This document provides an introduction to biochemistry techniques. It discusses that biochemistry explores chemical processes in living organisms and combines principles of biology and chemistry. Some common biochemistry techniques are then listed, including homogenization, centrifugation, various types of chromatography, electrophoresis, and use of spectrophotometry and radioisotopes. The document also covers laboratory ethics, biosafety concerns like viruses and carcinogens, and the problem-solving process in scientific research.

1. BIOCHEMISTRY
TECHNIQUES
01) An Introduction
Tahaa Saeed
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2. Introduction of the Biochemistry
• Biochemistry is the branch of science that
explores the chemical processes and substances
that occur within living organisms.
• Biochemistry is considered interdisciplinary
because it combines principles and techniques
from both biology and chemistry.
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3. Biochemical Techniques
• Homogenization,
• Centrifugation,
• Paper, TLC, and column chromatography,
• Gel filtration,
• Ion exchange chromatography,
• Affinity chromatography,
• FPLC
• Electrophoresis
• UV / visible spectrophotometry
• Use of radioisotopes.
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4. Laboratory Ethics
Safety Rules and
manners
Accept that the
reality is different
than movies
Troubleshooting
the problems
Cleanliness
Laboratory
instructors
Using equipment
such as razor
blades and
scissors etc
Using
inflammable
chemicals
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5. Biosafety
Viruses and
Bacteria
The use of UV
in biochemistry
Carcinogenic
chemicals
Radioisotopes ETHIDIUM BROMIDE
IS NOT EDIBLE
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6. Solving a scientific problem
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