This is a work that is like a rehearsal or practice in the formation of proposals of science in the RISE program which I participate.

1. Proposal by: Luzmarie Reyes Vicente Characterization of the resistant patterns of escherichia coli O104:H4 poison food outbreak in Europe.

2. e. coli More than 700 serotypes of e. coli have been identified. The O and H antigens and flagella distinguish the different e. coli respectively. There are large groups of bacterial germs that inhabit the intestinal tract of humans. But most kinds of e. coli don’t cause disease in human indeed.

3. The way it functions that affect: The shiga toxins enters cells and stops the cells from producing in the needs to function. Without the ability to sustain its function and dyes with apoptosis. This process is known as retrograde transport because it fallows the route opposite of proteins produced by a cell. The toxins can cause a deliberating effect in body because of killing of cells crucial for organ factor. The name is derived from the shigelladysenteria.

4. Why they can be so dangerous? It seems likely that DNA from shiga toxin producing shigela bacteria was transferred by bacteriophage harmless e.colibacteria, thereby providing them with the genetic material to produce these toxins. With e. coli can cause hemorrhagic cotliticand post-diarrheal HUS hemolytic undermic syndrome.

5. Hypothesis: Obtain the pattern of susceptibility to antibiotics of bacteria e. coli O104:H4.

6. Article: Investigation update: outbreak of Shiga toxin producing e.coli O104 (STEC O10-H4)Infections associated travel to Germany. In June 22, 2011 there were 823 reported patients with HUS and 39 deaths. On June 10, 2011 it was announced that were contaminated row sprouts. There are no longer in restaurants or store schedule in Germany. Over the past week the number of cases associated with this brake marks has decreased.

7. A microbiological study made a characterization. they took 80 patience diagnosed and took samples. Screened with PCR to find the virulence of shiga-toxin-producing e.coli and measured adherence to epithelial cells to determine phylogeny and antimicrobial susceptibility.

8. They found that had a virulence profile that expressed a phenotype that defined shigatoxine-producing e. coli with agregativeadherence to epithelial cells and beta-lactamace.

9. Methodology: It will be taken a representative samples of different patients that present symptoms of HUS. The e. coli O104:H4 will be isolated. and goes to a dilution of the bacteria in normal saline in a concentration of 0.5 mac-farlan. This dilution will be exposed to different antibiotic families placed in a Petri muller-himpton.

10. Measure the halo of inhibition of growth. With this it will be determined if the bacteria is resistant ,sensible or intermediate in the presence of tested antibiotics.

11. List of tested antibiotics:

12. Objectives: Determine witch of the different antibiotic families can inhibit the development of the infected bacteria E. coli O104:H4.

13. Expected results: The creation of a antibiotic therapy design to be used as a treatment in the cases of people with this infection.

14. References: http://www.pasteur.edu.uy/publico/bonilla/Protocolos/Protocolos%20en%20inmuno/Electroforesis%20de%20proteinas.pdf (REPORT)http://www.cdc.gov/ecoli/ : Outbreak of Shiga toxin-producing E. coli O104 (STEC O104:H4) Infections Associated with Travel to Germany http://www.cdc.gov/ecoli/2011/ecoliO104/ http://learn.genetics.utah.edu/archive/conservation/tools/protein.html http://www.cdphe.state.co.us/dc/epidemiology/stec_fs.html http://www.thelancet.com/journals/laninf/article/PIIS1473-3099(11)70165-7/fulltext