- BEF, also known as three-day sickness, is an acute viral disease affecting cattle and buffalos. It is transmitted by mosquitoes and biting flies.
- The disease causes economic losses due to reduced milk yield, loss of condition, and infertility. Clinical signs include fever, lameness, stiffness, and difficulty rising.
- Diagnosis is based on clinical signs during outbreaks and confirmed with serology or PCR testing. Control relies on vaccination, vector control, and farm hygiene measures.
etiology, local names, definition, transmission, source of infection, epidemiology, pathogenesis, clinical signs, diagnosis, differential diagnosis, treatment prevention and control
etiology, local names, definition, transmission, source of infection, epidemiology, pathogenesis, clinical signs, diagnosis, differential diagnosis, treatment prevention and control
Peste des Petits Ruminants (PPR) in India Epidemiology and ControlBhoj Raj Singh
PPR is endemic in India in sheep & goats. Mainly young stocks are more affected. Disease occurs throughout the year but more common in October & March. Though vaccination is the only method for control & eradication, even the institutes those developed the effective vaccine in India to control the disease fear to use it because many a time outbreaks ensue on vaccination. The other important reason for persistence of disease is undeclared Policy of suppressed reporting of PPR outbreaks.
Peste des Petits Ruminants (PPR) in India Epidemiology and ControlBhoj Raj Singh
PPR is endemic in India in sheep & goats. Mainly young stocks are more affected. Disease occurs throughout the year but more common in October & March. Though vaccination is the only method for control & eradication, even the institutes those developed the effective vaccine in India to control the disease fear to use it because many a time outbreaks ensue on vaccination. The other important reason for persistence of disease is undeclared Policy of suppressed reporting of PPR outbreaks.
The preliquisite for high economic returns is raising a healthy pig herd.
Unfortunately, today’s pig farmer does it as a by the way; keeps the pig under very unhygienic environment, a fertile ground for diseases .
Economic losses due to diseases arise as mortality and reduced growth rate.
Farmers Livestock School in Goat Production is a series of seminar and workshop conducted in Balanga, Bataan. It was conducted by the City Veterinary Office of City Government of Balanga. The activity includes a series of topics about goat breeding, goat nutrition, goat business, dairy goat and meat goat production, housing and facilities in goat production and other aspect in goat production business.
Dr. Paul Thomas - Porcine Epidemic Diarrhea Virus (PEDv)John Blue
Porcine Epidemic Diarrhea Virus (PEDv) - Dr. Paul Thomas, AMVC, LLC, from the 2015 World Pork Expo, June 3 - 5, 2015, Des Moines, IA, USA.
More presentations at http://www.swinecast.com/2015-world-pork-expo
Dr. Pete Lasley - The Dreaded Ps of Pork Production - PRRS and PEDVJohn Blue
The Dreaded Ps of Pork Production - PRRS and PEDV - Dr. Pete Lasley, Murphy-Brown of Missouri, LLC, from the 2014 Missouri Pork Expo , February 11 - 12, 2014, Columbia, MO, USA.
More presentations at http://www.swinecast.com/2014-missouri-pork-expo
Dr. Mike Eisenmenger - Driving Pig Performance in a Changing World of Antibio...John Blue
Driving Pig Performance in a Changing World of Antibiotic Usage, Boehringer Ingelheim Media Dinner - Dr. Mike Eisenmenger, Swine Vet Center, P.A., from the 2015 Allen D. Leman Swine Conference, September 19-22, 2015, St. Paul, Minnesota, USA.
More presentations at http://www.swinecast.com/2015-leman-swine-conference-material
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
1. BOVINE EPHEMERAL FEVER:
What you need to know and What you
need to do?
Dr. Muhammad Avais, DVM, PhD
Associate Professor
Department of Veterinary Medicine
University of Veterinary & Animal Sciences, Lahore
2. Synonyms of BEF
• Three-day sickness or three-day fever
• Bovine influenza
• Bovine epizootic fever
• Dengue of cattle
• Three-day stiffsickness
Vernacular name
• Vil
(Akakpo 2015)
3. What is BEF?
• Acute febrile viral disease
• Noncontagious arthropod borne
• Affects cattle and buffalo
• widespread in tropical and semitropical areas
• cause severe economic loss
• affected animals are only sick for a few days
• alternative name - Three Day Sickness
(Momtaz et al. 2012; oie 2016)
4. Why BEF is important?
• largely economic in nature
• does not cause significant mortality (1-2%)
• high case fatality rate in recent outbreaks
• main impact is on productivity
• decreased milk yield (at least 50%)
• rise in somatic cell count
• loss of condition
• reproductive losses (abortion, infertility)
• Recovery is prolonged
• Culling
• Trade restrictions
(oie 2016)
5. Who causes BEF?
• Bovine ephemeral fever virus
• member of genus
Ephemerovirus
• family Rhabdoviridae
• exist as a single serotype
worldwide
• VN tests on isolates from
Australia, China, Japan, Kenya,
Nigeria and South Africa have
demonstrated strong antigenic
cross-reactions
(Walker and Klement, 2015; Constables et al. 2017)
6. • vaccines developed in several countries using BEFV
strains isolated more than 40 years ago remain effective
against currently circulating strains
• vaccines developed against a strain of the virus from one
region are effective against viruses currently circulating
in other regions of the world
• homologous neutralisation titres are typically higher
than heterologous titres
Walker and Klement, 2015
Who causes BEF?
7. Temporal and phylogenetic relationships between bovine ephemeral fever
outbreaks of Asian countries
(Lee, 2019)
8. Species affected with BEF
• Cattle and yaks (both mem. of genus Bos)
• Water buffaloes (Bubalus bubalis)
• Antibodies found in sheep, goat and wild
animals
• All age groups susceptible
• Calves <3-6m not affected
• Bulls & High producing cows at greater risk
• no evidence of humans infections
(oie, 2016)
9. Geographic distribution
• Tropical and subtropical
areas of:
– Africa
– Asia
– Middle East
– Australia
• BEFV is not found in:
– Europe
– North or South America
– New Zealand
(Walker and Klement, 2015)
Countries in which BEF is known to occur
or from which the disease has been
reported historically
10. Morbidity and Mortality
• localized or seasonal epizootics
• spread influenced by prevailing winds
• mostly seen in summer and early fall
• outbreaks associated with high rainfall
• morbidity variable (80% or 1-10%)
• varies with age, condition and immunity
• mortality rate is 1-2% in most
outbreaks
• but can be 20-30% in cattle
(oie, 2016)
11. How BEFV is transmitted?
• Arthropods / hematophagus insects
• BEFV isolated from mosquitoes and
Culicoides
• Mosquitoes primary biological vectors
• Culicoides significant vectors in Africa
(Keynya)
• Wind-borne carriage of infected vector
• Intravenous inoculation of small
amounts of blood
(oie, 2016)
12. How BEFV is transmitted?
• No transmission between animals
• Not transmitted by
– Close contact
– body secretions
– aerosol droplets
• Not transmitted in semen
• Rapidly inactivated in meat
• no evidence of animal becomes carrier
(oie, 2016)
13. How BEF Develops?
• virus inoculation by vector (mosquitoes)
• Vascular System (Virus Multiplication)
• Pyrogen and neutrophils influx in bulk (Fever)
• Vasculitis and thrombosis
• Increased endothelial permeability
• Synovial cavities (Sero-fibrinous inflammation)
• Generalized Inflammation
14.
15. Clinical Signs
• IP; most cases b/t 2-4d, Max. 10-11d
• signs vary in individual animals
• classic course begins with fever
(biphasic to polyphasic)
• Sharp fall in milk production
• depressed, stiff or reluctant to move
• increased heart rate, tachypnea
• serous or mucoid nasal discharges
• Profuse salivation, muscle twitching
• waves of shivering or lacrimation
16. Clinical Signs
• Shifting lameness, stiffness and joint
pain
• joints may or may not be swollen
• submandibular or periorbital edema
• Dyspnea, pulmonary emphysema and
rales in severe cases
• Recombancy
• Bloat, ruminal stasis, or lose
swallowing reflex
• Temporary lose reflexes/unable to rise
• Exacerbate by environmental stress
or forced exercise
• Video
17. Causes of death
– Aspiration pneumonia
& Pneumonia from
secondary infection
– Pulmonary
emphysema
– Muscle damaged and
inflammation from long
period lying down
18. Complications
• temporary or permanent paralysis
• gait impairment
• aspiration pneumonia
• Pulmonary and subcut Emphysema
• mastitis
• Temporary infertility in bulls (up to 6
months)
• abortions in pregnant cows
• In recovered animals, milk production
is generally decreased for the rest of
the lactation
• but usually returns to normal after
subsequent pregnancies
• Cows that become ill late in lactation
may not return to production
19. Postmortem lesions
• Small amounts of fibrin-rich fluid in:
– pleural, peritoneal, pericardial cavities
– joint capsules
• Serofibrinous:
– Polysynovitis, polyarthritis
– polytendinitis and cellulitis
• Emphysematous lungs
• Enlarged edematous lymph nodes
• Focal necrosis in major muscle groups
20. How to diagnose BEF?
Clinical
• usually diagnosed clinically during
outbreaks in endemic areas
• This disease should be suspected in
cattle herds that develop severe but
transient symptoms including fever,
lameness, temporary paralysis or
recumbency
• Difficult to diagnose when a single animal
is affected
21. How to diagnose BEF?
Hematology/Biochemistry
• absolute rise in leukocyte in acute
cases
• A rapid fall in circulating lymphocytes
• A return to normal levels after 3-4 days
• Low total serum calcium, Zn and Fe
• Plasma fibrinogen elevated
• CK elevated
22. How to diagnose BEF?
Laboratory tests
• Most cases of BEF are confirmed
by serology
• A rising titer should be
demonstrated with ELISA -- Paired
samples
• CFT can also be used
• RT-PCR
24. CONTROL MEASURES
(I) Vaccination
• All animals on the farms above 6-months of age may be
primed using live attenuated BEF vaccine at the start of
summer
• Make sure vaccine is not expired and the storage and
handling requirements are strictly adhered
• Booster dose of vaccine 4 weeks post-priming
• Annual booster of vaccine
25. (II) Vector Control
• BEF virus spreads through biting flies, mosquitoes
• Fly repellents such as Cypermethrin mixed with liquid
paraffin
• Apply on Pouron method from head to tail. Repeat after
every 7 days
• Spray of Seguvon / Neguvon @ 0.1% on breeding places
of vectors (floors, walls, manure, slurry, etc)
• Remove stagnant water from the dairy farm
• Proper disposal of manure / slurry
CONTROL MEASURES
26. Control of Ticks
• Weekly spray of cypermethrin on farm
• Application of lime on dry floors
• Filling of crevices (Breeding sites) on floors / walls, etc
• Elimination of wooden structures on floors
CONTROL MEASURES
27. Monitoring and control for blood parasites
• Animals are often having subclinical form of blood
parasites that may be the prime cause of vaccination
failure
• Monitor the herd for hemoparasites through random
selected samples
• If a herd or particular group of cows is positive for blood
parasites, treat as per nature of the disease.
CONTROL MEASURES
28. III. Movement restriction
• Movement of cattle from infected herd or area should be
strictly banned during the outbreak/epidemic
• Avoid new purchases and introduction of new animals
into the herd. (if necessary, put all new purchases to
strict quarantine)
CONTROL MEASURES
29. IV. Herd Monitoring
• Feed and water consumption and production should be
carefully monitored at all times
• Typical sounds and behaviors of individual animals
should be monitored
• Temperature of randomly selected animals should be
monitored
CONTROL MEASURES
30. V. Biosecurity
• Strictly adhere to all farm biosecurity measures
• Do not allow visitors during the outbreak
• Restrict the entry of service personals and vendors
•
• Disinfect farm and farm premises using disinfectants
(Virkon or 2% pyodine or 5% Bleach)
CONTROL MEASURES
31. TREATMENT STRATEGY
(I) Therapeutics
• Early detection of the clinically sick cases through
continuous monitoring
• Administration of NSAIDs
• Immune boosters such as Vitamin E and Selenium
• Calcium therapy (calcium borogluconate / Milfone-C, I/V)
• Antibiotics to control secondary bacterial infection like
Ceftiofur sodium
• Cold water therapy to lower body temperature
• Follow single needle use policy when treating or
vaccinating the cows.
32. (II) Nutrition and Environmental
Management
• Increase level of calcium,
selenium, vitamin C and vitamin E
•
• Reduce stock Density
• Avoid overcrowding
• Disease normally followed rainy
season due to hot humid climate
TREATMENT STRATEGY
33. (III) Isolation of sick cows
• Keep the infected/diseases
animals in isolation and
don’t allow them to mix with
healthy ones
TREATMENT STRATEGY
34. (IV) Management of recumbent cows
• Nursing care- Animals that have gone down should
be provided with adequate shelter, water and food,
as cattle left exposed in hot weather are much more
likely to die
• BEF can impair the swallowing reflex, so affected
animals should not be drenched or force fed
• Recumbent cows are rolled over several times a day
to help avoid loss of circulation to the underside
limbs, which will result in permanent muscle
damage.
TREATMENT STRATEGY
35. • Ensure that animal is in sternal position: Cattle that go
into lateral recombancy (lying flat) can bloat and die or
aspirate rumen fluid which can cause pneumonia
• Place sand bags, hay bales to prop up animals that
cannot remain sternal without assistance
• Calcium injections- if given early in the course of the
illness, can be very effective in helping an animal regain
its footing.
TREATMENT STRATEGY
36. Disinfection
• BEFV is not thought to be transmitted directly between
animals, and does not persist for long periods in the
environment
• If disinfection is needed, this virus is reported to be very
susceptible to disinfectants, including sodium
hypochlorite, and lipid solvents
TREATMENT STRATEGY
37. Summary
• BEF also know as three-day-sickness
• Noncontagious arthropod borne viral disease caused by
BEFV of genus ephemerovirus
• Affects cattle and water buffalo
• Economically important due to reduced milk yield, loss of
condition, infertility, trade restriction
• Transmitted by mosquitoes and biting flies
• Prevalence in tropical and subtropical areas
• High morbidity during outbreaks and high case fatality
rate
38. • BEFV causes vasculitis and increases vascular
permeability
• Polyserositis and polyarthritis
• Clinical signs are fever, shifting lameness, stiffness, joint
pain and recombancy
• Pulmonary emphysema is the complication
• Diagnosis by clinical picture, serology and PCR
• Control through vaccination, vector control and farm
hygiene
• Treat with NSAIDs, immune-boosters and calcium
Summary
Editor's Notes
Bovine ephemeral fever (BEF), also known as three-day sickness or three-day fever [1], is an arthropod-borne viral disease that mainly strikes cattle and water buffalo.. Historically, BEF has also been named bovine influenza, bovine, epizootic fever [2], styfsieket, or dengue of cattle [3], This disease was first recorded in the late 19th century
Bovine Ephemeral Fever (BEF) is an acute febrile viral non-contagious arthropod borne disease of cattle and buffalo. Widespread in tropical and semitropical areas of the Eastern Hemisphere that can cause severe economic loss. Typically, affected animals are only sick for a few days, hence the alternative name - Three Day Sickness
Bovine ephemeral fever is an economically important arboviral disease that affects cattle and water buffalo, and is widespread in tropical and semitropical areas of the Eastern Hemisphere. In most cases, the main impact is on productivity. While the illness is usually brief, it can result in decreased milk yield, loss of condition and reproductive losses, and recovery can be prolonged in some animals. Mortality is typically low (1-2%); however, significantly higher case fatality rates were reported in some recent outbreaks.
The importance of BEF is largely economic in nature, however, it does not cause significant mortality (1-2%). In most cases, the main impact is on productivity. In recent outbreaks high case fatality rate of 25-30% was reposted in exotic dairy herds. The main impact of the disease is on productivity, with decreases milk production. Milk production usually drops by at least 50% in sick cows. In dairy herds it is the highest producing animals that are usually the most severely affected. Yield should return to normal after about three weeks. Bulls and fat cows tend to show more severe signs than other cattle. Such animals lose condition rapidly and are slow to regain their body weight. A proportion of bulls will suffer temporary infertility lasting from three to six months because of the high fever. Permanent infertility is uncommon but can occur. Recovery is prolonged and severe chronic cases may lead to increased culling.
Bovine ephemeral fever is caused by bovine ephemeral fever virus (BEFV), a member of the genus Ephemerovirus in the family Rhabdoviridae.
BEFV is considered to exist as a single serotype worldwide. Virus neutralisation tests conducted using isolates from Australia, China, Japan, Kenya, Nigeria and South Africa have demonstrated strong antigenic cross-reactions [25–29].
Structure and morphology of BEFV. A Structural organization of the 14.9 kb BEFV genome shown as arranged in negative sense. Structural protein genes (N, P, M, G and L) are shown in black and the various accessory genes are coloured. B Transmission electron micrograph showing BEFV virions and defective-interfering (DI) particles. Scale bar 100 nm. Reproduced from Walker [7] with permission from Springer-Verlag. C Structural model of a monomeric subunit of the BEFV G protein derived by homology modelling using the pre-fusion form of the VSV G protein as a template. The model illustrates the three major neutralization sites (G1, G2 and G3a/b) and amino acid residues shown to be under positive selection in Australia [18]. Adapted with permission from the American Society for Microbiology.
There is also anecdotal evidence that vaccines developed in several countries using BEFV strains isolated more than 40 years ago remain effective against currently circulating strains and that vaccines developed against a strain of the virus from one region are effective against viruses currently circulating in other regions of the world. Nevertheless, homologous neutralisation titres are typically higher than heterologous titres amongst viruses isolated at different times or from different geographic regions [1, 27, 28].
Only cattle and yaks (both members of the genus Bos) and water buffalo (Bubalus bubalis) are known to be affected by BEFV. Camels (Camelus dromedarius) can be seropositive, and a disease resembling ephemeral fever has been reported in this species, but its cause is still unknown. Antibodies to BEFV have also been found in asymptomatic sheep, goats and pigs, and in many wild animals including African buffalo (Syncerus caffer), hartebeest (Alcelaphalus buselaphus), waterbuck (Kobus ellipsiprymnus), wildebeest (Connochaetes taurinus), kudu (Tragelaphus strepsiceros), giraffe (Giraffa camelopardalis), elephant (Loxodonta africana), hippopotamus (Hippopotamus amphibius), warthog (Phacochoerus aethiopicus) and various species of deer and antelope. Seroprevalence can be high in some African wildlife, and some species might act as reservoir hosts. However, cross-reactivity with similar viruses complicates the interpretation of serological tests. Experimental infections have been established in sheep, but there is currently no evidence that this species plays any role in the epidemiology of bovine ephemeral fever in nature.
Zoonotic potential
There is no evidence that humans can be infected by BEFV.
Geographic Distribution
It occurs over a vast expanse of the globe from the southern tip of Africa to the Nile River Delta, across the Middle East through South and South-East Asia, into northern and eastern Australia, and throughout most of China, extending into Taiwan, the Korean Peninsula and southern Japan (Figure 1). BEFV does not occur in the islands of the Pacific, Europe (other than in the western regions of Turkey) or in the Americas where, for quarantine purposes, it is considered as an important exotic pathogen.
Morbidity and Mortality
Bovine ephemeral fever can occur as localized outbreaks or in seasonal epizootics. During epizootics, the pattern of spread seems to be influenced by the prevailing winds. Most cases are seen in the summer and early fall, and outbreaks are often associated with high rainfall.
The morbidity rate is highly variable, and can be as high as 80% or as low as 1-10%. Morbidity varies with the age and condition of the animal, as well as any immunity it may have. The mortality rate is 1-2% in most outbreaks, but it can be as high as 30% in cattle.
Transmission
BEFV appears to be transmitted by arthropods, but the identity of the vector or vectors is not entirely clear. This virus has been isolated from various genera of mosquitoes, and from a number of Culicoides species (biting midges). Laboratory observations, together with epidemiological evidence from some locations, currently suggest that mosquitoes are the primary biological vectors. However, there are some indications that Culicoides might be significant vectors in parts of Africa (e.g., Kenya). Windborne transmission of infected vectors has been suspected in some outbreaks.
There is no evidence that bovine ephemeral fever can be transmitted directly between animals in nature; BEFV is not spread by close contact, body secretions, or aerosol droplets. However, animals can be infected in the laboratory by intravenous inoculation of small amounts of blood. This virus does not seem to be transmitted in semen and it is rapidly inactivated in meat. There is no evidence that animals become carriers.
Incubation Period
Based on natural and experimental infections, the incubation period is thought to be 2-4 days in most cases, with a maximum of 10-11 days.
Clinical Signs
Clinical signs vary in individual animals, but the classic course begins with a fever, which is often biphasic to polyphasic. The temperature peaks typically occur 12 to 18 hours apart. In lactating cows, milk production often drops dramatically during the first fever spike. There are often few other clinical signs at this time, although some animals may be depressed, stiff or reluctant to move. However, the illness soon becomes more obvious; most animals become inappetent and depressed, with an increased heart rate, tachypnea, and serous or mucoid discharges from the nose. Profuse salivation, muscle twitching, waves of shivering or lacrimation may also be seen.
Some animals develop submandibular or periorbital edema, or patchy edema on the head
Shifting lameness, stiffness and joint pain are common; the joints may or may not be swollen. The lameness can be severe enough to mimic a fracture or dislocation. Dyspnea, pulmonary emphysema and rales may be found in severe cases. Many animals, particularly cows in good condition and bulls, become recumbent for periods that range from 8 hours to several days or more. Most animals lie in sternal recumbency, but in severe cases, animals may become laterally recumbent. Some animals temporarily lose their reflexes and are unable to rise. Recumbent animals may be bloated, have ruminal stasis, or lose their swallowing reflex. These clinical signs can be exacerbated by severe environmental stress or forced exercise.
Most animals begin to improve a day or two after the initial signs, and recover completely within another 1-2 days. Lactating cows, bulls and animals in good condition are usually affected more severely, and may take up to a week to recover. Generally, animals lose condition rapidly during the illness, and regain their weight only slowly. Complications are uncommon in most outbreaks, but can include temporary or (rarely) permanent paralysis, as well as gait impairment, aspiration pneumonia, emphysema, mastitis, and the subcutaneous accumulation of air along the back. Many of these complications may be the result of trauma or complications of recumbency. Temporary infertility (up to 6 months) can develop in bulls, and abortions sometimes occur in cows. Permanent infertility is rare. In recovered animals, milk production is generally decreased for the rest of the lactation, but usually returns to normal after subsequent pregnancies. Cows that become ill late in lactation may not return to production. Death is uncommon in most outbreaks, but may occur during either the febrile or the convalescent stage. During recent outbreaks in China, some severely ill cattle died with signs of dyspnea, 6-12 hours after they first became ill. Secondary complications such as pneumonia or trauma are thought to contribute significantly to the death rate. Subclinical infections are also seen.
Water buffalo have similar signs. The disease is usually thought to be milder in this species; however, some severe outbreaks have been reported in the field. Experimentally infected sheep remained asymptomatic.
Post Mortem Lesions
The most obvious lesion is a small amount of fibrin-rich fluid in the pleural, peritoneal and pericardial cavities, resulting from polyserositis of the pleural, pericardial and peritoneal surfaces. Edema, lobular congestion and atelectasis may be apparent in the lungs, and emphysematous lesions are sometime detected in the lungs, mediastinum and subcutaneous connective tissue. Serofibrinous polysynovitis (with variable amounts of yellow to brown, typically gelatinous fluid), polyarthritis, polytendinitis, and cellulitis are common. Other lesions can include lymphadenitis, edema and petechial hemorrhages in the lymph nodes, and areas of focal necrosis in the major muscle groups.
Diagnostic Tests
Most cases of bovine ephemeral fever are confirmed by serology. A rising titer should be demonstrated, but single serum samples may be suggestive in areas where this disease does not normally occur. Anamnestic (rather than primary) responses to BEFV can occur during the first exposure, if the animal was previously exposed to another ephemerovirus. Virus neutralization or enzyme-linked immunosorbent assays (ELISAs) are the most commonly used serological tests. Some of these tests, including certain ELISAs, can distinguish BEFV from other members of the Ephemerovirus genus. Complement fixation was mainly used in the past, and identifies the antibodies only as Ephemerovirus-specific.
Reverse transcription polymerase chain reaction (RT-PCR) assays are used regularly for diagnosis in some countries. These assays may be able to detect viral RNA in blood during the (typically short) febrile period, and from tissue samples such as the lung at necropsy. A real-time loop-mediated isothermal amplification (RT-LAMP) assay has been published. Virus isolation may occasionally be successful, especially during the first 24-48 hours. The virus is often recovered initially in Aedes albopictus (mosquito) cell lines, and propagated in BHK-21 or Vero cells. The identity of the virus can be confirmed by RT-PCR, virus neutralization and some ELISAs. Immunofluorescence has also been used, but may be able to identify the virus only as an Ephemerovirus.
While animal inoculation is generally discouraged if there are alternatives, BEFV can be recovered by intracerebral inoculation into unweaned mice.
Differential Diagnosis:
(I) Therapeutics
Early detection of the clinically sick cases through continuous monitoring (Cardinal signs such as drop in milk production, fever, dull, depressed, off feed, increased respiration, lameness and staggering gait to recombancy)
Administration of non-steroidal anti-inflammatory drugs such as Phenylbutazone / ketoprofen / flunixin meglumine at recommended doses and route
Immune boosters such as Vitamin E and Selenium preparations (E-sel / Selevit)
Calcium therapy if needed (calcium borogluconate / Milfone-C, I/V at recommended dose)
Antibiotics to control secondary bacterial infection like Ceftiofur sodium at recommended doses
Cold water therapy on body to lower body temperature
Follow single needle use policy when treating or vaccinating the cows.