The Characterization of Carbohydrates

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Benedict's reagent is used to test for reducing sugars like glucose, lactose, and fructose. It contains copper sulfate that is reduced to copper oxide when heated with reducing sugars, causing a color change from blue to green, brick red, or brown. Benedict's reagent can also detect glucose in urine samples to test for diabetes. Barfoed's reagent is similar but uses acetic acid, allowing it to distinguish between mono- and disaccharides. Disaccharides do not react for up to 10 minutes while monosaccharides form a blue or green precipitate. Sucrose is hydrolyzed using acid or enzyme to form glucose and fructose in the inversion test.

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The characterization of Carbohydrates CHEM 5 - BSN

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Based on the reactivity with Tollen’s, Benedict’s or Fehling’s reagent, carbohydrates are classified as; Reducing sugars Carbohydrates that can reduce Tollen’s, Benedict’s or Fehling’s reagents are called reducing sugars (sugar with free aldehyde or ketone group). All monosaccharides and most of the disaccharides are reducing sugars. Some examples are Maltose and Lactose. Non-reducing sugars Carbohydrates that cannot reduce Tollen’s, Benedict’s or Fehling’s reagents are called non-reducing sugars. Sucrose is a non-reducing sugar.

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Strong acids and strong bases always react in the same format since the dissociate nearly 100% in water. Solutions of hydrochloric acid and sodium hydroxide are mixed the reaction occurs as follows: Weak acids dissociate only slightly in water, and therefore should be left combined and not written as its ions. When weak acids react with strong bases, the H+ from the weak acid is transferred to the OH- from the strong base to form water and a salt. The salt formed, however, will most likely be soluble, and should be written as its respective ions. Remember also, to cancel out any spectator ions.

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