PRESENTED TO:
DR. PARITOSH KUMAR BISWAS,
DEAN, FACULTY OF FOOD SCIENCES
AND TECHNOLOGY, CVASU.
2
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma
Proteobacteria
Order: Enterobacteriales
Family: Enterobacteriaceae
G...
German
Pediatrician
-studies intestinal
flora of infants
Bacterium coli
commune
"Escherichia coli"
are named after
him.
Dr...
E. coli is a gram-negative
bacteria
non-acid-fast
uniform staining
non-spore-forming bacillus
grows both aerobically ...
Brief Epidemiology
Shiga-toxin producing Escherichia coli (STEC) is a
zoonotic pathogen.
It causes diarrheal disease in ...
7
Pathotypes of E. coli
1. Enterotoxigenic E. coli( ETEC)
2. Enteropathogenic E. coli (EPEC)
3. Enteroinvasive E. coli (EI...
8
Pathogenecity:
Enterotoxigenic E. coli
Heat labile toxin
like choleragen
Adenyl cyclase
activated
cyclic AMP
secretion
w...
9
Antigenic Properties
1.O (somatic antigen) : 173 serotypes
2.K (capsular antigen) : 74 serotypes
3.H (flagellar antigen)...
10
MAJOR CLINICAL AND POSTMORTEM FINDINGS
1.Coligranuloma
2.Colisepticamia
3.Air sac disease
4.Avian cellulitis
5.Swollen ...
11
PICTURE: COURTESY OF PROF. DR. MASUDUZZAMAN, DPP,
CVASU.
pericarditis
perihepatitis
Airsaculitis
PICTURE: COURTESY OF P...
12
Extreme
perihepatitis
13
Egg in Uterus
Egg peritonitis
14
Yolk sac infection
15
Sample for diagnosis………….
Depends on where the lesions are located.
But liver, heart are the major samples,
In case of ...
16
Continued………….
E. Coli can not be normally grown in colony in blood
agar because it contains protease.
E. Coli is teste...
17
Prevention and control:
Vaccine 02K1,078K18
Inactivated vaccine
Multivalent vaccine made from pilli.
A live vaccine pre...
18
Karimi, V. et al (2011) reported that, The significant increase in the incidence of
resistance against antibiotics in t...
19
THANKS FOR HAVING
PATIENCE.
E. coli diagnosis in broiler and layer bird.
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E. coli diagnosis in broiler and layer bird.

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Hi,this is Abdullah Al Masud's presentation.Microbiological and pathological characteristics of E. coli in birds has shown here. It was made as assignment on my masters work in microbiology.

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E. coli diagnosis in broiler and layer bird.

  1. 1. PRESENTED TO: DR. PARITOSH KUMAR BISWAS, DEAN, FACULTY OF FOOD SCIENCES AND TECHNOLOGY, CVASU. 2
  2. 2. Kingdom: Bacteria Phylum: Proteobacteria Class: Gamma Proteobacteria Order: Enterobacteriales Family: Enterobacteriaceae Genus: Escherichia Species: Escherichia coli (E. coli) E. coli 3
  3. 3. German Pediatrician -studies intestinal flora of infants Bacterium coli commune "Escherichia coli" are named after him. Dr. Theodore von Escherich(1857-1911) 4
  4. 4. E. coli is a gram-negative bacteria non-acid-fast uniform staining non-spore-forming bacillus grows both aerobically and anaerobically Rod shaped bacterium. Many strains are motile peritrichous flagella ESCHERICHIA COLI: BASIC FEATURES 5 2.0-6.0 μm long and 1.1μm in diameter
  5. 5. Brief Epidemiology Shiga-toxin producing Escherichia coli (STEC) is a zoonotic pathogen. It causes diarrheal disease in humans and is of public health concern because of its ability to cause outbreaks. Severe disease such as hemorrhagic colitis (HC) or hemolytic-uremic syndrome (HUS) . E. coli O157:H7 is the most prevalent STEC serotype. Causes secondary diseases when host defense cell are being impaired. E. Coli 0157:H7 present enterohemorrhagic pathogen of human which can also be decolonized in chicken. 6
  6. 6. 7 Pathotypes of E. coli 1. Enterotoxigenic E. coli( ETEC) 2. Enteropathogenic E. coli (EPEC) 3. Enteroinvasive E. coli (EIEC) 4. Enterohemorrhagic E. coli (EHEC) 5. Enteroaggregative E. coli (EAEC)
  7. 7. 8 Pathogenecity: Enterotoxigenic E. coli Heat labile toxin like choleragen Adenyl cyclase activated cyclic AMP secretion water/ions Heat stable toxin cyclase activated cyclic GMP uptake water/ions Enteropathogenic E. Coli Intimin an adhesin Bind to intestinal cell Causes rearrangement of actin in host cell Attachment and effacement Changes in ultrastructure of intestinal cells Prime cause of diaarrhoea affected with EPEC
  8. 8. 9 Antigenic Properties 1.O (somatic antigen) : 173 serotypes 2.K (capsular antigen) : 74 serotypes 3.H (flagellar antigen) : 53 serotypes 4.F (fimbrial antigen) : 70 serotypes O1, O2, O35, 078 serotypes are more prevalent in poultry to cause diseases(O1K1 and 02K1 cause severe disease). O antigen polysaccharide phospholipid in nature, can be produced by boiling of culture at 100 c for 1 hour. There is 5 polyvalent antiserum at market, after grouping by polyvalent serum, further serogroup test is done by monovalent antiserum. K antigen generate after heating at 100 c for 1 hour or 121 c for 2.5 hour, are subdivided into L,A,N,B forms. O and K is important.
  9. 9. 10 MAJOR CLINICAL AND POSTMORTEM FINDINGS 1.Coligranuloma 2.Colisepticamia 3.Air sac disease 4.Avian cellulitis 5.Swollen head syndrome 6.Perihepatitis 7.Pericardits 8.Panopthalmitis 9.Yolk sac infection
  10. 10. 11 PICTURE: COURTESY OF PROF. DR. MASUDUZZAMAN, DPP, CVASU. pericarditis perihepatitis Airsaculitis PICTURE: COURTESY OF PROF. DR. MASUDUZZAMAN, CVASU.
  11. 11. 12 Extreme perihepatitis
  12. 12. 13 Egg in Uterus Egg peritonitis
  13. 13. 14 Yolk sac infection
  14. 14. 15 Sample for diagnosis…………. Depends on where the lesions are located. But liver, heart are the major samples, In case of septicemia and osteomyelitis bone marrow is the sample. Confirmatory and differential diagnosis FIG.: E. M.B agar(colonies have a metallic sheen. FIG: Mac Conkey agar(Large pink color colony of E. coli) Fig. E. coli on nutrient agar( Smooth, colorless, circular colony
  15. 15. 16 Continued…………. E. Coli can not be normally grown in colony in blood agar because it contains protease. E. Coli is tested positive in lactose fermentation test and consequently E. coli is IMVic test positive which is done after finding of typical growth. Growth in nutrient slant agar taken by scraping and PBS added Heated upto 100 c for 1 hour or 121 c for 2.5 hours For detection of O and K antigen Other detection methods PCR MLST PFGE
  16. 16. 17 Prevention and control: Vaccine 02K1,078K18 Inactivated vaccine Multivalent vaccine made from pilli. A live vaccine prepare form naturally occuring non pathogenic piliated strain Bt7 was effacious when used in chicken Best way- Biosecurity and improving hygienic management. Fecal contamination of water, feed must be avoided. Hatching egg must be kept free from fecal contamination.
  17. 17. 18 Karimi, V. et al (2011) reported that, The significant increase in the incidence of resistance against antibiotics in the E. coli strains isolated from broiler chickens is probably due to the increased use of antibiotics as feed additives for growth promotion and prevention of diseases, use of inappropriate antibiotics for treatment of diseases, resistance transfer among different between antibiotics used in poultry. Lutful Kabir, S. M(2010) reported that, Avian colibacillosis and salmonellosis are considered to be the major bacterial disease problems in the poultry industry world- wide and these diseases constitute a major public health burden and represent a significant cost in many countries. The economic and public health burden of these diseases have made this topic time demanding. Matthijs, M.G.R(1973) reported that, More insight in the pathogenesis of a superinfection of E. coli in IBV-infected broilers and in the underlying mechanisms of the increased susceptibility to colibacillosis might lead to new methods in prevention or reduction of colibacillosis. HAMMOUDI, A. and AGGAD*,H. (2008) reported that, Antibiotic resistances are frequently encoded by conjugative plasmids or transposons, thus E. coli of avian origin could act as a possible source for the transfer of antibiotic resistances to other bacterial species including human pathogens.
  18. 18. 19 THANKS FOR HAVING PATIENCE.

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