Hydrocarbon pollution Remediation by Enhanced
Natural Attenuation method was adopted to remediate the
hydrocarbon impacted site in Ogoniland Rivers State, Nigeria .
The research lasted for 6 months. Samples were collected at
monthly intervals . samples were collected intermittently
between Feb 2019 to July 2019 . Mineral salt medium containing
crude oil was used as a sole source of carbon and energy for the
isolation of hydrocarbonoclastic bacteria. Samples were
collected from the four (4) local government that made up
Ogoniland and they includes Khana(k), Gokana (G),Tai (T),
Eleme (E) and transported immediately to the laboratory for
analysis. The microbial and physicochemical properties of the
soil samples varied with the different local government areas.
Seven bacteria genera were isolated from the samples from the
four locations, viz, Pseudomonas, Lactobacter, Micrococcus,
Arthrobacter, Bacillus, Brevibacterium and Mycobacterium
were isolated and identified. the seven isolate were indigenous in
the study area. Nutrient were added to identified plots of
hydrocarbon pollution polluted site within the four local
government and they were able degrade hydrocarbon within a
short of period of time. Reassessment of physicochemical
parameter impacted site was used to judge the bioremediation
potentials of microorganism
Microbiological and Physico chemical Examination of Crude Oil Contaminated So...ijtsrd
Crude oil is a complex mixture of aliphatic and aromatic hydrocarbons that causes a variety of risks when released into agricultural and aquatic environment. This oil can alter population dynamics and disrupt tropic integrations and the structure of natural communities within ecosystems during spills. Microbiological and Physico chemical properties of crude oil and pristine soil samples were assessed using standard procedures. Most frequently occurring microbial isolates obtained were identified using molecular typing. Bacteria isolated from the contaminated soil were Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Pseudomonas fluorescens, Pseudomonas aeruginosa, Alcaligenes faecalis and Klebsiella pneumoniae. Fungi isolated were Penicillium citrinnum, Aspergillus fumigatus, Aspergillus flavus and Rhizopus stolonifer. The most frequently occurring novel isolates were S. maltophilia, P. sordelli, B. thuringensis, F. solani, C. deightonni, P. citrinnum, and C. bertholletiae. Percentage organic carbon content of the crude oil contaminated soil was 21.50 while the nitrogen content was 0.90 with a pH value of 4.69. This research showed that crude oil contamination of agricultural soil distorts its physico chemical parameters which has consequent toxicity on the soil, thus possibly hampering its fitness for use in crop production. Anagboso, M. O. | Orji, M. U. | Ikele, M. O. "Microbiological and Physico-chemical Examination of Crude Oil Contaminated Soil from Awka Anambra State Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46442.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/46442/microbiological-and-physicochemical-examination-of-crude-oil-contaminated-soil-from-awka-anambra-state-nigeria/anagboso-m-o
RECYCLING OF SOLID WASTES INTO ORGANIC FERTILIZERS USING LOW COST TREATMENT: ...ijiert bestjournal
Increasing in the human population,urbanization an d change in life style has increased the waste load and there by pollution loads on the urban envi ronment to unmanageable and alarming proportions. The existing waste dumping sites are f ull beyond capacity and under unsanitary conditions leading to pollution of water sources an d spreading communicable diseases,foul smell and odors,release of toxic metabolites,unae sthetic ambiance and eye sore etc. Vermcomposting is the better option to tackle with this problem. It is the process of conversion of organic wastes by earthworm to valuable humus li ke material which is used as a natural soil conditioner.Vermicompositing is environment friendl y and cost effective technique for solid waste management. It serves two main purposes for t he welfare of humans as it helps in the degradation of solid waste and the cast produced du ring this process is used as a natural fertilizer. This technique is much better than chem ical fertilizer because it is not associated with any kind of risk. Earthworms are potentially import ant that are capable of transforming garbage into gold.
Biochar impact on physiological and biochemical attributes of spinachGJESM Publication
Disastrous effect of nickel on spinach was discussed by number of authors but the effect of amendments like biochar with nickel on Spinacea oleraceaL. is not still discussed by any author of the world because biochar was used as soil amendments which play a vital role in reducing mobilization and uptake of nickel by spinach plants. As nickel contaminated plants are very harmful for the consumption by living organisms. Nickel can be gathered in agronomic soils by anthropogenic actions such as Ni-Cd batteries. In this study, the growth, physiological, photosynthetic and biochemical responses of Spinacia oleracea grown in Ni-spiked soil (0, 25, 50 and 100 mg Ni/Kg soil) at three levels of cotton-sticks-derived biochar “CSB” (0, 3 and 5 %) were evaluated. The results exposed significant decrease in growth, photosynthetic, physiological, and biochemical traits of S. oleracea when grown in Ni-polluted soil. However, this decrease was less pronounced in CSB amended soil. A steady rise in the MDA (0.66 µg/g to 2.08 µg g-1), ascorbic acid (1.24 mg/g to 1.57 mg/g)and sugar concentrations (1.73 mg/g to 2.16 mg/g)was observed with increased concentration of Ni. The increasing percentages of CSB from 3 % to 5 % decreased Ni concentrations in root and shoot of experimental plant. Higher production of chlorophyll, amino acids and protein with CSB amendment looked like alleviation in Ni toxicity. Therefore, it is concluded that, Ni toxicity and availability to the plants can be reduced by CSB amendments.
Characterization, treatment and recovery of fish byproduct as a stable bio-fe...Skyfox Publishing Group
National fish production reached in 2013 an amount of 1,245,912 tons. Now the pelagic fish industry generates a
significant amount of waste up to 60%. The physicochemical analyses of these fish by-products showed a wealth of organic material
including proteins (87.4% dry weight) and minerals (9% dry weight). These components and others are capitalizing to be used for
agricultural or agri-food. Biotransformation of biotechnologically in fish products was performed in the laboratory. Fresh fish byproducts
were ground and mixed with a carbon-rich source of carbohydrates. The mixture was inoculated with selected fermentation
yeast. The fermentation of evolution was controlled by monitoring chemical parameters (pH, dry matter, ash, total nitrogen) and
microbiological (FMAT, Enterobacteriaceae: fecal and total Coliforms, yeasts). Then the product thus stabilized at a pH of 3.8 has
undergone a second treatment. A hydrolyzate rich in amino acids and trace elements was obtained with excellent hygienic quality after a
total elimination of pathogens. Its use will be as factors for fertilizer and soil amendment. We produced three biostimulants necessary for
plant growth: Rooting fertilizer, Plant elongation fertilizer and production fertilizer. The application tests on these biofertilizers in the
laboratory were performed and have allowed obtaining promising results.
Microbiological and Physico chemical Examination of Crude Oil Contaminated So...ijtsrd
Crude oil is a complex mixture of aliphatic and aromatic hydrocarbons that causes a variety of risks when released into agricultural and aquatic environment. This oil can alter population dynamics and disrupt tropic integrations and the structure of natural communities within ecosystems during spills. Microbiological and Physico chemical properties of crude oil and pristine soil samples were assessed using standard procedures. Most frequently occurring microbial isolates obtained were identified using molecular typing. Bacteria isolated from the contaminated soil were Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Pseudomonas fluorescens, Pseudomonas aeruginosa, Alcaligenes faecalis and Klebsiella pneumoniae. Fungi isolated were Penicillium citrinnum, Aspergillus fumigatus, Aspergillus flavus and Rhizopus stolonifer. The most frequently occurring novel isolates were S. maltophilia, P. sordelli, B. thuringensis, F. solani, C. deightonni, P. citrinnum, and C. bertholletiae. Percentage organic carbon content of the crude oil contaminated soil was 21.50 while the nitrogen content was 0.90 with a pH value of 4.69. This research showed that crude oil contamination of agricultural soil distorts its physico chemical parameters which has consequent toxicity on the soil, thus possibly hampering its fitness for use in crop production. Anagboso, M. O. | Orji, M. U. | Ikele, M. O. "Microbiological and Physico-chemical Examination of Crude Oil Contaminated Soil from Awka Anambra State Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46442.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/46442/microbiological-and-physicochemical-examination-of-crude-oil-contaminated-soil-from-awka-anambra-state-nigeria/anagboso-m-o
RECYCLING OF SOLID WASTES INTO ORGANIC FERTILIZERS USING LOW COST TREATMENT: ...ijiert bestjournal
Increasing in the human population,urbanization an d change in life style has increased the waste load and there by pollution loads on the urban envi ronment to unmanageable and alarming proportions. The existing waste dumping sites are f ull beyond capacity and under unsanitary conditions leading to pollution of water sources an d spreading communicable diseases,foul smell and odors,release of toxic metabolites,unae sthetic ambiance and eye sore etc. Vermcomposting is the better option to tackle with this problem. It is the process of conversion of organic wastes by earthworm to valuable humus li ke material which is used as a natural soil conditioner.Vermicompositing is environment friendl y and cost effective technique for solid waste management. It serves two main purposes for t he welfare of humans as it helps in the degradation of solid waste and the cast produced du ring this process is used as a natural fertilizer. This technique is much better than chem ical fertilizer because it is not associated with any kind of risk. Earthworms are potentially import ant that are capable of transforming garbage into gold.
Biochar impact on physiological and biochemical attributes of spinachGJESM Publication
Disastrous effect of nickel on spinach was discussed by number of authors but the effect of amendments like biochar with nickel on Spinacea oleraceaL. is not still discussed by any author of the world because biochar was used as soil amendments which play a vital role in reducing mobilization and uptake of nickel by spinach plants. As nickel contaminated plants are very harmful for the consumption by living organisms. Nickel can be gathered in agronomic soils by anthropogenic actions such as Ni-Cd batteries. In this study, the growth, physiological, photosynthetic and biochemical responses of Spinacia oleracea grown in Ni-spiked soil (0, 25, 50 and 100 mg Ni/Kg soil) at three levels of cotton-sticks-derived biochar “CSB” (0, 3 and 5 %) were evaluated. The results exposed significant decrease in growth, photosynthetic, physiological, and biochemical traits of S. oleracea when grown in Ni-polluted soil. However, this decrease was less pronounced in CSB amended soil. A steady rise in the MDA (0.66 µg/g to 2.08 µg g-1), ascorbic acid (1.24 mg/g to 1.57 mg/g)and sugar concentrations (1.73 mg/g to 2.16 mg/g)was observed with increased concentration of Ni. The increasing percentages of CSB from 3 % to 5 % decreased Ni concentrations in root and shoot of experimental plant. Higher production of chlorophyll, amino acids and protein with CSB amendment looked like alleviation in Ni toxicity. Therefore, it is concluded that, Ni toxicity and availability to the plants can be reduced by CSB amendments.
Characterization, treatment and recovery of fish byproduct as a stable bio-fe...Skyfox Publishing Group
National fish production reached in 2013 an amount of 1,245,912 tons. Now the pelagic fish industry generates a
significant amount of waste up to 60%. The physicochemical analyses of these fish by-products showed a wealth of organic material
including proteins (87.4% dry weight) and minerals (9% dry weight). These components and others are capitalizing to be used for
agricultural or agri-food. Biotransformation of biotechnologically in fish products was performed in the laboratory. Fresh fish byproducts
were ground and mixed with a carbon-rich source of carbohydrates. The mixture was inoculated with selected fermentation
yeast. The fermentation of evolution was controlled by monitoring chemical parameters (pH, dry matter, ash, total nitrogen) and
microbiological (FMAT, Enterobacteriaceae: fecal and total Coliforms, yeasts). Then the product thus stabilized at a pH of 3.8 has
undergone a second treatment. A hydrolyzate rich in amino acids and trace elements was obtained with excellent hygienic quality after a
total elimination of pathogens. Its use will be as factors for fertilizer and soil amendment. We produced three biostimulants necessary for
plant growth: Rooting fertilizer, Plant elongation fertilizer and production fertilizer. The application tests on these biofertilizers in the
laboratory were performed and have allowed obtaining promising results.
Application nanotechnology in agricultural improvementSAGARDEEP SINHA
What is nanotechnology, how nanoparticles are formed, what are the areas of nanotechnology in agriculture, and how it is different than conventional agriculture, examples, and what are the cons?
In order to clean up soils contaminated with hydrocarbons, the bioremediation activity of Pseudomonas putida was studied. Pseudomonas putida is a bacterium that can withstand the harshest environmental conditions. It is able to metabolize a wide range of petroleum hydrocarbons which is used as a source of carbon and energy. Given the potential of this microorganism, an experiment wasconducted on this strain.
For the isolation of this microorganism, a sample ofsoil from the Vakinankaratra region in the urban commune of Antsirabe II, Madagascar was microbiologically analysed. The bacterial identification was based on a study of the morphological, physicochemical and sequential analysis of the 16S rDNA gene.
Mycological flora of Clarias gariepinus exposed to an oilfield wastewater in ...Innspub Net
The Mycological flora of Clarias gariepinus exposed to sub-lethal concentrations of an oilfield wastewater were investigated. The concentrations included 0% (control), 10, 20, 30, 40, 50 and 60% respectively. Physico-chemistry and mycoflora of wastewater and tissues of Clarias gariepinus were
determined using standard methods. Mean values obtained were; temperature 25.93±6.7oC, pH 7.73±0.31, turbidity 40.33±1.53 NTU, salinity 6584±137mg/l, conductivity 15200±1058.68μs/cm, total dissolved solids 8436.33±501.68 mg/l, total suspended solids 4.67±0.58mg/l, alkalinity
1296.33±2168mg/l, dissolved oxygen 1.83±0.38mg/l, biochemical oxygen demand 1.3±0.7mg/l and Total hydrocarbon 40.54±50mg/l. Temperature, DO, BOD and THC were below allowable FEPA limits while all other components were higher. Mean counts of total fungi and petroleum degraders in the oilfield wastewater were 4.7±0.46x106 sfu/ml and 59.7±25.7% respectively. Fungal counts in the
tissues of Clarias ranged from 0.20±0.00 x 104sfu/g to 3.00±0.00 x 104sfu/g (skin), 0.48±0.05 x 104sfu/g to 7.25±0.96 x 104sfu/g (gills), and 1.13±0.15 x 104sfu/g to 5.75±0.50 x 104sfu/g (intestine). The intestine had higher fungal counts, but the gills recorded the highest at 10%
concentration. Fungi isolated included; Aspergillus fumigatus (46.43%), Aspergillus niger (100%), Fusarium spp. (100%), Mucor spp. (24.99%), Penicillium spp. (57.14%), Rhizopus spp. (32.13%) and Saccharomyces spp. (34.3%). All except Saccharomyces spp were isolated from oilfield wastewater.
Aspergillus spp. Penicillium spp, Mucor and Rhizopus are considered normal flora, but can still cause infection which may result in the mortality of the fish and eventually economic loss to the aquarium fish industry. Proper treatment of oilfield wastewater prior to discharge into the recipient water body is
advocated to reduce ecotoxicological problems. Get more articles at: http://www.innspub.net/volume-3-number-1-july-2015-ijmm/
Nanotechnology and its application in postharvest technology by l. jeebit singhJeebit Singh
A presentation on basics of Nanotechnology and its application in Postharvest Technology. A credit seminar presentation as a part of fulfillment of my Master's Degree Programme during M.Sc. 1st year 2nd semister at PG Centre, Bangalore, University of Horticultural Sciences.
Studies on the impact of crude oil exploration on soil quality and crops grow...Premier Publishers
The present study evaluated the impact of crude oil exploration on soil quality and crops grown in Kpean Community in Khana Local Government Area of Rivers State. Soil samples were collected at a depth of 0-50cm3 using plastic auger while tuber crops and vegetables were harvested from the same spot as the soils. Findings from this study showed an increase in soil temperature, moisture, electrical conductivity and organic matter content of crude oil impacted soil compared to control (P<0.05).soil><0.05).><0.05)><0.05). The results of this study indicates that crude oil pollution of agricultural farmlands in Kpean Community is yet to be remedied even after 20 years following oil spillage and the soil still remain unsuitable for crop production. Hence, proper remediation of the studied area is paramount in order to reduce metal accumulation and subsequent exposure of the populace to metal poisoning via food chain.
Isolation and Screening for Citric Acid Production by Aspergillus Niger Using...ijtsrd
The numerous applications of citric acid have increased its demand. It is of great importance to produce citric acid using adequate monosaccharides and dissacharides as they have proven to be easily catabolyzed and decomposed by Fungi when compared to polysaccharides. The superiority of sucrose to other sugars is a result of strong extracellular mycelium bound invertase of A.niger which rapidly hydrolyses sucrose at low pH. This project was undertaken to isolate, screen and produce citric acid in a submerged fermentation by four different strains of Aspergillus niger using sucrose. Aspergillus niger was isolated and characterized from garden soil. Citric acid production from Aspergillus niger in a submerged basal medium was quantitatively determined using titrimetric method. Aspergillus niger strain HUS1 ASHUS1 , Aspergillus niger strain HUS7 ASHUS7 , Aspergillus niger strain HG49 ASHG49 and Aspergillus niger strain AN8 ASAN8 showed significant accumulation of citric acid from the submerged basal medium, of which ASHUS 1 significantly a 0.05 recorded the most yield from the production after 9 days of incubation. Mbah, O. G | Okpalla, J. O | Osuala, O. J | Okoye, P. O | Alagbu, P. O "Isolation and Screening for Citric Acid Production by Aspergillus Niger Using Sucrose as a Carbon Source" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46260.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/46260/isolation-and-screening-for-citric-acid-production-by-aspergillus-niger-using-sucrose-as-a-carbon-source/mbah-o-g
Use of stable and radio isotopes to understand the plant physiological processRAHUL GOPALE
Introduction
what is isotope ?
Types of Isotopes
Isotopic Labelling
ADVANTAGES AND DISADVANTAGES OF ISOTOPIC STUDY
APPLICATIONS OF ISOTOPES IN AGRICULTURE
Principle isotopes used in plant-soil studies
Case studies
FUTURE THRUSTS OF ISOTOPIC STUDY
CONCLUSIONS
REFERENCES
Hydrocarbon Biodegradation Potential of Cyanobacteria in Oil Polluted Soilijtsrd
Petroleum hydrocarbon is one of the most common environmental pollutants in Nigeria and other countries. Its debilitating effects on arable lands and water bodies have far reaching consequences to agriculture and aquatic life. Several bioremediation strategies have been developed over the years in a bid to manage this type of pollution. Cyanobacteria are a group of microorganisms possessing the potential to be harnessed as bioremediation alternatives. This study sought to conduct bioremediation of crude oil polluted oil usung cyanobacteria. Organisms were isolated from crude oil polluted soil and were identified using molecular typing. A 200 ml portion of broth cultures of organisms was used singly and in consortium in different set ups to bio remedaite the polluted soil samples. Gas chromatography was used to monitor the residual total petroleum hydrocarbon TPH in each set up for a period of thirty days. Microcystisholsatica, Chlorella kessleriand Anabaena cicadae were identified from polluted soil samples. Chlorella kessleri gave a 94.10 TPH loss, while Anabaena cicadae gave 91.10 and their co culture set up gave a 95 TPH loss and the control experiment had 2.10 TPH loss. Temperature of the reacting systems was mesophilic and the pH was at weak acid range. This study thus has shown that cyanoremediation of petroleum hydrocarbon pollution of soil is possible and efficient and should be adopted more often Agu, K. C. | Orji, M. U. | Ikele, M. O. | Uwanta, L. I. | Onyeneho, V. I "Hydrocarbon Biodegradation Potential of Cyanobacteria in Oil Polluted Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-7 , December 2022, URL: https://www.ijtsrd.com/papers/ijtsrd52397.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/52397/hydrocarbon-biodegradation-potential-of-cyanobacteria-in-oil-polluted-soil/agu-k-c
Application nanotechnology in agricultural improvementSAGARDEEP SINHA
What is nanotechnology, how nanoparticles are formed, what are the areas of nanotechnology in agriculture, and how it is different than conventional agriculture, examples, and what are the cons?
In order to clean up soils contaminated with hydrocarbons, the bioremediation activity of Pseudomonas putida was studied. Pseudomonas putida is a bacterium that can withstand the harshest environmental conditions. It is able to metabolize a wide range of petroleum hydrocarbons which is used as a source of carbon and energy. Given the potential of this microorganism, an experiment wasconducted on this strain.
For the isolation of this microorganism, a sample ofsoil from the Vakinankaratra region in the urban commune of Antsirabe II, Madagascar was microbiologically analysed. The bacterial identification was based on a study of the morphological, physicochemical and sequential analysis of the 16S rDNA gene.
Mycological flora of Clarias gariepinus exposed to an oilfield wastewater in ...Innspub Net
The Mycological flora of Clarias gariepinus exposed to sub-lethal concentrations of an oilfield wastewater were investigated. The concentrations included 0% (control), 10, 20, 30, 40, 50 and 60% respectively. Physico-chemistry and mycoflora of wastewater and tissues of Clarias gariepinus were
determined using standard methods. Mean values obtained were; temperature 25.93±6.7oC, pH 7.73±0.31, turbidity 40.33±1.53 NTU, salinity 6584±137mg/l, conductivity 15200±1058.68μs/cm, total dissolved solids 8436.33±501.68 mg/l, total suspended solids 4.67±0.58mg/l, alkalinity
1296.33±2168mg/l, dissolved oxygen 1.83±0.38mg/l, biochemical oxygen demand 1.3±0.7mg/l and Total hydrocarbon 40.54±50mg/l. Temperature, DO, BOD and THC were below allowable FEPA limits while all other components were higher. Mean counts of total fungi and petroleum degraders in the oilfield wastewater were 4.7±0.46x106 sfu/ml and 59.7±25.7% respectively. Fungal counts in the
tissues of Clarias ranged from 0.20±0.00 x 104sfu/g to 3.00±0.00 x 104sfu/g (skin), 0.48±0.05 x 104sfu/g to 7.25±0.96 x 104sfu/g (gills), and 1.13±0.15 x 104sfu/g to 5.75±0.50 x 104sfu/g (intestine). The intestine had higher fungal counts, but the gills recorded the highest at 10%
concentration. Fungi isolated included; Aspergillus fumigatus (46.43%), Aspergillus niger (100%), Fusarium spp. (100%), Mucor spp. (24.99%), Penicillium spp. (57.14%), Rhizopus spp. (32.13%) and Saccharomyces spp. (34.3%). All except Saccharomyces spp were isolated from oilfield wastewater.
Aspergillus spp. Penicillium spp, Mucor and Rhizopus are considered normal flora, but can still cause infection which may result in the mortality of the fish and eventually economic loss to the aquarium fish industry. Proper treatment of oilfield wastewater prior to discharge into the recipient water body is
advocated to reduce ecotoxicological problems. Get more articles at: http://www.innspub.net/volume-3-number-1-july-2015-ijmm/
Nanotechnology and its application in postharvest technology by l. jeebit singhJeebit Singh
A presentation on basics of Nanotechnology and its application in Postharvest Technology. A credit seminar presentation as a part of fulfillment of my Master's Degree Programme during M.Sc. 1st year 2nd semister at PG Centre, Bangalore, University of Horticultural Sciences.
Studies on the impact of crude oil exploration on soil quality and crops grow...Premier Publishers
The present study evaluated the impact of crude oil exploration on soil quality and crops grown in Kpean Community in Khana Local Government Area of Rivers State. Soil samples were collected at a depth of 0-50cm3 using plastic auger while tuber crops and vegetables were harvested from the same spot as the soils. Findings from this study showed an increase in soil temperature, moisture, electrical conductivity and organic matter content of crude oil impacted soil compared to control (P<0.05).soil><0.05).><0.05)><0.05). The results of this study indicates that crude oil pollution of agricultural farmlands in Kpean Community is yet to be remedied even after 20 years following oil spillage and the soil still remain unsuitable for crop production. Hence, proper remediation of the studied area is paramount in order to reduce metal accumulation and subsequent exposure of the populace to metal poisoning via food chain.
Isolation and Screening for Citric Acid Production by Aspergillus Niger Using...ijtsrd
The numerous applications of citric acid have increased its demand. It is of great importance to produce citric acid using adequate monosaccharides and dissacharides as they have proven to be easily catabolyzed and decomposed by Fungi when compared to polysaccharides. The superiority of sucrose to other sugars is a result of strong extracellular mycelium bound invertase of A.niger which rapidly hydrolyses sucrose at low pH. This project was undertaken to isolate, screen and produce citric acid in a submerged fermentation by four different strains of Aspergillus niger using sucrose. Aspergillus niger was isolated and characterized from garden soil. Citric acid production from Aspergillus niger in a submerged basal medium was quantitatively determined using titrimetric method. Aspergillus niger strain HUS1 ASHUS1 , Aspergillus niger strain HUS7 ASHUS7 , Aspergillus niger strain HG49 ASHG49 and Aspergillus niger strain AN8 ASAN8 showed significant accumulation of citric acid from the submerged basal medium, of which ASHUS 1 significantly a 0.05 recorded the most yield from the production after 9 days of incubation. Mbah, O. G | Okpalla, J. O | Osuala, O. J | Okoye, P. O | Alagbu, P. O "Isolation and Screening for Citric Acid Production by Aspergillus Niger Using Sucrose as a Carbon Source" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46260.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/46260/isolation-and-screening-for-citric-acid-production-by-aspergillus-niger-using-sucrose-as-a-carbon-source/mbah-o-g
Use of stable and radio isotopes to understand the plant physiological processRAHUL GOPALE
Introduction
what is isotope ?
Types of Isotopes
Isotopic Labelling
ADVANTAGES AND DISADVANTAGES OF ISOTOPIC STUDY
APPLICATIONS OF ISOTOPES IN AGRICULTURE
Principle isotopes used in plant-soil studies
Case studies
FUTURE THRUSTS OF ISOTOPIC STUDY
CONCLUSIONS
REFERENCES
Hydrocarbon Biodegradation Potential of Cyanobacteria in Oil Polluted Soilijtsrd
Petroleum hydrocarbon is one of the most common environmental pollutants in Nigeria and other countries. Its debilitating effects on arable lands and water bodies have far reaching consequences to agriculture and aquatic life. Several bioremediation strategies have been developed over the years in a bid to manage this type of pollution. Cyanobacteria are a group of microorganisms possessing the potential to be harnessed as bioremediation alternatives. This study sought to conduct bioremediation of crude oil polluted oil usung cyanobacteria. Organisms were isolated from crude oil polluted soil and were identified using molecular typing. A 200 ml portion of broth cultures of organisms was used singly and in consortium in different set ups to bio remedaite the polluted soil samples. Gas chromatography was used to monitor the residual total petroleum hydrocarbon TPH in each set up for a period of thirty days. Microcystisholsatica, Chlorella kessleriand Anabaena cicadae were identified from polluted soil samples. Chlorella kessleri gave a 94.10 TPH loss, while Anabaena cicadae gave 91.10 and their co culture set up gave a 95 TPH loss and the control experiment had 2.10 TPH loss. Temperature of the reacting systems was mesophilic and the pH was at weak acid range. This study thus has shown that cyanoremediation of petroleum hydrocarbon pollution of soil is possible and efficient and should be adopted more often Agu, K. C. | Orji, M. U. | Ikele, M. O. | Uwanta, L. I. | Onyeneho, V. I "Hydrocarbon Biodegradation Potential of Cyanobacteria in Oil Polluted Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-7 , December 2022, URL: https://www.ijtsrd.com/papers/ijtsrd52397.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/52397/hydrocarbon-biodegradation-potential-of-cyanobacteria-in-oil-polluted-soil/agu-k-c
Influence of Organic Wastes on Ecotoxicity of Petroleum Hydrocarbons in Conta...Premier Publishers
This study was aimed to evaluate the effect of organic wastes on the biodegradation of petroleum hydrocarbons in a contaminated soil. Three groups of soil samples (60 kg per group) were contaminated with four petroleum products (spent engine oil, petrol, diesel and kerosene), mixed together in a volumetric ratio of 1:1:1:1. The first group was contaminated with 5% weight/weight (w/w) of the mixed petroleum products; the second group was contaminated with 10% (w/w) of the mixed petroleum products; while the third group was contaminated with 15% (w/w) of the mixed petroleum products. In the bioremediation process, 3 kg of soil from each contaminated group (5%, 10% and 15% contamination) was filled into plastic containers, and amended with 0.5 kg cattle dungs and 0.5 kg rice husk, and coded CD + RH; while another 3 kg from each contaminated group was filled into plastic containers, and amended with 0.5 kg cattle dungs and 0.5 kg sawdust, and was coded CD + SD. The total hydrocarbon content (THC) and ecotoxicity of the soils samples were determined in accordance to standards procedures. The results revealed that the soil contaminated with 5% petroleum products generally had the highest biodegradation rate. In term of the ecotoxicity of the petroleum hydrocarbons, the cucumber planted in the amended soil samples had a lower radicle growth inhibition rate; when compared with the control soil samples. This study revealed that a combination of organic waste materials can be effectively utilized in the remediation of petroleum hydrocarbons in contaminated soils.
LABORATORY STUDIES ON THE BIOREMEDIATION OF SOIL CONTAMINATED BY DIESEL IAEME Publication
The most widely used energy and fuel resources are hydrocarbons such as crude oil and petroleum distillates. The accidental discharge of these petroleum products contribute in making hydrocarbons the most common environmental pollutants. Bioremediation helps to destroy or render harmless various contaminants using natural biological activity. The present study utilizes the potential of bioremediation to remediate soil contaminated with diesel. Eight bioreactors were used for the study, out of which four bioreactors were maintained at optimum environmental conditions and the remaining four were kept without any maintenance to serve as control bioreactors. Contaminated soil was prepared by mixing fresh soil and diesel so as to attain 10% TPH concentrations by weight of soil. Each bioreactor was filled with 3 kg of contaminated soil.
Evaluation of the Effectiveness of Fungi (Candida Tropicalis and Aspergillus ...IJEABJ
Used engine oil is a petroleum or synthetic oil that has been used and as a result of such use, is contaminated by physical and chemical pollutants. These pollutants are harmful to humans, animals and plants following exposure. Evaluation of the effectiveness of fungi in bioremediation of used engine oil (UEO) contaminated soil was investigated. Fungi were isolated from soil samples obtained from automobile workshops in Mgbuka-Nkpor, Nigeria. The isolates were screened for UEO biodegradation potentials in mineral salt broth. They were identified using the cultural and microscopic characteristics and confirmed using the 18SrRNA gene sequence. The effectiveness of the isolates in bioremediation of UEO contaminated soil was also investigated using bioaugmentation technique. A total of 8 fungal isolates were obtained from this study. Two that showed the highest extent of biodegradation of UEO in the screen flasks were identified and confirmed as Candida tropicalis and Aspergillus clavatus. At the end of the experimental period, oil contaminated soil inoculated with the mixed culture of the isolates (C. tropicalis and A. clavatus) showed the highest reduction in concentration of UEO (95.42%). Higher biodegradation rate and shorter half-life of total petroleum hydrocarbon (TPH) was observed in soil microcosm containing the isolates, when compared to the uninoculated control. Therefore fungi such as C. tropicalis and A. clavatus isolated from automobile workshops can facilitate the bioremediation of UEO contaminated soil.
Anaerobic Co-Digestion of Cassava Waste Water and Abdominal Cow Dung under Ch...IJCMESJOURNAL
Anaerobic co-digestion of cassava waste water (CWW) and abdominal cow dung (ACD)in different proportions was studied in five treatments under changing meteorological conditions. The five treatment cases T1: 100% CWW; T2: 100% ACD; T3: 90%CWW +10%ACD; T4:70%CWW+30%ACD; T5: 50%CWW+50%ACD were digested under anaerobic conditions in model batch, metallic bio digesters of same working volume (32.0 liters) for 30 days retention period. Results indicated that T2 system flamed on the 20th day, T5 on the 20th day while T1, T4 and T3 systems didn’t flame. T1 had cumulative gas yield of 12.7 liters; T2 had 28.85 liters; T3 had 12.5 liters,T4 had 11.1 liters while T5 had cumulative gas yield of 15.8 liters per 24kg mass of slurry. T2had 91.20% methane; while T5 produced 92.999% methane. Daily biogas yields were modeled as functions of meteorological parameters. Results indicated that many parameters showed good correlations with ambient temperature.
The International Journal of Engineering and Science (The IJES)theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
Phytotoxicity and Microbial Activities of Crude Oil Polluted Pristine Soil du...ijtsrd
The continuous drive to increase the production, distribution and utilization of petroleum and natural gas products to meet the energy demands of the population is usually associated with some environmental pollution problems especially on the lithosphere soil and the physicochemical remediation approaches such as evaporation, dissolution, dispersion with chemical dispersants emulsifiers, and photo oxidation all found to be eco harzardous and non cost ineffective. Microbiological and Physico chemical properties of pristine soil contaminated with crude oil were assessed using standard procedures. The influence of saw dust in the remediation of the polluted soil was evaluated. Phytotoxicity of the polluted soil was assessed using maize and beans seed germination test. Most frequently occurring microbial isolates obtained were identified using molecular typing. Bacteria isolated from the pristine soil were Bacillus subtilis, Bacillus cereus, Bacillus thuringiensis, Pseudomonas fluorescens, Pseudomonas aeruginosa, Alcaligenes faecalis and Klebsiella pneumoniae. Fungi isolated were Penicillium citrinnum, Aspergillus fumigatus, Aspergillus flavus and Rhizopus stolonifer. The most frequently occurring novel isolates were S. maltophilia, P. sordelli, B. thuringensis, F. solani, C. deightonni, P. citrinnum, and C. bertholletiae. Percentage organic carbon content of the crude oil polluted soil was 21.50 while the nitrogen content was 0.90 with a pH value of 4.69. The Percentage organic carbon of the pristine soil was 0.750 , percentage nitrogen content was 2.71 with a pH value of 5.69. This research showed that phytotoxicity test carried out on the crude oil polluted soil distorted its physico chemical parameters which has consequent toxicity on the soil, thus possibly hampering its fitness for use in crop production. Phytotoxicity test on the saw dust amended soil significantly supported plant growth with a high germination index 1G for both crops maize and beans therefore making the soil fit for use in crop production. Anagboso, M. O. | Orji, M. U. | Ikele, M. O. "Phytotoxicity and Microbial Activities of Crude-Oil Polluted Pristine Soil during Bioremediation with Saw Dust Amendment from Awka, Nigeria" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd47560.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/47560/phytotoxicity-and-microbial-activities-of-crudeoil-polluted-pristine-soil-during-bioremediation-with-saw-dust-amendment-from-awka-nigeria/anagboso-m-o
ABSTRACT This research paper presents the microbial and hydrobiological indicators and the physicochemical quality of water samples from a lentic ecosystem in Ibeno LGA, Nigeria, after sixteen (16) years of an aviation fuel spill. Using culture-dependent methodologies, the hydrocarbonoclastic bacterial and fungal counts (HBC & HFC) ranged from 3.4 x 104 to 1.2 x 105cfu/l and 4.7 x 103 to 1.8 x 104 cfu/l, respectively with the ratios of total heterotrophic bacterial counts to HBC and total fungal counts to HFC ranging from 8 to 12% and 15 to 22%, respectively. Predominant bacterial indicators included Pseudomonas aeruginosa, Bacillus subtilis, Micrococcus varians and Enterobacter aerogenes while predominant fungal indicators included Aspergillus niger, A. terreus, Candida sp, Saccharomyces sp, Phoma sp. and Botrytis sp. Predominant zooplanktons in the sampled area were rotatoria while the least were nematodes and followed the trend: Rotatoria > Copepoda > Cladocera > Nematoda. Water samples from the area showed evidence of oil sheen when disturbed; with pH values (6.2 to 7.8) tending generally towards neutral. Total petroleum hydrocarbon (TPH) from this aviation fuel-contaminated lentic system ranged from 81.5 mg/l to 505.2 mg/l. Dissolved oxygen (DO) were generally low with high BOD and COD of 46.3 mg/l and 321.1mg/l, respectively. Other physicochemical parameters were typical of lentic ecosystems in the Niger Delta region, Nigeria. The impact of this and many other spills are enormous. This confirms that it takes a long time for recovery once the environment is polluted.
Key-words: Aquatic pollution, Aviation fuel, Hydrobiological parameters, Hydrocarbonoclastic Microorganisms, Lentic ecosystem, Physicochemical characteristics
Microbiological and Physicochemical Assessment of Poultry Soil Samples in Aku...Agriculture Journal IJOEAR
Abstract— Human activities such as animal production in many parts of the world, still impact negatively on the environment and biodiversity. This study was carried out to assess the microbiological and physicochemical parameters of poultry soil samples. Soil samples were collected from different poultry in Akure metropolis and soil samples from Federal University of Technology, Akure (FUTA) environment as control. Microbiological and physicochemical analyses were carried out using standard methods. The mean total viable bacterial count of poultry soil ranged from 9.02±0.511×105cfu/g in sample site A to 11.2±0.021×105cfu/g in sample site B and there were significant difference (p<0.05) between the bacterial count of poultry soil and control, mean highest fungal load is 6.05±0.301× 103sfu/g. Bacteria isolated were Escherichia coli, Aeromonas hydrophila ,Bacilllus subtilis, Bacillus cereus, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Streptococcus faecium. Escherichia coli had the highest occurrence of 23.08% while Aeromonas hydrophila had the lowest occurrence of 5.13%. Fungi isolated were Aspergillus flavus, Aspergillus niger, Aspergillus fumigates and Saccharomyces species. Aspergillus niger 46.67% had the highest occurrence while Saccharomyces species had the lowest occurrence of 13.33%. Physiochemical analysis showed that poultry soil had mean pH, temperature and Organic Carbon of 7.92±0.34, 34±0.04oC and 14.88±0.56% respectively, Organic Matter is 5.50±0.61%, Nitrogen 1.27±0.03 mg/g, Phosphorus58.92±0.01 mg/g, Potassium55.48±0.23 mg/g, Sodium 41.77±0.91mg/g, Calcium 28.70±0.24mg/g, Magnesium 20.65±0.32 mg/g and Cation exchange capacity146.60±0.11%. The results obtained calls for proper discharge of poultry waste into the environment to prevent transmission of diseases by water borne pathogens.
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ABSTRACT- The development of human civilization throughout history has led to growing disruption of the natural
balance and the occurrence of different types of pollution. Environmental pollution with petroleum and petrochemical
products has been recognized as significant and serious problem. Diesel engine oil, which is one of the major products of
crude oil, constitutes a major source of pollution in our environment. Therefore diesel engine oil can enter into the
environment through wrecks of oil tankers carrying diesel oil, cleaning of diesel tanks by merchants, war ships carrying
diesel oil and motor mechanics. In present study the microorganisms utilising petrol and diesel oil as carbon source were
isolated and investigation of their characteristics towards the production of polyhydroxyalkanoates (PHA), which is now a
days well known as biodegradable polymer.
Key Words- Petrol and Diesel oil contamination, Bioremediation, Biodegradable bacterial polymer, Sudan
Black B staining, 16sr RNA sequencing
Spatial Distribution of Residual Petroleum Hydrocarbons in an Oil Spill Site ...Premier Publishers
The study was done to evaluate the residual total hydrocarbon content (THC) concentration in an oil spill site within Delta State, Nigeria. To achieve this study’s objective, soil samples were collected from two sites (contaminated site and control site) within Delta state. The soil samples were collected from the contaminated site (between Oleh and Idheze communities of Delta State) about nine months after the oil spill and the clean-up by the oil company. The THC concentration of all the soil samples collected were analyzed in accordance with standard ASTM recommended procedures. Results obtained from the laboratory tests showed that the THC concentrations were higher in the already cleaned up oil spill site, when compared with the results obtained from the control site. This indicates a significant residual THC concentration in the already cleanup oil spill site. Lower THC concentrations were generally observed at the soil surface (0-10 cm) when compared with higher THC concentrations obtained at higher soil depth (30 cm and 70cm). Concentration of residual THC at the soil surface (samples collected from the contaminated site) ranged between 1201 to 10046 mg/kg with a mean value of 5858.83 mg/kg; while the concentration of residual THC at subsurface soil (40 cm depth) ranged between 1016 to 11675 mg/kg with a mean value of 6374.50 mg/kg. These results show the relevance the oil companies to practice remediation follow-up in oil spill sites, in order to prevent remediation failure; leading to accumulation of residual hydrocarbon in the environment.
Lithological Investigation at Tombia and Opolo Using Vertical Electrical Soun...IJLT EMAS
Vertical electrical soundings (VES) was carried out in Opolo and Tombia all in Yenagoa local government area, Bayelsa state, Nigeria to understand the resistivity distribution of its subsurface which serves as a tool in investigating subsurface lithology. All VES sounding were stacked together to generate 1D pseudo tomogram and was subsequently interpreted. The interpreted VES curve results shows that Opolo consists of three layers within the depth of investigation. Sandy clay with mixture of silt make up the first layer (Top layer) with resistance value ranging from 24-63Ωm. The second layer is made up of thick clay with very low resistivity values ranging from 3-19Ωm. The third layer is sandyclay with its resistance value ranging from 26-727Ωm.Tombia also reveals that the area is in three layers within the depth of investigation. Sandy clay with a mixture of fine sand made up the first layer (Top soil) with its resistance values ranging from 40-1194Ωm. The second layer is made up of fine sand with resistivity value ranging from 475-5285Ωm. The third layer is made up of sandy clay/sand with its resistance value ranging from 24-28943Ωm.The results of the 1D pseudo tomogram also reveals that Tombia and Opolo consists of three layers within the depth of investigation and pseudo tomograms serves as a basis tool for interpreting lithology and identifying lithological boundaries for the subsurface
Public Health Implications of Locally Femented Milk (Nono) and Antibiotic Sus...IJLT EMAS
The study is to determine the PH and moisture content
of Nono sold in Port Harcourt , the prevalence of Pseudomonas
aeruginosa in Fura da nono and finally the antibiotic resistance
pattern of Pseudomonas aeruginosa isolated from the fermented
products. nono samples were purchased from Borikiri in
portharcourt township. A total of 20 samples were assessed to
determine their microbiological quality and to conduct antibiotic
susceptibility test. Moisture content and pH of the samples were
also assessed. Enumeration of the total viable bacterial count
(TVBC), Total coliform count (TCC) and Total Pseudomonal
count (TPC) were also assessed to determine the sanitary quality
of the product. The PH ranges between 2.99 to 3.89 while the
moisture content ranges between 80% to 88%. The result
obtained from the microbial culture indicated that a wide array
of microorganism were present in Fura da nono including species
of Bacilu, klebsiella, Pseudomonas Staphylococcus aureus,
Streptococcus, Lactobacillus and Escherichia coli.. The highest
TVBC, TCC and TPC were 9.8x103
cfu/ml, 10x103
cfu/ml and
9.7x103
cfu/ml respectively. Antibiotic susceptibility was
conducted using 12 broad spectrum antibiotics and compared
against a standard provided by the Clinical laboratory standard
institute (CLSI). Gentamycin, Ofloxacin and Levofloxacin
recorded 100% resistance , while Cotrimoxazole, Ciprofloxacin,
Vancomycin, Nitrofurantoin, Norfloxacin and Azithromycin
recorded 100% susceptibility as indicated by the complete clear
zone of inhibition.It was discovered that the absence of
regulatory agencies like National Agency for Food Drug
Administration and Control (NAFDAC) in the regulation of the
quality of the product was the cause of the high contamination,
since there were no quality control measures in its production
line .It was recommended that NAFDAC should provide a
standard operating procedure for local food producers and
should include them in their scope for regulation.
Comparison of Concurrent Mobile OS CharacteristicsIJLT EMAS
It is challenging for the mobile industry to supply the best features of the devices with its increasing customer requirements. Among the progress of technologies, the mobile industry is the fastest growing; as it keeps pace with rapidly changing market demands. This paper compares between the currently available mobile devices based on its user interface, security, memory utilization, processor, and device architecture. The mobile products launched from 2015-19 are used for comparison. Current results after comparison with earlier study found that many mobile devices and features became obsolete in a short time span supporting the aggressive growth of mobile industry.
Design of Complex Adders and Parity Generators Using Reversible GatesIJLT EMAS
This paper shows efficient design of an odd and even parity generator, a 4-bit ripple carry adder, and a 2-bit carry look ahead adder using reversible gates. Number of reversible gates used, garbage output, and percentage usage of outputs in implementing each combinational circuit is derived. The CLA used 10 reversible gates with 14 garbage outputs, with 50% percentage performance usage.
Design of Multiplexers, Decoder and a Full Subtractor using Reversible GatesIJLT EMAS
This paper shows an effective design of combinational circuits such as 2:1, 4:1 multiplexers, 2:4 decoder and a full subtractor using reversible gates. This paper also evaluates number of reversible gates used and garbage outputs in implementing each combinational circuit.
Multistage Classification of Alzheimer’s DiseaseIJLT EMAS
Alzheimer’s disease is a type of dementia that destroys
memory and other mental functions. During the progression of
the disease certain proteins called plaques and tangles get
deposited in hippocampus which is located in the temporal lobe
of brain. The disease is not a normal part of aging and gets
worsen over time. Medical imaging techniques like Magnetic
Resonance Imaging (MRI), Computed Tomography (CT) and
Positron Emission Tomography (PET) play significant role in the
disease diagnosis. In this paper, we propose a method for
classifying MRI into Normal Control (NC), Mild Cognitive
Impairment (MCI) and Alzheimer’s Disease(AD). An overall
outline of the methodology includes textural feature extraction,
feature reduction process and classification of the images into
various stages. Classification has been performed with three
classifiers namely Support Vector Machine (SVM), Artificial
Neural Network (ANN) and k-Nearest Neighbours (k-NN)
Design and Analysis of Disc Brake for Low Brake SquealIJLT EMAS
Vibration induced due to friction in disc brake is a
theme of major interest and related to the automotive industry.
Squeal noise generated during braking action is an indication of
a complicated dynamic problem which automobile industries
have faced for decades. For the current study, disc brake of 150
cc is considered. Vibration and sound level for different speed
are measured. Finite element and experimentation for modal
analysis of different element of disc brake and assembly are
carried out. In order to check that precision of the finite element
with those of experimentation, two stages are used both
component level and assembly level. Mesh sensitivity of the disc
brake component is considered. FE updating is utilized to reduce
the relative errors between the two measurements by tuning the
material. Different viscoelastic materials are selected and
constrained layer damping is designed. Constrained layer
damping applied on the back side of friction pads and compared
vibration and sound level of disc brake assembly without
constrained layer damping with disc brake assembly having
constrained layer. It was observed that there were reduction in
vibration and sound level. Nitrile rubber is most effective
material for constrained layer damping.
The aim of this article is to device strategies for
establishing and managing tomato processing industry, which
aims to enhance the taste experiences on different tomato
products for the people. Management needed for a successful
business is analyzed in each and every aspect. The five important
steps in management- planning, organizing, staffing, leading and
controlling are applied in management of the industry. Planning-
In the planning process, activities required to achieve desired
goals are thought about. This process involves the creation and
maintenance of a plan, those include psychological aspects that
require conceptual skills. Organizing- Organizing is a systematic
processing in order to attain objectives of structuring,
integrating, co-ordinating task, and activities. Staffing- Staffing is
the process of acquiring, deploying, and retaining a workforce of
sufficient quantity and quality to create positive impacts on the
organization’s effectiveness. Leading- Communicating,
motivating, inspiring and encouraging employees are key aspects
of process of leading, task of which is towards a higher level of
productivity of organization. Controlling- Controlling measures
the deviation of actual performance from the standard
performance, discovers the causes of such deviations and helps in
taking corrective actions.
This paper deals with the functioning of a Propylene
Recovery Unit (PRU) in a chemical industry and the various
Managerial and Human Resource considerations that need to be
accounted for, in this process. This report discusses various
aspects that are to be considered, before initializing the setup of
PRU, ranging from a Management perspective. Mission and
objective was decided and subsequently the managerial model
was developed. Propylene is an indispensible raw material that
has a variety of end use. A detailed analysis pertaining to
propylene demand in the market along with major sources has
been incorporated in this paper. Emphasis has been placed on
the type of departmentation required. Managerial aspects of
various functions ranging from warehousing to quality control
have also been taken into consideration. Delegations of functional
departments have been defined to prevent redundancy of duties
and major managerial functions of Planning, Organizing,
Staffing, Leading and Controlling has also been discussed.
Internal and External factors that affect the company have been
analyzed through SWOT Analysis and MBO strategies are also
broadly classified. Finally, Total Quality Management and
strategies for adoption of Lean Manufacturing as also touched
upon briefly.
This business model is intended to provide an online
platform connecting the general public customers with the
producers of groceries and food products such as fruits,
vegetables, meat and dairy products. The producers are selected
based on their production methods and their quality. The model
obtains the demand from the customers and the supply is found
from the producers. The prices of the products are fixed
according to the supply and demand. The customers' orders can
be classified into two different categories: 1. Bulk orders and 2.
Recipe based. The orders are obtained in a bulk quantity or for a
certain period of time and the products are delivered
periodically as per the customer's need. This model eliminates
the requirements of conventional storage units and also controls
the quality of the products using scientific devices. This model
reduces the wastage of resources as it enables the customer to
estimate their requirements using the help of recipe based
ordering system and also keeps the price constant for the bulk
orders.
Home textile exports are market driven, which implies that they deal with what the foreign market wants and how the home textile exporter could fulfil it, or product driven, where they deal with what the exporter has to offer and how can an appropriate strategy be applied to find the targeted buyers in the foreign market. The requisites of these are that the exporter must know the export plan, production procedure and export documentations. Exporter also must know his/her operational capacity, organizational nature and structure. An attempt is made in this project to understand and examine the nature and structure of the organization of the S3P exports.
Almost 80% of the population are coffee lovers.
Kaffinite sunshine café is guaranteed to become the daily
necessity for all the coffee addicts. A place with good ambience
where people can escape from their daily stress and cherish with
a morning cup of coffee. Our café offers home style delicious
breakfast and snacks. We focus on finding the most aromatic
and exotic coffee beans. We have our branches in many cities of
Tamil Nadu. We have a romantic ambience which attracts youth.
Our café has spectacular interior designs with stupendous taste
of coffee. We have attached our menu which contains multicuisines
at attractive prices. In this paper, we have done SWOT
analysis of our café to know our strengths and weaknesses. We
have also analyzed our opportunities and threats from the
external environment
Management of a Paper Manufacturing IndustryIJLT EMAS
This project focuses on how a paper manufacturing industry looks like and how it operates. For better understanding purpose, we have taken a hypothetical situation here. We have discussed on various factors that are to be considered before constructing a plant. For example, what kind of proprietorship is suitable for this case? We have developed a SWOT Analysis for the plant, thinking about the pros and cons. This project can be a guide for a person who is willing to start up a new manufacturing plant. This report can be used to streamline your approach to planning by outlining the responsibilities of plant managers and external factors, as well as identifying appropriate resources to assist you with the construction of plant.
Application of Big Data Systems to Airline ManagementIJLT EMAS
The business world is in the midst of the next
revolution following the IT revolution – the Big Data revolution.
The sheer volume of data produced is a major reason for the big
data revolution. Aviation and aerospace are typical areas that
can apply big data systems due to the scale of data produced, not
only by the plane sensors and passengers, but also by the
prospective passengers. Data that need to be considered include,
but are not limited to, aircraft sensor data, passenger data,
weather data, aircraft maintenance data and air traffic data.
This paper aims at identifying areas in aviation where big data
systems can be utilized to enhance operational performances
improve customer relations and thereby aiding the ultimate goal
of increased profits at reduced costs. An improved management
model built on a strong big data infrastructure will reduce
operation costs, improve safety, bring down the cost and time
spent on maintenance and drastically improve customer
relations.
Impact of Organisational behaviour and HR Practices on Employee Retention in ...IJLT EMAS
I. INTRODUCTION
Roads are constituted as the most significant component of
India‟s Logistics Industry, accounting for 60 percent of
the total freight movement in the country. A majority of
players in this industry are small entrepreneurs running their
family businesses. As a result, Man Power Development
Investments that pay off in the longer term, have been
minimised respectively. Moreover, these businesses are
typically controlled severely by the proprietor and his / her
family and consequently, making it unattractive for the
professionals. Poor working conditions, Low pay scales
relative to alternate careers, poor or non-existent Manpower
Policies and prevalence of unscrupulous practices have added
to the segment's woes for seeking employment. Thus, it could
be rightly stated that the Transportation, Logistics,
Warehousing and Packaging Sector is considered an
unattractive career option and fails to attract and retain skilled
manpower. Many Organizations have failed to recognize that
Human Resources play an important role in gaining an
immense advantage in today‟s highly competitive Global
Business Environment. While all aspects of managing Human
Resources is important, Employee Retention continues to be
an essential part of Human Resource Management activity
that help the Organizations to achieve their goals and
objectives.
Sustainable Methods used to reduce the Energy Consumption by Various Faciliti...IJLT EMAS
The purpose of this article is to identify the energy
challenges faced by airports especially with regards to the energy
consumed by the terminal building and suggest suitable energy
conservation techniques based on what has already been
implemented in few airports around the world.
We have identified the various facilities and systems which are
responsible for a major share of the consumption of energy by
airport terminals and we have suggested measures to effectively
overcome these problems.
OVERVIEW OF THE COMPANY
Cake Walk sweets and savories
Cake Walk is India‟s No. 1 confectionery and cake
manufacturer with its products exported to over 20 countries
around the world. They are dedicated to the art of producing
innovative and delicious products for sweet lovers of all ages.
Cake Walk‟s products offer tantalizing experiences that sparks
the imagination in people who eat their candy. Of course, this
has been Cake Walk‟s goal since their inception in 1947.
Today, Cake Walk Candy continues to make some of the best
candy in India. They also are a responsible business venture
and contribute positively to the society with their “Learn to
bake” initiative to encourage households to earn by starting
their own small-scale businesses. Cake Walk products can be
enjoyed by kids and adults alike, and their products come in
an array of flavors, shapes and sizes.
Every individual in our planet is busy in his / her own
world these days. The busy schedules and work preoccupations
of many people hinder them from spending nominal amount of
time with their families.
To address this concern, we have come up with our MACH
Tours and Travels, our motto being, “Breaching the
Boundaries!” which aims at not only giving its customers the best
and most comfortable tour, but also an enjoyable and
memorable experiences.
We differ from our competitors in various ways. For a start, we
emphasize that our profit is not in the income from this business,
but in the satisfaction of our customers. Added to that, we focus
on improving the ease of travel, the luxury of trip, the quality of
time spent and the worth of pay.
There is a variety of customers we come across: some will want
their trip to be extravagant, while some require it to be cost
effective; some need a long vacation, while some choose just a
weekend away.
Our mission: In order to meet the desires of this large range of
people and to include all the factors of a hearty holiday, we have
devised our strategies and planned our processes, thus, setting us
apart from the others.
Our vision: As the main priority, a year from now, we target on
contenting as many customers as possible through our services.
The following sections of this document includes our roles in
planning, decision making, staffing, leading and communicating
in which we highlight various aspects of our organization,
including the pros and cons of travelling with us.
The purpose of this paper is to highlight the general
terms and definitions that falls under the ‘common set’ in the
intersection of the sets Meteorology and Aerospace Engineering.
It begins with the universal explanations for the meteorological
phenomena under the ‘common set’ followed by the
categorization of clouds and their influences on the aerial
vehicles, the instrumentation used in Aeronautics to determine
the required Meteorological quantities, factors affecting aviation,
effects of aviation on the clouds, and the corresponding protocols
involved in deciphering the ‘common set’ elements.
It also talks about the relation between airport construction and
Geology prior to concluding with the uses and successes of
Meteorology in the field of Aerospace.
A Study on Impact of Internal Mobility on Organisational Performance: A Case ...IJLT EMAS
Over a time, manufacturing Industry in India is essentially an outstanding amongst the most well-known enterprises in Indian market. Manufacturing is the producing of goods and a service using labor and other machinery tools this helps to understand the organization performance directly or indirectly has an impact on internal mobility of an employee. Internal mobility mainly tells that relying upon worker's expertise skill and prerequisites with the toss of the employment with organizational commitment and the change in job structure, job design occurs with or without raise in the salary. Organization performance which has many factors which influence the performance but internal mobility is also a supporting factor which has an impact in achieving the vision of the company. This gives an understanding that apart from providing all the necessary facility and good working culture, healthy promotion and transfer employee exit rates are increasing. Thus, internal mobility is most preferable objective to success which implies observable career accomplishments such as promotion, transfer & hierarchical level held and salary. In future to achieve internal mobility, company should adapt the principle of succession management at all ranks, provide transparent discussion of skill and the potential, as well as organizational needs. The reason for this would the favoritism and lack of recognition which is making an employee dissatisfaction towards their organization by this I conclude that the unfair or unethical practices in organization with respect to the internal mobility in the future periods make employee unhappy this is one of the reason to leave an organization which creates a huge impact in the organization performance
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Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
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Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
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Micro RNA genes and their likely influence in rice (Oryza sativa L.) dynamic ...Open Access Research Paper
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Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
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"Understanding the Carbon Cycle: Processes, Human Impacts, and Strategies for...MMariSelvam4
The carbon cycle is a critical component of Earth's environmental system, governing the movement and transformation of carbon through various reservoirs, including the atmosphere, oceans, soil, and living organisms. This complex cycle involves several key processes such as photosynthesis, respiration, decomposition, and carbon sequestration, each contributing to the regulation of carbon levels on the planet.
Human activities, particularly fossil fuel combustion and deforestation, have significantly altered the natural carbon cycle, leading to increased atmospheric carbon dioxide concentrations and driving climate change. Understanding the intricacies of the carbon cycle is essential for assessing the impacts of these changes and developing effective mitigation strategies.
By studying the carbon cycle, scientists can identify carbon sources and sinks, measure carbon fluxes, and predict future trends. This knowledge is crucial for crafting policies aimed at reducing carbon emissions, enhancing carbon storage, and promoting sustainable practices. The carbon cycle's interplay with climate systems, ecosystems, and human activities underscores its importance in maintaining a stable and healthy planet.
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Bioremediation Potentials of Hydrocarbonoclastic Bacteria Indigenous in the Oil Impacted Sites of Ogoniland, Rivers State, Nigeria
1. International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume IV, Issue VIII, August 2019|ISSN 2454-6194
www.rsisinternational.org Page 107
Bioremediation Potentials of Hydrocarbonoclastic
Bacteria Indigenous in the Oil Impacted Sites of
Ogoniland, Rivers State, Nigeria
Wiri, .T.B, Desmond,.O., Mbato,.CI., Nabiradee, P.N.
Dept of Environmental Health, Rivers State College of Health Science and Management Technology Port Harcourt, Rivers State,
P.M.B.5039 Nigeria
Abstract:-Hydrocarbon pollution Remediation by Enhanced
Natural Attenuation method was adopted to remediate the
hydrocarbon impacted site in Ogoniland Rivers State, Nigeria .
The research lasted for 6 months. Samples were collected at
monthly intervals . samples were collected intermittently
between Feb 2019 to July 2019 . Mineral salt medium containing
crude oil was used as a sole source of carbon and energy for the
isolation of hydrocarbonoclastic bacteria. Samples were
collected from the four (4) local government that made up
Ogoniland and they includes Khana(k), Gokana (G),Tai (T),
Eleme (E) and transported immediately to the laboratory for
analysis. The microbial and physicochemical properties of the
soil samples varied with the different local government areas.
Seven bacteria genera were isolated from the samples from the
four locations, viz, Pseudomonas, Lactobacter, Micrococcus,
Arthrobacter, Bacillus, Brevibacterium and Mycobacterium
were isolated and identified. the seven isolate were indigenous in
the study area. Nutrient were added to identified plots of
hydrocarbon pollution polluted site within the four local
government and they were able degrade hydrocarbon within a
short of period of time. Reassessment of physicochemical
parameter impacted site was used to judge the bioremediation
potentials of microorganism.
Key words:- Bioremediation, Hydrocarbonoclastic, Bacteria,
potentials, Ogoniland, Rivers state
I. INTRODUCTION
ydrocarbon pollution in ogoniland is a global issue,the
land has been polluted with hydrocarbon which has
greatly affected health and agricultural activities of the people,
The extent of the pollution warranted the united nation
environmental programme (UNEP) recommended the clean
up of ogoniland.
Petroleum is at present Nigeria’s and indeed, the world’s most
important derived energy source ((Moffat and Linden, 2005;
Nigerian Environmental Study Action Team, 2006). However,
the growth and activities of petroleum and petroleum
associated industries in Nigeria and in other parts of the world
has led to increased oil pollution in our environment.
Petroleum in its natural state is called crude oil (Ukoli, 2003).
The greatest single environmental problem connected with
crude oil exploration in Nigeria is oil spillage both on-shore
and off-shore. The rate of oil spillage reported in the country
has been rising with a corresponding increase in petroleum
production.
The various genera that have been reported to contain
hydrocarbon-degrading species include Pseudomonas, Vibrio,
Corynebacterium, Arthrobacter, Brevibacterium,
Flavobacterium, Sporobolomyces, Achromobacte, Bacillus,
Aeromonas, Thiobacillus, Lactobacter, Staphylococcus,
Penicillium and Articulosporium. These organisms have been
isolated in large numbers from many oil polluted waters and
soils, but are found in less numbers in uncontaminated
environments.
Many studies have been conducted to isolate and characterize
hydrocarbon degraders from oil spill sites but little have been
done to determine the changes in soil mineral nutrients and
total petroleum hydrocarbon as bioremediation of the spill site
progresses. This study will provide information on the
effectiveness of microorganisms in eliminating oil pollutants
from the oil polluted areas of the Niger delta region in
Nigeria. Specifically, the experiment was designed to; isolate
and characterize hydrocarbon utilizing bacteria and fungi from
crude oil impacted soil samples and also determine the extent
to which the spilled crude oil has been degraded.
II. MATERIALS AND METHODS
Collection of Soil Samples
Soil contaminated samples used for the study were obtained
from oil impacted sites in Khana, Gokana, Tai and Eleme
Local Government Area of Rivers State, Nigeria. The sample
were obtained between Feb 2019 to July 2019. The samples
were collected aseptically using soil sampler to a depth of 20
cm, stored in sterile aluminum foils and transported to the
laboratory immediately for analysis. The sample were coded
using the first alphabet of the local government : K,T,G and E
respectively.
Enumeration of total heterotrophic bacteria and in the soil
samples 1 g of each sample was serially diluted (10-1
to 10-5
).
And were plated in duplicate on sterile nutrient agar plates,
using the pour plate method at 37°C for 24 h for the nutrient
agar plate, however colonies who shows slow growth rate
H
2. International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume IV, Issue VIII, August 2019|ISSN 2454-6194
www.rsisinternational.org Page 108
were further allow for 48h at 37° and were afterwards
enumerated
Enumeration of Hydrocarbonoclastic Bacteria
1 ml from dilutions of 10-5
were plated in duplicate on pre
dried mineral salt agar using the spread plate technique, the
10-3 and 10-4 dilutions were plated . A filter paper saturated
with sterile crude oil was aseptically placed on the inverted
Petri dishes and the culture plates were incubated for 72h at
37°C . Plates yielding significant colonies were enumerated .
Isolation and Characterization of Hydrocarbon-Utilizing
Bacteria
Colonies of different hydrocarbon eating bacteria were
selected at random using a sterile wire loop and subcultured,
by streaking on nutrient agar plates to obtain pure colonies.
All the isolates were characterized using the techniques of
Chesborogh (2000)
Determination of Physicochemical Properties of
Contaminated Soil
Analyses of physicochemical properties of the soil samples
were performed according to the American Society ASTM
(1998).
Determination of Total Petroleum Hydrocarbon
FTI Spectrophotometer was used for the test. Prior to analysis
the soil samples were extracted with carbon tetrachloride and
treated with 2% deactivated silica gel. The equipment was
calibrated with isooctane/octane in carbon tetrachloride. TPH
concentrations in the samples were determined by using the
stored calibration graphmoisture Content determination
A weighed amount of the soil sample was placed in a weighed
crucible and dried at 105°C in the oven until a constant weight
was reached. From the difference in weight, the percentage
moisture content was calculated.
Phosphate Content Determination
Samples were extracted with 25% acetic acid and the extract
run on the Unicam UV/visible spectrophotometer at a
wavelength of 700 nm. A spike sample was analyzed in every
batch of analysis. A standard was analyzed after every batch
of samples and the first value of the standard was used to plot
the means control chart.
Nitrate and pH Determination
Soil samples were extracted with sodium acetate in the
presence of sulphuric acid and measured at a wavelength of
470 nm using Unicam UV/visible spectrophotometer. To
assess the pH, electrodes of a multimeter were dipped into a
mixture of soil sample and deionized water. The pH values of
the samples were subsequently read on the multimeter.
Statistical Analysis of Data
All the data obtained were subjected to statistical analysis of
variance (ANOVA) using SPSS program.
III. RESULTS
Microbial Counts and Identification
Hydrocarbonoclastic bacteria isolated were, Brevibacterium
Lactobacter, Arthrobacter, Bacillus, Pseudomonas,
Mycobacterium and Micrococcus were isolated and identified.
TABLE 1: TOTAL HETEROTROPHIC BACTEIA THB
SAMPLE DILLUTIONS 10-3
10-4
KHANA (K) 3.0X105
2.7X105
GOKANA (G) 2.41X105 1.7X105
TAI (T) 1.90X105 1.5X105
ELEME (E) 2.30X1O5 2.50X105
TABLE 2 HYDROCARBONOCLASTIC BACTERIA ISOLATED FROM
THE FOUR LOCATIONS
SAMPLE
ISOLATES K G T E
Mycobacterium + + + +
Pseudomonas + + + +
Lactobacter, + + + +
Micrococcus + + + +
Arthrobacter + + + +
Bacillus + + + +
Brevibacterium + + + +
TABLE 3. PHYSICOCHEMICAL PARAMETER OF OIL POLLUTED
SOILBEFORE BIOREMEDIATION
Parameter Sample Location
PH
K G T E
7.00 7.01 6.50 6.90
Nitrogen 0.28mg/kg
0.26mg/k
g
0.31mg/k
g
0.33mg/
kg
Phosphorus 0.33mg/kg
0.35mg/k
g
0.30mg/k
g
0.34mg/
kg
Total Organic
Carbon (TOC)
0.8% 0.7% 0.8% 0.7%
Total hydrocarbon
content (THC)
2,845mg/g
2,885mg/
g
2,950mg/
g
2,740mg
/g
Total Petroleum
Hydrocarbon
(TPH)
3,475mg/g
3,375mg/
g
3,500mg/
g
3,380mg
/g
Polycyclic
Aromatic
Hydrocarbon(PAT
H)
15.65mg/g
15.40mg/
g
15.35mg/
g
15.29mg
/g
TABLE 3. PHYSICOCHEMICAL PARAMETEROF OIL POLLUTED
SOIL AFTER BIOREMEDIATION
PARAMETER SAMPLE LOCATION
PH
K G T E
4.00 4.01 4.50 3.90
Nitrogen
5.01g/k
g
4.45mg/kg 3.22mg/kg
2.9.5mg/k
g
Phosphorus
0.55mg/
kg
0.50mg/kg 0.70mg/kg 0.61mg/kg
3. International Journal of Research and Innovation in Applied Science (IJRIAS) | Volume IV, Issue VIII, August 2019|ISSN 2454-6194
www.rsisinternational.org Page 109
Total Organic
Carbon (TOC)
115.5% 100.5% 200.1% 185.7%
Total
hydrocarbon
content (THC)
2,845m
g/g
2,885mg/g 2,950mg/g 2,740mg/g
Total
Petroleum
Hydrocarbon
(TPH)
70.5mg/
g
75.5mg/g 80.7mg/g 68.0mg/g
Polycyclic
Aromatic
Hydrocarbon
(PATH)
2.65mg/
g
3.40mg/g 3.35mg/g 4.29mg/g
IV. DISCUSSION
The results of the study indicated that the total heterotrophic
bacteria (THB) counts varied over a period of time. Result
shows significant differences in physicochemical parameter
before and after bioremediation. The differences in the
physicochemical parameter was use to ascertain the degree or
potentials of hydrocarbon utilizing effects of the isolated
microorganism. The counts of hydrocarbonoclastic bacteria
were higher in hydrocarbon polluted soil than non impacted
soil, this may be as a result of the fact the hydrocarbon
provides a source of carbon and nitrogen needed for the
proliferation of the hydrocarbonoclast. The pH values of
crude oil polluted soil were lower as compared to those of
crude oil free soil. The decrease in pH value may be due to
increased degradation of crude oil by microorganisms in the
soil, res. Both nitrogen and phosphorus levels were high in
hydrocarbon impacted soil than oil free soil. This agrees with
the finding of (A.K. Onifade and F.A. Abubakar (2007)), who
reported increase in nitrogen and phosphorus contents of a
crude oil polluted soil.
Conclusively, the study shows that there was residual crude
oil in the soil after 6 months of research. The study also shows
that the physiochemical properties of the soil were
significantly affected. Finally, the study has proven that
Ogoniland has indeginous microbial population that has the
potentials to remediate hydrocarbon pollution.
REFERENCES
[1]. ASTM, 1998.Standard Guide for Remediation of Soil by Natural
Attenuation at Petroleum Release Sites E-1943-98.ASTM, West
Conshohocken, Pennsylvania.
[2]. Atlas, R.M., 1995. Bioremediation of petroleum pollutants. Int.
Biodeteriorat. Biodegradat., 35: 317-327.
[3]. Ijah, J.J. and O.P. Abioye, 2003.Assessment of physicochemical
and microbiological properties of soil 30 month after kerosene
spill. J. Res. Sci. Manage., 1: 24-30.
[4]. A.K. Oniade and F.A.Abubakar (2007) characterization of
hydrocarbon degrading microorganism isolated from crude oil
contaminated soil and remediation by natural attenuation .journal
of microbiology, 2:149-155