Mycobacteriophages were isolated from soils in Puerto Rico and classified into clusters using PCR and gel electrophoresis. Phage DNA was amplified with cluster-specific primers and analyzed on gels. One phage, Phagus_Maximus, was classified as belonging to Cluster B2 based on a 500 base pair band. The clusters of other phages could not be determined due to ambiguous or absent bands, requiring new primers or DNA preparation. Classifying bacteriophages aids in understanding their evolution and biological processes.