This document summarizes a student's biomedical research project on analyzing circulating tumor cells. The aims of the project were to become familiar with cancer research methods, learn about circulating tumor cells and their antigen determinants, and analyze quantification methods like microfiltration and immunofluorescence staining. The student describes protocols for culturing and manipulating cancer cells, including thawing, culturing, splitting, and freezing cells. Immunofluorescence staining techniques are explained, using primary and secondary antibodies to target cytokeratin and analyze co-expression with CD44 and DAPI. Results showed positive staining for cytokeratin and CD44 in cultured cancer cells. Laser capture microdissection was also mentioned.