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CLEANING OF LABORATORY NEW
GLASSWARE
By: Rahul Gautam
M.Sc. Biochemistry
Certified MLT & Phlebotomy Trainer
OBJECTIVES
• PRINCIPLE
• REQUIREMENTS
• PROCEDURE
CLEANING OF LABORATORY NEW GLASSWARE
Principle : Lab glasswareis usuallymanufactured
fromborosilicateglass (hard glass).
It isresist tothe actionofchemicalswiththe exceptionof
hydrofluoric acidand phosphoric acid.
It can withstandmechanicalbreakageand sudden
change oftemperature.
Soda limeglassware(soft glass)is not as goodas
borosilicateglassware.
REQUIREMENTS
• Soda lime glassware ( soft glassware)
• Borosilicate glassware or hard glassware.
• 5% HCL
• Tap water
• Distilled water
• 2% detergent solution
PROCEDURE
FOR SODA LIME OR SOFT GLASSWARE
1. The free alkali present on soda lime glassware is neutralized
by treating overnight with 5% HCL
2. Rinse the glassware several times in tap water.
3.After rinsing with tap water rinse the glassware several
times in distilled water .
4. Dry the glassware in hot air oven
• Note : To test that the free alkali has been neutralized ,
Autoclave the glassware in neutral distilled water and when
cool check the pH of
water.
THANK YOU

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Cleaning of new glassware by rahul gautam

  • 1. CLEANING OF LABORATORY NEW GLASSWARE By: Rahul Gautam M.Sc. Biochemistry Certified MLT & Phlebotomy Trainer
  • 3. CLEANING OF LABORATORY NEW GLASSWARE Principle : Lab glasswareis usuallymanufactured fromborosilicateglass (hard glass). It isresist tothe actionofchemicalswiththe exceptionof hydrofluoric acidand phosphoric acid. It can withstandmechanicalbreakageand sudden change oftemperature. Soda limeglassware(soft glass)is not as goodas borosilicateglassware.
  • 4. REQUIREMENTS • Soda lime glassware ( soft glassware) • Borosilicate glassware or hard glassware. • 5% HCL • Tap water • Distilled water • 2% detergent solution
  • 5. PROCEDURE FOR SODA LIME OR SOFT GLASSWARE 1. The free alkali present on soda lime glassware is neutralized by treating overnight with 5% HCL
  • 6. 2. Rinse the glassware several times in tap water.
  • 7. 3.After rinsing with tap water rinse the glassware several times in distilled water .
  • 8. 4. Dry the glassware in hot air oven
  • 9. • Note : To test that the free alkali has been neutralized , Autoclave the glassware in neutral distilled water and when cool check the pH of water.