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DNA Metabolism in Plants
• DNA Replication
• DNA Repair
• Recombination
EUKARYOTIC DNA REPLICATION
• Origin of replication
• ARS Sites
• Helicase
• Topoisomerase
• DNA polymerase α delta epsilon
• Leading strand
• Lagging strand – Okazaki Fragments
• Telomerase
• The energetics of DNA Replication
PROTEIN SYNTHESIS
• Transcription
• Translation
TRANSCRIPTION
• Nucleus
• Template strand
• Promoter
• Enhancer
• Silencers
• Mediators
• RNA Polymerase I, II, III
• Molecular mechanism of
RNA Modification:-
- Capping
- Tailing
- Splicing.
TRANSLATION
• Cytoplasm
• Ribosomes – P site & A site
• AUG Start codon
• Translators of genetic code – t-RNA
• Translocation
• Termination codon
• Release factors
Alternative splicing in plants
 Regulates gene expression to over come stress in plants.
 Multiple protein isoforms are produced.
 RNA Editing in Plants.
 Post-Transcriptional RNA processing
 Chloroplast, Mitochondria
 Restoration of Codons phylogenetically conserved amino acid
 Jumping genes, Hopping genes, Barbara McClintock “Mobile controlling
elements” Nobel prize 1983 Transposons – Maize plant – Kernel colour – Ac/Ds
Transposons.
Plant Genome – Arabidopsis thaliana
THANK YOU

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Chapter 2 dna metabolism

  • 1.
  • 2. DNA Metabolism in Plants • DNA Replication • DNA Repair • Recombination
  • 3. EUKARYOTIC DNA REPLICATION • Origin of replication • ARS Sites • Helicase • Topoisomerase • DNA polymerase α delta epsilon • Leading strand • Lagging strand – Okazaki Fragments • Telomerase • The energetics of DNA Replication
  • 4.
  • 5.
  • 6.
  • 7. PROTEIN SYNTHESIS • Transcription • Translation TRANSCRIPTION • Nucleus • Template strand • Promoter • Enhancer • Silencers • Mediators • RNA Polymerase I, II, III • Molecular mechanism of RNA Modification:- - Capping - Tailing - Splicing.
  • 8. TRANSLATION • Cytoplasm • Ribosomes – P site & A site • AUG Start codon • Translators of genetic code – t-RNA • Translocation • Termination codon • Release factors
  • 9. Alternative splicing in plants  Regulates gene expression to over come stress in plants.  Multiple protein isoforms are produced.  RNA Editing in Plants.  Post-Transcriptional RNA processing  Chloroplast, Mitochondria  Restoration of Codons phylogenetically conserved amino acid  Jumping genes, Hopping genes, Barbara McClintock “Mobile controlling elements” Nobel prize 1983 Transposons – Maize plant – Kernel colour – Ac/Ds Transposons.
  • 10. Plant Genome – Arabidopsis thaliana