This document discusses the requirements and procedures for conducting in vitro studies according to Good Laboratory Practice (GLP). It outlines prerequisites like specialized infrastructure, training, and waste disposal arrangements. Test systems must be justified, characterized, and procured from verified vendors. Controls, equipment performance, and cell authenticity must be documented. Procedures must ensure sterility, prevent cross-contamination, and maintain separation between studies. Records must document materials, equipment calibration and maintenance, cell characterization and storage, and implementation of safety and management plans.