This study characterized bacteria in the rhizosphere of various plants found growing in a petroleum-polluted soil in Nigeria. Bacteria from the genera Acinetobacter, Bacillus, Micrococcus, and Pseudomonas were isolated from the rhizosphere of plants like Mimosa pudica and Alchornia chordifolia. These bacteria were able to grow on petroleum hydrocarbons. Bacterial counts were higher in the polluted rhizosphere compared to the non-rhizosphere, indicating a positive rhizosphere effect. The isolated rhizobacteria could potentially be used to bioremediate crude oil pollution based on their ability to degrade hydrocarbons. The plants may also assist rhizoremedi
Bioremediating Effect of Glomus Hoi and Pseudomonas Aeruginosa on the Organic...IJEAB
This study analyzed the degrading effect of Glomus hoi and Pseudomonas aeruginosa on the organic content and heavy metals of oil refinery effluent polluted soil using Amaranthus cruentus as the test plant. This study was carried out to determine if agricultural activities can be improved using any or both of the micoorganisms. Eight different treatment layouts were used with three replicates for each level of pollution in the treatment layout. Ninety six (96) pots, each containing three kilograms of soil from both sterilized and unsterilized soil were used for the study. Fifty (50) grams of soil inoculum from propagated Arbuscular mycorrhiza was inoculated to a set of twenty four (24) experimental pots containing both sterilized and unsterilized soil before A. cruentus seedlings were transplanted to them. Another set of twenty four (24) pots containing both sterilized and unsterilized soil were injected with thirty (30) mL of P. aeruginosa inoculum solution before transplanting A. cruentus seedlings to them. The third set of twenty four (24) pots received dual inoculation of both fifty (50) grams of soil inoculum containing G. hoi and thirty (30) mL of P. aeruginosa inoculum solution before A. cruentus were transplanted to them. The residual twenty four (24) pots served as the control. Thereafter, pot preparation was arranged in the screenhouse in a randomized block design. The A. cruentus seedlings were raised in nursery for a period of two weeks before they were transplanted to the pots, seedlings were left for 3 days to overcome transplanting shock before contaminating the soil with refinery effluent at various concentrations of 0%, 2%, 4% and 6% v/w. The seedlings were allowed to grow for eight weeks before the termination of the experiment. The pre planting analysis of soil showed that heavy metals analyses (zinc and iron) of sterilized soil had a lower concentration to the unsterilized. The soil pH ranged from 6.3 to 6.8. It also revealed that organic matter and organic carbon content ranged from 0.8% to 1.3% and 0.4% to 1.7%. However, after the experiment, it was discovered in this study that treatments without any microorganism inoculation in sterilized and unsterilized soil had a higher level of % organic carbon and % organic matter content compared to the other treatments that were inoculated with one or two micro-organisms across all the levels of effluent concentration. Heavy metals of soil in all the soil samples were found to increase as the petrochemical effluent increased in concentration. The results obtained were analyzed using Duncan Multiple Range Test (DMRT) and other descriptive statistics. This study opined that the combined use of G. hoi and P. aeruginosa was more effective in improving the organic contentand the reduce heavy metals of oil refinery effluent polluted soil than when either is used singly.
Microbiological and physicochemical analyses of top soils obtained from four ...Innspub Net
Several methodologies were utilized to evaluate the microbiological and physico chemical properties of top soil samples bored from four municipal waste dumpsites and a farmland (control sample) all located in Benin City, Edo State. The soil samples were obtained during the month of January, 2013. The mean aerobic bacterial counts for the soil samples ranged from 9.7 × 103 cfu/g for the control soil to 1.80 × 104 cfu/g for the soil sourced from the dump site at Ikheuniro. The mean heterotrophic fungal counts varied from 7.0 × 102 cfu/g for capitol dumpsite to 3.3 × 103 cfu/g for the control soil. Ten (10) microbial isolates were characterized and identified; Bacillus sp., Pseudomonas sp., Aeromonas sp., Enterobacter sp., Klebsiella sp. and Staphylococcus sp., Aspergillus sp., Mucor sp., Saccharomyces sp. and Fusarium sp. respectively. Both Bacillus sp. and Pseudomonas sp. were the most dominant amongst the bacterial isolates whilst Staphylococcus sp. was the least occurring bacterial isolate. Aspergillus sp. was the highest occurring fungal isolate while the least isolated fungal culture was Saccharomyces sp. The physico chemical results showed values which ranged from 5.60 to 8.08, 164.00 µS/cm to 540.00 µS/cm, 2.378 mg/kg to 3.444 mg/kg, 0.009 mg/kg to 0.016 mg/kg for pH, electrical conductivity, sulphate and cadmium. Despite the positive impacts of the dumped municipal wastes on the microbial and organic properties of the analyzed soils, disposal of municipal wastes in open dump sites is an archaic and unsustainable option in the management of municipal wastes. Get the full articles at: http://www.innspub.net/volume-1-number-1-september-2013/
Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soilijtsrd
In-vivo experiment was conducted for toxicity evaluation of Imidacloprid on soil enzyme activities arylsulphatase, acid-phosphatase and dehydrogenase activities in the treated soil under control condition for 60 days at different application rate 3.97µg mL FR , 7.94µg mL 2FR and 39.7µg mL 10FR . Imidacloprid has significant toxic at 2FR and 10FR doses but not on the recommended field rate FR . Acid phosphatase activity was stimulated at FR rate, whereas at higher dose 10FR inhibited the activity. A significant drop of dehydrogenase activity was observed irrespective of doses at 30 days of application and after that the enzymatic activity slowly increased. Application of Imidacloprid at FR to agriculture soil of Tripura is not likely to show any detrimental effects on soil acid phosphatase and dehydrogenase activities but at higher elevated doses there appear some harmful effects which was also very much transient. Aloke Purkait | Dipak Kumar Hazra | Pabitra Kumar Biswas | Ashim Chowdhury "Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21406.pdf
Paper URL: https://www.ijtsrd.com/chemistry/analytical-chemistry/21406/studies-the-effects-of-imidacloprid-on-enzymatic-activities-in-clay-loam-soil/aloke-purkait
Bioremediating Effect of Glomus Hoi and Pseudomonas Aeruginosa on the Organic...IJEAB
This study analyzed the degrading effect of Glomus hoi and Pseudomonas aeruginosa on the organic content and heavy metals of oil refinery effluent polluted soil using Amaranthus cruentus as the test plant. This study was carried out to determine if agricultural activities can be improved using any or both of the micoorganisms. Eight different treatment layouts were used with three replicates for each level of pollution in the treatment layout. Ninety six (96) pots, each containing three kilograms of soil from both sterilized and unsterilized soil were used for the study. Fifty (50) grams of soil inoculum from propagated Arbuscular mycorrhiza was inoculated to a set of twenty four (24) experimental pots containing both sterilized and unsterilized soil before A. cruentus seedlings were transplanted to them. Another set of twenty four (24) pots containing both sterilized and unsterilized soil were injected with thirty (30) mL of P. aeruginosa inoculum solution before transplanting A. cruentus seedlings to them. The third set of twenty four (24) pots received dual inoculation of both fifty (50) grams of soil inoculum containing G. hoi and thirty (30) mL of P. aeruginosa inoculum solution before A. cruentus were transplanted to them. The residual twenty four (24) pots served as the control. Thereafter, pot preparation was arranged in the screenhouse in a randomized block design. The A. cruentus seedlings were raised in nursery for a period of two weeks before they were transplanted to the pots, seedlings were left for 3 days to overcome transplanting shock before contaminating the soil with refinery effluent at various concentrations of 0%, 2%, 4% and 6% v/w. The seedlings were allowed to grow for eight weeks before the termination of the experiment. The pre planting analysis of soil showed that heavy metals analyses (zinc and iron) of sterilized soil had a lower concentration to the unsterilized. The soil pH ranged from 6.3 to 6.8. It also revealed that organic matter and organic carbon content ranged from 0.8% to 1.3% and 0.4% to 1.7%. However, after the experiment, it was discovered in this study that treatments without any microorganism inoculation in sterilized and unsterilized soil had a higher level of % organic carbon and % organic matter content compared to the other treatments that were inoculated with one or two micro-organisms across all the levels of effluent concentration. Heavy metals of soil in all the soil samples were found to increase as the petrochemical effluent increased in concentration. The results obtained were analyzed using Duncan Multiple Range Test (DMRT) and other descriptive statistics. This study opined that the combined use of G. hoi and P. aeruginosa was more effective in improving the organic contentand the reduce heavy metals of oil refinery effluent polluted soil than when either is used singly.
Microbiological and physicochemical analyses of top soils obtained from four ...Innspub Net
Several methodologies were utilized to evaluate the microbiological and physico chemical properties of top soil samples bored from four municipal waste dumpsites and a farmland (control sample) all located in Benin City, Edo State. The soil samples were obtained during the month of January, 2013. The mean aerobic bacterial counts for the soil samples ranged from 9.7 × 103 cfu/g for the control soil to 1.80 × 104 cfu/g for the soil sourced from the dump site at Ikheuniro. The mean heterotrophic fungal counts varied from 7.0 × 102 cfu/g for capitol dumpsite to 3.3 × 103 cfu/g for the control soil. Ten (10) microbial isolates were characterized and identified; Bacillus sp., Pseudomonas sp., Aeromonas sp., Enterobacter sp., Klebsiella sp. and Staphylococcus sp., Aspergillus sp., Mucor sp., Saccharomyces sp. and Fusarium sp. respectively. Both Bacillus sp. and Pseudomonas sp. were the most dominant amongst the bacterial isolates whilst Staphylococcus sp. was the least occurring bacterial isolate. Aspergillus sp. was the highest occurring fungal isolate while the least isolated fungal culture was Saccharomyces sp. The physico chemical results showed values which ranged from 5.60 to 8.08, 164.00 µS/cm to 540.00 µS/cm, 2.378 mg/kg to 3.444 mg/kg, 0.009 mg/kg to 0.016 mg/kg for pH, electrical conductivity, sulphate and cadmium. Despite the positive impacts of the dumped municipal wastes on the microbial and organic properties of the analyzed soils, disposal of municipal wastes in open dump sites is an archaic and unsustainable option in the management of municipal wastes. Get the full articles at: http://www.innspub.net/volume-1-number-1-september-2013/
Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soilijtsrd
In-vivo experiment was conducted for toxicity evaluation of Imidacloprid on soil enzyme activities arylsulphatase, acid-phosphatase and dehydrogenase activities in the treated soil under control condition for 60 days at different application rate 3.97µg mL FR , 7.94µg mL 2FR and 39.7µg mL 10FR . Imidacloprid has significant toxic at 2FR and 10FR doses but not on the recommended field rate FR . Acid phosphatase activity was stimulated at FR rate, whereas at higher dose 10FR inhibited the activity. A significant drop of dehydrogenase activity was observed irrespective of doses at 30 days of application and after that the enzymatic activity slowly increased. Application of Imidacloprid at FR to agriculture soil of Tripura is not likely to show any detrimental effects on soil acid phosphatase and dehydrogenase activities but at higher elevated doses there appear some harmful effects which was also very much transient. Aloke Purkait | Dipak Kumar Hazra | Pabitra Kumar Biswas | Ashim Chowdhury "Studies the Effects of Imidacloprid on Enzymatic Activities in Clay Loam Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-2 , February 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21406.pdf
Paper URL: https://www.ijtsrd.com/chemistry/analytical-chemistry/21406/studies-the-effects-of-imidacloprid-on-enzymatic-activities-in-clay-loam-soil/aloke-purkait
ABSTRACT- The development of human civilization throughout history has led to growing disruption of the natural
balance and the occurrence of different types of pollution. Environmental pollution with petroleum and petrochemical
products has been recognized as significant and serious problem. Diesel engine oil, which is one of the major products of
crude oil, constitutes a major source of pollution in our environment. Therefore diesel engine oil can enter into the
environment through wrecks of oil tankers carrying diesel oil, cleaning of diesel tanks by merchants, war ships carrying
diesel oil and motor mechanics. In present study the microorganisms utilising petrol and diesel oil as carbon source were
isolated and investigation of their characteristics towards the production of polyhydroxyalkanoates (PHA), which is now a
days well known as biodegradable polymer.
Key Words- Petrol and Diesel oil contamination, Bioremediation, Biodegradable bacterial polymer, Sudan
Black B staining, 16sr RNA sequencing
Screening of Biosurfactant Bioemulsifier Producing Bacteria from Petroleum Co...ijtsrd
The release of impurities in the environment, containing petroleum and petroleum cogitated products, is engenders of global being taint. It is also a hazardous for human and animal health, since many of these impurities have evidenced to be toxic and oncogenic. Hydrocarbon particles that are secreted into the environment are hard to get rid of, since they change state to surfaces and are captured by surface tension in a water immiscible stage. Bioremediation has tested to be an alternate to lessen the effects caused to impureness of soil and water, applying the metabolic abilities of microorganisms that can apply hydrocarbons as source of carbon and energy, or that can alter them by co metabolism. The proficiency of removal is directly related to the compound’s chemical structure, to its bioavailability deliberation, harmfulness, flexibility and approach and to the physicochemical situation present in the atmosphere. Perwez Qureshi | Dr. Reshma Jaweria "Screening of Biosurfactant/Bioemulsifier Producing Bacteria from Petroleum Contaminated Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46276.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/46276/screening-of-biosurfactantbioemulsifier-producing-bacteria-from-petroleum-contaminated-soil/perwez-qureshi
A study on physiological, anatomical characterization of selected carrot plan...Innspub Net
Carrots were first used for medical purposes and gradually used as food. It is also a good source of magnesium and manganese. Cadmium is a nonessential element that adversely affects plant growth and development. It is considered as one of the significant pollutants due to its high toxicity and more solubility in water. Experiment was conducted in Old Botanical Garden of University of Agriculture Faisalabad to check the response of carrot genotypes grown under Cadmium Chloride stress (0µM, 10µM, 15µM and 20µM). Variety of carrot (gajar) used was Red Gold. Seeds were sown in 12 pots, which were filled with sand, in 2nd week of November. Germination observed after one week of sowing. The experiment was laid out in a completely randomized design with three replicates. Seeds of carrot (red gold) were purchased from Ayyub Agriculture Research Institute (AARI) Faisalabad and sown directly in the plastic pot. 8 seeds per pot were distributed. Hogland solution was also applied to replicates and then I applied Cadmium Chloride stress to the plants to start my experiment. Cadmium stress decreased the uptake and distribution of essential elements in plant. Studies had revealed that heavy metals cause adverse effects on plant growth, which further lead to decrease plant yield and inhibition of enzymatic activities.In the present study, plant growth characteristics, root length and shoot length decreased under Cd stress. In fact, salt stress decreased all attributes in carrot when the concentrations of Cadmium chloride increased higher and higher.
Dissertation ppt biostimulation- a potential practice for wastewater treaat...Sumer Pankaj
Phycoremediation is a green technology that supports the direct use of living green microalgae for in situ, or in place removal, degradation, of contaminants in soils, sludge, sediments, surface water and ground waters by the mechanisms of bio-transformation, bio-accumulation, bio-concentration, bio-sparging.
It can be said by the current study that microalgae has a great potential for the treatment of industrial and municipal wastewaters as compared to the chemical treatments available commercially. Biological systems are much more efficient in cleaning the excess nutrients from the waste water followed by generation of valuable biomass which can be applied in the food, fertilizer, energy production as use of inorganic chemicals like lime and ferrous sulphate generates huge amount of sludge in textile industries, but on the other hand static anaerobic treatment using acclimatized MLSS gives better colour reduction with zero sludge generation. Microalgal cells can be used in free form to treat waste waters containing high C.O.D., high ammonical nitrogen and high TDS. It not only provides a better reduction of chemicals from wastewaters but it also helps to reduce the operational cost of ETP. Microalgaes not only helps to remediate industrial waste waters but also to treat sweage water and to restore natural water bodies like lakes and ponds. As they are active in remediating the chemicals but also it shows an antagonistic effect against some pathogenic germs like total coliforms and fecal coliforms.
These microalgal cells can also be combined with bacterial biomass of activated sludge process to develop an Algal-Bacterial consortium (ALBA) for better enhancement in the reduction of chemicals from the wastewaters as this symbiotic relation of algae and bacteria provides high satiability of the microalgae along with MLSS and faceable in terms of price and economy for instance the bacterial biomass provides carbon dioxide to algal cells for photosynthesis and in return the bacteria acquires oxygen from algae. The harvested biomass from the ETP’s can be used as bio-fertilizers as it consists of appropriate ratio of vital macro and micro nutrients like N,P,K etc. which enhance the growth of plantlets. It can also be used as aqua feeds for shrimps, fishes and molluscs. Furthermore these microlgal cells are non-toxic in the environment as it becomes a part of food chain and do not cause eutrophication. Therefore, micro-algal based treatment is most suitable for the treating the waste waters and restoring the natural water bodies as compared to other chemical treatments.
Isolation and Screening for Citric Acid Production by Aspergillus Niger Using...ijtsrd
The numerous applications of citric acid have increased its demand. It is of great importance to produce citric acid using adequate monosaccharides and dissacharides as they have proven to be easily catabolyzed and decomposed by Fungi when compared to polysaccharides. The superiority of sucrose to other sugars is a result of strong extracellular mycelium bound invertase of A.niger which rapidly hydrolyses sucrose at low pH. This project was undertaken to isolate, screen and produce citric acid in a submerged fermentation by four different strains of Aspergillus niger using sucrose. Aspergillus niger was isolated and characterized from garden soil. Citric acid production from Aspergillus niger in a submerged basal medium was quantitatively determined using titrimetric method. Aspergillus niger strain HUS1 ASHUS1 , Aspergillus niger strain HUS7 ASHUS7 , Aspergillus niger strain HG49 ASHG49 and Aspergillus niger strain AN8 ASAN8 showed significant accumulation of citric acid from the submerged basal medium, of which ASHUS 1 significantly a 0.05 recorded the most yield from the production after 9 days of incubation. Mbah, O. G | Okpalla, J. O | Osuala, O. J | Okoye, P. O | Alagbu, P. O "Isolation and Screening for Citric Acid Production by Aspergillus Niger Using Sucrose as a Carbon Source" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46260.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/46260/isolation-and-screening-for-citric-acid-production-by-aspergillus-niger-using-sucrose-as-a-carbon-source/mbah-o-g
International Journal of Engineering Research and DevelopmentIJERD Editor
Electrical, Electronics and Computer Engineering,
Information Engineering and Technology,
Mechanical, Industrial and Manufacturing Engineering,
Automation and Mechatronics Engineering,
Material and Chemical Engineering,
Civil and Architecture Engineering,
Biotechnology and Bio Engineering,
Environmental Engineering,
Petroleum and Mining Engineering,
Marine and Agriculture engineering,
Aerospace Engineering.
Effect of potassium fertilization and organic nutrient (Reef Amirich) in the ...Innspub Net
A field study was conducted at the college of Agriculture, Baghdad University- Jadiriyah to investigate the effect of adding potassium fertilizer and organic nutrient (Reef Amirich) on the population density of two sucking pests of cucumber, cotton whitefly, Bemisia tabaci and onion thrips, Thrips tabaci during the spring season/2016. Results indicated that potassium sulphate (50, 100 and 150 kg/ha) and organic nutrient (0.8 and 1.6ml/l) reduced both the population density of B. tabaci and T. tabaci nymphs depending on the fertilizer level of the user, the treatment 150 kg/ha for the potassium fertilizer and 1.6 ml/L for organic nutrient was the highest among others when minimized density of nymphs by 1.62 nymphs of B. tabaci/disk leaf and 0.38 nymph of T. tabaci / disk leaf. suggesting a possible increase in cucumber resistance to this pest as a result of the treatments, and the result showed the infestation with B. tabaci was higher in the second half of May, the average number of nymph were 5.35 nymph/ disk leaf, while the highest number of T. tabaci was observed in the first half of May, the average number reached to 2.73 nymph/ disk leaf. The result showed the interaction treatment K150A1.6 was significant superior of percentage of K and increased of content in leaves of Fe, Cu and Zn.
The effects of cadmium and cow manure on nodulation and growth attributes of ...Agriculture Journal IJOEAR
Abstract— Different biotic and abiotic factors are involved in the availability of heavy metals in soil including organic matter. In order to study the effects of cow manure on cadmium availability and their interactions on common bean nodulation and growth parameters, an experiment was conducted under greenhouse conditions. The treatments included five cadmium levels (0, 2, 5, 10, 20 mg cadmium per kg soil) and four cow manure levels (0, 15, 30, 60 t ha-1) based on a completely randomized design with three replications. Results indicated that increased soil cadmium concentrations caused higher cadmium uptake by root tissues whereas, Root nodulation and total N content of shoot tissues decreased significantly at all cadmium concentration levels except for 2 mg Kg-1. In addition, the interaction of cow manure and high concentrations of cadmium caused a decrease in nodule number, nodule fresh weights and total N content of shoot in common bean.
Microbiological and Physicochemical Assessment of Poultry Soil Samples in Aku...Agriculture Journal IJOEAR
Abstract— Human activities such as animal production in many parts of the world, still impact negatively on the environment and biodiversity. This study was carried out to assess the microbiological and physicochemical parameters of poultry soil samples. Soil samples were collected from different poultry in Akure metropolis and soil samples from Federal University of Technology, Akure (FUTA) environment as control. Microbiological and physicochemical analyses were carried out using standard methods. The mean total viable bacterial count of poultry soil ranged from 9.02±0.511×105cfu/g in sample site A to 11.2±0.021×105cfu/g in sample site B and there were significant difference (p<0.05) between the bacterial count of poultry soil and control, mean highest fungal load is 6.05±0.301× 103sfu/g. Bacteria isolated were Escherichia coli, Aeromonas hydrophila ,Bacilllus subtilis, Bacillus cereus, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Streptococcus faecium. Escherichia coli had the highest occurrence of 23.08% while Aeromonas hydrophila had the lowest occurrence of 5.13%. Fungi isolated were Aspergillus flavus, Aspergillus niger, Aspergillus fumigates and Saccharomyces species. Aspergillus niger 46.67% had the highest occurrence while Saccharomyces species had the lowest occurrence of 13.33%. Physiochemical analysis showed that poultry soil had mean pH, temperature and Organic Carbon of 7.92±0.34, 34±0.04oC and 14.88±0.56% respectively, Organic Matter is 5.50±0.61%, Nitrogen 1.27±0.03 mg/g, Phosphorus58.92±0.01 mg/g, Potassium55.48±0.23 mg/g, Sodium 41.77±0.91mg/g, Calcium 28.70±0.24mg/g, Magnesium 20.65±0.32 mg/g and Cation exchange capacity146.60±0.11%. The results obtained calls for proper discharge of poultry waste into the environment to prevent transmission of diseases by water borne pathogens.
Nano-coating ,Nano-treatments novel approaches to Extend the post harvest lif...Aisha Kolhar
The seminar that I have shared is Nano coating, nano treatments novel approaches for extending the post harvest life of horticulture produce. An idea how shelf of the fresh produce can be extended.
Uptake and translocation of copper by mycorrhized seedlings Sterculia setiger...researchagriculture
Pot culture experiments were established to determine the effects of arbuscular mycorrhizal fungus (AMF) (Glomus fasciculatum) on tropical gum tree (Sterculia setigera Del.) grown in Copper contaminated soils. AMF and non-AMF inoculated plants were grown in sterilized substrates and subjected to different copper level (0, 200, 400,600, 800 mg kg-1) concentrations. Root and shoot biomasses of inoculated plants were significantly higher than those of non-inoculated. Copper concentrations in roots were significantly higher than those in shoots in both the inoculated and non-inoculated plants, indicating this heavy metal mostly accumulated in the roots of plants. Copper translocation efficiency from root to shoot was lower in mycorrhizal plants than in nonmycorrhizal ones at any Copper addition levels. However, at high soil Copper concentrations, shoot Copper concentration of inoculated plant were significantly reduced by about 50% compared to non-inoculated plants. These results indicated that AMF could promote tropical gum tree growth and decrease the uptake of Cu at higher soil concentrations, thus protecting their hosts from the toxicity of Copper contaminated soils.
Article Citation:
Malick Ndiaye, Cavalli Eric, Diouf Adama, Diop Tahir Abdoulaye.
Uptake and translocation of copper by mycorrhized seedlings Sterculia setigera (Del.) under Copper-contamined soil.
Journal of Research in Agriculture (2012) 1(1): 022-028.
Full Text:
http://www.jagri.info/documents/AG0012.pdf
Uptake and translocation of copper by mycorrhized seedlings Sterculia setige...researchagriculture
Pot culture experiments were established to determine the effects of
arbuscular mycorrhizal fungus (AMF) (
Glomus fasciculatum
) on tropical gum tree
(
Sterculia setigera
Del.) grown in Copper contaminated soils. AMF and non
-
AMF
inoculated plants were grown in sterilized substrates and subjected to different
copper level (0, 200, 400,600, 800 mg kg
-
1
) concentrations. Root and shoot biomasses
of inoculated plants were significantly higher than those of non
-
inoculated. Copper
concentrations in roots were significantly higher than those in shoots in both the
inoculated and non
-
inoculated plants, indicating this heavy metal mostly accumulated
in the roots of plants. Copper translocation efficiency from root to shoot was lower in
mycorrhizal plants than in nonmycorrhizal ones at any Copper addition levels.
However, at high soil Copper concentrations, shoot Copper concentration of
inoculated plant were significantly reduced by about 50% compared to non
-
inoculated plants. These results indicated that AMF could promote tropical gum tree
growth and decrease the uptake of Cu at higher soil concentrations, thus protecting
their hosts from the toxicity of Copper contaminated soils.
An investigation on heavy metal tolerance properties of bacteria isolated fro...AbdullaAlAsif1
The presence of high concentration of toxic heavy metals in industrial waste directly leads to contamination of receiving soil and water bodies and has deleterious impact on both human health and aquatic life. In the present study samples from textile mill effluent from different areas of Jessore city were analyzed for the identification and characterization of bacteria which shows tolerance to Copper, Mercury and Zinc. The bacterial isolates were characterized on the basis of their morphological and physiological studies including size and shape of the organisms, arrangement of the cells, presence or absence of spores, regular or irregular forms, gram reaction, cultural characteristics, IMViC test, H 2S production, nitrate reduction, deep glucose agar test etc. All the bacterial isolates belonged to 3 genera Bacillus, Enterobacter and Pseudomonas. All the gram positive isolates used in our study showed highest level of tolerance to Zn and moderate level of tolerance to Cu while gram negative isolates showed higher tolerance to Zn in comparison with Cu in nutrient broth. But all of the isolates showed almost no tolerance to Hg. So, our bacterial isolates have the probability to use in the treatment of industrial effluent containing heavy metals and thus pollution due to heavy metal can be controlled. The goal of this study was to identify heavy metal tolerant bacteria from the textile effluent. This kind of study is very significant for broader investigation to obtain data about metal tolerant bacteria considering their potential use for bioremediation and about the interactions between metals and bacteria.
ABSTRACT- The development of human civilization throughout history has led to growing disruption of the natural
balance and the occurrence of different types of pollution. Environmental pollution with petroleum and petrochemical
products has been recognized as significant and serious problem. Diesel engine oil, which is one of the major products of
crude oil, constitutes a major source of pollution in our environment. Therefore diesel engine oil can enter into the
environment through wrecks of oil tankers carrying diesel oil, cleaning of diesel tanks by merchants, war ships carrying
diesel oil and motor mechanics. In present study the microorganisms utilising petrol and diesel oil as carbon source were
isolated and investigation of their characteristics towards the production of polyhydroxyalkanoates (PHA), which is now a
days well known as biodegradable polymer.
Key Words- Petrol and Diesel oil contamination, Bioremediation, Biodegradable bacterial polymer, Sudan
Black B staining, 16sr RNA sequencing
Screening of Biosurfactant Bioemulsifier Producing Bacteria from Petroleum Co...ijtsrd
The release of impurities in the environment, containing petroleum and petroleum cogitated products, is engenders of global being taint. It is also a hazardous for human and animal health, since many of these impurities have evidenced to be toxic and oncogenic. Hydrocarbon particles that are secreted into the environment are hard to get rid of, since they change state to surfaces and are captured by surface tension in a water immiscible stage. Bioremediation has tested to be an alternate to lessen the effects caused to impureness of soil and water, applying the metabolic abilities of microorganisms that can apply hydrocarbons as source of carbon and energy, or that can alter them by co metabolism. The proficiency of removal is directly related to the compound’s chemical structure, to its bioavailability deliberation, harmfulness, flexibility and approach and to the physicochemical situation present in the atmosphere. Perwez Qureshi | Dr. Reshma Jaweria "Screening of Biosurfactant/Bioemulsifier Producing Bacteria from Petroleum Contaminated Soil" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46276.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/46276/screening-of-biosurfactantbioemulsifier-producing-bacteria-from-petroleum-contaminated-soil/perwez-qureshi
A study on physiological, anatomical characterization of selected carrot plan...Innspub Net
Carrots were first used for medical purposes and gradually used as food. It is also a good source of magnesium and manganese. Cadmium is a nonessential element that adversely affects plant growth and development. It is considered as one of the significant pollutants due to its high toxicity and more solubility in water. Experiment was conducted in Old Botanical Garden of University of Agriculture Faisalabad to check the response of carrot genotypes grown under Cadmium Chloride stress (0µM, 10µM, 15µM and 20µM). Variety of carrot (gajar) used was Red Gold. Seeds were sown in 12 pots, which were filled with sand, in 2nd week of November. Germination observed after one week of sowing. The experiment was laid out in a completely randomized design with three replicates. Seeds of carrot (red gold) were purchased from Ayyub Agriculture Research Institute (AARI) Faisalabad and sown directly in the plastic pot. 8 seeds per pot were distributed. Hogland solution was also applied to replicates and then I applied Cadmium Chloride stress to the plants to start my experiment. Cadmium stress decreased the uptake and distribution of essential elements in plant. Studies had revealed that heavy metals cause adverse effects on plant growth, which further lead to decrease plant yield and inhibition of enzymatic activities.In the present study, plant growth characteristics, root length and shoot length decreased under Cd stress. In fact, salt stress decreased all attributes in carrot when the concentrations of Cadmium chloride increased higher and higher.
Dissertation ppt biostimulation- a potential practice for wastewater treaat...Sumer Pankaj
Phycoremediation is a green technology that supports the direct use of living green microalgae for in situ, or in place removal, degradation, of contaminants in soils, sludge, sediments, surface water and ground waters by the mechanisms of bio-transformation, bio-accumulation, bio-concentration, bio-sparging.
It can be said by the current study that microalgae has a great potential for the treatment of industrial and municipal wastewaters as compared to the chemical treatments available commercially. Biological systems are much more efficient in cleaning the excess nutrients from the waste water followed by generation of valuable biomass which can be applied in the food, fertilizer, energy production as use of inorganic chemicals like lime and ferrous sulphate generates huge amount of sludge in textile industries, but on the other hand static anaerobic treatment using acclimatized MLSS gives better colour reduction with zero sludge generation. Microalgal cells can be used in free form to treat waste waters containing high C.O.D., high ammonical nitrogen and high TDS. It not only provides a better reduction of chemicals from wastewaters but it also helps to reduce the operational cost of ETP. Microalgaes not only helps to remediate industrial waste waters but also to treat sweage water and to restore natural water bodies like lakes and ponds. As they are active in remediating the chemicals but also it shows an antagonistic effect against some pathogenic germs like total coliforms and fecal coliforms.
These microalgal cells can also be combined with bacterial biomass of activated sludge process to develop an Algal-Bacterial consortium (ALBA) for better enhancement in the reduction of chemicals from the wastewaters as this symbiotic relation of algae and bacteria provides high satiability of the microalgae along with MLSS and faceable in terms of price and economy for instance the bacterial biomass provides carbon dioxide to algal cells for photosynthesis and in return the bacteria acquires oxygen from algae. The harvested biomass from the ETP’s can be used as bio-fertilizers as it consists of appropriate ratio of vital macro and micro nutrients like N,P,K etc. which enhance the growth of plantlets. It can also be used as aqua feeds for shrimps, fishes and molluscs. Furthermore these microlgal cells are non-toxic in the environment as it becomes a part of food chain and do not cause eutrophication. Therefore, micro-algal based treatment is most suitable for the treating the waste waters and restoring the natural water bodies as compared to other chemical treatments.
Isolation and Screening for Citric Acid Production by Aspergillus Niger Using...ijtsrd
The numerous applications of citric acid have increased its demand. It is of great importance to produce citric acid using adequate monosaccharides and dissacharides as they have proven to be easily catabolyzed and decomposed by Fungi when compared to polysaccharides. The superiority of sucrose to other sugars is a result of strong extracellular mycelium bound invertase of A.niger which rapidly hydrolyses sucrose at low pH. This project was undertaken to isolate, screen and produce citric acid in a submerged fermentation by four different strains of Aspergillus niger using sucrose. Aspergillus niger was isolated and characterized from garden soil. Citric acid production from Aspergillus niger in a submerged basal medium was quantitatively determined using titrimetric method. Aspergillus niger strain HUS1 ASHUS1 , Aspergillus niger strain HUS7 ASHUS7 , Aspergillus niger strain HG49 ASHG49 and Aspergillus niger strain AN8 ASAN8 showed significant accumulation of citric acid from the submerged basal medium, of which ASHUS 1 significantly a 0.05 recorded the most yield from the production after 9 days of incubation. Mbah, O. G | Okpalla, J. O | Osuala, O. J | Okoye, P. O | Alagbu, P. O "Isolation and Screening for Citric Acid Production by Aspergillus Niger Using Sucrose as a Carbon Source" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46260.pdf Paper URL: https://www.ijtsrd.com/biological-science/microbiology/46260/isolation-and-screening-for-citric-acid-production-by-aspergillus-niger-using-sucrose-as-a-carbon-source/mbah-o-g
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Effect of potassium fertilization and organic nutrient (Reef Amirich) in the ...Innspub Net
A field study was conducted at the college of Agriculture, Baghdad University- Jadiriyah to investigate the effect of adding potassium fertilizer and organic nutrient (Reef Amirich) on the population density of two sucking pests of cucumber, cotton whitefly, Bemisia tabaci and onion thrips, Thrips tabaci during the spring season/2016. Results indicated that potassium sulphate (50, 100 and 150 kg/ha) and organic nutrient (0.8 and 1.6ml/l) reduced both the population density of B. tabaci and T. tabaci nymphs depending on the fertilizer level of the user, the treatment 150 kg/ha for the potassium fertilizer and 1.6 ml/L for organic nutrient was the highest among others when minimized density of nymphs by 1.62 nymphs of B. tabaci/disk leaf and 0.38 nymph of T. tabaci / disk leaf. suggesting a possible increase in cucumber resistance to this pest as a result of the treatments, and the result showed the infestation with B. tabaci was higher in the second half of May, the average number of nymph were 5.35 nymph/ disk leaf, while the highest number of T. tabaci was observed in the first half of May, the average number reached to 2.73 nymph/ disk leaf. The result showed the interaction treatment K150A1.6 was significant superior of percentage of K and increased of content in leaves of Fe, Cu and Zn.
The effects of cadmium and cow manure on nodulation and growth attributes of ...Agriculture Journal IJOEAR
Abstract— Different biotic and abiotic factors are involved in the availability of heavy metals in soil including organic matter. In order to study the effects of cow manure on cadmium availability and their interactions on common bean nodulation and growth parameters, an experiment was conducted under greenhouse conditions. The treatments included five cadmium levels (0, 2, 5, 10, 20 mg cadmium per kg soil) and four cow manure levels (0, 15, 30, 60 t ha-1) based on a completely randomized design with three replications. Results indicated that increased soil cadmium concentrations caused higher cadmium uptake by root tissues whereas, Root nodulation and total N content of shoot tissues decreased significantly at all cadmium concentration levels except for 2 mg Kg-1. In addition, the interaction of cow manure and high concentrations of cadmium caused a decrease in nodule number, nodule fresh weights and total N content of shoot in common bean.
Microbiological and Physicochemical Assessment of Poultry Soil Samples in Aku...Agriculture Journal IJOEAR
Abstract— Human activities such as animal production in many parts of the world, still impact negatively on the environment and biodiversity. This study was carried out to assess the microbiological and physicochemical parameters of poultry soil samples. Soil samples were collected from different poultry in Akure metropolis and soil samples from Federal University of Technology, Akure (FUTA) environment as control. Microbiological and physicochemical analyses were carried out using standard methods. The mean total viable bacterial count of poultry soil ranged from 9.02±0.511×105cfu/g in sample site A to 11.2±0.021×105cfu/g in sample site B and there were significant difference (p<0.05) between the bacterial count of poultry soil and control, mean highest fungal load is 6.05±0.301× 103sfu/g. Bacteria isolated were Escherichia coli, Aeromonas hydrophila ,Bacilllus subtilis, Bacillus cereus, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Streptococcus faecium. Escherichia coli had the highest occurrence of 23.08% while Aeromonas hydrophila had the lowest occurrence of 5.13%. Fungi isolated were Aspergillus flavus, Aspergillus niger, Aspergillus fumigates and Saccharomyces species. Aspergillus niger 46.67% had the highest occurrence while Saccharomyces species had the lowest occurrence of 13.33%. Physiochemical analysis showed that poultry soil had mean pH, temperature and Organic Carbon of 7.92±0.34, 34±0.04oC and 14.88±0.56% respectively, Organic Matter is 5.50±0.61%, Nitrogen 1.27±0.03 mg/g, Phosphorus58.92±0.01 mg/g, Potassium55.48±0.23 mg/g, Sodium 41.77±0.91mg/g, Calcium 28.70±0.24mg/g, Magnesium 20.65±0.32 mg/g and Cation exchange capacity146.60±0.11%. The results obtained calls for proper discharge of poultry waste into the environment to prevent transmission of diseases by water borne pathogens.
Nano-coating ,Nano-treatments novel approaches to Extend the post harvest lif...Aisha Kolhar
The seminar that I have shared is Nano coating, nano treatments novel approaches for extending the post harvest life of horticulture produce. An idea how shelf of the fresh produce can be extended.
Uptake and translocation of copper by mycorrhized seedlings Sterculia setiger...researchagriculture
Pot culture experiments were established to determine the effects of arbuscular mycorrhizal fungus (AMF) (Glomus fasciculatum) on tropical gum tree (Sterculia setigera Del.) grown in Copper contaminated soils. AMF and non-AMF inoculated plants were grown in sterilized substrates and subjected to different copper level (0, 200, 400,600, 800 mg kg-1) concentrations. Root and shoot biomasses of inoculated plants were significantly higher than those of non-inoculated. Copper concentrations in roots were significantly higher than those in shoots in both the inoculated and non-inoculated plants, indicating this heavy metal mostly accumulated in the roots of plants. Copper translocation efficiency from root to shoot was lower in mycorrhizal plants than in nonmycorrhizal ones at any Copper addition levels. However, at high soil Copper concentrations, shoot Copper concentration of inoculated plant were significantly reduced by about 50% compared to non-inoculated plants. These results indicated that AMF could promote tropical gum tree growth and decrease the uptake of Cu at higher soil concentrations, thus protecting their hosts from the toxicity of Copper contaminated soils.
Article Citation:
Malick Ndiaye, Cavalli Eric, Diouf Adama, Diop Tahir Abdoulaye.
Uptake and translocation of copper by mycorrhized seedlings Sterculia setigera (Del.) under Copper-contamined soil.
Journal of Research in Agriculture (2012) 1(1): 022-028.
Full Text:
http://www.jagri.info/documents/AG0012.pdf
Uptake and translocation of copper by mycorrhized seedlings Sterculia setige...researchagriculture
Pot culture experiments were established to determine the effects of
arbuscular mycorrhizal fungus (AMF) (
Glomus fasciculatum
) on tropical gum tree
(
Sterculia setigera
Del.) grown in Copper contaminated soils. AMF and non
-
AMF
inoculated plants were grown in sterilized substrates and subjected to different
copper level (0, 200, 400,600, 800 mg kg
-
1
) concentrations. Root and shoot biomasses
of inoculated plants were significantly higher than those of non
-
inoculated. Copper
concentrations in roots were significantly higher than those in shoots in both the
inoculated and non
-
inoculated plants, indicating this heavy metal mostly accumulated
in the roots of plants. Copper translocation efficiency from root to shoot was lower in
mycorrhizal plants than in nonmycorrhizal ones at any Copper addition levels.
However, at high soil Copper concentrations, shoot Copper concentration of
inoculated plant were significantly reduced by about 50% compared to non
-
inoculated plants. These results indicated that AMF could promote tropical gum tree
growth and decrease the uptake of Cu at higher soil concentrations, thus protecting
their hosts from the toxicity of Copper contaminated soils.
An investigation on heavy metal tolerance properties of bacteria isolated fro...AbdullaAlAsif1
The presence of high concentration of toxic heavy metals in industrial waste directly leads to contamination of receiving soil and water bodies and has deleterious impact on both human health and aquatic life. In the present study samples from textile mill effluent from different areas of Jessore city were analyzed for the identification and characterization of bacteria which shows tolerance to Copper, Mercury and Zinc. The bacterial isolates were characterized on the basis of their morphological and physiological studies including size and shape of the organisms, arrangement of the cells, presence or absence of spores, regular or irregular forms, gram reaction, cultural characteristics, IMViC test, H 2S production, nitrate reduction, deep glucose agar test etc. All the bacterial isolates belonged to 3 genera Bacillus, Enterobacter and Pseudomonas. All the gram positive isolates used in our study showed highest level of tolerance to Zn and moderate level of tolerance to Cu while gram negative isolates showed higher tolerance to Zn in comparison with Cu in nutrient broth. But all of the isolates showed almost no tolerance to Hg. So, our bacterial isolates have the probability to use in the treatment of industrial effluent containing heavy metals and thus pollution due to heavy metal can be controlled. The goal of this study was to identify heavy metal tolerant bacteria from the textile effluent. This kind of study is very significant for broader investigation to obtain data about metal tolerant bacteria considering their potential use for bioremediation and about the interactions between metals and bacteria.
LABORATORY STUDIES ON THE BIOREMEDIATION OF SOIL CONTAMINATED BY DIESEL IAEME Publication
The most widely used energy and fuel resources are hydrocarbons such as crude oil and petroleum distillates. The accidental discharge of these petroleum products contribute in making hydrocarbons the most common environmental pollutants. Bioremediation helps to destroy or render harmless various contaminants using natural biological activity. The present study utilizes the potential of bioremediation to remediate soil contaminated with diesel. Eight bioreactors were used for the study, out of which four bioreactors were maintained at optimum environmental conditions and the remaining four were kept without any maintenance to serve as control bioreactors. Contaminated soil was prepared by mixing fresh soil and diesel so as to attain 10% TPH concentrations by weight of soil. Each bioreactor was filled with 3 kg of contaminated soil.
Distribution, Biochemical Properties and Genetic Relatedness of Endophytic Ba...AI Publications
Microbe-assisted phytoremediation is a recent application of bioremediation with much prospects. The genetic relatedness of culturable endophytic bacteria of wetland plants growing on a six month-old and twelve month-old petroleum-contaminated sites, and an uncontaminated site in Bayelsa State of the Niger Delta Region, Nigeria were compared. Most of the endophyte species isolated from the roots, stems and leaves were common to all the sites and belong to the phyla Proteobacteria, Bacteroidetes Firmicutes, Actinobacteria, Chloroflexi and Actinomicrobia, with the γ-Proteobacteria dominating. Pseudomonas was the most prevalent species in all three sites, but higher in the petroleum contaminated sites. Biochemical properties (API 20E) of the common isolates; Pseudomonas spp.Chryseobacterium indologenes,Bacillus and Proteusvaried with sites while only Providencia rettgeri peculiar to the petroleum-contaminated sites showed the same properties. 16S rRNA PCR-DNA fragments of forty-five species of the isolates (15 from each site) were characterized using RFLP and MspI restriction enzyme and a genetic distance tree of the restriction patterns drawn. The percentage of similarity in the genetic relatedness of isolates ranged from 11.1 – 100%. The genetic tree analysis of the 45 species of identified bacteria revealed 3 major clusters with 17 DNA fingerprinting patterns. Pseudomonas species of the root and leaves of the six month-old petroleum-contaminated site and uncontaminated site were seen to cluster together irrespective of date of isolation. The endophytes may play a role in the in situ degradation of the petroleum hydrocarbon of the sites.
Effect of Aloe Vera wastes on physico-chemical properties and microbiological...IJEABJ
The aim of the present study was to explore the potential for using aloe vera wastes as amendment for soil to improve its fertility. Soil was exposed to four concentrations of aloin (rich in HAP) for 0, 7, 14 and 28 days. Physico-chemical parameters were analyzed: soil Ph, organic matter (OM), nitrogen, phosphorus, and cation exchange capacity (CEC). The activity of seven enzymes implicated in the C, N and S cycles were measured. Microbial Biomass was determined by the method of substrate induced respiration. BiologEcoplates (Biolog Inc., Hayward, CA) were used to estimate soil microbial functional diversity. Our findings suggested a decrease on phosphorus and nitrogen content and an increase on CEC after aloin addition. Also, a decrease on microbial biomass and enzymes activities was observed, except for FDA. Ecoplates results demonstrate a decrease on microbial activities depending on the incubation time. Moreover, our results indicated that bacterial communities of the tested soils have more affinity to consume substrates as Amino acids and polymers. Our results should be carefully considered in view of the agriculture waists reuse for a sustainable agriculture
Evaluation of the Effectiveness of Fungi (Candida Tropicalis and Aspergillus ...IJEABJ
Used engine oil is a petroleum or synthetic oil that has been used and as a result of such use, is contaminated by physical and chemical pollutants. These pollutants are harmful to humans, animals and plants following exposure. Evaluation of the effectiveness of fungi in bioremediation of used engine oil (UEO) contaminated soil was investigated. Fungi were isolated from soil samples obtained from automobile workshops in Mgbuka-Nkpor, Nigeria. The isolates were screened for UEO biodegradation potentials in mineral salt broth. They were identified using the cultural and microscopic characteristics and confirmed using the 18SrRNA gene sequence. The effectiveness of the isolates in bioremediation of UEO contaminated soil was also investigated using bioaugmentation technique. A total of 8 fungal isolates were obtained from this study. Two that showed the highest extent of biodegradation of UEO in the screen flasks were identified and confirmed as Candida tropicalis and Aspergillus clavatus. At the end of the experimental period, oil contaminated soil inoculated with the mixed culture of the isolates (C. tropicalis and A. clavatus) showed the highest reduction in concentration of UEO (95.42%). Higher biodegradation rate and shorter half-life of total petroleum hydrocarbon (TPH) was observed in soil microcosm containing the isolates, when compared to the uninoculated control. Therefore fungi such as C. tropicalis and A. clavatus isolated from automobile workshops can facilitate the bioremediation of UEO contaminated soil.
The present study intended to compare the efficiency of the wetland plants Phragmites australis (cav.)
Trin. ex Steudel and Cyperus papyrus L. for the accumulation of heavy metals. Maximum accumulation
of the heavy metals Pb (132.5-175 μg/g DW), Zn (97.5-100 μg/g DW) and Cr (80.5-90 μg/g DW) was
recorded in roots of the two wetland plants C. papyrus and P. australis, respectively. Cadmium showed
the lowest metal accumulation in the tissues of both plants (0.55-5.5 μg/g DW). C. papyrus roots
accumulated higher levels of most metals than those of P. australis. Heavy metals were shown to be not
only accumulated in roots of both plants but also were translocated to the shoots and accumulated in the
harvestable plant parts. The results showed variation in the levels of the heavy metals accumulated in the
different parts of C. papyrus and P. australis, in spite of being planted at the same site and subjected to
the same conditions. The potential use of Phragmites australis and Cyperus papyrus in phytoremediation
is also discussed.
DOI:10.21276/ijlssr.2016.2.4.19
ABSTRACT- Halophiles, the most predominant organisms found in the mangrove forest, include halophilic bacteria in different environment such as salt lakes, saline soils and salted food. The majo rviatyri eodf hgaelnoeprhai loicf pmriocproerotrigesa nwishmicsh s tfuadciielidta steo iftasr upsreo dwuicteh ccoommpmoeurncdias l waiitmh sg. rIena tt hpiost esntutidayl ionf inSduunsdterirabla pnr osoceils,s faoncdu st hheays hbaeveen pmhaydsieo loong itchael iasnodla wtieorne oufs ehda lfooprh pilhiyc soiorgcahnemismicsa la anndd t mheiicrr ochbaioralocgteicriasla atinoanl.y sSiosi. lQs uwaelritea tciovlel escctreede nfirnogms foofu trh ed iifsfoelraetnets pwlaecree sd oofn eS uanndd etrhbraene aCmhaornagc tethriesmat iowna so f stehlee citseodl ahteasv iwnegr em doodneer aatneldy bgaoseodd ognr o1w6Sth rRwNhiAch g ewnaes sefuqrutehnecr inogp tpimhyisloegde nine tidci ftfreeree nwt egrer ocwotnhs trmucetdeida.. aTsh we eisllo laast evsa rsihoouws eedx tmrauceltlilpullea rh eenazvyym mee ptarol dtoulcetriaonncse t haenrde bayn teixbpiolotirci nrge stihsetairn cues.a gTeh efo or rvgaarnioisums sb iwoeterceh fnuortlohgeric taels pteudr pfoosr eEs.P S Key-words- Sundarban, Halophiles, EPS, NaCl
Metabolomics Analysis on Antifungal Activities Produced by Penicillium oxalic...Agriculture Journal IJOEAR
—In-vitro antagonist tests such as disc diffusion and minimum inhibition concentration (MIC) were conducted against C. gloeosporioides. 1 H-NMR coupled with multivariate statistical analysis was carried out to identify possible compounds produced. Glucose crude extract exhibited the highest percent inhibition of radial growth (PIRG) with 75% and the lowest MIC value with 78 µg mL-1. For metabolomics, different metabolites produced were clustered according to the carbon sources used and gave a representative impression of the metabolites produced by P. oxalicum T3.3. The study has shown the potential of using a combination of 1 H-NMR spectroscopy and multivariate statistical analysis and their correlation with MIC in differentiating the effect of carbon sources used based on the identification of possible metabolites contributing to their differences. Findings from this work may potentially provide the basis for further studies on both antimicrobial activities against plant pathogen and elucidation of the metabolite compounds produced by P. oxalicum T3.3.
Soil is a mixture of broken rocks and mineral which contain living organisms, in which microorganisms can transfer either by erosion or wind dispersal to other living things when come in close contact which can be as a source of contact to pathogenic microbes and can ponderous to health hazard of the community. This study investigates the microbial analysis and also to check the influence of soil with fallen rotten cocoa against soil without rotten cocoa on soil microbes in Ikeji-Arakeji, Osun state, Nigeria. Total bacterial and fungal counts were determined using pour plating method. Total bacterial count was higher from soil with fallen rotten cocoa pod 92 ×105 cfu/ml to soil without fallen rotten cocoa which is 86 ×105 cfu/ml and Total fungal count ranges was higher from soil with fallen rotten cocoa pod is 44 ×105 cfu/ml to soil without rotten cocoa pod which is 33 ×105 cfu/ml. The isolation and enumeration of microbial population was carried out using standard culture-based methods. Bacteria isolates such as Proteus vulgaris, Escherichia coli, Pseudomonas sp, Enterobacter aerogene., Bacillus spp, Staphylococcus spp, Micrococcus sp, Erysipelothrix spp and the fungi isolate include Aspergillus niger, Aspergillus flavus and Rhizopus stolonifer. All the microbes were present for the two samples except Micrococcus sp and Enterobacter aerogenes. The bacteria count were more alpine and higher in bacterial count than the fungal count. The presence of these microorganisms indicates a suitable soil for general plantation by the microbial degradative activities.
PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANTS PRODUCED BY Pseudomonas aer...UniversitasGadjahMada
The biosurfactants are used by several industrial sectors such as petroleum, agriculture, food production, chemistry, cosmetics, and pharmaceuticals. Because of their hydrophobic and hydrophilic moieties, they have potency to reduce surface tension, interfacial tension between water-hydrocarbon systems, and low micelle concentration. Their characteristics strongly depend on the producer strain as well as on the medium composition, such as carbon and nitrogen sources. This study was conducted to investigate the influence of different sources of carbon (n-hexadecane, glycerol and glucose) and nitrogen (urea, NH4Cl and NaNO3 ) for the production of biosurfactants by a new strain of Pseudomonas aeruginosa B031 isolated from a rhizosphere of Paraserianthes falcataria L. Nielsen, a hardwood plant species at a phytoremediation field. The biosurfactant characteristics of the strain were evaluated, particularly its surface-active properties and potential to remove hydrocarbon. Glycerol was found to be the optimum carbon source, with rhamnose concentration, emulsification index, and critical micelle concentration (CMC) of 718 mg/L, 37%, and 35 mN/m, respectively. Sodium nitrate (NaNO3 ) was observed as the optimum nitrogen source, with rhamnose concentration, emulsification index, and CMC of 290 mg/L, 30%, and 24 mN/m, respectively. These biosurfactants efficiently reduced surface tension of culture broth from 42 mN/m to 31 mN/m for the glycerol treatment and from 37 mN/m to 24 mN/m for the sodium nitrate treatment. The crude biosurfactants from the glycerol and sodium nitrate treatments also removed 87.5% and 84%, respectively, of crude oil from sand. These rates were higher than those of the chemical surfactants (SDS and Triton X-100). These findings indicate that the biosurfactants produced by the strain from both glycerol and NaNO3 treatments can efficiently decrease the interfacial tension of culture broth dilution and have a high emulsion index, thus hold promise in hydrocarbon bioremediation application.
Similar to Assessment and characterization of rhizo bacteria in petroleum–polluted soil in south–east, nigeria (20)
Assessment and characterization of rhizo bacteria in petroleum–polluted soil in south–east, nigeria
1. Journal of Natural Sciences Research www.iiste.org
ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online)
Vol.4, No.21, 2014
25
Assessment and Characterization of Rhizo-Bacteria in Petroleum–
Polluted Soil in South–East, Nigeria
Kelechi Ukaegbu-Obi.1*
Yusuf Omeh2
1.Department of Microbiology, Michael Okpara University of Agriculture,Umudike, P.M.B.7267, Abia State
Nigeria
2.Department of Biochemistry, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
* E-mail of the corresponding author: kelechi.ukaegbuobi@yahoo.com
Abstract
A rhizoremediation study was carried out on petroleum-polluted soil in Akiri, Imo State, Nigeria.
The assessment and characterization of bacteria capable of degrading hydrocarbons were isolated from the
rhizosphere of Mimosa pudica, Alchornia chordifolia, Chromolaena ordorata, Chloris pilosa and Sida acuta.
The isolates identified include Acinetobacter, Bacillus, Micrococcus, and Pseudomonas spp. All the isolates
grew on petroleum hydrocarbon at different growth rates. Statistical analysis showed no significant difference
(P>0.05) between the rhizosphere and non-rhizosphere of total culturable heterotrophic and hydrocarbon-
utilizing bacterial counts in both petroleum-polluted and unpolluted (control) soils. All the plants exhibited
positive rhizosphere effects on the rhizo-bacteria. Based on these results, the rhizo-bacteria isolated can serve as
seeds for bioaugmentation during remediation of crude oil polluted soil environment. The plants may be
employed in rhizoremediation of oil polluted soil.
Keywords: Characterization, Rhizoremediation, Bacteria, Rhizosphere effect, Petroleum-polluted soil
1. Introduction
The accumulation of petroleum hydrocarbons in the environment can cause serious problems, affecting
negatively the stability of many ecosystems and can also cause difficulties for animals and human health
(Chekroun et al., 2014). Chemical contaminants present in the aquatic ecosystem may be immobilized and
accumulated in sediments or may be subject to transformation and activation processes (Martínez-Jerónimo et al.,
2008).
Remediation of soils containing organic pollutants can be enhanced by plants by various processes (Cunningham
et al., 1996). In-situ phytoremediation strategy exploits natural or genetically engineered plant species to
accumulate toxic substances (heavy metals, radioactive compounds, organic pollutants) directly from the soil.
Partial or complete degradation of organic substances have been demonstrated in some cases (White, 2001).
Most plants have symbiotic relationships with soil microorganisms. The area around plant roots, otherwise called
the rhizosphere contains higher populations, greater diversities and activities of microorganisms (Brimecombe et
al. 2007) than soil with no plants. Rhizosphere microorganisms are especially critical for plant colonization of
unfavourable soils, since they can alleviate biotic and abiotic stress of plants. This has caused the emergence of a
green technology which employs the symbiotic relationship between plants and their rhizo-microorganisms in
the breakdown of contaminants to clean up the environment. This technique is referred to as rhizoremediation
(Kuiper et al., 2004). A plant can be considered to be a solar-driven biological pump and treatment system,
attracting water with its root system, accumulating water-soluble pollutant in the rhizosphere and concluding
with the degradation or translocation of pollutants (Liste and Alexander, 2000). In some cases, rhizosphere
microbes are even the main contributors to the degradation process. Plants release exudates into the soil
ecosystem that increases the microbial activity and aid the degradation of xenobiotic substances. The soluble
root exudates include enzymes, amino acids, sugars and low molecular weight carbohydrates (Burken and
Schnoor, 1996).
The plant rhizosphere is recognized as a niche rich in growth substrates in comparison with the surrounding bulk
soil (Dunfield and Germida, 2001). Rhizospheres are dynamic microenvironments in which microbial
communities have access to an elevated supply of carbon, oxygen and energy rich materials from plant roots
(Clegg and Murray, 2002). Rhizospheres are also stable physically, avoiding the potentially adverse effects of
naturally occurring disturbances on microbial community composition or activities (Piceno et al., 2000). This
stimulatory rhizosphere effect has been recognized for many years and was described for the first time by Hiltner
in 1904 (Kuiper et al., 2004). In rhizoremediation, plant roots sustain the degrading microflora by supplying
them with nutrients other than pollutants, and also help in spreading the degrading microorganisms to new sites
in the soil.
2. Journal of Natural Sciences Research www.iiste.org
ISSN 2224-3186 (Paper) ISSN 2225-0921 (Online)
Vol.4, No.21, 2014
26
The aims of this research, therefore, were to enumerate, isolate, and characterize the hydrocarbon-utilizing
bacterial genera associated with the rhizosphere of these plants: Mimosa pudica, Alchornia chordifolia,
Chromolaena ordorata, Chloris pilosa and Sida acuta found in petroleum-polluted areas of Akiri in Oguta in
Imo State, determining the rhizosphere effect ratios of these plants on their rhizobacteria as well as assessing the
potentials of the associated bacteria isolated to utilize crude oil.
2. Materials and Methods
2.1 Site description and Sample Collection
Samples were obtained from a location in Akiri in Imo State where there has been oil spill. This site could be
described as a disturbed ecosystem with scanty plants growing at this location. Soil samples were collected from
plants’ root zones and thirty centimeters away from root zone. Pristine soil samples were collected from plants’
root zones and thirty centimeters away from the root zone in the same area where there has been no known spill
to serve as control. These were placed in separately marked sterile plastic bags and transported in an ice chest to
the laboratory for analysis, after which the plants were taken to a plant taxonomist for identification.
2.2 Sample Processing
The roots were freed from adhering soil which is assumed to be the rhizosphere soil. Ten grams (10) from each
of the samples of rhizosphere and non-rhizosphere of crude oil polluted and unpolluted soils were suspended
ninety millilitres (90ml) of distilled water. The content of the flasks were serially diluted. From each dilution of
10-3
to 10-6
, aliquots (0.1ml) were plated on sterile Nutrient agar (NA) and Mineral Salt agar amended with an
antifungal agent, Natamycin of quantity 21.6mg/L in duplicates.
2.3 Enumeration of bacterial counts:
The total heterotrophic bacterial count was carried out using the spread plate method on Nutrient agar. The plates
were incubated at 35o
C for 24 to 48 hours.
The vapour phase transfer method was adopted for the enumeration of hydrocarbon-utilizing bacteria on the Mineral
Salt agar with the following composition: NaCl 10.0g, MgSO4 0.42g, KCl 0.29g, KH2PO4 0.83g, NaPO4 1.25g,
NANO3 0.42g, agar 15g and distilled water 1000ml as modified by Okpokwasili and Okorie (1988).
2.4 Characterization and Identification of Isolates
The bacterial isolates were examined for colonial morphology, cell micro-morphology and biochemical
characteristics. Tests employed included:
Gram staining, Motility test, Catalase test, Citrate Utilization test, Indole test, Hydrogen Sulphide Production test,
Methyl Red -Voges Proskauer test, Oxidase test, Sugar Fermentation test. Confirmatory identities of the bacteria
were made using the Bergey’s Manual of Determinative Bacteriology (Holts, 1993).
2.5 Biodegradation Test
The bacteria isolated were tested for their ability to degrade crude oil using the turbidity method as described by
Ibrahim et al. (2008). Nutrient broth was used to culture the bacterial isolates and incubated for 24 hours at
28+20
C. 0.1ml of the young culture in nutrient broth grown was inoculated into each test tube containing 9.9ml
of sterile mineral salt broth and 0.1ml of crude oil. A control test tube containing 9.9ml of sterile mineral salt
broth with 0.1ml of crude oil remained un-inoculated. Turbidity of the tubes was used to determine the growth
of the inocula, as compared with the un-inoculated control tube.
2.6 Physicochemical analysis of soil samples
The pH and temperature of the soil samples were determined using Suntex pH MV Temp TS2 metre (MODEL
SP701) in 1:1 soil solution in distilled water in accordance with the manufacturer’s directions.
2.7 Statistical Analysis
The SPSS software of statistical analysis was used to analyze the results of this work. The statistical tools – One-
way Analysis of Variance (ANOVA) was used to analyze the data obtained from all the plants in the various
locations while Independent Student’s t-test was used to analyze the polluted and unpolluted soil sample of each
plant.
3. Results
3.1 Bacteriological analyses
The result of total heterotrophic counts (Figure 1) ranged from 6.91 x 105
cfu/g to 9.08 x 105
cfu/g for the
polluted rhizosphere. In the unpolluted rhizosphere, it ranged from 1.92 x 105
cfu/g to 2.61 x 105
cfu/g. The
counts from the non-rhizosphere ranged from 2.39x105
cfu/g to 3.02x105
cfu/g for the polluted soil and
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3.02x105
cfu/g to 3.25x105
cfu/g for the unpolluted soil.
The result of total heterotrophic counts (Figure 1) ranged from 6.91 x 105
cfu/g to 9.08 x 105
cfu/g for the
polluted rhizosphere. In the unpolluted rhizosphere, it ranged from 1.92 x 105
cfu/g to 2.61 x 105
cfu/g. The
counts from the non-rhizosphere ranged from 2.39x105
cfu/g to 3.02x105
cfu/g for the polluted soil and
3.02x105
cfu/g to 3.25x105
cfu/g for the unpolluted soil.
Figure 1 Distribution of heterotrophic bacteria in polluted and unpolluted
rhizosphere and non- rhizosphere soil in Akiri.
Figure 2: Distribution of Hydrocarbon-degrading bacterial in polluted and unpolluted rhizosphere and non-
rhizosphere soil in Akiri.
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Figure 3. Rhizosphere effect ratio on Culturable heterotrophic counts for polluted and unpolluted soil samples
Figure 4 Rhizosphere effect ratio on Hydrocarbon-degrading bacterial counts for polluted and unpolluted soil
samples
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Figure 5. The percentage of hydrocarbon-utilizers in polluted and unpolluted rhizosphere and non-rhizosphere
soil samples.
3.2 Physicochemical parameters.
3.2.1 Temperature Record
The temperature values recorded during this study were observed to be more supportive to mesophiIic
microorganisms. The values ranged from 250
C to 270
C (Table 1).
3.2.2 pH Reading
The pH values obtained during this study period for the polluted rhizosphere and non-rhizosphere were slightly
acidic while the pH values for the unpolluted rhizosphere and non-rhizosphere soils were almost neutral (Table
2).
Table 1. Temperature Values of Polluted and Unpolluted Rhizosphere and Non-Rhizosphere Soils.
Plant Species Temperature (O
C)
Rhizosphere Bulk
polluted unpolluted polluted unpolluted
Mimosa pudica 25.0 26.3 25.4 26.5
Alchornia cordifolia 25.1 26.5 25.4 26.7
Chromolaena odorata 25.0 26.8 25.4 26.1
Chloris pilosa 25.1 26.5 25.5 26.4
Sida acuta 25.3 26.4 25.5 26.7
Table 2. pH Values of Polluted and Unpolluted Rhizosphere and Non-Rhizosphere Soils.
Plant Species pH
Rhizosphere Bulk
Polluted unpolluted polluted unpolluted
Mimosa pudica 6.40 7.10 5.99 7.00
Alchornia cordifolia 6.25 7.02 6.10 7.03
Chromolaena odorata 5.84 6.95 5.82 6.99
Chloris pilosa 5.67 7.03 5.54 6.97
Sida acuta 5.50 6.92 5.80 7.02
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Table 3: Screen Test for the Utilization of Petroleum Hydrocarbon by the Bacterial Isolates
Isolate code Growth in medium Bacterial isolate
DSA 1A ++ Bacillus sp.
DRA 4B ++ Bacillus sp.
DRA 5A +++ Pseudomonas sp.
CSA 3A + Acinetobacter sp.
CRA 2B + Micrococcus sp.
Key: Heavy growth = +++ Moderate Growth = ++ Scanty Growth = +
4 Discussion
Soils containing diverse organic pollutants, including organic solvents, pesticides, explosives and petroleum can
be remediated by the use of plants and their interaction with root microorganisms which provide nutrients in the
rhizosphere leading to an increased microbial activity, survival of plants and degradation of toxic pollutants
(Mirsal, 2004).
The bacterial counts of polluted and unpolluted rhizosphere and non- rhizosphere are shown in Figures 1 and 2.
It was observed that the total heterotrophic bacterial counts were higher in the polluted rhizosphere than in the
polluted non-rhizosphere of all the plants. This was the same for hydrocarbon-utilizing bacteria; the counts were
also higher in the polluted rhizosphere than the polluted non-rhizosphere. Lynch (1990) stated that on a per gram
basis, rhizosphere soil has 10–100 times more microbes than unvegetated soil. This expresses the rhizosphere
effect of the plants on the bacteria.
The low counts of heterotrophic bacteria (Figure 1) recorded in this study for most petroleum-polluted soils
compared to that of unpolluted soils agreed with the previous report by Jensen (1975), Umanu et al. (2013) and
Ukaegbu-Obi and Mbakwem-Aniebo, (2014) which could be attributed to the toxic effect of petroleum-pollution.
Microbial community structure has been recommended as a biological indicator of heavy metal stress (Yao et al.,
2003).
In Figure 2, hydrocarbon utilizing bacteria were found to be higher in petroleum-polluted soils than the
unpolluted soils. This finding collaborates with the reports of Hubert et al (1999), Michalcewicz (1995),
Ukaegbu-Obi and Mbakwem-Aniebo (2014). These higher populations of petroleum degraders recorded could
be due to the stimulatory effect of additional carbon and energy source in the form of pollutant. The
contamination of crude oil results in an immediate change in the bacterial community structure, an increasing
abundance of hydrocarbon-degrading microorganisms and a rapid rate of oil degradation, which suggests the
presence of a pre-adapted, oil-degrading microbial community and sufficient supply of nutrients (Coulon et al.,
2006; Hamamura et al., 2006; Tang et al., 2010 ).
The values of rhizosphere effect ratios (RER) of the Culturable heterotrophic and hydrocarbon-utilizing bacteria
of the polluted samples were higher than in the unpolluted sample (Figures 3 and 4). It has been speculated that
when a chemical stress is present in the soil, plants respond either by increasing or changing exudation to the
rhizosphere, which modifies the composition or activity of the rhizosphere microflora. Soderberg et al. (2004)
observed that root exudates support growth of the microbial community in the rhizosphere and will result not
only in an increased population density, but also in a community structure distinct from that in the bulk soil.
The pH values were more acidic in the polluted rhizosphere and non-rhizosphere than the unpolluted rhizosphere
and non-rhizosphere. This may be as a result of the time interval between time of spill (April, 2009) and time
sampled (June, 2009). This shows that the acidic components of the crude oil were still present in the soil. The
pH values for polluted rhizosphere and non-rhizosphere ranged from pH 5.50 to pH 6.40 and pH 5.54 to pH 6.10
respectively. The unpolluted rhizosphere and non-rhizosphere pH values recorded were ranged from pH 6.92 to
pH 7.10 and pH 6.97 to pH 7.03 respectively (Table 2). The result in this study agrees with the findings of
Dibble and Bartha (1979) who observed an optimal pH of 7.8, in the range of 5.0 to 7.8, for the mineralization of
oily sludge in soil. The pH values recorded in this study, therefore, would support biodegradation of
hydrocarbons.
The constant warm temperatures of the tropics favor plant growth during the entire year in contrast to temperate
zones. Microbial activity and thus bioremediation is greatly enhanced due to more intense temperatures, higher
humidity and solar radiation (Prado- Jatar et al., 1993). Maximum degradation rates of organics have been found
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to occur at 30 and 400
C, that is temperatures in tropical savannah soils (Rivera-Cruz et al., 2004).
Results of the distribution of hydrocarbon-utilizing bacteria in the polluted rhizosphere and non-rhizosphere soil
of the plants show that the polluted rhizosphere soil of the plants stimulated the development of higher counts
(cfu/g soil) of these organisms as compared to the non-rhizosphere soil (Figure 5). The percentage of
hydrocarbon-utilizers was also higher in the rhizosphere soil, than in the non-rhizosphere soil. Diab (2008) made
similar observation; he reported that, the percentage of the oil degraders were higher in the rhizosphere soil than
in the non-rhizosphere. The above result confirm the ability of plant roots in symbiotic relationship with
rhizomicrobes to neutralize or to remove the toxic effects of the oil pollutants, probably through the plant
exudates, nutrients, oxygen supply and other materials to the microbes.
Murotova et al. (2003) explained that the success of phytoremediation of hydrocarbon contaminated soil is
connected with the plant’s capacity to enhance microbial activity in the rhizosphere. The efficiency of this
process is often connected with higher number of degrader microorganisms and their degradative activities in the
rhizosphere of plants. The unpolluted rhizosphere results of hydrocarbon-utilizers percentage in this study
showed higher values also than unpolluted non-rhizosphere. The values of the hydrocarbon-utilizers percentage
in polluted rhizosphere and non-rhizosphere are within the reported frequencies for soil bacteria as reviewed by
Leahy and Colwell (1990). The fraction of the total heterotrophic community represented by the hydrocarbon-
utilizing bacteria and fungi is highly variable. This suggests that the unpolluted sites (which served as control)
which had no known history of pollution as at the time of sampling may have had a prior exposure to
hydrocarbons which could be either from anthropogenic sources (especially since these areas are oil producing
areas and experience steady petroleum exploration) or natural sources such as plant derived hydrocarbons. A
number of reports, reviewed by Atlas, (1981) and Leahy and Colwell,(1990) have shown that the numbers of
hydrocarbon-utilizing microorganisms and their proportion in the heterotrophic community increased upon
exposure to petroleum or other hydrocarbon pollutants and that the levels of hydrocarbon-utilizing
microorganisms generally reflect the degree of contamination of the ecosystem.
It is generally accepted that bulk soil and rhizosphere microbial community structure is determined by the local
native microbial community, impacted by the soil effects and vegetation (Juhanson et al., 2008). The
composition of the microbial population in the rhizosphere depends on the composition of the exudates as well
as on the plant species, root type, plant age, soil type and history of soil (Kuiper et al., 2004). The bacterial
isolates obtained from the different rhizosphere and non-rhizosphere of polluted and unpolluted soils in this
study were identified to be of the following genera: Bacillus, Acinetobacter, Alcaligenes, Arthrobacter,
Corynebacterium, Flavobacterium, Micrococcus, Pseudomonas and Serratia (Table 4). This agrees with the
findings of Tesar et al. (2002) who reported that a broad phylogenetic range of bacteria including species/strains
of Achromobacter, Acidovorax, Alcaligenes, Arthrobacter, Bacillus, Corynebacterium, Flavobacterium,
Micrococcus, Mycobacterium, Norcadia, Pseudomonas, Rhodococcus, Sphinogomonas and Xanthomonas have
been identified in the breakdown of hydrocarbons. The isolates were predominantly rod shaped bacteria (88.89%
of the isolates were rods and 11.11% cocci).
The bacterial isolates utilized crude oil at different rates with Pseudomonas sp. having the highest growth rate
followed by Bacillus sp. that had moderate growth rate and Acinetobacter sp. and Micrococcus sp. having scanty
growth rates (Table 3)
The biodegradation of petroleum and other hydrocarbons in the environment is a complex process, whose
quantitative and qualitative aspects depend on the nature and amount of the oil or hydrocarbons present, the
ambient and seasonal environmental conditions and the composition of autochthonous microbial community and
their adaptive response to the presence of hydrocarbons.
The results of this study indicate that hydrocarbon-utilizing bacteria can be found in the rhizosphere Mimosa
pudica, Alchorinia cordifolia, Chromolaena ordorata, Chloris pilosa and Sida acuta. These bacteria are not
restricted to petroleum contaminated soil though. Qualitative differences in root exudates among these plants
also induced the increase of bacterial population in the rhizosphere. Thus, rhizoremediation is a promising
technology for the clean-up of petroleum-contaminated soils, especially in the tropics where climatic conditions
favour plant growth and microbial activity and where financial resources can be limited.
5. Conclusion
This symbiotic technology should therefore be adopted as it is environmentally friendly and aesthetically
pleasing, less expensive, self-sustaining and can be used for a wide variety of pollutants. Furthermore,
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rhizoremediation generates less secondary waste and fewer air emissions. This is a welcome development
especially now that our environment suffers a lot from pollution.
References
Atlas, R.M. (1981). Microbial degradation of petroleum hydrocarbon: an environmental perspective. Microbial.
45: 180 – 209.
Brimecombe, M.J., De Leij, F. A. A. M., Lynch, J.M. (2007). Rhizodeposition and microbial populations. In: R
Pinton, Z Varanini, P Nannipieri (eds) The rhizosphere: biochemistry and organic substances at the soil-plant
interface. CRC Press, Taylor & Francis Group, Boca Raton, London,New York, pp 73–109.
Burken, J.G. and J.D.L. Schnoor. (1996). Phytoremedation: Plant Uptake of Atrazine and Role of Root Exudates.
J. Environ. Eng. 122: 958–963.
Chekroun, K.B Sánchez, E. and Baghour, M. (2014). The role of algae in bioremediation of organic pollutants.
Int. Res. J. Public Environ. Health 1 (2): 19-32
Coulon, F., McKew, B. A., Osborn, A. M., McGenity, T. J., andTimmis, K. N. (2006). Effects of temperature
and biostimulation on oildegradingmicrobial communities in temperate estuarine waters, Environ. Microbiol. 9:
177–186.
Cunningham, S.D., T.A. Anderson, A.P. Schwab, and F.C. Hsu. (1996). Phytoremediation Of Soils
Contaminated With Organic Pollutants. Adv. Argon. 56:55-114.
Diab, E.A. (2008). Phytoremediation in oil contaminated desert soil using rhizosphere effect. Global Environ
Res. 2 (2): 66 – 73.
Dunfield, K.E. and J.J. Germida. 2001. Diversity of bacterial communities in the rhizosphere and root interior
of field grown genetically modified Brassica napus. FEMS Microb. Ecol. 38. 1-9.
Erickson, L.E. (1997). An Overview of Research on the Beneficial Effects of Vegetation in Contaminated Soil.
Ann. N.Y Acad. Sci. 829: 30–35.
Hamamura, N., Olson, S. H., Ward, D. M., and Inskeep, W.P. (2006). Microbial Population Dynamics
Associated with Crude-Oil Biodegradation in Diverse Soils. Appl. Environ. Microbiol. 72: 6316–6324.
Juhanson, J., J. Truu, E. Heinaru and A. Heinaru. (2008). Temporal dynamics of microbial community in soil
during phytoremediation field experiments. JEELM. 15 (4): 213– 220.
Kuiper, I., Lagendijk, E.L. Bloemberg, G.V. and Lugtenberg, B.J.J. (2004). Rhizoremediation: A beneficial
plant-microbe interaction. Mol. Plant-Microbe Interact. 17(1):6-15.
Leahy, J. G. and Colwell, R. R. (1990). Microbial degradation of hydrocarbons in the environment. Microbial.
Rev. 54: 305 – 315.
Liste, H.H. and M. Alexander. (2000). Plant Promoted Pyrene Degradation in Soil.Chemosphere. 40:7-10.
Lynch, J.M. (1990). The Rhizosphere. Wiley, New York.
Martínez-Jerónimo F, Cruz-Cisneros JL, García-Hernández L (2008). A comparison of the response of
Simocephalus mixtus (Cladocera) and Daphnia magna to contaminated freshwater sediments. Ecotoxicol.
Environ. Saf. 71:26–31.
Mirsal, I. (2004) Soil pollution: origin, monitoring and remediation. Springer, New York
Murotova, A., Hubner, T., Narula., N. Wand, H., Turkovskaya, O., Kuschk, P., John, R. and Merbach, W.
(2003). Rhizosphere microflora of plants used for the phytoremediation of bitumen-contaminated soil.
Microbiol. Res. 158: 151–161.
Okpokwasili, G.C. and Okorie, B.B. (1988). Biodeterioration potential of microorganisms isolated from car
engine lubricating oil. Tribology International. 21:215–217.
Soderberg, K.H., A. Probanza, A. Jumpponen and E. Baath. 2004. The microbial community in the
rhizosphere determined by community level physiological profiles (CLPP) and direct soil and cfu –
PLFA techniques. J. Appl. Soil Ecol. 25: 135 – 145.
Tang, J. C.,Wang, R. G., Niu, X. W., Wang, M., Chu, H. R. and Zhou, Q. X (2010). Characterisation of the
rhizoremediation of petroleum-contaminated soil: effect of different influencing factors. Biogeosciences. 7:
3961–3969.
Umanu, G., Akpe, A.R. and Omoikhudu, A. R. (2013). Oil Degradation Assessment of Bacteria Isolated from
Used Motor Oil Contaminated Soils in Ota, Nigeria. I.J.A.B.R. 3(4): 506-513. ISSN 2250 – 3579.
Ukaegbu-Obi, K. M. and Mbakwem-Aniebo, C.C. (2014). Bioremediation Potentials of Bacteria Isolated from
Rhizosphere of Some Plants of Oil Contaminated Soil of Niger Delta. Journal of Applied & Environmental
Microbiology. 2 (4): 194-197. DOI:10.12691/jaem-2-4-13
White, P. (2001). Phytoremediation Assisted by Microorganisms. Trends Plant Sci. 6: 502.
Yao, H., Xu, J. and Huang, C. (2003). Substrate utilization pattern, biomass and activity of microbial
communities in a sequence of heavy metal polluted paddy soil. Geoderma. 190: 1-10
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Figure 1. Architecture of WOZIP
Table 1. Datasheet of Mock-up Test
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