The document discusses the Bradford assay method for quantifying protein concentration. It begins by explaining the importance of accurately determining protein concentration when purifying and analyzing proteins. It then describes the Bradford assay, noting that it is a rapid, reliable method resistant to interference. The document outlines the principle and procedure of the Bradford assay. It explains that a calibration curve is generated by measuring the absorbance of protein standards to allow quantification of unknown protein concentrations.

1. BRADFORD ASSAY
BY- SAKET RANJAN
M.SC. PLANT BIOTECHNOLOGY
R.G.S.C (BHU)
EXAM ROLL NO.:-20430PLB025
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2. INDEX
 Protein quantification
 Bradford assay
 Bradford standard assay
 Principle of Bradford assay
 Procedure of bradford assay
 Measurement of protein concentration
 Calibration curve
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3. Protein quantification
 Protein quantification is an integral part of any
investigation related to protein isolation,
purification, characterization, and analysis.
 Accurate determination of protein concentration is
fundamental to all quantitative measurements of
biochemical Interactions.
 The protein quantitation assay should beRapid
,reliable and resistant to potentially interfering
substances.
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4. Protein quantification
 The time when accurate protein estimation is
needed :-
1. When a fractionation step is critically
dependent on protein concentration.
2. When it is necessary to know whether a
particular step has Really Removed much
unwanted protein.
3. To access the specific activity and follow the
progress of purification.
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5. Protein quantification
To the importance of protein concentration during
analysis and purification, several methods are
presented
 While choosing a method potential interfering
substances, availability of instrumentation and
desired sensitivity are considered.
 These methods are:-
1. Nano orange quantitation
2. Bicinchoninic acid(BCA)
3. Bradford method
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6. What is Bradford assay ?
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7. Bradford assay
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8. Continued.....
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9. Bradford standard assay
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10. Continued....
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11. Principle of Bradford assay
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Continued......

13. Procedure
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14. Continued.....
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15. Measurement of protein concentration
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16. Calibration curve
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17. Advantages of Bradford assay
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18. Disadvantages of Bradford assay
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