Hematopoietic stem cells in the bone marrow can differentiate into various blood cell types, including red blood cells, neutrophils, eosinophils, basophils, mast cells, monocytes/macrophages, dendritic cells, B lymphocytes, T lymphocytes, and natural killer cells. These cells play important roles in immunity, such as phagocytosis of pathogens by neutrophils and macrophages, antibody production by B cells, and cytotoxic killing by natural killer and T cells.
This is a brief presentation on the topic Cell Mediated Immunity describing how our immune system gets activated in response to any xenobiotic which is different from the mechanism followed by humoral immunity.
Immunology - Innate and Acquired ImmunityShigina E S
Title: Innate and Acquired Immunity: Understanding the Two Branches of Our Immune System
Introduction:
The human immune system is a complex network of cells, tissues, and organs that protects us from invading pathogens and foreign substances. In this presentation, we will explore the two branches of the immune system: innate and acquired immunity. We will discuss the key features of each branch, their mechanisms of action, and how they work together to keep us healthy.
Section 1: Innate Immunity
- Innate immunity is the first line of defense against pathogens and foreign substances.
- We will discuss the key features of innate immunity, including physical barriers, such as skin and mucous membranes, and the cellular and molecular components of innate immunity, such as phagocytes and cytokines.
- We will also explore some of the ways in which innate immunity can be activated and how it responds to different types of pathogens.
Section 2: Acquired Immunity
- Acquired immunity, also known as adaptive immunity, is a more specialized and targeted response to specific pathogens or foreign substances.
- We will discuss the key features of acquired immunity, including the role of B and T lymphocytes, antibodies, and memory cells.
- We will also explore some of the ways in which acquired immunity can be activated, including through vaccination, and how it responds to specific antigens.
Section 3: Interaction between Innate and Acquired Immunity
- Innate and acquired immunity work together in a coordinated manner to provide effective protection against pathogens and foreign substances.
- We will discuss how innate immunity can initiate an immune response and activate acquired immunity, and how acquired immunity can enhance the effectiveness of innate immunity.
- We will also explore some examples of how these two branches of the immune system work together in different types of infections.
Conclusion:
Understanding the different branches of our immune system is essential for developing effective strategies to prevent and treat infectious diseases. Innate and acquired immunity work together to provide a coordinated and dynamic defense against pathogens and foreign substances. By exploring the mechanisms and interactions between these two branches of the immune system, we can gain a deeper appreciation for the complexity and power of our immune system.
The organism possesses powerful mechanism to avoid immune auto aggression, The acquired ability of the immune system to avoid responsiveness to self antigens is defined as ‘ tolerance’ It is obtained by the cooperative efforts of central and peripheral mechanisms, which allow a rapid and efficient removal of pathogens ( Virus and Bacteria ) in the absence of self-recognition, It is a dysfunction of the immune system. The immune system protects you from disease and infection. Sometimes, though, the immune system can produce autoantibodies that attack healthy cells, tissues, and organs. This can lead to autoimmune disease.Autoimmune diseases can affect any part of the body
Immune tolerance, or immunological tolerance, or immunotolerance, is a state of unresponsiveness of the immune system to substances or tissue that have the capacity to elicit an immune response in a given organism. Immune tolerance is important for normal physiology. Central tolerance is the main way the immune system learns to discriminate self from non-self. Peripheral tolerance is key to preventing over-reactivity of the immune system to various environmental entities (allergens, gut microbes, etc.).
Antigen antibody interactions play important role in immunological assays which help in detection of disease.Such interaction are of various types e.g.Precipitation,Flocculation, Agglutination, Complement fixation, ELISA,RIA, Immunoflourescence,Immunoprecipitation.
This is a brief presentation on the topic Cell Mediated Immunity describing how our immune system gets activated in response to any xenobiotic which is different from the mechanism followed by humoral immunity.
Immunology - Innate and Acquired ImmunityShigina E S
Title: Innate and Acquired Immunity: Understanding the Two Branches of Our Immune System
Introduction:
The human immune system is a complex network of cells, tissues, and organs that protects us from invading pathogens and foreign substances. In this presentation, we will explore the two branches of the immune system: innate and acquired immunity. We will discuss the key features of each branch, their mechanisms of action, and how they work together to keep us healthy.
Section 1: Innate Immunity
- Innate immunity is the first line of defense against pathogens and foreign substances.
- We will discuss the key features of innate immunity, including physical barriers, such as skin and mucous membranes, and the cellular and molecular components of innate immunity, such as phagocytes and cytokines.
- We will also explore some of the ways in which innate immunity can be activated and how it responds to different types of pathogens.
Section 2: Acquired Immunity
- Acquired immunity, also known as adaptive immunity, is a more specialized and targeted response to specific pathogens or foreign substances.
- We will discuss the key features of acquired immunity, including the role of B and T lymphocytes, antibodies, and memory cells.
- We will also explore some of the ways in which acquired immunity can be activated, including through vaccination, and how it responds to specific antigens.
Section 3: Interaction between Innate and Acquired Immunity
- Innate and acquired immunity work together in a coordinated manner to provide effective protection against pathogens and foreign substances.
- We will discuss how innate immunity can initiate an immune response and activate acquired immunity, and how acquired immunity can enhance the effectiveness of innate immunity.
- We will also explore some examples of how these two branches of the immune system work together in different types of infections.
Conclusion:
Understanding the different branches of our immune system is essential for developing effective strategies to prevent and treat infectious diseases. Innate and acquired immunity work together to provide a coordinated and dynamic defense against pathogens and foreign substances. By exploring the mechanisms and interactions between these two branches of the immune system, we can gain a deeper appreciation for the complexity and power of our immune system.
The organism possesses powerful mechanism to avoid immune auto aggression, The acquired ability of the immune system to avoid responsiveness to self antigens is defined as ‘ tolerance’ It is obtained by the cooperative efforts of central and peripheral mechanisms, which allow a rapid and efficient removal of pathogens ( Virus and Bacteria ) in the absence of self-recognition, It is a dysfunction of the immune system. The immune system protects you from disease and infection. Sometimes, though, the immune system can produce autoantibodies that attack healthy cells, tissues, and organs. This can lead to autoimmune disease.Autoimmune diseases can affect any part of the body
Immune tolerance, or immunological tolerance, or immunotolerance, is a state of unresponsiveness of the immune system to substances or tissue that have the capacity to elicit an immune response in a given organism. Immune tolerance is important for normal physiology. Central tolerance is the main way the immune system learns to discriminate self from non-self. Peripheral tolerance is key to preventing over-reactivity of the immune system to various environmental entities (allergens, gut microbes, etc.).
Antigen antibody interactions play important role in immunological assays which help in detection of disease.Such interaction are of various types e.g.Precipitation,Flocculation, Agglutination, Complement fixation, ELISA,RIA, Immunoflourescence,Immunoprecipitation.
This presentation gives you the detailed description of various cells & organs of immune systems that participates (particularly, in combination), make communication between themselves to regulate the whole immune system very precisely.
The cells of the immune system arise from a pluripotent Hematopoietic Stem Cells (HSCs) through a process known as haematopoiesis.
Hematopoiesis involves the production, development, differentiation, and maturation of the blood cells (erythrocytes, megakaryocytes and leukocytes) from HSCs.
Differentiation of the HSC will occur along one of two pathways, giving rise to either a common myeloid progenitor or a common lymphoid progenitor cells in the presence of specific cytokines or soluble mediates (growth factor).
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
1. CELLS OF IMMUNE SYSTEM
Nidhi Saxena
Department of
Microbiology
2. INTRODUCTION
All blood cells arise from a type of cell called the hematopoietic stem cell
(HSC).
Stem cells are cells that can differentiate into other cell types; they are self-
renewing—they maintain their population level by cell division.
Hematopoiesis is the formation and development of red and white blood
cells.
In human, it starts in the embryonic yolk sac during the first weeks of
development.
In the third month of gestation, hematopoietic stem cells migrate to the fetal
liver and then to the spleen.
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 2
3. After birth, HSCs move to the bone marrow and differentiate to form
blood cells.
Hematopoietic stem cell is multipotent, or pluripotent, able to self-
renewal, can differentiate into erythrocytes, granulocytes, monocytes,
mast cells, lymphocytes, and megakaryocytes.
These stem cells are few (approx one HSC per 5 x 104 cells) in the bone
marrow.
INTRODUCTION
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4. Hematopoiesis is regulated at the genetic level
Factor Dependent lineage
GATA-1 Erythroid
GATA-2 Erythroid, myeloid, lymphoid lineages development
PU.1 Erythroid (maturational stages), myeloid (later stages),
lymphoid
Bmi-1 Self renewal capacity of HSC
Ikaros Lymphoid development.
Oct-2 B lymphoid (differentiation of B cells into plasma
cells)
Notch1 Regulates the choice between T and B lymphocyte
lineages
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6. CELLS OF IMMUNE SYSTEM
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7. Neutrophils are circulate 7-10 h in peripheral blood then migrate into the
tissues, where they live only a few days.
These cells generally are the first to arrive at a site of inflammation.
Some chemotactic factors (complement components, cytokines etc.)
promote accumulation of neutrophils at inflammatory site.
Phagocytosis by neutrophils is done by lytic enzymes and bactericidal
substances contained within primary and secondary granules.
Neutrophils express higher levels of defensins than macrophages.
NEUTROPHIL
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8. EOSINOPHILS
Eosinophils are motile phagocytic cells that can migrate from
the blood into the tissue spaces.
The secreted contents of eosinophilic granules may damage the
parasite membrane.
They play role in the defense against multicellular parasitic
organisms e.g. worms.
Eosinophils secrete cytokines that regulate B and T lymphocytes
and influencing the adaptive immune response.
Eosinophils are also contributors to asthma and allergy symptoms.
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 8
9. BASOPHIL
Basophils are nonphagocytic granulocytes that contain large granules
filled with basophilic proteins.
Basophils response against parasites, and cause allergy symptoms.
Basophils secrete cytokines that modulate the adaptive immune
response.
Basophils are function by releasing pharmacologically active
substances from their cytoplasmic granules. These substances play a
major role in certain allergic responses.
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 9
10. MAST CELLS
Mast cells are found in a various tissues, Example skin, connective
tissues of various organs, and mucosal epithelial tissue of the
respiratory, genitourinary, and digestive tracts.
Mast cells activated by microbial products binding to Toll Like
Receptors (TLRs).
Mast cell granules contain vasoactive amines such as histamine cause
vasodilation and increased capillary permeability and proteolytic
enzymes that can kill bacteria or inactivate microbial toxins.
Mast cells also synthesize and secrete lipid mediators and cytokines,
which stimulate inflammation.
Mast cells, with blood basophils causes the development of allergies.
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 10
12. It consists monocytes circulating in the blood and macrophages in the
tissues.
Promonocytes, leave the bone marrow and enter the blood, where they
differentiate into mature monocytes.
Monocytes circulate in the bloodstream for about 8 h, during which they
enlarge; then migrate into the tissues and differentiate into tissue specific
macrophages such as Intestinal macrophages found in the gut, Alveolar
macrophages found in the lung, Histiocytes found in connective tissues,
Kupffer cells found in the liver, Mesangial cells found in the kidney,
Microglial cells found in the brain and Osteoclasts found in bone.
MONONUCLEAR PHAGOCYTES
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 12
13. Differentiation of monocyte
into macrophage:
• Cell enlarges 5 to 10 folds
• Intracellular organelles
increase in number and
complexity
• Increased phagocytic
ability
• Produces higher levels of
hydrolytic enzymes
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 13
14. Phagocytosis
Macrophages are capable of ingesting
and digesting exogenous antigens
(whole microorganisms and insoluble
particles) and endogenous matter
(injured or dead host cells, cellular
debris, and activated clotting factors).
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 14
15. Dendritic cells (DC), first cells of the immune system to be discovered, acquired
its name because it is covered with long membrane extensions that resemble the
dendrites of nerve cells.
Immature dendritic cells capture antigens and then migrate to lymph nodes
where they present the antigen to T cells.
They take cargo in 3 ways: phagocytosis, internalize by receptor mediated
endocytosis or imbibe by pinocytosis.
Langerhans DCs (epidermis), Interstitial DCs (interstitial spaces), Monocyte-
derived DCs (arise from monocytes).
They all display class I and II MHC, CD80, CD86 and CD40 which required to
interacts with T cells.
DENDRITIC CELLS
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17. Follicular Dendritic Cells
• Do not arise in bone marrow.
• Do not express MHC Class II molecule and hence do not function as
antigen presenting cell.
• Present in lymph follicles (lymph nodes).
• High level of membrane receptors for antibody.
• Interaction of B cells with follicular dendritic cells is an important step
in the maturation and diversification of B cells.
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 17
18. B lymphocytes
They mature within the bone marrow and expresses antigen-binding
receptor (1.5 x 105 molecules of antibody).
When a naive B cell first encounters the antigen that matches its
membrane bound antibody, causes the cell to divide rapidly; its progeny
differentiate into memory B cells and effector B cells.
Memory B cells have a longer life span than naive cells, and they
express the same membrane-bound antibody as their parent B cell.
Plasma cells produce the antibody in secretary form (2000 molecules of
antibody per second) and live for only a few days.
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 18
20. T lymphocytes
Arise in the bone marrow and migrate to the thymus gland to mature
express a T-cell receptor on its membrane.
T-cell receptors can recognize antigen that is bound to major
histocompatibility complex (MHC) molecules.
Naive T cell encounters antigen with a MHC molecule, proliferates and
differentiates into T helper (display CD4) and T cytotoxic (display
CD8) cells.
The ratio of TH to TC cells is approximately 2:1 in normal human.
TH cells secrete various cytokines, which play a central role in the
activation of B cells, Tc cells, macrophages, and other cells.
4-Feb-21 NIDHI SAXENA, DEPARTMENT OF MICROBIOLOGY 20
22. Natural Killer Cells
Large, granular lymphocytes, show cytotoxic activity against a wide
range of tumor cells and against cells infected with some viruses.
NK cells recognize target such as tumor cells and cells infected by
certain viruses display antigens bound by antitumor or antiviral
antibodies.
NK cells express CD16, a membrane receptor for the carboxyl-
terminal end of the IgG molecule, can attach to these antibodies and
subsequently destroy the targeted cells, process known as antibody-
dependent cell mediated cytotoxicity (ADCC).
NKT cell have T cell receptors, Interact with CD1 instead of MHC class
I or II molecules.
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25. REFERENCES
1. Kuby, J., Goldsby, R. A, Kindt T. J., Osborne B. A. (2013). Immunology 7th
edition, W.H. Freeman and Company, New York.
2. Lyolyard, P. M., Whelan, A., Fanger. M. (2011) Instant Notes in Immunology.
3rd edition. Garland Science Taylor and Francis Group, Newyork
3. A. K. Abbas, A. H. H.Lichtman, S. Pillai. (2017).Molecular and Cellular
Immunity. 9th edition. Elsevier
4. C. A. Janeway, P. Travers, M. Walport, M. J. Shlomchick. (2005). Immunology –
the immune system in health and Diseases. 6th edition. Garland Science
Taylor and Francis Group, Newyork
5. K. Murphy, P. Travers, M. Walport. (2008). Janeway’s Immunology. 7th edition.
Garland Science Taylor and Francis Group, Newyork
6. J. M.Cruse, R. E. Lewis. (2009). Illustrated Dictionary of Immunology. 3rd
edition. CRC Press Taylor and Francis Group, New York.
7. Google
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