvinaya warad
To understand nutritional requirements of bacteria
To study nutritional classification of bacteria
To study constituents of nutrient media
To understand types of nutrient media.
To understand uses of different nutrient media
Definition of bacterial growth
Modes of multiplication in bacteria
List the salient features of bacterial growth curve.
Concepts of generation time and growth curve
Calculations of generation time
Slides includes all details about Dark field Microscopy.
useful for MTech, pharmacy student which dealing with microbiology. also for reference to study Dark Field Microscopy. includes principle, instrumentation, working, uses etc.
a brief overview of endotoxin and exotoxin is given in the ppt. as for bachelor's level this would be sufficient to know about the toxins but as for master's and higher studies more in-depth level of understanding is required. Hope this helps everyone who is referring this.
Nutrient media – A source of amino acids and nitrogen (e.g., beef, yeast extract). This is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown
Definition of bacterial growth
Modes of multiplication in bacteria
List the salient features of bacterial growth curve.
Concepts of generation time and growth curve
Calculations of generation time
Slides includes all details about Dark field Microscopy.
useful for MTech, pharmacy student which dealing with microbiology. also for reference to study Dark Field Microscopy. includes principle, instrumentation, working, uses etc.
a brief overview of endotoxin and exotoxin is given in the ppt. as for bachelor's level this would be sufficient to know about the toxins but as for master's and higher studies more in-depth level of understanding is required. Hope this helps everyone who is referring this.
Nutrient media – A source of amino acids and nitrogen (e.g., beef, yeast extract). This is an undefined medium because the amino acid source contains a variety of compounds with the exact composition being unknown
Culture medium or growth medium is a liquid or gel designed to support the growth of microorganisms. There are different types of media suitable for growing different types of cells. Here, we will discuss microbiological cultures used for growing microbes, such as bacteria ,fungi, yeast & algae.
These slides explain how media preparation in microbiology lab
for bacterial culture, history of culture media, types of culture media based on concentration and ingredients and practical section.
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2. INTRODUCTION
• Culture media/ growth media are specific mixtures of nutrients and
other substances that support the growth of microorganisms.
• Some bacterial species are able to grow under wide range of
conditions, but others are selective.
• Artificial culture medium must provide all nutritional components that
a bacterium gets in its natural habitat.
• Louis Pasteur used simple broths made of urine and meat extracts.
• Robert Koch realized the importance of solid media and used potato
pieces to grow bacteria.
3. REASONS TO CULTIVATE
MICROORGANISMS IN PURE FORM
• To demonstrate their properties so as to identify them for their clinical
significance.
• To determine their sensitivity to antibiotics.
• To study their physiological virulence and genetic properties.
• For preparing antigens and vaccines.
• For epidemiological purposes.
• For research.
4. COMMON INGREDIENTS OF CULTURE
MEDIA
1. Peptone
2. Water
3. Agar
4. Inorganic salts
5. Growth factors
6. Blood
7. Serum
8. Casein hydrolysate
9. Meat extract
10. Yeast extract
11. Malt extract
6. CLASSIFICATION
BASED ON
CONSISTENCY
LIQUID MEDIA
SOLID MEDIA
SEMI-SOLID
MEDIA
BASED ON
CONSTITUENTS
SIMPLE MEDIA
COMPLEX
MEDIA
SYNTHETIC/
DEFINED MEDIA
BASED ON
FUNCTIONAL
REQUIREMENTS
ENRICHED
MEDIA
ENRICHMENT
MEDIA
SELECTIVE
MEDIA
INDICATOR
MEDIA
DIFFERENTIAL
MEDIA
TRANSPORT
MEDIA
ANAEROBIC
MEDIA
BASED ON
OXYGEN
REQUIREMENT
AEROBIC MEDIA
ANAEROBIC
MEDIA
7. BASED ON CONSISTENCY
1. LIQUID MEDIA
• Bacteria grow diffusely in liquids.
• Examples:
a) Peptone water
b) Nutrient broth
• Uses:
a) Used when large yields of bacteria are required.
b) For preparing bulk cultures of antigens and
vaccines.
c) Specimens containing antibiotics get neutralized
by dilution in the medium.
8. BASED ON CONSISTENCY
2. SOLID MEDIA
• Agar forms the base of solid media.
• 2% agar is used for solid media.
• Examples:
a) Nutrient agar
b) Blood agar
• Uses:
a) Bacteria have distinct colony morphology
and characteristic features on solid media,
which helps in identification.
9. BASED ON CONSISTENCY
3. SEMISOLID MEDIA
• Also called sloppy agar
• Concentration of agar is 0.2-0.5%.
• Examples:
a) Nutrient broth with 0.5% agar
• Uses:
a) To demonstrate motility of bacteria.
b) To maintain stock culture
10. BASED ON CONSTITUENTS
1. SIMPLE MEDIA
• They contain minimum ingredients that support growth of
non-fastidious bacteria.
• Examples:
a) Peptone water →1% peptone + 0.5% NaCl + water
b) Nutrient broth → peptone water + 1% meat extract
c) Nutrient agar → nutrient broth + 2% agar
11. • Uses of simple media:
a) Base for preparation of other
media.
b) Nutrient broth is used to study
bacterial growth curve.
c) Nutrient agar is used to
- study colony morphology.
- perform biochemical tests, like
oxidase, catalase test.
- demonstrate pigment producing
ability of bacteria
Nutrient broth
Nutrient agar
12. BASED ON CONSTITUENTS
2. COMPLEX MEDIA
• These have added ingredients for special
purposes.
• Examples:
a) MacConkey agar
b) Chocolate agar
• Uses:
a) Provides special nutrients required for
growth of bacteria under study.
Fig: Composition of
MacConkey agar
13. BASED ON CONSTITUENTS
3. SYNTHETIC / DEFINED MEDIA
• Made from pure chemical substances.
• Exact composition of medium is known.
• Examples:
a) MacConkey agar
b) Nutrient agar
• Uses:
a) For various special studies.
b) They are highly selective, sensitive and rapid.
14. BASED ON FUNCTIONAL REQUIREMENTS
1. ENRICHED MEDIA
• Nutritional requirements are added to basal
medium.
• Examples:
a) Blood agar
b) Chocolate agar
c) Brain-heart infusion broth (BHIB)
• Uses:
a) To cultivate exacting or fastidious bacteria.
Fig: Blood agar
16. 2. ENRICHMENT MEDIA
• Employed for specimens with mixed
flora
• Examples:
a) Tetrathionate broth
b) Selenite F broth
• Uses:
a) Suppress commensal bacteria
while allowing pathogen to remain
viable and to grow.
BASED ON FUNCTIONAL REQUIREMENTS
17. 3. SELECTIVE MEDIA
• Contains agents that inhibit the growth of all except that which is required.
• Examples:
a) Deoxycholate citrate agar(DCA) – Salmonella species
b) Thiosulphate citrate bile sucrose agar(TCBS) – Vibrio cholerae
c) Thayer-Martin medium – N. gonorrhoeae
d) Lowenstein-Jensen medium – Mycobacterium species
• Uses:
a) Subculture colony of interest onto agar that will inhibit growth of unwanted
colonies.
BASED ON FUNCTIONAL REQUIREMENTS
19. 4. INDICATOR MEDIA
• These medium contain an indicator that
changes color when bacterium grows on
it.
• Examples:
a) Wilson-Blair medium – S. typhi
b) McLeod’s medium/ Potassium tellurite
agar – Corynebacterium diphtheriae
c) TCBS agar (Sucrose)
d) MacConkey agar (Neutral red dye)
BASED ON FUNCTIONAL REQUIREMENTS
20. 5. DIFFERENTIAL MEDIA
• Contains factor(s) that allow certain organisms
to exhibit different metabolic characteristics.
• Examples:
a) MacConkey agar
b) Blood agar
c) Cysteine lactose electrolyte deficient
agar(CLED)
• Uses:
a) To isolate and enumerate bacteria
BASED ON FUNCTIONAL REQUIREMENTS
CLED AGAR
23. 6. TRANSPORT MEDIA
• Devised to transport specimens
suspected to have fastidious
organisms.
• Examples:
a) Stuart’s transport medium
b) Amies transport medium
c) Cary Blair medium
• Uses:
a) To transport specimens.
BASED ON FUNCTIONAL REQUIREMENTS
24. 7. ANAEROBIC MEDIA
• These media contain reducing agents
• Examples:
a) Robertson’s cooked meat medium
b) Thioglycolate medium
• Uses:
a) To cultivate anaerobic organisms like
Clostridia, Bacteroides.
BASED ON FUNCTIONAL REQUIREMENTS
ROBERTSON’S
COOKED MEAT MEDIA
