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Bioengineering: making life from scratch Melanie Swan MS Futures Group +1-650-681-9482 [email_address] http://www.melanieswan.com Slides: http://slideshare.net/LaBlogga/slideshows NIH, Bethesda MD  September 25, 2008 Must build it to understand it
Synthetic biology Novel biological system
The new biology: virtuous feedback loop Model and simulate Investigate and  experiment Build
Bio-SPICE Biological Simulation Program  for Intra- and Inter-Cellular Evaluation http://biospice.org Model and simulate
Registry of Standard Biological Parts Build http://partsregistry.org
Parts Registry: select measurement device http://partsregistry.org
Parts Registry: obtain part sequence DNA Synthesizer http://partsregistry.org
The story of biocomputing Mapping genes to proteins -> life can be engineered Genes can be turned on and off -> gene as binary switch Systemic understanding of DNA -> genes as circuits  Cell noisiness -> need for stable and predictable circuits
Repressilator schema “ Noisy” cells Michael Elowitz Stanislas Leibler Repressilator A synthetic oscillatory network of transcriptional regulators Michael B. Elowitz & Stanislas Leibler  Nature. 2000 Jan 20;403(6767):335-38. http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
Construction of a genetic toggle switch in Escherichia coli Gardner TS, Cantor CR, Collins JJ  Nature. 2000 Jan 20;403(6767):339-42. http://www.ncbi.nlm.nih.gov/pubmed/10659857   Toggle switch induction threshold Toggle switch plasmid Gardner Cantor Collins Genetic toggle switch
Open wetware Drew Endy Parts Registry  BioBricks Foundation iGEM competition http://openwetware.org/ http://bbf.openwetware.org/ http://www.igem.org/ http://partsregistry.org
Genome  Craig Venter  Synthetic life Synthetic life Fourth-generation biofuels Mycoplasma genitalium Bacterial image Genetic map
Further applications of bioengineering Coli-roid  microbial camera Landmine sensing plant: Arabidopsis thaliana Amorphous computing Cellular colonies Natural genetic engineering Ciliate
Summary Model and simulate Investigate and  experiment Build
Thank you Melanie Swan MS Futures Group +1-650-681-9482 [email_address] http://www.melanieswan.com Slides: http://slideshare.net/LaBlogga/slideshows NIH, Bethesda MD  September 25, 2008

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Bioengineering: making life from scratch

  • 1. Bioengineering: making life from scratch Melanie Swan MS Futures Group +1-650-681-9482 [email_address] http://www.melanieswan.com Slides: http://slideshare.net/LaBlogga/slideshows NIH, Bethesda MD September 25, 2008 Must build it to understand it
  • 2. Synthetic biology Novel biological system
  • 3. The new biology: virtuous feedback loop Model and simulate Investigate and experiment Build
  • 4. Bio-SPICE Biological Simulation Program for Intra- and Inter-Cellular Evaluation http://biospice.org Model and simulate
  • 5. Registry of Standard Biological Parts Build http://partsregistry.org
  • 6. Parts Registry: select measurement device http://partsregistry.org
  • 7. Parts Registry: obtain part sequence DNA Synthesizer http://partsregistry.org
  • 8. The story of biocomputing Mapping genes to proteins -> life can be engineered Genes can be turned on and off -> gene as binary switch Systemic understanding of DNA -> genes as circuits Cell noisiness -> need for stable and predictable circuits
  • 9. Repressilator schema “ Noisy” cells Michael Elowitz Stanislas Leibler Repressilator A synthetic oscillatory network of transcriptional regulators Michael B. Elowitz & Stanislas Leibler Nature. 2000 Jan 20;403(6767):335-38. http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
  • 10. Construction of a genetic toggle switch in Escherichia coli Gardner TS, Cantor CR, Collins JJ Nature. 2000 Jan 20;403(6767):339-42. http://www.ncbi.nlm.nih.gov/pubmed/10659857 Toggle switch induction threshold Toggle switch plasmid Gardner Cantor Collins Genetic toggle switch
  • 11. Open wetware Drew Endy Parts Registry BioBricks Foundation iGEM competition http://openwetware.org/ http://bbf.openwetware.org/ http://www.igem.org/ http://partsregistry.org
  • 12. Genome Craig Venter Synthetic life Synthetic life Fourth-generation biofuels Mycoplasma genitalium Bacterial image Genetic map
  • 13. Further applications of bioengineering Coli-roid microbial camera Landmine sensing plant: Arabidopsis thaliana Amorphous computing Cellular colonies Natural genetic engineering Ciliate
  • 14. Summary Model and simulate Investigate and experiment Build
  • 15. Thank you Melanie Swan MS Futures Group +1-650-681-9482 [email_address] http://www.melanieswan.com Slides: http://slideshare.net/LaBlogga/slideshows NIH, Bethesda MD September 25, 2008