This study characterized the 2-93 mutant in Arabidopsis thaliana, which enhances the auxin deficient phenotype of the wei8 mutant. The 2-93 mutant displayed reduced ethylene response and root meristem activity. Genetic analysis determined the 2-93 mutation was recessive. Marker analysis linked the causal mutation to chromosome 2, though further testing is needed. Characterization of the 2-93 mutant will advance understanding of auxin biosynthesis through the tryptophan-dependent pathway.