Presentation by Ryo Kosaka, Senior Research Scientist, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST). Includes an overview of priority strategies in Life Sciences and Biotech and description of the organization of the Life Sciences and Biotech department. Recent projects include a Portable System for High-Speed DNA Quantification, Application of a cell microarray chip for clinical diagnosis and single cell analysis, Safe and Secure Artificial Heart, New diagnosis for liver fibrosis utilizing glycans, AIST ventures from the department of Life Science & Biotech as well as International cooperation.

1. Ryo Kosaka, Ph.D (Dr. Eng.)
Senior Research Scientist
Health Research Institute
National Institute of Advanced Industrial Science and Technology (AIST)

2. 2
The National Institute of Advanced Industrial
Science and Technology (AIST), one of the
largest public research organizations in Japan,
focuses on the creation and practical
realization of technologies useful to Japanese
industry and society, and on “bridging” the
gap between innovative technological seeds
and commercialization.
Extensive Research Fields 10 Branches in Japan
Researchers ･･････････ 2,316
PD & Technical stuffs･･･ 1,923
Visiting Researchers････ 4,716
Administrative employees
･････････ 696
Staff（2017.4.1）
About AIST
Energy &
Environment
Life Sci. &
Biotech.
Information
Tech. & Human
Factors
Materials &
Chemistry
Electronics &
Manufacturing
Geological
Survey of Japan
National
Metrology Inst.
of Japan

3. Priority strategies in Life Sci. & Biotech.
Diagnostic with
biomarker
Optimization
of drug
discovery
Drug
candidate
prospecting
Drug
Discovery
Bio-
production
Health and
medical care
Stem cell
engineering
Health
assessment
Medical
device
Recombinant
Plant
Recombinant
microorganism
Bio-
prospecting

4. Biomedical Res. Inst.
Molecular Profiling
Res. Ctr. for Drug Discovery
Bioproduction Res. Inst.
Health Res. Inst.
Biotechnology Res.
Inst. for Drug Discovery
Research Units: Research Institute and Research Center
Development of a novel diagnosis system for
malaria elimination NHK World: 2014/2/04
Probe to detect undeveloped iPS cells alive
On the market: 2013/3
POCT chip using paper, film and tape
BioJapan2015: 2015/10
(15)
(103)
(44)
(35)
(55)
Generation of stomach tissue from mouse
embryonic stem cells Nat Cell Biol: 2015/8
Two-armed Lab-droid “Mahoro”
AIST venture certification:2015/7
A Canine Medicine Derived from GM Plants
On the market: 2014/3
Health&
MedicalCare
Bio-
production
Drugdiscovery
AIST Tokyo Waterfront
AIST Tsukuba & Tokyo Waterfront
AIST Tsukuba & Kansai
AIST Tsukuba & Shikoku
AIST Tsukuba & Hokkaido
Low-allergy egg by genome editing
Scieitific Reports: 2016/4
Mechanism of methane production from coal
by microbes Science: 2016/10
# of reseachers 267（Jan 2017）
Organization of Dept. of Life Sci & Biotech

5. Portable System for High-Speed DNA Quantification
●On-site detection for microorganisms using a portable system.
●Only 6 minutes to quantify the targets by a rapid PCR in a microchannel.
●Specific and high-sensitive detection for target bacteria and viruses.
（AIST start-up venture）
Sample
Sample
Heating Cooling
Metal
block
controling
temp.
Microchannel
High temp.
Low temp.
Low temp. block
High temp. block
General method: by heating-cooling of metal block
AIST method: heating-cooling
by moving sample
Kyorin Pharm Co.,
Ltd.
Press release
2017/6
Speed x10
Weight 1/10
Battery-drive

6. ●Detection of target cell among the numerous cells.
●Detection of target cell with multi staining.
●Recovery and genetic analysis of target cell.
Application of a cell microarray chip for clinical diagnosis
and single cell analysis
Detection and analysis of target cell at single cell level
Malaria elimination through the development of diagnosis system. Developed diagnosis
system (A). From research work in Japan (B) to field test in Africa (C, D). Malaria-infected
erythrocytes can be detected (E, yellow arrow).
Nucleus
Staining

7. Safe and Secure Artificial Heart
Smart and sensorized artificial heart for patients
Artificial Heart
Impeller Hydrodynamic
bearing
Outlet
Inlet
Evaluation for
hemocompatibility
In-vitro
hemocompatibility
test using animal
blood
Animal Trial
Evaluation for hemolysis and
thrombogenicity
casing
Impeller Tongue
Evaluation for
mechanical issues
CFD (Computer
Fluid dynamics)
PIV(Particle
Image Velocity)
Endurance
test under
Pulsatile
flow
Design and evaluation methods
Measurement
technics
Miniaturized blood flowmeter
Real-time thrombus monitor
Measurement technics for a
patient and a device
Flowmeter using
centrifugal force
generated in a curved
cannula
Real-time thrombus
imaging Inside a
blood pump using
NIR light
IoT

8. ●A simple and rapid blood test for diagnosis of the liver fibrosis
progression (at which stages you are) in chronic hepatitis .
●World’s first assay for liver fibrosis using a glyco-biomarker and
approved by PMDA.
New diagnosis for liver fibrosis utilizing glycans
Blood test for diagnosis of the liver fibrosis that replaces biopsy
AIST developed a key technology to
find the changes of glycochain
Progression
of fibrosis
detection
Abnormal
glycochain
glycochain
Infection Hepatitis Cirrhosis Cancer
Onset 5-15 20 20-30
Years
a few days & bleeding risk >30m & safer
Progression of liver fibrosis
Biopsy Blood Test

9. GlycoBiomarker
Leading Innovation
Co. Ltd.
Glycan-related clinical
diagnostics and
pharmaceutical products
2015/4/1
MeSCue Inc.
Cell processing, R&D for
tissue therapy and
consulting for safety and
facility operation for
regenerative medicine
2016/3/7
Lifesem Inc.
Equipment for living cell
observation by scanning
electron microscope
(SEM)
2012/10/1
miRacure
Inc.
R&D and practical
application of RNA
medicine, stem cell
therapy, antibody
medicine and DDS
2016/11/4
-2013 2015 2016
Award for
excellence at the
7th Robot Award
Robotic Biology
Institute Inc.
Total lab management
system by LabDroid
Maholo 2015/7/6
TOKIWA-
Bio Inc.
Gene-cell therapies,
vectors for regenerative
medicine, and biologics
manufacture
2015/3/20
2014
jTAS Inc.
Superfast mobile system
for gene diagnostics
2015/1/23
AIST ventures from Dept. of Life Sci & Biotech
Exit 2017/6

10. International cooperation
Joint lab (DAILAB) with
DBT (India)
in bio medicine
MOU with BPPT
(Indonesia)
in health, food, and
agriculture
MOU with TISTR
(Thailand)
in food and health
MOU with SJTU
(China)
in bio medicine
Asian
Cooperation
MOU with NIST (USA)
in standard technology
Standardization strategyStandardization strategy
MOU with CNRS (France)
in standard technology
In 2016, dispatch
2 researchers
Joint lab, collaboration, personnel exchange and genetic resources exchange
DBT-AIST Int. Lab. for
Adv. Biomed.(DAILAB)
AIST 1
India 4
Sri Lanka 1
Standardization activities/joint researches
・Comprehensive research partnership 34
・Specific research partnership 33