3. DEFINITION
An infectious disease
Mainly of respiratory tract of birds
Characterized by
Acute miliary infection of the lungs in young birds
Chronic air sac infection in adults
Respiratory distress, central nervous dysfunction,
sleepiness, inappetance emaciation, conjunctivitis
and cloudy eyes can be seen
4. ETIOLOGY
Many species of genus Aspergillus are involved
Mainly Aspergillus fumigatus A. niger and A. flavus
Family Moniliaceae
Characteristics:
Commonly occur in decaying vegetative matter, soil & feed
grains
Grow readily on most common laboratory media
Both major organisms lack a sexual stage.
7. TRANSMISSION
Moldy food or litter: Birds become infected
from inhaling spores
Contaminated incubators & hatchers
Egg shell contamination
The disease is not contagious and does not
spread from one bird to another.
8. PREDISPOSING FACTORS
High humidity & temperature favour growth of
fungus
Young age
Debility
Bad/poor management
9. Epidemiology
Aspergillus fumigatus / Aspergillus flavus, Aspergillus niger…
Overgrowth
Specific virulence factors ?
• Species
• Avian strains
• Individual susceptibility ?
• Age
• Sex
• Stress
• Other micro-organisms
• Toxicosis
• Therapeutics
captive environment
handling
migration…
mycotoxins ?
corticosteroids
turkeys, quails,
raptors, penguins,
parrots, waterfowl
10. CLINICAL SIGNS
Serous exudation from nasal & ocular
mucosa
Dyspnea, gasping & accelerated breathing
Somnolence, inappetence, emaciation
Dysphagia if oesophageal mucosa involved
Torticollis, lack of equilibrium, tremors, ataxia
Other respiratory diseases increase the
severity of acute aspergillosis
11. POST MORTEM LESIONS
Small white caseous nodules (approx. 1 mm
diameter) scattered throughout lung tissue
Caseous plaques on thickened air sacs that become
larger & numerous in advanced cases
Visible greenish gray mold growth may also be
observed on air sacs due to sporulation of organism.
Yellowish-green or whitish, caseous (cheesy)
nodules and/or green, fur-like down in mouth,
palate, trachea, syrinx, viscera, brain and eyes
28. DIAGNOSIS
Necropsy findings
Isolation & identification of organism
Sample sites: Caseous nodule in lung or air sac
Isolation of culture in 48 hours on Sabouraud dextrose
agar is diagnostic
Direct microscopy:
Small portion of nodule is teased in 20% KOH on a slide
& is covered with cover slip. (hyphae, mycelia,
conidophores)
Stain colony with lactophenol cotton blue to see
conidophores
Serologic tests are of limited use
Monitoring of hatchery, feed, litter & premises to find out
source of infection
32. DIFFERENTIAL DIAGNOSIS
Pullorum disease: Lesions of brooder
pneumonia are confined to respiratory
system while pullorum disease lesions occur
also in abdominal organs
CRD, ND & IB: Gasping & breathing is
rapid in brooder pneumonia but no
respiratory sounds.
MD & LL: In Aspergillosis nodules in the
lungs and fungal fur-like down in the air sacs
are diagnostic, which is not seen in MD & LL
33. IN CASE OF OUTBREAK
It is usually an infection of individual birds
rather than a flock problem
Sacrifice the affected birds
Spray pens with 1% CuSo4 solution
1: 12000 aqueous solution of CuSO4 in
drinking water.
34. CONTROL
Hatchery sanitation:
Regular fumigation of eggs
An aerosol of thiabenidazole or Clinafarm® pellets can be
placed in the hatchery to kill fungus
Formalin fumigation is an effective method to kill Aspergillus in
the environment
Feed & litter should be kept dry.
Access to moldy litter & feed should be avoided.
Daily cleaning & disinfection of feed and water utensils.
Proper ventilation
Overcrowding should be avoided.
Chlorine, and/or copper sulphate can be added to the water to
help reduce the spread of the organism
36. Thrush / candidiasis/ crop mycosis/
moniliasis
Also called moniliasis / crop mycosis
Candida albicans
Disease of upper GIT tract.
Characterized by whitish thickened areas of the
crop and proventriculus, erosions in the gizzard.
Crop is thickened by a soft ,yellow white irregular
false (pseudo) membrane (curd like appearance )
Mouth & esophagus show ulcer like patches.
Nystatin / Gentian violet drops