1. Bioethanol From Sugarcane Waste (Bagasse) Using
Bacterium Clostridium Thermocellum
Ari Permana Putra1), Delmar Zakaria Firdaus1), Ginanjar Ummi Pratiwi1)
Departement of Agroindustrial Technology, Faculty of Agricultural Technology and Engineering, Bogor Agricultural University
Bogor, West Java, Indonesia
The 1st Annual International Scholars Conference in Taiwan 2013
Asia University, Taichung, Taiwan
April 27th – 29th 2013

2. Thermal Inversion
Smog surrounds the Angel Monument in Mexico
City, Mexico, during a thermal inversion. Air
pollution increases dramatically when a mass of
cold air is trapped below a mass of warmer air.
The absence of air circulation prevents
pollution near Earth’s surface from escaping.
Wesley Bocxe/Photo Researchers, Inc.
Microsoft ® Encarta ® 2009. © 1993-2008 Microsoft
Corporation. All rights reserved.
Starts from The Problems
Energy Crisis
The use of fossil fuels these days are very
closely related to the problem of energy crisis.
Fossil fuels are formed and stored materials in
the earth that comes from animals and
microorganisms were buried millions of years.
Based on these facts, the fossil fuel is a fuel
that can not be renewed because it requires a
very long process in its formation

3. Comes with The Ideas
Bioethanol
Bioethanol is one of the
alternative fuels that have
advantage than fossil fuel
Why Bioethanol?
Bioethanol used plants as the raw material, so
it does not make accumulation of carbon in the
atmosphere Ethanol can also increase the
efficiency of combustion because it contains
35% oxygen and environmental friendly
because dump of gas emissions such as carbon
monoxide, nitrogen oxides, and other gases are
low (19-25%)
How we make it?
During this time, bioethanol was produced
from molasses (sugar production process
waste) or starchy materials (cassava or
corn).

4. But,we face another problem
Cassava, Corn, etc.
Source of Food
find other sources of
raw materials
containing
polysaccharides and
not be used as food!
Bagasse!

5. Meet Bagasse!
Bagasse is an industrial solid waste
from sugar cane containing cellulose
fiber and usually used as a raw
material for the paper industry
Bagasse waste not yet widely used.
This waste is one of the energy
sources potential and generally is a
cellulose material that can be
converted into ethanol

6. Sugarcane Planted Area
456,700in (Ha)
spread on Sumatra, Java,
Kalimantan, and Sulawesi
tons of bagasse can produced
100each hectares in a year
Bagasse in Numbers
National potential of bagasse
4,567,0000tons each hectares in a year

7. Bioethanol
Production
Process
1st Step
2nd Step
3rd Step
Pretreatment
Process
Hydrolysis
Process
Fermentation
Process
Aimed to break down
the lignin structure
and disrupt the
crystalline structure
of cellulose
Aimed to change
glucose into
bioethanol
Aimed to change
cellulose into glucose

8. Bioethanol
Production
Process*
1st Step
2nd Step
Pretreatment
Process
Simultaneous
Saccharrification
and
Fermentation
Aimed to break down
the lignin structure
and disrupt the
crystalline structure
of cellulose
cellulose enzyme and
fermenting microbes
are added to one
vessel or hydrolysis
and fermentation
happen in one
reaction vessel can be
done by bacterium
Clostridium
thermocellum.
*with bacterium Clostridium
thermocellum

9. Fermentat
ion Tank
Distillation Purification Bioethanol
The prepared
medium/baga
sse (mashed)
Bacterium
Clostridium
thermocillum
Scheme Process of Bioethanol Production

10. Conclusion
Energy Crisis
The use of fossil fuels these days are very closely
related to the problem of energy crisis. Fossil fuels
are formed and stored materials in the earth that
comes from animals and microorganisms were
buried millions of years.
Based on these facts, the fossil fuel is a fuel that
can not be renewed because it requires a very long
process in its formation
• There are two big problems if using the
fuel oil continually, the energy crisis and
negative effect to environment such as
greenhouse effect.
• Therefore, needs a fuels alternative to
solve this problem. One of the fuel
alternative as substitute fuel oil is
bioethanol from sugarcane waste
(bagasse). It’s potential because
bagasse are abundant, so bioethanol
from bagasse can creating new high-
paying jobs, increasing market
opportunities for farmers, generating
additional household income tax and
revenues, and simultating capital
investment.
• Beside that, using bacteria Clostridium
thermocellum can make the process
bioethanol production are cheap and
fast.

11. Thank You 
Have A Nice Day!