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Chapter II  Episode III
Proteins: a closer look  Structural Levels of Proteins Primary Structure-  The Amino Acid sequence of the peptide chain, held together by covalent bonds Secondary Structure-  Alpha Helix’s and Beta sheets, held together by hydrogen bonds Tertiary Structure –  3D structure of a SINGLE protein molecule, completely compacted and folded polypeptide chain, held together by Disulfide bonds and Ionic bonds Quaternary Structure-  A Complex built of several protein molecules or polypeptide chains (sub-units), bound together by Covalent bonds and Disulfide bridges
Proteins: Denaturing  What is  Denaturing ?  Simply put it is when a Protein Complex comes apart due to  acid, alkali or heat  Ex. Cooking an  EGG What  happens  to a Protein that is Denatured?  Biologically:  its original properties are  diminished  or rendered  completely useless Physically:  It loses it’s “useful” shape. i.e. It  ~unravels~
Proteins: Enzymes  Another word for Enzyme is  CATALYST ,[object Object],[object Object],[object Object],[object Object],Denoted with the suffix  “-ASE” Ex:  Lipase  (enzymes that catalyze the hydrolysis of fats into glycerol and fatty acids.
Proteins: Enzymes  Many Enzymes/Catalysts must be “ACTIVATED” before they can be useful Ex.  Phosphorylation ( Meaning simply:  The addition of  phosphate  to an organic compound .) The  “Active Site”  is part on the enzyme that the substrate binds to.   There’s WAY more to enzymes than this but… We’ll Keep it  Simple ACTIVE ENZYME~POOF~
Nucleic Acids  Moving on … I’m Excited…Aren’t you? ~oh yes~
Nucleic Acid  ,[object Object],[object Object],[object Object],[object Object],Make up what we know as  DNA   The “Blueprint” of an organism.  Also make up  RNA
Nucleic Acids: RNA vs. DNA What's the difference? RNA DNA Contains the Sugar  RIBOSE Single  Stranded  Uses the Nitrogen-containing Base  URACIL   Located  outside  the NUCLEUS Contains the Sugar  DEOXYRIBOSE Double  Stranded =  Double Helix ** Remember Watson and Crick? Uses the Nitrogen-containing Base  THYMINE Located  inside  the  NUCLEUS
Nucleic Acid: RNA vs. DNA What they have in  COMMON ? A Phosphate Group The Nitrogen-containing bases: Adenine, Guanine, Cytosine
Nucleic Acid: RNA vs. DNA So what matches with what? RNA DNA A =  U U  = A G = C C = G A =  T T  = A G = C C = G
Complete the DNA A--- T-- G-- C-- G-- G-- T-- T-- T-- --T --A --C --C --A --A --A --C --G What Do they mean? A T C G Adenine  Thymine Cytosine Guanine
Complete the RNA A--- U-- G-- C-- G-- G-- U-- U-- U-- --U --A --C --C --A --A --A --C --G What Do they mean? A U C G Adenine  Uracil Cytosine Guanine
ATP Adenosine Triphosphate Moving on…
ATP: Structure Kind of looks like something familiar doesn’t it? RNA? Interesting…
ATP: What’s so special? The High Energy bond of the  PHOSPHATE Group  at the tail end of the molecule  It powers most everything in the body
So what do you learn?  ,[object Object],[object Object],[object Object],[object Object],4 Primary, Secondary, Tertiary and Quaternary Amino Acids  Catalyst  Lowers it
So what did you learn? ,[object Object],[object Object],[object Object],[object Object],When a Proteins structure comes apart Heat, Acid or Alkali  It becomes less effective or even useless It unravels or loses it useful shape
So what did you learn? ,[object Object],[object Object],[object Object],[object Object],An Enzyme Nothing…it remains the same  Shape and charge of the Substrate Phosphorylation
So what did you learn? ,[object Object],[object Object],[object Object],[object Object],[object Object],The Area on a Enzyme that the Substrate binds to.  Phosphate Group, A Sugar, Nitrogenous Base Deoxyribose Ribose A Single Strand
So what did you learn?  ,[object Object],Double stranded = Double helix ,[object Object],Uracil ,[object Object],[object Object],A, T, G, C = Adenine, Thymine, Guanine, Cytosine A, U, G, C = Adenine, Uracil, Guanine, Cytosine
So what did you learn?  Where in the cell is RNA  found?  Where in the cell is DNA  found?  In RNA what matches with A (Adenine)? In DNA what matches with A (Adenine)? Outside the Nucleus Inside the Nucleus Uracil  Thymine
So what did you learn? What is does  ATP  stand for? Adenosine Triphosphate What is it about ATP that makes it the Power source for the body? The High Energy Bonds found in the Phosphate Chain If you were able to answer all of these from memory you’re a  WARRIOR . If  NOT ? Then study your notes again  Thursday We REVIEW

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Chapter ii (proteins nucleic acids and atp)

  • 1. Chapter II Episode III
  • 2. Proteins: a closer look Structural Levels of Proteins Primary Structure- The Amino Acid sequence of the peptide chain, held together by covalent bonds Secondary Structure- Alpha Helix’s and Beta sheets, held together by hydrogen bonds Tertiary Structure – 3D structure of a SINGLE protein molecule, completely compacted and folded polypeptide chain, held together by Disulfide bonds and Ionic bonds Quaternary Structure- A Complex built of several protein molecules or polypeptide chains (sub-units), bound together by Covalent bonds and Disulfide bridges
  • 3. Proteins: Denaturing What is Denaturing ? Simply put it is when a Protein Complex comes apart due to acid, alkali or heat Ex. Cooking an EGG What happens to a Protein that is Denatured? Biologically: its original properties are diminished or rendered completely useless Physically: It loses it’s “useful” shape. i.e. It ~unravels~
  • 4.
  • 5. Proteins: Enzymes Many Enzymes/Catalysts must be “ACTIVATED” before they can be useful Ex. Phosphorylation ( Meaning simply: The addition of phosphate to an organic compound .) The “Active Site” is part on the enzyme that the substrate binds to. There’s WAY more to enzymes than this but… We’ll Keep it Simple ACTIVE ENZYME~POOF~
  • 6. Nucleic Acids Moving on … I’m Excited…Aren’t you? ~oh yes~
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  • 8. Nucleic Acids: RNA vs. DNA What's the difference? RNA DNA Contains the Sugar RIBOSE Single Stranded Uses the Nitrogen-containing Base URACIL Located outside the NUCLEUS Contains the Sugar DEOXYRIBOSE Double Stranded = Double Helix ** Remember Watson and Crick? Uses the Nitrogen-containing Base THYMINE Located inside the NUCLEUS
  • 9. Nucleic Acid: RNA vs. DNA What they have in COMMON ? A Phosphate Group The Nitrogen-containing bases: Adenine, Guanine, Cytosine
  • 10. Nucleic Acid: RNA vs. DNA So what matches with what? RNA DNA A = U U = A G = C C = G A = T T = A G = C C = G
  • 11. Complete the DNA A--- T-- G-- C-- G-- G-- T-- T-- T-- --T --A --C --C --A --A --A --C --G What Do they mean? A T C G Adenine Thymine Cytosine Guanine
  • 12. Complete the RNA A--- U-- G-- C-- G-- G-- U-- U-- U-- --U --A --C --C --A --A --A --C --G What Do they mean? A U C G Adenine Uracil Cytosine Guanine
  • 14. ATP: Structure Kind of looks like something familiar doesn’t it? RNA? Interesting…
  • 15. ATP: What’s so special? The High Energy bond of the PHOSPHATE Group at the tail end of the molecule It powers most everything in the body
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  • 21. So what did you learn? Where in the cell is RNA found? Where in the cell is DNA found? In RNA what matches with A (Adenine)? In DNA what matches with A (Adenine)? Outside the Nucleus Inside the Nucleus Uracil Thymine
  • 22. So what did you learn? What is does ATP stand for? Adenosine Triphosphate What is it about ATP that makes it the Power source for the body? The High Energy Bonds found in the Phosphate Chain If you were able to answer all of these from memory you’re a WARRIOR . If NOT ? Then study your notes again Thursday We REVIEW