The document discusses electro discharge machining (EDM), a machining method used for hard, electrically conductive metals. EDM works by using electrical sparks to erode unwanted material away from a workpiece. This allows machining of metals that other techniques cannot cut. The document covers the history of EDM's development, how the process works using sparks and a dielectric fluid, and different EDM techniques like wire EDM and spark EDM as well as their applications and advantages/disadvantages.
EDM is a recent machining technique used to cut metals which can’t be cut with traditional methods. EDM works with materials of electrical conductivity.
EDM is a recent machining technique used to cut metals which can’t be cut with traditional methods. EDM works with materials of electrical conductivity.
Electric Discharge Machine ( E.D.M) - A Non Conventional Machining Process Gururam Prasad
Electric Discharge Machining is shortly called as EDM. In EDM the metal is removed due to erosion caused by rapidly occurring discharge between the tool and workpiece. The mechanism involved in Material removal is melting and evaporation.
Manufacturing & benefit of Brass Electrical Componentsasianfasteners
There are many advantages of working brass electrical parts. A few of them are included here. Thus, brass electrical components are widely used in different industries.
Electric Discharge Machine ( E.D.M) - A Non Conventional Machining Process Gururam Prasad
Electric Discharge Machining is shortly called as EDM. In EDM the metal is removed due to erosion caused by rapidly occurring discharge between the tool and workpiece. The mechanism involved in Material removal is melting and evaporation.
Manufacturing & benefit of Brass Electrical Componentsasianfasteners
There are many advantages of working brass electrical parts. A few of them are included here. Thus, brass electrical components are widely used in different industries.
it is a presentation on electrical discharge machining which is new method for machining hard conductive materials which are difficult to machine with old technique of machining. it does not cause wear of tool.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
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Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
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3. EDM IS A MACHINING METHOD
TYPICALLY USED FOR HARD METALS
WHICH ARE ELECTRICALLY CONDUCTIVE.
IT MAKES POSSIBLE TO WORK WITH
METALS FOR WHICH TRADITIONAL
MACHINING TECHNIQUES ARE
INEFFECTIVE.
4. WHAT IS EDM ?
Electro Discharge Machining
Used on hard metals
Works with electrically
Conductive materials
5. HISTORY OF EDM
In1770s, discovered by Joseph Priestly.
Developed in the mid 1970s
In the mid 1980s, the EDM techniques
were Transferred to a machine tool
Today it is a viable technique that helped
shape
The metal working industry.
6.
7. ACCESSORIES
Surface plate
Sine plate
Slip gauge
Dial indicator
Magnetic ‘V’ block
Precision vise
Pin gauge set
Tooling ball set
Special holders
8. BASIC OF E D M
Workpiece
Electrodes
Sparks
Dielectric fluid
Deionized water
Dielectric oil
Cutting speed
9.
10. HOW EDM WORKS?
Uses very powerful sparks (~3x106 V/m)
Series of rapidly recurring
Charges
Electroerosion
Remove excess by fluid
Flow
11. SPARKS
The phenomena “Breakdown”
occurs
A path for the current through
dielectric
Locally 10-15.000 ºC Ionize the
fluid
Metal melts or vaporizes
12. WIRE EDM
Needs wire feed
o Pre hole is required
for machining for passing
the wire
o Wire dia + spark gap is the
kerf
13. Applications for wire edm
Gears, splines
Machining of carbide
Cone shape angle profiles
Prototype production
14. Advantages Disadvantages
handles delicate tasks
highly accurate
very small kerf for wire
EDM
produces complex
shape
deep, or 3-D shapes
o wire wear out
oslow cutting rates
o heat affected local
stress formation.
oBlind holes can not be
produce
16. Applications for spark
edm
Slots, keyways, square & hex drives ,Gears
Small or deep holes, especially in hardened
material
Removal of broken taps and drills
Hardened jobs can be tapped (cnc edm)
Machining of carbide
17. Advantages Disadvantages
handles delicate tasks
may cut or drill very
hard materials
highly accurate
produces complex
shape
deep, or 3-D shapes
o electrode wear
oslow cutting rates
o heat affected local
stress formation.