The attached narrated power point presentation attempts to explain the working principle of lasers as sources for optical communications. The material will be useful for KTU final year B Tech students who prepare for the subject EC 405, Optical Communications.
The attached narrated power point presentation attempts to explain the working principle of lasers as sources for optical communications. The material will be useful for KTU final year B Tech students who prepare for the subject EC 405, Optical Communications.
How to find the verdet's constant. This presentation discovers the basic concept behind the experiment of determing the verdet's constant for different substances
The attached narrated power point presentation attempts to explore the various semiconductor injection laser diode structures. The material will be useful for KTU final year B tech students who prepare for the subject EC 405, Optical Communications.
The three terminals of the FET are known as Gate, Drain, and Source.
It is a voltage controlled device, where the input voltage controls by the output current.
In FET current used to flow between the drain and the source terminal. And this current can be controlled by applying the voltage between the gate and the source terminal.
So this applied voltage generate the electric field within the device and by controlling these electric field we can control the flow of current through the device.
This presentation contains basics of Magnetic circuits such as, magnetic flux, flux density, MMF, Reluctance, Magnetization Curve(B-H Curve), Difference between Electric & Magnetic Circuit, Series & Parallel magnetic Circuits etc.
How to find the verdet's constant. This presentation discovers the basic concept behind the experiment of determing the verdet's constant for different substances
The attached narrated power point presentation attempts to explore the various semiconductor injection laser diode structures. The material will be useful for KTU final year B tech students who prepare for the subject EC 405, Optical Communications.
The three terminals of the FET are known as Gate, Drain, and Source.
It is a voltage controlled device, where the input voltage controls by the output current.
In FET current used to flow between the drain and the source terminal. And this current can be controlled by applying the voltage between the gate and the source terminal.
So this applied voltage generate the electric field within the device and by controlling these electric field we can control the flow of current through the device.
This presentation contains basics of Magnetic circuits such as, magnetic flux, flux density, MMF, Reluctance, Magnetization Curve(B-H Curve), Difference between Electric & Magnetic Circuit, Series & Parallel magnetic Circuits etc.
Chapter 1: THE ATOM MODEL :
Text book...An introduction to Atomic, Molecular Physics and LASER by Education Publishers, Aurangabad is useful for Physics students.
A laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation. The term "laser" originated as an acronym for "light amplification by stimulated emission of radiation"
Lasers have many important applications. They are used in common consumer devices such as DVD players, laser printers, and barcode scanners. They are used in medicine for laser surgery and various skin treatments, and in industry for cutting and welding materials. They are used in military and law enforcement devices for marking targets and measuring range and speed. Laser lighting displays use laser light as an entertainment medium. Lasers also have many important applications in scientific research
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
3. Resonant Cavity
• A radio-frequency oscillator consists of an amplifier, a
tuned circuit and a feedback mechanism.
– The feedback connects the amplifier output to its input, causing
the signal to increase as it periodically passes through the
amplifier.
– A steady state is reached when the system losses are exactly
made up by the gain through the amplifier.
– System losses constitute of power extracted from the oscillator
as useful output & heating loss.
– The tuned circuit determines the oscillation frequency.
4. Optical resonant cavity
• A laser is a Very-High-Frequency oscillator
– Also refer to an optic oscillator
• The laser consists of a cylindrically shaped medium with
mirrors attached at each end.
– The medium provides the amplification
– Properties of the medium determine the output frequency and
spectral width of the laser
• Mirrors provide feedback for the light oscillator,
reflecting the light back & forth through the amplification
medium.
• Power exits the laser through one of the mirrors, which is
partially transmitting.
5. Fig.A: A laser cavity consists of an amplifying
medium and optical cavity
6. Fabry-Perot resonator
• The two mirrors form a cavity called Fabry-Perot resonator
– In which two wave exist, one moving to the right and one moving
to the left
– The total field in the cavity is the sum of the two moving waves.
– This results in the standing-wave pattern
• To produce standing-wave pattern, the cavity must be an
integral number of half wavelength long, that is L = ml/2.
– where l is the wavelength as measured in the material within the
cavity and m is a positive integer.
7. Fig. B: Stationary Standing-wave
pattern
L = l/2
L = 2l/2
L = 3l/2
L = 4l/2
L = 4l/2
8. Cavity resonant frequencies
• Only wavelengths satisfying l=2L/m can exist inside the
cavity in a steady state.
– Any wave of another length interferes destructively with itself &
attenuates very quickly
– We say that the cavity is resonant at wavelength satisfying
l=2L/m.
• The resonant frequencies are found as
f = mc/2nL
• The longitudinal modes of the cavity is shown in Fig. C
– The spacing between adjacent cavity longitudinal mode is
D f = c/2nL
9. Fig. C: Allowed modes and their frequency due to stationary EM waves
within the optical cavity.
f
Allowed Oscillations (Cavity Modes)
L
Stationary EM oscillations
Mirror
Mirror
Dfc =c/2nL
fm–1 fm fm+1 fm+2 …
… fm+3
10. Stimulated emission and photon amplification
• An electron in an atom can be excited from an energy
level E1 to a higher energy level E2 by the absorption of
a photon energy
hu= E2 – E1
• When an electron at a higher energy level transits
down in energy to an unoccupied energy level, it emit a
photon
• There are two possibilities for the emission process
1. The electron undergo the downward transition by itself
spontaneously
2. It can be induced to do so by another photon
11. Spontaneous emission
• The electron falls down in energy from level
E2 to E1
– emits a photon of energy hu = E2–E1 in a random
direction as shown in Fig.1
– A random photon is emitted
• The transition is spontaneous provided that
the state with energy E1 is not occupied
• The emission process during the transition of
electron from E2 to E1 can be thought of as if
the electron is oscillating with a frequency u.
13. Stimulated emission
• An incoming photon of energy hu = E2 – E1
stimulates the whole emission process by
inducing the electron at E2 to transit down to E1
as shown in Fig.1
– The emitted photon is in phase with the incoming
photon
– It is in the same direction, it has the same
polarization and it has the same energy since hu =
E2–E1
14. Stimulated emission, cont
• During stimulate emission, the E-field of
incoming photon couples to the electron and
drives it with the same frequency as the photon
– The forced oscillation of the electron at a frequency
u = (E2–E1)/h causes it to emit EM radiation whose
E-field is in total phase with that of stimulating
photon.
– When the incoming photon leaves the site, the
electron return to E1because it has emitted a photon
of energy hu = E2–E1
15. Population Inversion
• Stimulated emission is the basis for obtaining
photon amplification
– since one incoming photon results in two
outgoing photons which are in phase.
– The incoming photon should not be absorbed by
another atom at E1.
• When we are considering a collection of atoms
to amplify the light, we must have the majority
of the atoms at the energy level E2
– When there are more atoms at E2 than at E1, we
then have what is called a population inversion
16. Optical pumping and stimulated
emission
• For three energy level system
– An external excitation causes the atoms in this system to be
excited to E3, which is called optical pumping
– From E3, the atoms decay rapidly to an energy level E2
• The state E2 is a long-lived state
– Since the atoms cannot decay rapidly from E2 to E1, they
accumulate at this energy level causing a population inversion
between E2 and E1
– When one atom at E2 decays spontaneously, it emits a photon
which can go on to a neighboring atom and cause that to execute
stimulated emission
– The photons from the latter can go on to the next atom at E2 and
cause that to emit by stimulated emission & so on.
– The result is an avalanche effect of stimulated emission
processes with all the photons in phase.
18. Light Amplification by Stimulated Emission of
Radiation
• At the end of the avalanche of stimulated
emission processes, the atoms at E2 would
have dropped to E1
– It can be pumped again to repeat the stimulated
emission cycle again
• The emission from E2 to E1 is called the lasing
emission
– The system we have just described for photon
amplification is a LASER, an acronym for
“Light Amplification by Stimulated Emission of
Radiation”
20. Upward transition rate
• Consider a medium as in Fig 1
– N1 atoms per unit volume with energy E1
– N2 atoms per unit volume with energy E2
• The rate of upward transition from E1 to E2by photon
absorption will be proportional to
– The number of atoms N1
– The number of photon per unit volume with energy hu = E2–E1.
Upward transition rate: R12 = B12 N1 r (hu) (1)
where B12 is a proportionality constant (Einstein coefficient)
r (hu) is the photon energy density per unit frequency
which represents the number of photon per unit volume with
an energy hu
22. Downward transition rate
• The rate of downward transitions from E2 to E1 involved
spontaneous and stimulated emission depends on
– The concentration of N2 of atoms at E2
– Both N2 and the photon concentration r (hu) with energy hu (=
E2–E1)
Downward transition rate:
R21 = A21 N2 + B21 N2 r (hu) (2)
– First term is due to spontaneous emission (no need
photon to drive it)
– Second term is due to stimulated emission which
requires photons to drive it.
where A21 & B12 are the Einstein coefficients for spontaneous
and stimulated emission respectively
23. Thermal Equilibrium
• To find the coefficients A21 , B12 , B21 , we consider the medium in
thermal equilibrium
• There is no net change with time in the populations at E1 and E2 which
means
R12 = R21 (3)
• In thermal equilibrium, Boltzmann statistics demands that
(4)
where kB is the Boltzmann constant & T is the absolute temperature
• In thermal equilibrium, radiation from the atom must give rise to an
equilibrium photon energy density that is given by Planck’s black body
radiation distribution law,
( )
T
k
E
E
N
N
B
1
2
1
2
exp
( ) )
5
(
1
exp
8
3
3
T
k
h
c
h
h
B
eq
u
u
u
r
24. Stimulated & spontaneous
( )
( )
( )
( ) ( ) ( )
( )
( ) 1
2
12
21
3
3
21
21
2
21
2
21
21
21
3
3
21
21
21
12
absorp
stim
is
absorption
o
emission t
stimulated
of
ratio
the
addition,
In
8
spon
stim
emission
s
spontaneou
to
stimulated
of
ratio
he
consider t
Now
/
8
/
and
that
shows
it
(5),
to
eqn(1)
From
larger.
much
is
it
fact
in
eqn(5);
by
described
not
is
course,
of
operation,
laser
the
During
ts.
coefficien
Einstein
the
determine
to
condition
this
using
are
we
m;
equilibriu
in thermal
only
applies
eqn(5)
in
Law
s
Planck'
that the
emphasize
to
important
is
It
N
N
R
R
h
h
c
A
h
B
N
A
h
N
B
R
R
c
h
B
A
B
B
h
u
r
u
u
r
u
r
u
u
r
25. Conclusion: stimulated emission
• There are two important conclusion
1. For stimulated photon emission to exceed photon
absorption, we need to achieve population
inversion, that is N2 > N1.
• According to Boltzmann statistics, N2 > N1 implies a
negative absolute temperature
• The laser principle is based on non-thermal equilibrium
2. For stimulated emission far exceed spontaneous
emission, we must have a large photon
concentration, which is achieved by building an
optical cavity to contain the photons
28. Principle of the Laser Diode
• Consider a degenerately doped direct band gap
semiconductor pn-junction whose band diagram is shown
in Fig.3
– Degenerate doping means that the Fermi level EFp in the p-side is
in the valence band (VB) and that EFn in the n-side is in the
conduction band (CB)
– All energy levels up to the Fermi level are occupied by electrons
• Without applied voltage, the Fermi level is continuous
across the diode, EFp= EFn.
– The depletion region is very narrow
– High potential energy barrier eVo (Vo is built-in voltage) that
prevents electrons in the n+-side diffusing into the p+-side
– Similar potential barrier also stop hole diffusion.
30. Forward bias
• When a voltage is applied, the separation
between EFp and EFn is due to electrical work
done by the applied voltage, DEF=eV.
• The applied voltage diminishes the built-in
potential barrier to almost zero
– Electrons flow into the Space Charge Layer (SCL)
and flow over to p+-side to constitute diode
current.
– Holes flow from p+-side to n+-side.
31. Active region:
population inversion
• From the energy band diagram with EFp – EFn =
eV >Eg as shown in Fig.3,
– there are more electrons in the CB at energies
near Ec than electrons in the VB near Ev.
– In other words, there is a population inversion
between energies near Ec and those near Ev
around the junction.
• This population inversion region is a layer
along the junction
– It is called the inversion layer or the active region
32. Stimulated emission & optical gain
• An incoming photon with an energy of (Ec – Ev) cannot
excite an electron from Ev to Ec as there are almost none
near Ev
– However, it stimulate an electron to fall down from Ec to Ev
– The incoming photon stimulates direct recombination
• The region where there is population inversion and hence
more stimulated emission than absorption
– The active region has an optical gain
• The optical gain depends on
– The photon energy as apparent by the energy distributions of
electrons and holes in the CB and VB in the active layer.
34. Optical Gain for T=0K & T>0K
• At T 0K, the states between Ec and EFn are filled with
electrons and those between EFp and Ev are empty.
– Photon with energy (Eg < hu < EFn – EFp) cause stimulated
emission
– whereas those photon with energy (EFn–EFp< hu) become
absorbed
• As T > 0K, the Fermi-Dirac function spreads the energy
distribution of electrons in the CB to above EFn and holes
below EFp in the VB
– The result is a reduction in optical gain as shown in Fig.4
– The optical gain depends on EFn–EFp (which depends on the
applied voltage and hence on the diode current)
35. Injection Pumping
• It is apparent that population inversion between
energies near Ec and those near Ev is achieved
– by the injection of carriers across the junction under a
sufficiently large forward bias.
• The pumping mechanism is therefore the forward
diode current
• The pumping energy is supplied by the external
battery
• This type of pumping is called injection pumping
36. Optical Cavity
• Optical cavity is also needed to implement a laser
oscillator
– to build up the intensity of stimulated emissions by
means of an optical resonator
– This would provide a continuous coherent radiation as
output
• Fig.5 shows schematically the structure of a homojunction
laser diode
– pn-junction with direct bandgap material like GaAs
– The ends of the crystal are cleaved to be flat and optically
polished to provide reflection and hence form optical
cavity
38. Mode of cavity
• The photons are reflected from the cleaved
surface stimulate more photons of the same
frequency
– This process builds up the intensity of the
radiation in the cavity
– The wavelength of the radiation is determined by
the cavity length L because only multiple of the
half-wavelength can exists
m (l/2n) = L
where m is an integer, n is the refractive index of the
semiconductor and l is free space wavelength
39. Resonant frequency
m (l/2n) = L
where l c/u (u is laser frequency)
• Each radiation satisfying the above
relationship is essentially a resonant
frequency of the cavity
– that is a mode of the cavity
– The separation between possible modes (allowed
wavelength) of the cavity Dlm.
40. Output spectrum of laser diode
• The exact output spectrum from the laser
diode depends on
1. The nature of optical cavity
2. The optical gain vs wavelength characteristic
• dependant on the energy distribution of electrons in
the CB and holes in the VB around the junction
41. Diode current
• Two critical diode current
1. Transparency current Itrans:
• provides just sufficient injection to lead to stimulated
emission just balancing absorption
• Above Itrans, there is optical gain in the medium but
output is not yet a continuous wave coherent radiation
2. Threshold current Ith:
• the optical gain in the medium has overcome the
photon losses from the cavity
• Lasing radiation is only obtained above Ith
42. Threshold current
• Fig.6 shows the output light intensity as a
function of diode current
– Above Ith, the light intensity becomes coherent
radiation consisting of cavity wavelength (or
mode) and increases steeply with current
– The number of modes in the output spectrum and
their relative strengths depends on the diode
current
44. Threshold current of homojunction
• The main problem of the homojunction laser
diode is that the threshold current density Jth is
too high for practical uses
– For GaAs at room temperature, Jth the order of 500
Amm-2
– GaAs laser can only operates continuously at very
low temperature.
• The reduction of Ith to a practical value requires
– Improvement in the rate of stimulated emission
– Improving the efficiency of the optical cavity
45. Reduction of the threshold current
1. Carrier confinement
– Confine the injected electrons and holes to a narrow
region around the junction
– Less current is needed to establish the necessary
concentration of carriers for population inversion
2. Photon confinement
– Build a dielectric waveguide around the optical gain region
to increase the photon concentration and the probability
of stimulated emission
– Can reduce the loss of photons traveling off the cavity axis