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Haematological tests are signifi cant diagnostic tools that are equally valuable as indicators of toxic insult or stress due to xenobiotics and environmental fluctuations. ...
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SObA WormBase Workshop International Worm Meeting 2017
This document describes SObA, a tool that generates concise graphical summaries of ontology-based annotations for genes. It summarizes extensive use of ontologies by WormBase to annotate C. elegans genes with terms related to gene function, anatomy, phenotypes, life stages and human diseases. It addresses the problem that lists of mutant phenotypes for genes with many annotations are difficult to comprehend. SObA generates interactive graphs that integrate the ontology hierarchy and logical inferences to provide a complete yet simplified view of a gene's most essential ontology annotations. It was shown to better represent the biological meaning than untrimmed lists of annotations.
II-SDV 2016 Denis Bayada - Concomitant Ontology-Driven Patent and Non-Patent ...
This document discusses using ontologies to search patent and non-patent literature in the life sciences. It explains that ontologies provide a formal structure of types, properties, and relationships between entities. When used for text searching, ontologies allow selecting a node to retrieve all related entries, but high level nodes may return too many results. Alternatively, ontology-extracted synonym lists provide flexibility without pre-computing and allow user-based curation. The document demonstrates searching CRISPR/Cas9 related literature and patents using ontologies and synonym lists in the LifeQuest system, which integrates and color codes results from different sources.
Biotecnika newspaper 16-january_2018
Living microbes have been documented for the first time in polar snow and ice samples. A new study analyzed snow samples from the University of York and found evidence of living bacteria in snowpacks through the presence of methyl iodide, a gas produced by marine bacteria. This challenges the assumption that polar ice cores are sterile and indicates that bacterial activity could be altering gas levels trapped in ice over millennia. The discovery suggests life may exist in other extreme icy environments and that ice core samples may need reevaluation to account for potential microbial impacts on trapped atmospheric gases.
Biotecnika Times Newspaper 16th Jan 2018
Living microbes have been documented for the first time in polar snow and ice samples. A new study analyzed snow samples from the University of York and found evidence of living bacteria in snowpacks through the presence of methyl iodide, a gas produced by marine bacteria. This challenges the assumption that polar ice cores are sterile and suggests microbial activity could impact the composition of ancient air trapped in ice. The discovery broadens our understanding of where life can survive in extreme environments and could affect the use of ice cores to study past climates.
Magnetic phenomenon-cv19-vials
EXHIBIT A
V A C C I N E - A N A L Y S I S - B Y - D R . R O B E R T - Y O U NG
Graphene Oxide; also known as G.O. is a compound of magnetic nanoparticles.
Seminario Molecular
This document discusses a study that used proteomic techniques like SDS-PAGE and 2D electrophoresis to identify and characterize secreted proteins from Streptococcus pneumoniae bacteria. 236 proteins were found, located in the extracellular space (21), cell wall (4), or cell membrane (31). Some proteins identified, like ZmpB and Sphtra, are highly immunogenic and potential vaccine candidates. The study provides insights into S. pneumoniae pathogenic factors and membrane proteins that could contribute to pneumococcal disease. Identifying secreted proteins through immuno-proteomic analysis may help develop effective vaccines to reduce mortality from pneumonia.
Weiland Meyer - Algae, Protists & Fungi Plenary
This document summarizes an update on DNA barcoding of human pathogenic fungi. It discusses that the ITS region has been proposed as the prime fungal barcode, but that other genetic loci like RPB1 may provide better resolution. It notes challenges with existing databases and the need for quality controlled reference databases. It outlines efforts to establish an international working group and reference database to standardize DNA barcoding for accurate identification of medically important fungi.
Coronaviruse
- Coronaviruses are enveloped viruses with RNA genomes that cause respiratory illnesses and have recently emerged to cause epidemics like SARS, MERS, and COVID-19. Chloroquine is recognized to block coronavirus infection by increasing endosome pH during virus-cell fusion and disturbing viral receptor glycosylation. While bacteria allow for scalable production, mammalian cells and plants are needed for proper protein folding and post-translational modifications important for viral targets. Recent advances prove plants can rapidly produce vaccines or biopharmaceuticals in a short time against infectious diseases.
Recommended
SObA WormBase Workshop International Worm Meeting 2017
This document describes SObA, a tool that generates concise graphical summaries of ontology-based annotations for genes. It summarizes extensive use of ontologies by WormBase to annotate C. elegans genes with terms related to gene function, anatomy, phenotypes, life stages and human diseases. It addresses the problem that lists of mutant phenotypes for genes with many annotations are difficult to comprehend. SObA generates interactive graphs that integrate the ontology hierarchy and logical inferences to provide a complete yet simplified view of a gene's most essential ontology annotations. It was shown to better represent the biological meaning than untrimmed lists of annotations.
II-SDV 2016 Denis Bayada - Concomitant Ontology-Driven Patent and Non-Patent ...
This document discusses using ontologies to search patent and non-patent literature in the life sciences. It explains that ontologies provide a formal structure of types, properties, and relationships between entities. When used for text searching, ontologies allow selecting a node to retrieve all related entries, but high level nodes may return too many results. Alternatively, ontology-extracted synonym lists provide flexibility without pre-computing and allow user-based curation. The document demonstrates searching CRISPR/Cas9 related literature and patents using ontologies and synonym lists in the LifeQuest system, which integrates and color codes results from different sources.
Biotecnika newspaper 16-january_2018
Living microbes have been documented for the first time in polar snow and ice samples. A new study analyzed snow samples from the University of York and found evidence of living bacteria in snowpacks through the presence of methyl iodide, a gas produced by marine bacteria. This challenges the assumption that polar ice cores are sterile and indicates that bacterial activity could be altering gas levels trapped in ice over millennia. The discovery suggests life may exist in other extreme icy environments and that ice core samples may need reevaluation to account for potential microbial impacts on trapped atmospheric gases.
Biotecnika Times Newspaper 16th Jan 2018
Living microbes have been documented for the first time in polar snow and ice samples. A new study analyzed snow samples from the University of York and found evidence of living bacteria in snowpacks through the presence of methyl iodide, a gas produced by marine bacteria. This challenges the assumption that polar ice cores are sterile and suggests microbial activity could impact the composition of ancient air trapped in ice. The discovery broadens our understanding of where life can survive in extreme environments and could affect the use of ice cores to study past climates.
Magnetic phenomenon-cv19-vials
EXHIBIT A
V A C C I N E - A N A L Y S I S - B Y - D R . R O B E R T - Y O U NG
Graphene Oxide; also known as G.O. is a compound of magnetic nanoparticles.
Seminario Molecular
This document discusses a study that used proteomic techniques like SDS-PAGE and 2D electrophoresis to identify and characterize secreted proteins from Streptococcus pneumoniae bacteria. 236 proteins were found, located in the extracellular space (21), cell wall (4), or cell membrane (31). Some proteins identified, like ZmpB and Sphtra, are highly immunogenic and potential vaccine candidates. The study provides insights into S. pneumoniae pathogenic factors and membrane proteins that could contribute to pneumococcal disease. Identifying secreted proteins through immuno-proteomic analysis may help develop effective vaccines to reduce mortality from pneumonia.
Weiland Meyer - Algae, Protists & Fungi Plenary
This document summarizes an update on DNA barcoding of human pathogenic fungi. It discusses that the ITS region has been proposed as the prime fungal barcode, but that other genetic loci like RPB1 may provide better resolution. It notes challenges with existing databases and the need for quality controlled reference databases. It outlines efforts to establish an international working group and reference database to standardize DNA barcoding for accurate identification of medically important fungi.
Coronaviruse
- Coronaviruses are enveloped viruses with RNA genomes that cause respiratory illnesses and have recently emerged to cause epidemics like SARS, MERS, and COVID-19. Chloroquine is recognized to block coronavirus infection by increasing endosome pH during virus-cell fusion and disturbing viral receptor glycosylation. While bacteria allow for scalable production, mammalian cells and plants are needed for proper protein folding and post-translational modifications important for viral targets. Recent advances prove plants can rapidly produce vaccines or biopharmaceuticals in a short time against infectious diseases.
Session 7: Molecular basis of response to post-harvest treatment stresses
Session 7: Molecular basis of response to post-harvest treatment stressesPlant Biosecurity Cooperative Research Centre
With the loss of chemical control options such as Fenthion and Dimethoate for postharvest treatment of horticulture commodities susceptible to fruit fly infestation, it has become even more important to understand how stress-based control techniques such as heat, cold, irradiation and bacteria parasite
can be used most effectively for disinfestation. This project aims to explore the stress-induced molecular response of two fruit fly species of horticultural significance, Mediterranean fruit fly (Ceratitis capitata) and Queensland fruit fly (Bactrocera tryoni), by characterising the cellular pathways involved in both overall and stressor-specific responses.Introdution of dna as genetic material
Frederick Griffith discovered a "transforming principle" in 1928 when live S strain bacteria developed in mice injected with a mixture of heat-killed S strain and live R strain bacteria. Later, Avery, MacLeod and McCarty determined that this transforming principle was DNA through experiments treating bacteria with enzymes that broke down DNA, RNA, and proteins. In 1952, Hershey and Chase conclusively showed through experiments with radioactive labeling of bacteriophages that DNA is the genetic material transferred from viruses to bacteria during infection, not proteins.
GRC poster
1) Researchers inoculated soil microcosms with switchgrass and tracked changes in microbial communities over 62 transfers.
2) Initial diversity decreased from 30 phyla to mainly Firmicutes (>99%) despite rare OTUs accounting for most diversity.
3) A stable yet specialized community developed, maintaining high species diversity among closely related organisms.
CORONAVIRUS AKA SARS PATENT
This patent document describes the isolation and characterization of a novel human coronavirus (SARS-CoV) that is the causative agent of severe acute respiratory syndrome (SARS). It provides the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of its open reading frames. Methods are described for using these molecules to detect SARS-CoV and detect infections. Immune stimulatory compositions are also provided, along with methods for their use.
Whats in the vax
This document discusses recent discoveries of transgenic hydra and parasites found in COVID vaccines. It claims that hydra and parasites have been genetically modified and are being used as part of a "vaccine operating system" to rewrite human genes, build an artificial neural network, and rapidly clone humans. The document outlines scientific studies it says were used to develop this system using techniques like CRISPR, mRNA, graphene oxide, and luciferase to track and control gene expression in vaccinated individuals.
Us7279327 Methods for producing recombinant coronavirus
The document is a United States patent describing methods for producing recombinant coronavirus particles. Specifically, it describes developing a helper cell that contains: 1) a coronavirus permissive cell; 2) a coronavirus replicon RNA containing a heterologous sequence and packaging signal but lacking a structural protein gene; and 3) a separate helper RNA encoding the missing structural protein. When these components are expressed in the cell, coronavirus particles are assembled that contain the heterologous RNA sequence but cannot replicate without the helper RNA. The patent claims compositions and methods for making and using these viral particles to deliver heterologous genes.
Interferon γ inhibits Ebola Virus Infection COMPAT
The document summarizes a study investigating the use of interferon gamma (IFNγ) as a potential prophylactic or therapeutic treatment for Ebola virus infection. The researchers found that IFNγ treatment made macrophages resistant to Ebola virus infection by blocking viral RNA synthesis. IFNγ stimulation in human macrophages altered the expression of over 160 genes, including 3 factors - GBP5, RARRES3, and VAMP5 - that were found to inhibit Ebola virus infection. Mouse studies showed that IFNγ reduced morbidity and mortality from Ebola virus infection and protected mice from the virus. The researchers concluded that IFNγ is a novel and effective therapeutic option for Ebola virus.
Improving methodologies for rapid diagnosis of coinfection in plants (updated...
Improving methodologies for rapid diagnosis of coinfection in plants (updated...Benjamin Schwessinger
This is a talk by Gamran Green an undergrad summer student from University of New South Wales at ANU 2016/17. Nice example of how MinION, Python, and Blast can be used to introduce students to novel technologies. Gamran didn't have any experience in plants, plant pathology, genomics, or scripting before the start of the project. Two months later this. Well done!Pulling apart the two genomes of the dikaryotic wheat stripe rust fungus
Update on our wheat stripe rust assembly with long reads. Much genetic diversity between the two nuclei.
Session 5: Exploiting hydrogen and oxygen as components of a natal origin bio...
Session 5: Exploiting hydrogen and oxygen as components of a natal origin bio...Plant Biosecurity Cooperative Research Centre
There are many facets involved in the development of biogeochemical markers that might enable the geographic origins of fruit flies to be distinguished.SMART Team Research
The Pittsburg High School SMART Team studied the crystal structure of the Ebola virus glycoprotein (GP) bound to a human survivor antibody. GP consists of two subunits, GP1 and GP2, and mediates host cell attachment and entry. The crystal structure revealed that three GP1 subunits are cradled by GP2 helices in a bowl shape, with the receptor-binding site inside. Three antibody units bind tightly to GP2 via interactions that prevent GP2 from rearranging into its fusion form, neutralizing the virus. Understanding this structure may help develop effective vaccines against Ebola by inhibiting GP's role in the viral life cycle.
Folding
New research has found that the process by which retroviruses like HIV reproduce and assemble new viruses is more complex than previously thought. Specifically, the Gag protein is transported into the cell nucleus before going to the cell membrane, where it binds to viral RNA and changes shape in response. Understanding viral reproduction is important for developing treatments for retroviral diseases like AIDS. Additionally, researchers discovered a microRNA that regulates red blood cell production, which could help treat diseases involving excessive blood cell growth like polycythemia vera.
DNA as genetic material
Frederick Griffith's experiments in 1928 showed that a transforming principle could pass from dead virulent pneumonia bacteria to live avirulent bacteria, making them deadly. Oswald Avery later purified this principle and through chemical and enzymatic tests determined it was DNA, not protein as previously believed. In 1952, Hershey and Chase used radioactive labeling to track the entry of DNA and proteins from bacteriophages into infected bacteria. They found that only the labeled DNA entered the bacterial cells, providing definitive evidence that DNA is the genetic material.
Astrovirus
Astroviruses are non-enveloped viruses with positive-sense RNA genomes that cause gastroenteritis. They have an icosahedral capsid containing three major proteins and a genome organized into three open reading frames. ORF1 encodes nonstructural proteins involved in replication, ORF2 encodes the structural capsid polyprotein which is processed by cellular proteases. Infection involves binding to an unknown receptor, translation of viral proteins, processing of polyproteins, replication through a negative-sense intermediate, and assembly of new virions which are released from the cell.
Session 7: Development of a female Q-fly lure
Research and potential impact of the development of an effective lure for Queensland Fruit fly (Q-fly), Bactrocera dorsalis.
Transforming principle..
Frederick Griffith discovered the principle of transformation through experiments with Streptococcus pneumoniae bacteria. He found that heat-killed virulent S-strain bacteria could make non-virulent R-strain bacteria transform and become virulent. Later, Oswald Avery, Colin MacLeod and Maclyn McCarty discovered that DNA alone from S bacteria could transform R bacteria. Alfred Hershey and Martha Chase provided conclusive evidence that DNA, not protein, is the genetic material through experiments using bacteriophages infecting E. coli bacteria. They found that radioactive DNA, but not protein, passed from the bacteriophages into the bacterial cells.
CRISPRs
This document provides an overview of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) including its discovery, definition, and how it works. CRISPR is a gene editing technique that uses the CRISPR system found in bacteria as a tool to edit genes. The document discusses how CRISPR works in bacteria to provide immunity against viruses and how scientists have adapted this system to edit genes in other organisms. Potential applications discussed include using CRISPR to end diseases, create designer babies, and potentially slow aging.
Detection of Gene HLA-B5801 on A Lateral-Flow Membrane
6th International Scientific Conference on Applied
Sciences and Engineering
26-27 January, 2016
Bayview Hotel Singapore
Long Living mclk1+-mice
Mclk1+/- mice, which have reduced levels of the Mclk1 gene, lived longer than normal mice. Researchers found that reducing levels of the Mclk1 gene by half extended the median lifespan of male and female mice by 13-14% compared to normal mice. This study suggests that reducing expression of the Mclk1 gene can increase longevity in mice.
dkNET Webinar "YCharOS: Antibody Characterization Through Open Science" 10/22...
Abstract
Many (most!) genes/proteins linked to disease phenotypes remain severely understudied [1]. Distinct open-science initiatives are needed to promote the exploration of currently understudied proteins, from the proper identification of research reagents [2], to the development of a chemical probes for every human protein [1]. We focus on the proper characterization of antibodies to guide researchers in selecting the most specific/selective antibodies for their needed application(s) [3].
Antibodies are among the most commonly used reagents in cell biology. Generally, scientists purchase antibodies from commercial suppliers, and rely on the vendor’s quality control data to make their purchasing decisions. While there are many outstanding commercially-available antibodies, many other antibodies do not perform as advertised - and in the absence of an objective means to compare performance, it is impossible to tell one from the other. This is a widely known problem that plagues tens of thousands of scientists annually [4-6].
There is a scientific solution, enabled by CRISPR/Cas9 technology. By comparing signals from wild-type and isogenic knockout cells, one can readily test the specificity of antibodies. We applied this approach in a pilot study demonstrating that only three of the 16 commercially-available antibodies for C9ORF72, the protein product of a major amyotrophic lateral sclerosis disease locus, specifically recognized the protein. Distressingly, neither antibody had been used in a publication, and the antibody used most frequently in publications, which have been cited thousands of times, did not recognize the protein in any application [7].
We are now applying our antibody characterization pipeline to generate head-to-head comparisons of commercial antibodies for all human proteins. This work is performed in partnership with high-quality manufacturers that provide in-kind reagents (i.e. antibodies and knock-out lines). Finalized antibody characterization reports are progressively uploaded on a free open-science repository (https://zenodo.org/communities/ycharos/). We believe our initiative, Antibody Characterization through Open Science (YCharOS), will contribute to make science more reproducible and help illuminate the dark genome.
The top 3 key questions that YCharOS can answer:
1. Do antibodies used in my field perform as advertised?
2. How do I identify the best performing antibody for my protein of interest?
3. Do I need to launch an expensive and time-consuming antibody generation study or do effective commercial antibodies already exist for my protein of interest?
References (see https://dknet.org/about/blog/2334)
Presenter: Carl Laflamme, PhD, Senior Postdoctoral Fellow at the Montreal Neurological Institute (The Neuro, McGill University). Chetan Raina, YCharOS, CEO.
Upcoming webinars schedule: https://dknet.org/about/webinar
Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indi...
"Introduction Betel quid BQ chewing, a habit practiced in Eastern and North Eastern part of India, has known to be associated with cancer of the oral or buccal cavity. BQ is also one of the common mood elevating substances among Indian population. The BQ is a mixture of areca nut Areca catechu , catechu Acacia catechu and slaked lime calcium oxide and calcium hydroxide wrapped in a betel leaf Piper betel .BQ products have been classified by the International Agency for Research on Cancer IARC as group I human carcinogens . Glutathione peroxidise GPx , one of the major enzymatic antioxidant defence system, responsible for scavenging free radicals. Antioxidant enzymes catalyze decomposition of ROS. Overall balance between production and removal of ROS may be more important in various cancers including OSCC Oral squamous cell carcinoma or oral cancer. Methods In this study subjects were screened from Department of Oral and Maxillofacial surgery andE.N.T. of Ramakrishna Mission Seva Pratishthan Hospital RKMSP , Kolkata and different areas of West Bengal and North Eastern states of India. Quantitative in vitro determination of glutathione peroxidase activities in whole blood were estimated manually with 0.05 ml whole blood. The samples were assayed by UV Visible Spectrophotometer SPECORD 50 PLUS at a wavelength of 340nm. Results Most of the subjects had betel quid chewing habit. Glutathione peroxidase values are higher in healthy control than Cancer cases and Pre cancer with betel quid chewing habit, which is statistically significant. Conclusion Reactive oxygen species are generated due to slaked lime, one of the important constituents of betel quid which can modulate the oral pathology and promote carcinogenesis. Aniket Adhikari | Madhusnata De ""Study of Glutathione Peroxidase (GPX) Activity Among Betel Quid Chewers of Indian Population"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21619.pdf
Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/21619/study-of-glutathione-peroxidase-gpx-activity-among-betel-quid-chewers-of-indian-population/aniket-adhikari"
IN-SILICO CHARACTERISATION OF PROTEIN CODED BY CYT-B GENE OF Radopholus simil...
Of the more than 30 species in the genus Radopholus, the burrowing nematode, Radopholus similis, is the only pathogen of widespread economic importance (Duncan and Moens, 2006). Radopholus similis is a migratory endoparasitic nematode that is known to be a destructive pest of citrus crops, pepper and, most importantly, banana, on which it causes toppling disease. The nematode causes economic problems throughout the world, most notably in warmer regions, including South America, the Caribbean, Africa, Asia and the Pacific.
More Related Content
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Session 7: Molecular basis of response to post-harvest treatment stresses
Session 7: Molecular basis of response to post-harvest treatment stressesPlant Biosecurity Cooperative Research Centre
With the loss of chemical control options such as Fenthion and Dimethoate for postharvest treatment of horticulture commodities susceptible to fruit fly infestation, it has become even more important to understand how stress-based control techniques such as heat, cold, irradiation and bacteria parasite
can be used most effectively for disinfestation. This project aims to explore the stress-induced molecular response of two fruit fly species of horticultural significance, Mediterranean fruit fly (Ceratitis capitata) and Queensland fruit fly (Bactrocera tryoni), by characterising the cellular pathways involved in both overall and stressor-specific responses.Introdution of dna as genetic material
Frederick Griffith discovered a "transforming principle" in 1928 when live S strain bacteria developed in mice injected with a mixture of heat-killed S strain and live R strain bacteria. Later, Avery, MacLeod and McCarty determined that this transforming principle was DNA through experiments treating bacteria with enzymes that broke down DNA, RNA, and proteins. In 1952, Hershey and Chase conclusively showed through experiments with radioactive labeling of bacteriophages that DNA is the genetic material transferred from viruses to bacteria during infection, not proteins.
GRC poster
1) Researchers inoculated soil microcosms with switchgrass and tracked changes in microbial communities over 62 transfers.
2) Initial diversity decreased from 30 phyla to mainly Firmicutes (>99%) despite rare OTUs accounting for most diversity.
3) A stable yet specialized community developed, maintaining high species diversity among closely related organisms.
CORONAVIRUS AKA SARS PATENT
This patent document describes the isolation and characterization of a novel human coronavirus (SARS-CoV) that is the causative agent of severe acute respiratory syndrome (SARS). It provides the nucleic acid sequence of the SARS-CoV genome and the amino acid sequences of its open reading frames. Methods are described for using these molecules to detect SARS-CoV and detect infections. Immune stimulatory compositions are also provided, along with methods for their use.
Whats in the vax
This document discusses recent discoveries of transgenic hydra and parasites found in COVID vaccines. It claims that hydra and parasites have been genetically modified and are being used as part of a "vaccine operating system" to rewrite human genes, build an artificial neural network, and rapidly clone humans. The document outlines scientific studies it says were used to develop this system using techniques like CRISPR, mRNA, graphene oxide, and luciferase to track and control gene expression in vaccinated individuals.
Us7279327 Methods for producing recombinant coronavirus
The document is a United States patent describing methods for producing recombinant coronavirus particles. Specifically, it describes developing a helper cell that contains: 1) a coronavirus permissive cell; 2) a coronavirus replicon RNA containing a heterologous sequence and packaging signal but lacking a structural protein gene; and 3) a separate helper RNA encoding the missing structural protein. When these components are expressed in the cell, coronavirus particles are assembled that contain the heterologous RNA sequence but cannot replicate without the helper RNA. The patent claims compositions and methods for making and using these viral particles to deliver heterologous genes.
Interferon γ inhibits Ebola Virus Infection COMPAT
The document summarizes a study investigating the use of interferon gamma (IFNγ) as a potential prophylactic or therapeutic treatment for Ebola virus infection. The researchers found that IFNγ treatment made macrophages resistant to Ebola virus infection by blocking viral RNA synthesis. IFNγ stimulation in human macrophages altered the expression of over 160 genes, including 3 factors - GBP5, RARRES3, and VAMP5 - that were found to inhibit Ebola virus infection. Mouse studies showed that IFNγ reduced morbidity and mortality from Ebola virus infection and protected mice from the virus. The researchers concluded that IFNγ is a novel and effective therapeutic option for Ebola virus.
Improving methodologies for rapid diagnosis of coinfection in plants (updated...
Improving methodologies for rapid diagnosis of coinfection in plants (updated...Benjamin Schwessinger
This is a talk by Gamran Green an undergrad summer student from University of New South Wales at ANU 2016/17. Nice example of how MinION, Python, and Blast can be used to introduce students to novel technologies. Gamran didn't have any experience in plants, plant pathology, genomics, or scripting before the start of the project. Two months later this. Well done!Pulling apart the two genomes of the dikaryotic wheat stripe rust fungus
Update on our wheat stripe rust assembly with long reads. Much genetic diversity between the two nuclei.
Session 5: Exploiting hydrogen and oxygen as components of a natal origin bio...
Session 5: Exploiting hydrogen and oxygen as components of a natal origin bio...Plant Biosecurity Cooperative Research Centre
There are many facets involved in the development of biogeochemical markers that might enable the geographic origins of fruit flies to be distinguished.SMART Team Research
The Pittsburg High School SMART Team studied the crystal structure of the Ebola virus glycoprotein (GP) bound to a human survivor antibody. GP consists of two subunits, GP1 and GP2, and mediates host cell attachment and entry. The crystal structure revealed that three GP1 subunits are cradled by GP2 helices in a bowl shape, with the receptor-binding site inside. Three antibody units bind tightly to GP2 via interactions that prevent GP2 from rearranging into its fusion form, neutralizing the virus. Understanding this structure may help develop effective vaccines against Ebola by inhibiting GP's role in the viral life cycle.
Folding
New research has found that the process by which retroviruses like HIV reproduce and assemble new viruses is more complex than previously thought. Specifically, the Gag protein is transported into the cell nucleus before going to the cell membrane, where it binds to viral RNA and changes shape in response. Understanding viral reproduction is important for developing treatments for retroviral diseases like AIDS. Additionally, researchers discovered a microRNA that regulates red blood cell production, which could help treat diseases involving excessive blood cell growth like polycythemia vera.
DNA as genetic material
Frederick Griffith's experiments in 1928 showed that a transforming principle could pass from dead virulent pneumonia bacteria to live avirulent bacteria, making them deadly. Oswald Avery later purified this principle and through chemical and enzymatic tests determined it was DNA, not protein as previously believed. In 1952, Hershey and Chase used radioactive labeling to track the entry of DNA and proteins from bacteriophages into infected bacteria. They found that only the labeled DNA entered the bacterial cells, providing definitive evidence that DNA is the genetic material.
Astrovirus
Astroviruses are non-enveloped viruses with positive-sense RNA genomes that cause gastroenteritis. They have an icosahedral capsid containing three major proteins and a genome organized into three open reading frames. ORF1 encodes nonstructural proteins involved in replication, ORF2 encodes the structural capsid polyprotein which is processed by cellular proteases. Infection involves binding to an unknown receptor, translation of viral proteins, processing of polyproteins, replication through a negative-sense intermediate, and assembly of new virions which are released from the cell.
Session 7: Development of a female Q-fly lure
Research and potential impact of the development of an effective lure for Queensland Fruit fly (Q-fly), Bactrocera dorsalis.
Transforming principle..
Frederick Griffith discovered the principle of transformation through experiments with Streptococcus pneumoniae bacteria. He found that heat-killed virulent S-strain bacteria could make non-virulent R-strain bacteria transform and become virulent. Later, Oswald Avery, Colin MacLeod and Maclyn McCarty discovered that DNA alone from S bacteria could transform R bacteria. Alfred Hershey and Martha Chase provided conclusive evidence that DNA, not protein, is the genetic material through experiments using bacteriophages infecting E. coli bacteria. They found that radioactive DNA, but not protein, passed from the bacteriophages into the bacterial cells.
CRISPRs
This document provides an overview of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) including its discovery, definition, and how it works. CRISPR is a gene editing technique that uses the CRISPR system found in bacteria as a tool to edit genes. The document discusses how CRISPR works in bacteria to provide immunity against viruses and how scientists have adapted this system to edit genes in other organisms. Potential applications discussed include using CRISPR to end diseases, create designer babies, and potentially slow aging.
Detection of Gene HLA-B5801 on A Lateral-Flow Membrane
6th International Scientific Conference on Applied
Sciences and Engineering
26-27 January, 2016
Bayview Hotel Singapore
Long Living mclk1+-mice
Mclk1+/- mice, which have reduced levels of the Mclk1 gene, lived longer than normal mice. Researchers found that reducing levels of the Mclk1 gene by half extended the median lifespan of male and female mice by 13-14% compared to normal mice. This study suggests that reducing expression of the Mclk1 gene can increase longevity in mice.
dkNET Webinar "YCharOS: Antibody Characterization Through Open Science" 10/22...
Abstract
Many (most!) genes/proteins linked to disease phenotypes remain severely understudied [1]. Distinct open-science initiatives are needed to promote the exploration of currently understudied proteins, from the proper identification of research reagents [2], to the development of a chemical probes for every human protein [1]. We focus on the proper characterization of antibodies to guide researchers in selecting the most specific/selective antibodies for their needed application(s) [3].
Antibodies are among the most commonly used reagents in cell biology. Generally, scientists purchase antibodies from commercial suppliers, and rely on the vendor’s quality control data to make their purchasing decisions. While there are many outstanding commercially-available antibodies, many other antibodies do not perform as advertised - and in the absence of an objective means to compare performance, it is impossible to tell one from the other. This is a widely known problem that plagues tens of thousands of scientists annually [4-6].
There is a scientific solution, enabled by CRISPR/Cas9 technology. By comparing signals from wild-type and isogenic knockout cells, one can readily test the specificity of antibodies. We applied this approach in a pilot study demonstrating that only three of the 16 commercially-available antibodies for C9ORF72, the protein product of a major amyotrophic lateral sclerosis disease locus, specifically recognized the protein. Distressingly, neither antibody had been used in a publication, and the antibody used most frequently in publications, which have been cited thousands of times, did not recognize the protein in any application [7].
We are now applying our antibody characterization pipeline to generate head-to-head comparisons of commercial antibodies for all human proteins. This work is performed in partnership with high-quality manufacturers that provide in-kind reagents (i.e. antibodies and knock-out lines). Finalized antibody characterization reports are progressively uploaded on a free open-science repository (https://zenodo.org/communities/ycharos/). We believe our initiative, Antibody Characterization through Open Science (YCharOS), will contribute to make science more reproducible and help illuminate the dark genome.
The top 3 key questions that YCharOS can answer:
1. Do antibodies used in my field perform as advertised?
2. How do I identify the best performing antibody for my protein of interest?
3. Do I need to launch an expensive and time-consuming antibody generation study or do effective commercial antibodies already exist for my protein of interest?
References (see https://dknet.org/about/blog/2334)
Presenter: Carl Laflamme, PhD, Senior Postdoctoral Fellow at the Montreal Neurological Institute (The Neuro, McGill University). Chetan Raina, YCharOS, CEO.
Upcoming webinars schedule: https://dknet.org/about/webinar
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Session 7: Molecular basis of response to post-harvest treatment stresses
Session 7: Molecular basis of response to post-harvest treatment stresses
Us7279327 Methods for producing recombinant coronavirus
Us7279327 Methods for producing recombinant coronavirus
Interferon γ inhibits Ebola Virus Infection COMPAT
Interferon γ inhibits Ebola Virus Infection COMPAT
Improving methodologies for rapid diagnosis of coinfection in plants (updated...
Improving methodologies for rapid diagnosis of coinfection in plants (updated...
Pulling apart the two genomes of the dikaryotic wheat stripe rust fungus
Pulling apart the two genomes of the dikaryotic wheat stripe rust fungus
Session 5: Exploiting hydrogen and oxygen as components of a natal origin bio...
Session 5: Exploiting hydrogen and oxygen as components of a natal origin bio...
Detection of Gene HLA-B5801 on A Lateral-Flow Membrane
Detection of Gene HLA-B5801 on A Lateral-Flow Membrane
dkNET Webinar "YCharOS: Antibody Characterization Through Open Science" 10/22...
dkNET Webinar "YCharOS: Antibody Characterization Through Open Science" 10/22...
Similar to assessment-of-chromium-oxide-nanoparticles-intake-in-rattus-norvegicus-by-primary-renal-function-markers-and-rbc-architecture-global-journal-of-zoology-peertechz-journals
Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indi...
"Introduction Betel quid BQ chewing, a habit practiced in Eastern and North Eastern part of India, has known to be associated with cancer of the oral or buccal cavity. BQ is also one of the common mood elevating substances among Indian population. The BQ is a mixture of areca nut Areca catechu , catechu Acacia catechu and slaked lime calcium oxide and calcium hydroxide wrapped in a betel leaf Piper betel .BQ products have been classified by the International Agency for Research on Cancer IARC as group I human carcinogens . Glutathione peroxidise GPx , one of the major enzymatic antioxidant defence system, responsible for scavenging free radicals. Antioxidant enzymes catalyze decomposition of ROS. Overall balance between production and removal of ROS may be more important in various cancers including OSCC Oral squamous cell carcinoma or oral cancer. Methods In this study subjects were screened from Department of Oral and Maxillofacial surgery andE.N.T. of Ramakrishna Mission Seva Pratishthan Hospital RKMSP , Kolkata and different areas of West Bengal and North Eastern states of India. Quantitative in vitro determination of glutathione peroxidase activities in whole blood were estimated manually with 0.05 ml whole blood. The samples were assayed by UV Visible Spectrophotometer SPECORD 50 PLUS at a wavelength of 340nm. Results Most of the subjects had betel quid chewing habit. Glutathione peroxidase values are higher in healthy control than Cancer cases and Pre cancer with betel quid chewing habit, which is statistically significant. Conclusion Reactive oxygen species are generated due to slaked lime, one of the important constituents of betel quid which can modulate the oral pathology and promote carcinogenesis. Aniket Adhikari | Madhusnata De ""Study of Glutathione Peroxidase (GPX) Activity Among Betel Quid Chewers of Indian Population"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21619.pdf
Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/21619/study-of-glutathione-peroxidase-gpx-activity-among-betel-quid-chewers-of-indian-population/aniket-adhikari"
IN-SILICO CHARACTERISATION OF PROTEIN CODED BY CYT-B GENE OF Radopholus simil...
Of the more than 30 species in the genus Radopholus, the burrowing nematode, Radopholus similis, is the only pathogen of widespread economic importance (Duncan and Moens, 2006). Radopholus similis is a migratory endoparasitic nematode that is known to be a destructive pest of citrus crops, pepper and, most importantly, banana, on which it causes toppling disease. The nematode causes economic problems throughout the world, most notably in warmer regions, including South America, the Caribbean, Africa, Asia and the Pacific.
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Coronavirus disease 2019 has brought about a great threat to global public health. More than 762 million confirmed cases have been reported according to the statistics of the World Health Organization (WHO), including over 6 million deaths. By the end of February 2023, an XBB sublineage named XBB.1.16 emerged and was detected in various countries especially India, USA and UK. XBB.1.16 had an effective reproduction number (Re) that was 1.27 and 1.17 times higher than XBB.1 and XBB.1.5, respectively. Also, XBB.1.16 is very resistant to a variety of anti-SARS-CoV-2 antibodies as well as XBB.1 and XBB.1.5. We conclude that we could face a new pandemic in the coming months.
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Coronavirus disease 2019 has brought about a great threat to global public health. More than 762 million confirmed cases have been reported according to the statistics of the World Health Organization (WHO), including over 6 million deaths. By the end of February 2023, an XBB sublineage named XBB.1.16 emerged and was detected in various countries especially India, USA and UK. XBB.1.16 had an effective reproduction number (Re) that was 1.27 and 1.17 times higher than XBB.1 and XBB.1.5, respectively. Also, XBB.1.16 is very resistant to a variety of anti-SARS-CoV-2 antibodies as well as XBB.1 and XBB.1.5. We conclude that we could face a new pandemic in the coming months
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Coronavirus disease 2019 has brought about a great threat to global public health. More than 762 million confirmed cases have
been reported according to the statistics of the World Health Organization (WHO), including over 6 million deaths. By the end
of February 2023, an XBB sublineage named XBB.1.16 emerged
and was detected in various countries especially India, USA and
UK. XBB.1.16 had an effective reproduction number (Re) that was
1.27 and 1.17 times higher than XBB.1 and XBB.1.5, respectively.
Also, XBB.1.16 is very resistant to a variety of anti-SARS-CoV-2
antibodies as well as XBB.1 and XBB.1.5. We conclude that we
could face a new pandemic in the coming months.
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Coronavirus disease 2019 has brought about a great threat to global public health. More than 762 million confirmed cases have been reported according to the statistics of the World Health Organization (WHO), including over 6 million deaths. By the end of February 2023, an XBB sublineage named XBB.1.16 emerged and was detected in various countries especially India, USA and UK. XBB.1.16 had an effective reproduction number (Re) that was 1.27 and 1.17 times higher than XBB.1 and XBB.1.5, respectively. Also, XBB.1.16 is very resistant to a variety of anti-SARS-CoV-2 antibodies as well as XBB.1 and XBB.1.5. We conclude that we could face a new pandemic in the coming months.
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Coronavirus disease 2019 has brought about a great threat to global public health. More than 762 million confirmed cases have
been reported according to the statistics of the World Health Organization (WHO), including over 6 million deaths. By the end
of February 2023, an XBB sublineage named XBB.1.16 emerged
and was detected in various countries especially India, USA and
UK. XBB.1.16 had an effective reproduction number (Re) that was
1.27 and 1.17 times higher than XBB.1 and XBB.1.5, respectively.
Also, XBB.1.16 is very resistant to a variety of anti-SARS-CoV-2
antibodies as well as XBB.1 and XBB.1.5. We conclude that we
could face a new pandemic in the coming months.
mHealth Israel_Ryo Kosaka_AIST_National Institute of Advanced Industrial Scie...
Presentation by Ryo Kosaka, Senior Research Scientist, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST). Includes an overview of priority strategies in Life Sciences and Biotech and description of the organization of the Life Sciences and Biotech department. Recent projects include a Portable System for High-Speed DNA Quantification, Application of a cell microarray chip for clinical diagnosis and single cell analysis, Safe and Secure Artificial Heart, New diagnosis for liver fibrosis utilizing glycans, AIST ventures from the department of Life Science & Biotech as well as International cooperation.
Presentation2 (1) edited (2)
This study investigated the cytotoxicity of copper oxide (CuO) nanoparticles on human blood lymphocytes. CuO nanoparticles are used in various applications like medicine, engineering, and technology. The study found that CuO nanoparticles decreased cell viability in human lymphocytes in a concentration-dependent manner. Specifically, 382μM of CuO nanoparticles caused a 50% decrease in viability. The cytotoxicity was associated with a significant increase in intracellular reactive oxygen species levels and loss of mitochondrial and lysosomal membrane integrity.
1 s2.0-s2213398421000786-main
This study analyzed hematological and biochemical markers in 249 patients hospitalized with COVID-19, of which 58 patients died. The key findings were:
1) Non-survivors had significantly higher neutrophil counts, procalcitonin, C-reactive protein, AST, serum potassium, prothrombin time, activated prothrombin time, and LDH compared to survivors.
2) Survivors had significantly higher lymphocyte counts and hemoglobin levels than non-survivors.
3) Patients with abnormal chest x-ray findings had significantly higher white blood cell counts, neutrophil counts, platelet counts, serum potassium, procalcitonin, C-reactive protein, AST, ALT
Viroids and prions
This document summarizes information about viroids and prions. It discusses viroids, which are small circular RNA molecules that infect plants and cause disease but do not encode any proteins. The potato spindle tuber viroid is described as one of the first viroids discovered that causes potato spindle tuber disease. Prions are described as unconventional infectious agents made of misfolded protein that cause transmissible spongiform encephalopathies like mad cow disease. The document also discusses how viroids and prions were discovered and researched, as well as their structures, modes of transmission, and diseases caused.
Impact of Metal Oxide Nanoparticles on Beneficial Soil Microorganisms and the...
Impact of Metal Oxide Nanoparticles on Beneficial Soil Microorganisms and the...SSR Institute of International Journal of Life Sciences
ABSTRACT- In this study, the effect of ZnO and TiO2-NPs on beneficial soil microorganisms and their secondary metabolites production was investigated. The antibacterial potential of NPs were determined by growth kinetics of P. aeruginosa, P. fluorescens and B. amyloliquefaciens. Significantly decreased in the cell viability based on optical density measurements were observed upon treatment with increasing concentrations of NPs. While comparing the effect of the different concentrations of the NPs (200 µg/ml) on IAA production by different bacterial strains, ZnO nanoparticles showed greater inhibitory effect than TiO2-NPs on IAA production by bacterial strains. The effect of Nanoparticles on phosphate solubilization was found inhibitory at 200 µg/ml. Treatment with ZnO showed concentration dependent enhancement in siderophore production by bacteriaby exposure to ZnO-NPs whereas TiO2-NPs showed concentration dependent progressive decline for iron binding siderophore molecules. Reduction in antibiotic production by P. aeruginosa and P. fluorescens was noticed in the presence of ZnO and TiO2 as compared to the control. The fluorescence of NADH released by P. aeruginosa was observed to be quenched in presence of ZnO and TiO2-NPs as compared to control. The present study highlights that the impact of nanoparticles on bacterial strains and the release of plant growth promoting substances by PGPR strains was dose dependent, which gives an idea about the level of toxicity of these nanoparticles in the environment. Therefore, the discharge of nanoparticles in the environment should be carefully monitored so that the loss of both structure and functions of agronomically important microbes could be protected from the toxicity of MO-NPs.
Key-words- MO-NPs, IAA, Phosphate Solubilization, Siderophore, PCA, NADH, ZnO-NPs, TiO2-NPs
Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Pr...
The Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence.
IJISET_V2_I2_20
This study investigated the effects of antioxidant supplementation on lipid profiles and hematological parameters in malaria-induced albino rats. Rats were infected with malaria parasites and treated with anti-malarial drugs. They were divided into three groups: Group A received antioxidants (foods), Group B received nutraceuticals, and Group C served as the control. After 12 weeks, hematological parameters like PCV, WBC, RBC, and HGB significantly increased more in Groups A and B compared to Group C. Lipid levels like TG and LDL also significantly increased more in Groups A and B versus Group C. However, CHOL and HDL levels non-significantly decreased more in Groups A and B versus Group C.
Paraquat Poisoning (Lethal Herbicide):A Rare Case Report
Paraquat Poisoning (Lethal Herbicide):A Rare Case ReportInternational Multispeciality Journal of Health
— Paraquat, chemically 1, 1' dimethyl-4-4'-bipyridinium, is a green colored herbicide. It is one of the most lethal herbicide and is hazardous in very small quantities. More than 93% of deaths by paraquat are suicidal in nature. Poisoning by paraquat is commoner in developing countries because of agriculture based economy. The presentation of paraquat poisoning varies from case to case and depends on the amount consumed. As the compound is highly lethal, an early suspicion & management is must to prevent complications. Present case is of paraquat poisoning with full blown early picture of mucosal ulceration, pneumonitis and acute kidney injury which later on complicated in esophageal perforation, pneumothorax and pneumomediastinum.Protective effect of garlic extract against maternal and fetal cerebellar dam...
Protective effect of garlic extract against maternal and fetal cerebellar dam...Prof. Hesham N. Mustafa
Background: In spite of its industrial usefulness and varied daily uses, lead (Pb) pollution is a widespread ecological problem that faces the humans in the 21th century. Pb was found to produces a wide range of toxic effects including neurotoxicity especially to the developing and young offspring. Recently, the utilisation of herbal plants has received a significant attention where there has been rising awareness in their therapeutic use; among these is the garlic. In light of the above, the current study is designed experimentally in female pregnant rats in order to investigate the beneficial role of garlic extract in the protection from the maternal and foetal cerebellar damage produced by administration of different doses of Pb during pregnancy.
Materials and methods: Positively pregnant female rats were divided into five groups; one control group, two Pb-treated groups (exposed to 160 and 320 mg/kg b.w. of Pb, respectively) and two groups treated with both Pb and garlic (exposed to Pb as previous groups together with 250 mg/kg b.w./day of garlic extract). Treatments started from day 1 to day 20 of pregnancy, where the mother rats of different experimental groups were sacrificed to obtain the foetuses. Pb level in the maternal and foetal blood and cerebellum was estimated by spectrophotometry. Specimens of the cerebellum of different mother and foetal groups were processed to histological and immunohistochemical staining for microscopic examination.
Results: The results showed that administration of Pb to pregnant rats resulted in a dose-dependent toxicity for both mothers and foetuses in the form of decrease in maternal weight gain, placental and foetal weights, brain weight and diminished foetal growth parameters, which were prominent in rat's group treated with larger dose of Pb. In Pb-treated rats, Pb level in blood and cerebellum was high when compared with the control group. The histopathological examination of the cerebellum of treated dams and foetuses showed marked alterations mainly in the form of Purkinje cell degeneration and lack of development of foetal cerebellum. Co-treatment of garlic extract along with Pb resulted in a significant decrease in Pb levels as compared with those treated with Pb alone with improvement of the histopathological changes.
Conclusions: This study was useful in evaluating the hazardous effects of uncontrolled use of Pb in general and in assessing the developmental and neurotoxicity of foetuses due to exposure during pregnancy in particular. Co-administration of garlic has beneficial effects in amelioration of Pb-induced neurotoxicity and reversing the histopathological changes of the cerebellum of mother rats and foetuses. (Folia Morphol 2018; 77, 1: 1-15).
Keywords: Purkinje cells; garlic; glial fibrillary acidic protein; lead.Study of Biocidal Activity of Copper A Review
Copper ions, either alone or in copper complexes, have been used to disinfect liquids, solids and human tissue for centuries. Today copper is used as a water purifier, algaecide, fungicide, nematocide, molluscicide as well as an anti bacterial and anti fouling agent. Copper also displays potent anti viral activity. We have explained i the biocidal properties of copper ii the possible mechanisms by which copper is toxic to microorganisms and iii the systems by which many microorganisms resist high concentrations of heavy metals, with an emphasis on copper. Health care associated infections HAIs are a global problem associated with significant morbidity and mortality. Controlling the spread of antimicrobial resistant bacteria is a major public health challenge, and antimicrobial resistance has become one of the most important global problems in current times. The antimicrobial effect of copper has been known for centuries, and ongoing research is being conducted on the use of copper coated hard and soft surfaces for reduction of microbial contamination and, subsequently, reduction of HAIs. Dr. Pragya Tank "Study of Biocidal Activity of Copper: A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-6 | Issue-5 , August 2022, URL: https://www.ijtsrd.com/papers/ijtsrd50696.pdf Paper URL: https://www.ijtsrd.com/chemistry/other/50696/study-of-biocidal-activity-of-copper-a-review/dr-pragya-tank
Snake Envenomation Case Report
Snake bite should always be considered as a medical emergency. Snake venoms are categorised based on different types of toxic components present in it. Venomous snake bite can affect nervous system as well as normal hemostasis . The most important thing to do for a snake bite is to get emergency medical help as soon as possible. The goals of pharmacotherapy are to neutralize the toxin, to reduce morbidity, and to prevent complications. Blessy Rachal Boban | Cillamol K. J | Elena Cheruvil | Sheffin Thomas | Tony Abraham ""Snake Envenomation - Case Report"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-4 , June 2019, URL: https://www.ijtsrd.com/papers/ijtsrd24037.pdf
Paper URL: https://www.ijtsrd.com/medicine/other/24037/snake-envenomation---case-report/blessy-rachal-boban
Aquaculture microbiology and biotechnology vol (1)
This chapter discusses transgenic fish and the applications of genetic engineering in aquaculture. Specifically, it describes how genetic engineering techniques like chromosome manipulation and hormone treatment are currently used to produce sterile and monosex fish lines. It also outlines how researchers are using transgenic methods to develop fish with desirable traits like increased growth rates, improved feed conversion efficiency, disease resistance, and tolerance to stressful environmental conditions. However, the release of transgenic fish into the environment has raised ecological and human health concerns from some groups. The chapter examines both the potential benefits of transgenic fish for aquaculture as well as some of the criticisms against this technology.
Development of Dot-blot Hybridization Based on 522 bp Repetitive Sequence (R5...
Development of Dot-blot Hybridization Based on 522 bp Repetitive Sequence (R5...Tenri Ashari Wanahari
Toxoplasmosis, arising from infection by Toxoplasma gondii, is one of the most common parasitic diseases in humans and other warm-blooded animals. In humans, infections are usually asymptomatic but severe disease can occur in immunocompromized individuals and newborns. Due to the importance of the disease and in order to take suitable measures, an early diagnosis of the disease is essential, particularly for pregnant women and in industry of domestic animals. The genome of T. gondii contains repeat sequences B1 and R522 which constitute ideal targets for genome-based detection methods. The 522 base pairs repeat sequences R522 are the most promising due to the high copy number, evaluated to be 200 to 300 units within the genome. We developed a simple dot-blot hybridization based on R522 sequences. The method is simple and does not require sophisticated devices. The test of the method, using cloned R522 as target, showed that the parasite detection method was sensitive and proved to be promising for use in routine health controls as well as for the survey of Toxoplasma infections.
Key words: DIG-probe, dot blot hybridization, repeat sequences, R522, Toxoplasma gondii.Similar to assessment-of-chromium-oxide-nanoparticles-intake-in-rattus-norvegicus-by-primary-renal-function-markers-and-rbc-architecture-global-journal-of-zoology-peertechz-journals (20)
Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indi...
Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indi...
IN-SILICO CHARACTERISATION OF PROTEIN CODED BY CYT-B GENE OF Radopholus simil...
IN-SILICO CHARACTERISATION OF PROTEIN CODED BY CYT-B GENE OF Radopholus simil...
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
Future Outbreak of Caused by SARS-Cov2 Omicron XBB.1.16 Variant: A Perspective
mHealth Israel_Ryo Kosaka_AIST_National Institute of Advanced Industrial Scie...
mHealth Israel_Ryo Kosaka_AIST_National Institute of Advanced Industrial Scie...
Impact of Metal Oxide Nanoparticles on Beneficial Soil Microorganisms and the...
Impact of Metal Oxide Nanoparticles on Beneficial Soil Microorganisms and the...
Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Pr...
Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Pr...
Paraquat Poisoning (Lethal Herbicide):A Rare Case Report
Paraquat Poisoning (Lethal Herbicide):A Rare Case Report
Protective effect of garlic extract against maternal and fetal cerebellar dam...
Protective effect of garlic extract against maternal and fetal cerebellar dam...
Aquaculture microbiology and biotechnology vol (1)
Aquaculture microbiology and biotechnology vol (1)
Development of Dot-blot Hybridization Based on 522 bp Repetitive Sequence (R5...
Development of Dot-blot Hybridization Based on 522 bp Repetitive Sequence (R5...
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The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Equivariant neural networks and representation theory
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Deep Software Variability and Frictionless Reproducibility
Deep Software Variability and Frictionless ReproducibilityUniversity of Rennes, INSA Rennes, Inria/IRISA, CNRS
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
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bordetella pertussis.................................ppt
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Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
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Micronuclei test.M.sc.zoology.fisheries.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
EWOCS-I: The catalog of X-ray sources in Westerlund 1 from the Extended Weste...
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
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assessment-of-chromium-oxide-nanoparticles-intake-in-rattus-norvegicus-by-primary-renal-function-markers-and-rbc-architecture-global-journal-of-zoology-peertechz-journals
- 1. Global Journal of Zoology Peertechz Journals
- 2. Research Article Assessment of Chromium Oxide Nanoparticles Intake in Rattus norvegicus by Primary Renal Function Markers and RBC Architecture Ravish Fatima and Riaz Ahmad* *Corresponding author: Riaz Ahmad, Doctor, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002 (UP), India, Tel: +91-571- 2700920/3445; Dates: Received: 22 December, 2016;; Accepted: 18 February, 2017 Published: 21 February, 2017 Citation: Fatima R, Ahmad R (2017) Assessment of Chromium Oxide Nanoparticles Intake in Rattus norvegicus by Primary Renal Function Markers and RBC Architecture. Glob J Zool 2(1): 008-012.
- 3. Abstract B. microti is known as the main etiological agent of human babesiosis and there are some case studies for that disease, highlighting the fact that this is an important “emerging tick-borne disease”. However a lot of information about this protist is unclear. The reactions in the liver are noticeable already after the fi rst three weeks of infection. This therefore provides the basis for discussion of the effect of B. microti contact with hepatocytes in vitro and in vivo. This issue is an essential objective of our study.
- 4. Thank you For more information Read Online Article… https://www.peertechz.com/articles/assessment-of- chromium-oxide-nanoparticles-intake-in-rattus-norvegicus-by- primary-renal-function-markers-and-rbc-architecture.pdf For Full Article PDF